Browsing by Subject "amastigotes"
Now showing items 1-10 of 11
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Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
(Instituto Oswaldo Cruz, Ministério da Saúde, 2007-09-01)The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from ...
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A century of research: what have we learned about the interaction of Trypanosoma cruzi with host cells?
(Instituto Oswaldo Cruz, Ministério da Saúde, 2009-07-01)Since the discovery of Trypanosoma cruzi and the brilliant description of the then-referred to new tripanosomiasis by Carlos Chagas 100 years ago, a great deal of scientific effort and curiosity has been devoted to ...
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Cloning and characterisation of a cysteine proteinase gene expressed in amastigotes of Leishmania (L.) amazonensis
(Elsevier B.V., 2003-04-01)The present study describes the cloning and characterisation of a gene encoding a cysteine proteinase isoform, Llacys1, expressed in amastigote forms of Leishmania (L.) amazonensis. Recombinant clones containing the Llacys1 ...
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Cross-priming of long lived protective CD8(+) T cells against Trypanosoma cruzi infection: Importance of a TLR9 agonist and CD4(+) T cells
(Elsevier B.V., 2007-08-10)We recently described that vaccination of mice with a gluthatione S transferase fusion protein representing amino acids 261-500 of the Amastigote Surface Protein-2 efficiently cross-primed protective CD8(+) T cells against ...
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Identification of a 30 kDa antigen from Leishmania (L.) chagasi amastigotes implicated in protective cellular responses in a murine model
(Elsevier B.V., 2000-04-24)An antigen of apparent molecular mass of 30 kDa, termed p30, was purified from Leishmania (L.) chagasi amastigotes after separation of parasite extracts by sodium dodecyl sulfate-polyacrylamide eel eletroctrophoresis ...
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Immunologically relevant strain polymorphism in the Amastigote Surface Protein 2 of Trypanosoma cruzi
(Elsevier B.V., 2007-07-01)Several evidences suggest that the Amastigote Surface Protein-2 (ASP-2) of Trypanosonia cruzi is an important target for immunity during infection. Based on this, we considered it important to evaluate its strain polymorphism. ...
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Inhibition of Leishmania (Leishmania) amazonensis growth and infectivity by aureobasidin A
(Oxford Univ Press, 2007-03-01)Objectives: To study the effect of aureobasidin A, an inhibitor of inositol phosphorylceramide (IPC) synthase, on Leishmania growth and infectivity.Methods: Effects of aureobasidin A were determined for: (i) promastigote ...
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Invasion of MDCK epithelial cells with altered expression of Rho GTPases by Trypanosoma cruzi amastigotes and metacyclic trypomastigotes of strains from the two major phylogenetic lineages
(Elsevier B.V., 2004-04-01)In order to invade mammalian cells, Trypanosoma cruzi infective forms cause distinct rearrangements of membrane and host cell cytoskeletal components. Rho GTPases have been shown to regulate three separate signal transduction ...
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Involvement of host cell heparan sulfate proteoglycan in Trypanosoma cruzi amastigote attachment and invasion
(Cambridge Univ Press, 2011-04-01)Cell surface glycosaminoglycans (GAGs) play an important role in the attachment and invasion process of a variety of intracellular pathogens. We have previously demonstrated that heparan sulfate proteoglycans (HSPG) mediate ...
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Mammalian cell invasion and intracellular trafficking by Trypanosoma cruzi infective forms
(Academia Brasileira de Ciências, 2005-03-01)Trypanosoma cruzi, the etiological agent of Chagas disease, occurs as different strains or isolates that may be grouped in two major phylogenetic lineages: T. cruzi I, associated with the sylvatic cycle and T. cruzi II, ...
