Browsing by Subject "Stem cells"

Now showing items 1-10 of 12

    • Comparação entre membrana amniótica com e sem epitélio como substrato para cultura de células epiteliais do limbo ex vivo 

      Covre, Joyce Luciana [UNIFESP]; Loureiro, Renata Ruoco [UNIFESP]; Cristovam, Priscila Cardoso [UNIFESP]; Ricardo, José Reinaldo da Silva [UNIFESP]; Freymüller-Haapalainen, Edna [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP] (Conselho Brasileiro de Oftalmologia, 2011-04-01)
      PURPOSE: To evaluate the efficacy and ultrastructural aspects of human limbal epithelial cells cultured on amniotic membrane (AM) with and without epithelium. METHODS: Limbal epithelial cell cultures were established from ...

    • Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation 

      Barros, Jeison de Nadai [UNIFESP]; Santos, Myrna Serapião dos [UNIFESP]; Barreiro, Telma Regina Maria Pereira [UNIFESP]; Belfort, Rubens Junior [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP] (Conselho Brasileiro de Oftalmologia, 2011-08-01)
      PURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for ...

    • Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation 

      Skaff, M. [UNIFESP]; Pinto, E.r.s. [UNIFESP]; Leite, Kátia Ramos Moreira [UNIFESP]; Almeida, F.g. [UNIFESP] (Sociedade Brasileira de Urologia, 2012-02-01)
      OBJECTIVE: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. MATERIALS AND METHODS: A total of 40 New Zealand male rabbits, ...

    • Early modulation of inflammation by mesenchymal stem cell after acute kidney injury 

      Semedo, Patricia [UNIFESP]; Palasio, Carolina G. [UNIFESP]; Oliveira, Cassiano D. [UNIFESP]; Feitoza, Carla Q. [UNIFESP]; Goncalves, Giselle M. [UNIFESP]; Cenedeze, Marcos Antonio [UNIFESP]; Wang, Pamella M. H. [UNIFESP]; Teixeira, Vicente P. A.; Reis, Marlene A.; Pacheco-Silva, Alvaro [UNIFESP]; Saraiva Camara, Niels Olsen [UNIFESP] (Elsevier B.V., 2009-06-01)
      Therapy with stem cells has showed to be promising for acute kidney injury (AKI), although how it works is still controversial. Modulation of the inflammatory response is one possible mechanism. Most of published data ...

    • Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector 

      Oliveira, Lauro Augusto De [UNIFESP]; Kim, Charles; Sousa, Luciene Barbosa De [UNIFESP]; Schwab, Ivan R.; Rosenblatt, Mark I. (Conselho Brasileiro de Oftalmologia, 2010-10-01)
      PURPOSE: To evaluate the transfer of heterologous genes carrying a Green Fluorescent Protein (GFP) reporter cassette to primary corneal epithelial cells ex vivo. METHODS: Freshly enucleated rabbit corneoscleral tissue was ...

    • Importância do co-cultivo com fibroblastos de camundongo 3T3 para estabelecer cultura de suspensão de células epiteliais do limbo humano 

      Cristovam, Priscila Cardoso [UNIFESP]; Glória, Maria Aparecida da [UNIFESP]; Melo, Gustavo Barreto de [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP] (Conselho Brasileiro de Oftalmologia, 2008-10-01)
      PURPOSE: To evaluate the importance of the presence of 3T3 fibroblasts for establishing limbal epithelial cultures from cell suspension obtained from corneo-scleral rims (CSR). METHODS: Corneo-scleral rims from different ...

    • Impression cytology and in vivo confocal microscopy in corneas with total limbal stem cell deficiency 

      Araújo, Aline Lütz de [UNIFESP]; Ricardo, José Reinaldo da Silva [UNIFESP]; Sakai, Vivian Naomi [UNIFESP]; Barros, Jeison de Nadai [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP] (Conselho Brasileiro de Oftalmologia, 2013-10-01)
      PURPOSES: To describe corneal changes seen on in vivo confocal microscopy in patients with total limbal stem cell deficiency and to correlate them with cytological findings. METHODS: A prospective case series including 13 ...

    • A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer 

      Hu, Yuntao; Liu, Laura; Lu, Bo; Zhu, Danhong; Ribeiro, Ramiro; Diniz, Bruno [UNIFESP]; Thomas, Padmaja B.; Ahuja, Ashish K.; Hinton, David R.; Tai, Yu-Chong; Hikita, Sherry T.; Johnson, Lincoln V.; Clegg, Dennis O.; Thomas, Biju B.; Humayun, Mark S. (Karger, 2012-01-01)
      Objective: To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal ...

    • P27(Kip1) overexpression regulates IL-1 beta in the microenvironment of stem cells and eutopic endometriosis co-cultures 

      Gonçalves, Giovana Aparecida [UNIFESP]; Invitti, Adriana Luckow [UNIFESP]; Parreira, Rafael Martins [UNIFESP]; Kopelman, Alexander [UNIFESP]; Schor, Eduardo [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP] (Academic Press Ltd- Elsevier Science Ltd, 2017)
      Endometriosis is a gynecological benign chronic disease defined as the growth of endometrial glands and stroma in extra-uterine sites, most commonly implanted over visceral and peritoneal surfaces within the female pelvis ...

    • Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells 

      Bryant, L. Henry, Jr.; Kim, Saejeong J.; Hobson, Matthew; Milo, Blerta; Kovacs, Zsofia I.; Jikaria, Neekita; Lewis, Bobbi K.; Aronova, Maria A.; Sousa, Alioscka A. {UNIFESP}; Zhang, Guofeng; Leapman, Richard D.; Frank, Joseph A. (Elsevier Science Bv, 2017)
      Stem cell-based therapies have become a major focus in regenerative medicine and to treat diseases. A straightforward approach combining three drugs, heparin (H), protamine (P) with ferumoxytol (F) in the form of nanocomplexes ...