Now showing items 1-5 of 5

    • Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism 

      Hughes, Ashley J.; Rudd, Timothy R.; Wright, Gareth S. A.; Fernig, David G.; Siligardi, Giuliano; Yates, Edwin A. [UNIFESP] (Royal Soc Chemistry, 2015-01-01)
      Presented herein is a novel method for the detection of tryptophan-ligand interactions utilising magnetic circular dichroism. Two examples are presented, one a small molecule and another, a large polysaccharide, heparan ...
    • Enhanced Tumorigenic Potential of Colorectal Cancer Cells by Extracellular Sulfatases 

      Vicente, Carolina M. [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Yates, Edwin A. [UNIFESP]; Nader, Helena B. [UNIFESP]; Toma, Leny [UNIFESP] (Amer Assoc Cancer Research, 2015-03-01)
      Heparan sulfate endosulfatase-1 and -2 (SULF1 and SULF2) are two important extracellular 6-O-endosulfatases that remove 6-O sulfate groups of N-glucosamine along heparan sulfate (HS) proteoglycan chains often found in the ...
    • F-19 labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy 

      Lima, Marcelo A. [UNIFESP]; Cavalheiro, Renan P. [UNIFESP]; Viana, Gustavo M. [UNIFESP]; Meneghetti, Maria C. Z. [UNIFESP]; Rudd, Timothy R.; Skidmore, Mark A.; Powell, Andrew K.; Yates, Edwin A. [UNIFESP] (Springer, 2017)
      Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues ...
    • Heparin prevents Zika virus induced-cytopathic effects in human neural progenitor cells 

      Ghezzi, Silvia; Cooper, Lynsay; Rubio, Alicia; Pagani, Isabel; Capobianchi, Maria Rosaria; Ippolito, Giuseppe; Pelletier, Julien; Meneghetti, Maria Cecilia Z. [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Skidmore, Mark A.; Broccoli, Vania; Yates, Edwin A. [UNIFESP]; Vicenzi, Elisa (Elsevier Science Bv, 2017)
      The recent Zika virus (ZIKV) outbreak, which mainly affected Brazil and neighbouring states, demonstrated the paucity of information concerning the epidemiology of several flaviruses, but also highlighted the lack of ...
    • On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+ 

      Córdula, Carolina Ribeiro [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Shinjo, Samuel K. [UNIFESP]; Gesteira, Tarsis F. [UNIFESP]; Pol-Fachin, Laercio; Coulson-Thomas, Vivien Jane [UNIFESP]; Verli, Hugo; Yates, Edwin A. [UNIFESP]; Rudd, Timothy R.; Pinhal, Maria A. S. [UNIFESP]; Toma, Leny [UNIFESP]; Dietrich, Carl P. [UNIFESP]; Nader, Helena B. [UNIFESP]; Tersariol, Ivarne L. S. [UNIFESP] (Royal Soc Chemistry, 2014-01-01)
      The structurally diverse polysaccharide lyase enzymes are distributed from plants to animals but share common catalytic mechanisms. One, heparinase I (F. heparinum), is employed in the production of the major anticoagulant ...