Now showing items 1-15 of 15

    • Active site mapping of Loxosceles phospholipases D: Biochemical and biological features 

      Vuitika, L.; Chaves-Moreira, D.; Caruso, I.; Lima, M. A. [UNIFESP]; Matsubara, F. H.; Murakami, M. T.; Takahashi, H. K. [UNIFESP]; Toledo, M. S. [UNIFESP]; Coronado, M. A.; Nader, H. B. [UNIFESP]; Senff-Ribeiro, A.; Chaim, O. M.; Arni, R. K. C; Veiga, S. S. (Elsevier Science Bv, 2016)
      Brown spider phospholipases D from Loxosceles venoms are among the most widely studied toxins since they induce dermonecrosis, triggering inflammatory responses, increase vascular permeability, cause hemolysis, and renal ...
    • Brown spider dermonecrotic toxin directly induces nephrotoxicity 

      Chaim, O. M.; Sade, Y. B.; Silveira, R. B. da; Toma, L.; Kalapothakis, E.; Chavez-Olortegui, C.; Mangili, O. C.; Gremski, W.; von Dietrich, C. P.; Nader, H. B.; Veiga, S. S. (Elsevier B.V., 2006-02-15)
      Brown spider (Loxosceles genus) venom can induce dermonecrotic lesions at the bite site and systemic manifestations including fever, vomiting, convulsions, disseminated intravascular coagulation, hemolytic anemia and acute ...
    • Cellular prion protein binds laminin and mediates neuritogenesis 

      Graner, E.; Mercadante, A. F.; Zanata, S. M.; Forlenza, O. V.; Cabral, ALB; Veiga, S. S.; Juliano, M. A.; Roesler, R.; Walz, R.; Minetti, A.; Izquierdo, I; Martins, V. R.; Brentani, R. R. (Elsevier B.V., 2000-03-10)
      Laminin (LN) plays a major role in neuronal differentiation, migration and survival. Here, we show that the cellular prion protein (PrPc) is a saturable, specific, high-affinity receptor for LN. the PrPc-LN interaction is ...
    • Characterization of Brown spider (Loxosceles intermedia) hemolymph: Cellular and biochemical analyses 

      Bednaski, A. V.; Trevisan-Silva, D.; Matsubara, F. H.; Boia-Ferreira, M.; Oliverio, M. M.; Gremski, L. H.; Cavalheiro, R. P. [UNIFESP]; De Paula, D. M. B. [UNIFESP]; Paredes-Gamero, E. J. [UNIFESP]; Takahashi, H. K. [UNIFESP]; Toledo, M. S. [UNIFESP]; Nader, H. B. [UNIFESP]; Veiga, S. S.; Chaim, O. M.; Senff-Ribeiro, A. (Elsevier B.V., 2015-05-01)
      This is the first study on the hemolymph from a spider of the Loxosceles genus. These animals are responsible for a great number of envenomation cases worldwide. Several studies on Loxosceles venoms have been published, ...
    • Cytotoxic, thrombolytic and edematogenic activities of leucurolysin-a, a metalloproteinase from Bothrops leucurus snake venom 

      Gremski, L. H.; Chaim, O. M.; Paludo, K. S.; Sade, Y. B.; Otuki, M. F.; Richardson, M.; Gremski, W.; Sanchez, E. F.; Veiga, S. S. (Elsevier B.V., 2007-07-01)
      Leucurolysin-a (leuc-a), a 23 kDa non-hemorrhagic metalloproteinase, is found in venom of the viper Bothrops leucurus. Here, we examine the biological consequences of leuc-a, including thrombolytic activity, direct effects ...
    • Effect of brown spider venom on basement membrane structures 

      Veiga, S. S.; Feitosa, L.; Santos, VLP dos; Souza, G. A. de; Ribeiro, A. S.; Mangili, O. C.; Porcionatto, M. A.; Nader, H. B.; Dietrich, C. P.; Brentani, R. R.; Gremski, W. (Kluwer Academic Publ, 2000-07-01)
      Loxoscelism or necrotic arachnidism are terms used to describe lesions and reactions induced by bites (envenomation) from spiders of the genus Loxosceles. Envenomation has been reported to provoke dermonecrosis and haemorrhage ...
    • Fucan inhibits Chinese hamster ovary cell (CHO) adhesion to fibronectin by binding to the extracellular matrix 

