Navegando por Palavras-chave "virulence properties"

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    An enteroaggregative Escherichia coli strain of serotype O111 : H12 damages and invades cultured T84 cells and human colonic mucosa
    (Elsevier B.V., 2001-09-25) Abe, Cecilia Mari [UNIFESP]; Knutton, Stuart; Pedroso, Margareth Z. [UNIFESP]; Freymuller, Edna [UNIFESP]; Gomes, Tania Aparecida Tardelli [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Birmingham
    The pathogenic mechanisms of enteroaggregative Escherichia coh (EAEC) are not well defined. We investigated the interaction of EAEC strain 236 (serotype O111:H12) with polarised Caco-2 and T84 human intestinal epithelia] cells lines, and,vith human jejunal and colonic mucosa. Strain 236 adhered to both polarised cell lines and to both intestinal tissue types, but caused severe damage and was invasive only in T84 cells and colonic mucosa. in contrast, prototype EAEC strain 042, which also adhered to the Cultured intestinal cell lines, did not adhere to or invade jejunal or colonic tissue. These observations suggest a heterogeneity of virulence properties within the EAEC category of diarrhoea-causing E. coli. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science BN. All rights reserved.
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    Virulence properties of Escherichia coli O111:H12 strains
    (Wiley-Blackwell, 1997-01-01) Monteiro-Neto, Valério; Campos, Leila Carvalho; Ferreira, Antonio José Piantino; Gomes, Tania Aparecida Tardelli [UNIFESP]; Trabulsi, Luiz Rachid; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Seventeen Escherichia coli O111:H12 strains isolated from the feces of children with acute diarrhea were studied with regard to their adherence properties and other virulence characteristics. All strains showed an aggregative adherence pattern to HEp-2 cells and agglutinated bovine and sheep red cells in the presence of mannose. These strains did not have gene sequences homologous to the aggregative adherence fimbria I gene and did not react with any of the DNA probes used to detect other virulence genes in enteropathogens. With one exception, the O111:H12 strains did not induce fluid accumulation in the rabbit ileal loop assay, although 16 of the strains had the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) gene sequences. A 60-70 MDa plasmid was present in 16 of the strains studied. We conclude that the O111 :H12 serotype, one of the first E. coli identified in infantile diarrhea, belongs to the enteroaggregative E. coli category but the genes encoding its adherence phenotype are distinct from those previously described.