Navegando por Palavras-chave "synovial fluid"
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- ItemAcesso aberto (Open Access)Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints(Korean Soc Veterinary Science, 2015-03-01) Moreira, Juliana Junqueira [UNIFESP]; Moraes, Ana Paula Lopes de [UNIFESP]; Brossi, Patricia Monaco [UNIFESP]; Machado, Thais Sodré de Lima [UNIFESP]; Michelacci, Yara Maria [UNIFESP]; Massoco, Cristina de Oliveira; Baccarin, Raquel Yvonne Arantes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)This experimental controlled study was performed to evaluate the composition of autologous processed plasma (APP), and the effects of APP intra-articular injection into healthy equine metacarpophalangeal joints. the effects on joints were analysed with a short-phase protocol and a prolonged-phase protocol using saline-injected joints as controls. for the short protocol, horses received one intra-articular APP injection. Synovial fluid samples were collected prior to the injection and 3, 6, 24, 48, and 16 h after treatment. for the prolonged protocol, the joints received three weekly injections of APP, and samples were collected at 0, 7, 14, 21, and 28 days before APP administration. IL1-ra level was found to be increased in APP compared to plasma. Upon intra-articular administration of APP, transient (up to 24 h) increases in white blood cell (WBC) counts along with elevated protein and prostaglandin E-2 (PGE(2)) concentrations were observed in the treated joints. Over the 28-day observation period, APP did not elicit changes relative to baseline levels, but WBC counts, PGE(2) and chondroitin sulphate concentrations were lower than those found in the control. in conclusion, APP intra-articular injection induced a mild and transitory inflammatory response but no inflammation reaction was observed over a longer period of treatment and observation.
- ItemAcesso aberto (Open Access)Glycosaminoglycan loss from cartilage after anterior cruciate ligament rupture: influence of time since rupture and chondral injury(Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia, 2008-02-01) Mattiello, Stela Márcia; Cintra Neto, Paulo Felix de Araujo; Lima, Geovane Elias Guidini de; Pinto, Karina Nogueira Zambone; Cohen, Moises [UNIFESP]; Pimentel, Edson Rosa; Universidade Federal de São Carlos Department of Physiotherapy; Universidade Federal de São Carlos Department of Morphology and Pathology; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)OBJECTIVE: To quantify the concentration of sulfated glycosaminoglycans (GAGs) concentration in the synovial fluid (SF) of knees with chronic anterior cruciate ligament (ACL) rupture and to identify possible associations between GAG concentration in SF and the time elapsed since rupture and degree of chondral injury. METHOD: Fourteen adult male subjects with total unilateral ACL rupture, which had occurred between 5 and 144 months earlier, were assessed. All subjects underwent joint aspiration; it was possible to collect SF from ten individuals. The samples were quantified to determine the GAG concentration using dimethylmethylene blue (DMMB) staining. The degree of chondral injury was macroscopically evaluated using the modified Mankin histological scale. Spearman correlation test (< 0.05) was used to evaluate the association between GAG concentration and chondral injury, and Pearson correlation test (< 0.05) was used to evaluate the association between GAG concentration and the time elapsed since rupture. RESULTS: The GAG concentration in SF showed a mean variation of 73.84 ± 40.75 µg/ml, with a mean time of 40.4 ± 40.3 months since the rupture. There was no correlation between GAG concentration and time since the rupture (r= -0.09, p= 0.81). The chondral injury grades found were 0, 1, 4 and 5. There was no correlation between chondral injury grade and GAG concentration in SF (r= -0.41, p= 0.24). CONCLUSION: After at least 5 months, the GAG concentration in SF from knees with ACL rupture is independent of the time elapsed since rupture and/or the severity of chondral injury.
- ItemSomente MetadadadosInterleukin 18 as a marker of disease activity and severity in patients with juvenile idiopathic arthritis(J Rheumatol Publ Co, 2007-04-01) Lotito, Ana Paola N.; Campa, Ana; Silva, Clovis A. A.; Kiss, Maria H. B.; Mello, Suzana B. V.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Objective. To verify the importance of interleukin 18 (IL-18) in the pathogenesis of juvenile idiopathic arthritis (JIA). We measured IL-18 levels in synovial fluid (SF) and serum, and determined their correlation with measures of disease activity and severity.Methods. Fifty patients with JIA (13 systemic, 13 polyarticular, 24 oligoarticular) and 25 matched controls were analyzed. Cytokire levels (IL-1 beta, IL-1Ra, IL-6, and IL-18) were quantified in serum and SF by ELISA, and disease activity measures were evaluated immediately after knee articular puncture. Radiological assessment was made according to the Steinbrocker method. Statistical analysis was performed by Spearman's rank-order correlation and Mann-Whitney rank test.Results. All the analyzed cytokine levels (IL-1, IL-IRa, IL-6, and IL-18) were higher in patients' sera than in controls. Remarkably, in patients with JIA, IL-18 SF levels did not differ from those of serum; they were positively correlated. The levels of IL-18 (SF and serum) were positively correlated with measures of disease activity: C-reactive protein, number of active joints, and radiological score, as well as with levels of IL-1, IL-1Ra, and IL-6. Moreover, IL-18 and IL-6 levels in SF and serum were much higher in patients with systemic disease compared to the other types of disease onset. In contrast, IL-1 and IL-1Ra were not different among JIA subtypes.Conclusion. Our results strongly suggest the participation of IL-18 in the pathophysiology of JIA. The positive correlation of this cytokine with several measures of articular inflammation and disease severity suggests that IL-18 could be a better target for the treatment of arthritis.
- ItemSomente MetadadadosSynovial fluid chondroitin sulphate indicates abnormal joint metabolism in asymptomatic osteochondritic horses(Wiley-Blackwell, 2012-07-01) Machado, T. S. L.; Correia da Silva, L. C. L.; Baccarin, Raquel Yvonne Arantes [UNIFESP]; Michelacci, Y. M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Reasons for performing study: Alternative methods to evaluate the joint condition in asymptomatic osteochondrosis dissecans (OCD) and other joint diseases may be useful. Objectives: To investigate possible changes in synovial fluid composition that may lead to joint conditions in asymptomatic OCD, in mature horses. Methods: Animals aged >2 years, of different breeds, with OCD in the intermediate ridge of distal tibia, symptomatic or not, were studied. Synovial fluid samples (10 healthy; 11 asymptomatic OCD; 25 symptomatic OCD) were collected by arthroscopy from 29 horses. Glycosaminoglycans (GAGs) were analysed by a combination of agarose gel electrophoresis and enzymatic degradation with specific GAG lyases. the viscosity, white blood cell (WBC) count, protein concentration and hyaluronic acid (HA) molecular weight were also determined. Results: the method used here to analyse synovial fluid GAGs is reliable, reproducible and specific. the main synovial fluid GAGs are HA and chondroitin sulphate (CS), 93% and 7% respectively in normal horses. in symptomatic OCD, the concentrations of both increased (expressed as GAG/urea ratios), but CS increased more. the CS increased also in asymptomatic OCD. An inflammatory reaction was suggested by the increased WBC counts in OCD. the molecular weight of the synovial fluid HA was reduced in OCD, explaining the lower viscosity observed. Conclusions: the increased CS in synovial fluid of OCD joints in mature horses suggests that the synovial fluid CS and the WBC count are good markers of the joint conditions, allowing the identification of pathological phase in joint diseases. Potential relevance: the analysis of synovial fluid GAGs shows that cartilage damage occurs even in asymptomatic OCD, implying that arthroscopic removal of osteochondral fragments should be performed even in asymptomatic OCD.