Now showing items 1-15 of 15

    • 2,3-Di-O-sulfo glucuronic acid: An unmodified and unusual residue in a highly sulfated chondroitin sulfate from Litopenaeus vannamei 

      Cavalcante, Romulo S.; Brito, Adriana S.; Palhares, Lais C. G. F.; Lima, Marcelo A. [UNIFESP]; Cavalheiro, Renan P. [UNIFESP]; Nader, Helena B. [UNIFESP]; Sassaki, Guilherme L.; Chavante, Suely F. (Elsevier Sci Ltd, 2018)
      The occurrence of a natural and unmodified highly sulfated chondroitin sulfate from Litopenaeus vannamei heads (sCS) is herein reported. Its partial digestion by Chondroitinases AC and ABC together with its electrophoretic ...
    • Antithrombin stabilisation by sulfated carbohydrates correlates with anticoagulant activity 

      Lima, Marcelo A. [UNIFESP]; Hughes, Ashley J.; Veraldi, Noemi; Rudd, Timothy R.; Hussain, Rohanah; Brito, Adriana S. [UNIFESP]; Chavante, Suely F.; Tersariol, Ivarne I. [UNIFESP]; Siligardi, Giuliano; Nader, Helena B. [UNIFESP]; Yates, Edwin A. (Royal Soc Chemistry, 2013-05-01)
      Thermal stabilisation of native antithrombin-III (AT), determined using differential scanning fluorimetry, correlated with the anticoagulant activity of heparin and heparin-related saccharides. Similar conformational changes ...
    • Chemical reduction of carboxyl groups in heparin abolishes its vasodilatory activity 

      Paredes-Gamero, Edgar J. [UNIFESP]; Medeiros, Valquiria P. [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Accardo, Camila de Melo [UNIFESP]; Farias, Eduardo H. C. [UNIFESP]; Sassaki, Guilherme I.; Campana, Patricia T.; Miranda, Antonio [UNIFESP]; Ferreira, Alice T. [UNIFESP]; Tersariol, Ivarne L. S. [UNIFESP]; Nader, Helena Bonciani [UNIFESP] (Wiley-Blackwell, 2012-04-01)
      Previous studies have shown that heparin induces vascular relaxation via integrin-dependent nitric oxide (NO)-mediated activation of the muscarinic receptor. the aim of this study was to identify the structural features ...
    • Enhanced Tumorigenic Potential of Colorectal Cancer Cells by Extracellular Sulfatases 

      Vicente, Carolina M. [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Yates, Edwin A. [UNIFESP]; Nader, Helena B. [UNIFESP]; Toma, Leny [UNIFESP] (Amer Assoc Cancer Research, 2015-03-01)
      Heparan sulfate endosulfatase-1 and -2 (SULF1 and SULF2) are two important extracellular 6-O-endosulfatases that remove 6-O sulfate groups of N-glucosamine along heparan sulfate (HS) proteoglycan chains often found in the ...
    • F-19 labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy 

      Lima, Marcelo A. [UNIFESP]; Cavalheiro, Renan P. [UNIFESP]; Viana, Gustavo M. [UNIFESP]; Meneghetti, Maria C. Z. [UNIFESP]; Rudd, Timothy R.; Skidmore, Mark A.; Powell, Andrew K.; Yates, Edwin A. [UNIFESP] (Springer, 2017)
      Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues ...
    • Heparin prevents Zika virus induced-cytopathic effects in human neural progenitor cells 

      Ghezzi, Silvia; Cooper, Lynsay; Rubio, Alicia; Pagani, Isabel; Capobianchi, Maria Rosaria; Ippolito, Giuseppe; Pelletier, Julien; Meneghetti, Maria Cecilia Z. [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Skidmore, Mark A.; Broccoli, Vania; Yates, Edwin A. [UNIFESP]; Vicenzi, Elisa (Elsevier Science Bv, 2017)
      The recent Zika virus (ZIKV) outbreak, which mainly affected Brazil and neighbouring states, demonstrated the paucity of information concerning the epidemiology of several flaviruses, but also highlighted the lack of ...
    • High-sensitivity visualisation of contaminants in heparin samples by spectral filtering of H-1 NMR spectra 

      Rudd, Timothy R.; Gaudesi, Davide; Lima, Marcelo A. [UNIFESP]; Skidmore, Mark A.; Mulloy, Barbara; Torri, Giangiacomo; Nader, Helena B. [UNIFESP]; Guerrini, Marco; Yates, Edwin A. (Royal Soc Chemistry, 2011-01-01)
      A novel application of two-dimensional correlation analysis has been employed to filter H-1 NMR heparin spectra distinguishing acceptable natural variation and the presence of foreign species. Analysis of contaminated ...
    • Insights into the N-Sulfation Mechanism: Molecular Dynamics Simulations of the N-Sulfotransferase Domain of Ndst1 and Mutants 

      Gesteira, Tarsis F. [UNIFESP]; Pol-Fachin, Laercio; Coulson-Thomas, Vivien Jane [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Verli, Hugo; Nader, Helena Bonciani [UNIFESP] (Public Library Science, 2013-08-05)
      Sulfation patterns along glycosaminoglycan (GAG) chains dictate their functional role. the N-deacetylase N-sulfotransferase family (NDST) catalyzes the initial downstream modification of heparan sulfate and heparin chains ...
    • Insights into the role of 3-O-sulfotransferase in heparan sulfate biosynthesis 

