Navegando por Palavras-chave "transposons"

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    Frequent Tn2 Misannotation in the Genetic Background of rmtB
    (Amer Soc Microbiology, 2017) Martins, Willames M. B. S. [UNIFESP]; Gales, Ana C. [UNIFESP]
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    Transposable elements and two other molecular markers as typing tools for the genus Paracoccidioides
    (Oxford Univ Press, 2015-02-01) Alves, Fernanda Lourenco; Ribeiro, Mariceli Araujo; Hahn, Rosane Christine; Teixeira, Marcus de Melo; Camargo, Zoilo Pires de [UNIFESP]; Cisalpino, Patricia Silva; Marini, Marjorie Mendes [UNIFESP]; Universidade Federal de Minas Gerais (UFMG); Univ Fed Espirito Santo; Univ Fed Mato Grosso; Universidade de Brasília (UnB); Universidade Federal de São Paulo (UNIFESP)
    Studies comparing Paracoccidioides brasiliensis and Paracoccidioides lutzii have shown that these fungi have significant genomic differences that may have implications in the clinical manifestation, diagnosis, and treatment of paracoccidioidomycosis caused by them. Thus, molecular typing methods are required that can distinguish between various species of Paracoccidioides. the aim of this study was to explore the potential use as molecular markers of the transposable elements Trem A-H recently identified and characterized in the genus Paracoccidioides as a means of differentiating the species. We take advantage of the abundance and distribution of these transposons in the Paracoccidioides genomes to develop a simple and highly reproducible polymerase chain reaction (PCR)-based technique. Furthermore we compare the performance of this test with two other molecular markers already in use to identify these fungi.