      Rocha, HAO; Franco, CRC; Trindade, E. S.; Veiga, S. S.; Leite, E. L.; Nader, H. B.; Dietrich, C. P. (Georg Thieme Verlag Kg, 2005-07-01)
      In recent years, sulfated fucans have emerged as an important class of natural biopolymers. in this study, the anti-adhesive activity of a fucan from the brown seaweed Spatoglossum schroederi was analyzed using tumorigenic ...
    • Identification and partial characterisation of hyaluronidases in Lonomia obliqua venom 

      Gouveia, AID; Silveira, R. B. da; Nader, H. B.; Dietrich, C. P.; Gremski, W.; Veiga, S. S. (Elsevier B.V., 2005-03-15)
      By studying Lonomia obliqua (caterpillar) venom we were able to detect a lytic activity on purified hyaluronic acid. the venom hydrolyses purified chondroitin sulphate, but was unable to degrade either heparan sulphate or ...
    • Identification of proteases in the extract of venom glands from brown spiders 

      Silveira, R. B. da; Santos, J. F. dos; Mangili, O. C.; Veiga, S. S.; Gremski, W.; Nader, H. B.; von Dietrich, C. P. (Elsevier B.V., 2002-06-01)
      In the present investigation, in order to dispute the rational criticism against the presence of proteolytic enzymes in the electrostimulated venom obtained from spiders of the genus Loxosceles, as a consequence of ...
    • In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells 

      Veiga, S. S.; Zanetti, V. C.; Franco, CRC; Trindade, E. S.; Porcionatto, M. A.; Mangili, O. C.; Gremski, W.; Dietrich, C. P.; Nader, H. B. (Elsevier B.V., 2001-05-01)
      The effect of brown spider (Loxosceles intermedia) venom on endothelial cells was investigated in vivo and in vitro. Morphological and ultrastructural observations by light microscopy and transmission electron microscopy ...
    • Isolation and biochemical characterization of a fibrinolytic proteinase from Bothrops leucurus (white-tailed jararaca) snake venom 

      Bello, C. A.; Hermogenes, ALN; Magalhaes, A.; Veiga, S. S.; Gremski, L. H.; Richardson, M.; Sanchez, E. F. (Elsevier B.V., 2006-02-01)
      In investigations aimed at characterizing snake venom clot-dissolving enzymes, we have purified a fibrinolytic proteinase from the venom of Bothrops leucurus (white-tailed-jararaca). the proteinase was purified to homogeneity ...
    • Nephrotoxicity caused by brown spider venom phospholipase-D (dermonecrotic toxin) depends on catalytic activity 

      Kusma, J.; Chaim, O. M. [UNIFESP]; Wille, A. C. M.; Ferrer, V. P.; Sade, Y. B.; Donatti, L.; Gremski, W.; Mangili, O. C.; Veiga, S. S. (Elsevier B.V., 2008-11-01)
      Bites from brown spiders (Loxosceles genus) have clinical manifestations including skin necrosis with gravitational spreading, and systemic involvement that may include renal failure, hemolysis, and thrombocytopenia. Mice ...
    • Oligosaccharide residues of Loxosceles intermedia (brown spider) venom proteins: dependence on glycosylation for dermonecrotic activity 

      Veiga, S. S.; Gremski, W.; Santos, VLP dos; Feitosa, L.; Mangili, O. C.; Nader, H. B.; Dietrich, C. P.; Brentani, R. R. (Elsevier B.V., 1999-04-01)
      Loxosceles spp. (brown spider) envenomation has been reported to provoke dermonecrosis and haemorrhage at the bite site (a hallmark of accidents) and, to a lesser extent, thrombocytopenia, hemolysis and disseminated ...
    • Presence of a laminin-binding chondroitin sulfate proteoglycan at the cell surface of a human melanoma cell Mel-85 

      Elias, MCQB; Veiga, S. S.; Gremski, W.; Porcionatto, M. A.; Nader, H. B.; Brentani, R. R. (Kluwer Academic Publ, 1999-07-01)
      Working with Mel-85 (a human melanoma cell line), we have been able to detect a laminin-binding molecule with an apparent molecular mass of 100/110 kDa (Mel-85-LBM). Reduction with beta-mercaptoethanol decreases its molecular ...
    • Structural and ultrastructural description of the venom gland of Loxosceles intermedia (brown spider) 

      Santos, VLP dos; Franco, CRC; Viggiano, RLL; Silveira, R. B. da; Cantao, M. P.; Mangili, O. C.; Veiga, S. S.; Gremski, W. (Elsevier B.V., 2000-02-01)
      The brown spider, genus Loxosceles, is becoming of great medical importance, with envenomation (Loxoscelism) occurring throughout the world. the biological activities of the brown spider venom usually include dermonecrotic ...