      Zorel Meneghetti, Maria Cecilia [UNIFESP]; Ferreira, Tarsis Gesteira; Tashima, Alexandre Keiji [UNIFESP]; Chavante, Suely F.; Yates, Edwin Alexander [UNIFESP]; Liu, Jian; Nader, Helena Bonciani [UNIFESP]; Lima, Marcelo A. [UNIFESP] (Royal Soc Chemistry, 2017)
      3-O-Sulfotransferase enzyme (sHS) from Litopenaeus vannamei was cloned and its substrate specificity was investigated against a number of GAG structures, including modified heparin polysaccharides and model oligosaccharides. ...
    • A New Approach for Heparin Standardization: Combination of Scanning UV Spectroscopy, Nuclear Magnetic Resonance and Principal Component Analysis 

      Lima, Marcelo A. [UNIFESP]; Rudd, Timothy R.; Farias, Eduardo H. C. de [UNIFESP]; Ebner, Lyvia F. [UNIFESP]; Gesteira, Tarsis F. [UNIFESP]; Souza, Lauro M. de; Mendes, Aline [UNIFESP]; Córdula, Carolina Ribeiro [UNIFESP]; Martins, Joao R. M. [UNIFESP]; Hoppensteadt, Debra; Fareed, Jawed; Sassaki, Guilherme L.; Yates, Edwin A.; Tersariol, Ivarne Luis dos Santos [UNIFESP]; Nader, Helena Bonciani [UNIFESP] (Public Library Science, 2011-01-18)
      The year 2007 was marked by widespread adverse clinical responses to heparin use, leading to a global recall of potentially affected heparin batches in 2008. Several analytical methods have since been developed to detect ...
    • On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+ 

      Córdula, Carolina Ribeiro [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Shinjo, Samuel K. [UNIFESP]; Gesteira, Tarsis F. [UNIFESP]; Pol-Fachin, Laercio; Coulson-Thomas, Vivien Jane [UNIFESP]; Verli, Hugo; Yates, Edwin A. [UNIFESP]; Rudd, Timothy R.; Pinhal, Maria A. S. [UNIFESP]; Toma, Leny [UNIFESP]; Dietrich, Carl P. [UNIFESP]; Nader, Helena B. [UNIFESP]; Tersariol, Ivarne L. S. [UNIFESP] (Royal Soc Chemistry, 2014-01-01)
      The structurally diverse polysaccharide lyase enzymes are distributed from plants to animals but share common catalytic mechanisms. One, heparinase I (F. heparinum), is employed in the production of the major anticoagulant ...
    • A robust method to quantify low molecular weight contaminants in heparin: detection of tris(2-n-butoxyethyl) phosphate 

      Sassaki, Guilherme L.; Riter, Daniel S.; Santana Filho, Arquimedes P.; Guerrini, Marco; Lima, Marcelo A. [UNIFESP]; Cosentino, Cesare; Souza, Lauro M.; Cipriani, Thales R.; Rudd, Timothy R.; Nader, Helena B. [UNIFESP]; Yates, Edwin A.; Gorin, Philip A. J.; Torri, Giangiacomo; Iacomini, Marcello (Royal Soc Chemistry, 2011-01-01)
      Recently, oversulfated chondroitin sulfate (OSCS) was identified in contaminated heparin preparations, which were linked to several adverse clinical events and deaths. Orthogonal analytical techniques, namely nuclear ...
    • Structural and Pharmacological Profile of Generic Enoxaparins Used in Brazil 

      Lima, Marcelo A. [UNIFESP]; Farias, Eduardo H. C. de [UNIFESP]; Gray, Angel; Sadeghi, Nasir; Gesteira, Tarsis F. [UNIFESP]; Cavalheiro, Renan P. [UNIFESP]; Hoppensteadt, Debra; Fareed, Jawed; Sassaki, Guilherme L.; Nader, Helena B. [UNIFESP] (Sage Publications Inc, 2012-07-01)
      Generic active pharmaceutical ingredients (APIs) have been commonly used in Brazil, since 1999, but most of them are synthetic and small molecules. Recently, a large number of generic enoxaparins were introduced into the ...
    • SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells 

      Vicente, Carolina M. [UNIFESP]; Lima, Marcelo A. [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Toma, Leny [UNIFESP] (Biomed Central Ltd, 2015-03-14)
      Background: SULF2 is a 6-O-endosulfatase which removes 6-O sulfate residues from N-glucosamine present on heparan sulfate (HS). the sulfation pattern of HS influences signaling events mediated by heparan sulfate proteoglycans ...
    • Ultra-low-molecular-weight heparins: Precise structural features impacting specific anticoagulant activities 

      Lima, Marcelo A. [UNIFESP]; Viskov, Christian; Herman, Frederic; Gray, Angel L.; Farias, Eduardo H. C. de [UNIFESP]; Cavalheiro, Renan P. [UNIFESP]; Sassaki, Guilherme L.; Hoppensteadt, Debra; Fareed, Jawed; Nader, Helena B. [UNIFESP] (Schattauer Gmbh-verlag Medizin Naturwissenschaften, 2013-03-01)
      Ultra-low-molecular-weight heparins (ULMWHs) with better efficacy and safety ratios are under development; however, there are few structural data available. the main structural features and molecular weight of ULMWHs were ...