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- ItemSomente MetadadadosAnálise dos aspectos funcionais dos espermatozoides e do perfil proteômico de plasma seminal de homens tabagistas(Universidade Federal de São Paulo (UNIFESP), 2014-02-12) Antoniassi, Mariana Pereira [UNIFESP]; Bertolla, Ricardo Pimenta Bertolla [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objectives: To evaluate the effect of smoking on sperm functional aspects, semen oxidative stress and seminal plasma proteomic profile. Method: A cross-sectional observational study including 20 nonsmokers patients with normal semen quality, according to the World Health Organization (2010) (control group), and 20 smoking patients (study group). Beyond semen analysis, sperm function tests were performed on each patient: evaluation of DNA integrity by alkaline Comet assay; analysis of mitochondrial activity using 3,3' - diaminobenzidine (DAB) staining; and evaluation of acrosomal integrity by PNA (Peannut Agglutinin) binding to the outer acrosomal membrane. The seminal plasma was used to assess the level of oxidative stress by TBARS (Thiobarbituric acid reactive substances) quantification and label-free quantitative proteomic analysis by LC-MS/MS. Groups were compared using a Student?s T test (p <0.05), considering a minimum fold-change of 2.0 (or maximum of 0.5). Results: The group of smokers presented higher percentage of DNA damage (Comet class III and IV), partially and fully inactive mitochondria (DAB class III and IV) and non-intact acrosomes when compared to the control group. No difference in the level of oxidative stress among groups was observed. With respect to proteomic analysis, 422 proteins were identified and quantified, of which one protein was absent (Mammaglobin-B), 6 proteins were underexpressed (Prolargin, Poly(rC)- binding protein 1, Bile salt-activated lipase, Carbonyl reductase [NADPH] 1, Phospholipase A2, membrane associated, CD44 antigen) and 3 proteins were overexpressed among smokers (S100-A9, Neutrophil gelatinase-associated lipocalin e Alpha-1-acid glycoprotein 1). Conclusions: Cigarette smoking alters the functional quality of sperm and the seminal plasma proteomic profile of men, but does not alter the levels of oxidative stress. The overexpressed proteins have functions related to the metabolism of steroids and derivatives of arachidonic acid, as well as preservation of the extracellular scaffold. Among the overexpressed proteins, we highlight the immune function.
- ItemSomente MetadadadosAssociation between the seminal plasma proteome and sperm functional traits(Elsevier Science Inc, 2016) Intasqui, Paula [UNIFESP]; Camargo, Mariana [UNIFESP]; Antoniassi, Mariana Pereira [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Carvalho, Valdemir Melechco; Morais Cardozo, Karina Helena; Zylbersztejn, Daniel Suslik [UNIFESP]; Bertolla, Ricardo Pimenta [UNIFESP]Objective: To analyze the seminal plasma proteome and biological functions associated with sperm functional alterations. Design: Cross-sectional study. Setting: University andrology and research laboratories. Patient(s): A total of 156 normozoospermic men. Intervention(s): Sperm mitochondrial activity, acrosome integrity, and DNA fragmentation were evaluated in a semen aliquot. Remaining semen was centrifuged, and seminal plasma was utilized for proteomic analysis (liquid chromatography-tandem mass spectrometry). Patients were divided into percentiles (15%) to form the following groups: substudy 1, high (control, n = 26) and low (study, n = 23) sperm mitochondrial activity; substudy 2, high (control, n = 23) and low (study, n = 22) sperm acrosome integrity; and substudy 3, low (control, n = 22) and high (study, n = 22) sperm DNA fragmentation. Groups were compared using univariate and multivariate analyses. Differentially expressed proteins were used for functional enrichment analysis. Main Outcome Measure(s): Seminal plasma proteome and postgenomic pathways are associated with several sperm functional traits. Result(s): In total, 506, 493, and 464 proteins were observed in substudies 1, 2, and 3, respectively. Enriched functions in substudy 1 were intramolecular oxidoreductase activity, aminoglycans catabolism, endopeptidases inhibition, lysosomes, and acute-phase response (study group). In substudy 2, main enriched functions were phospholipase inhibition, arachidonic acid metabolism, exocytosis, regulation of acute inflammation, response to hydrogen peroxide, and lysosomal transport (study group). In substudy 3, enriched functions were prostaglandin biosynthesis and fatty acid binding (study group). We proposed eight, six, and eight seminal biomarkers for substudies 1, 2, and 3, respectively. Conclusion(s): Seminal plasma proteome reflects sperm mitochondrial activity reduction, acrosome damage, and DNA fragmentation, with several postgenomic functions related to these alterations. (C) 2016 by American Society for Reproductive Medicine.
- ItemAcesso aberto (Open Access)Effects of acupuncture and moxa treatment in patients with semen abnormalities(Science China Press, 2003-12-01) Gurfinkel, Edson [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Yamamura, Ysao [UNIFESP]; Srougi, Miguel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Aim: To evaluate the effect of Chinese Traditional Medicine, acupuncture and moxa treatment, on the semen quality in patients with semen abnormalities. Methods: In a prospective, controlled and blind study, nineteen patients, aged 24 years similar to 42 years and married for 3 years similar to 11 years without children with semen abnormalities in concentration, morphology and/or progressive motility without apparent cause, were randomized into two groups and submitted to acupuncture and moxa treatment at the therapeutic (Study Group) and the indifferent points (Control Group), respectively, for 10 weeks. Semen analyses were performed before and after the treatment course. Results: The patients of the Study Group presented a significant increase in the percentage of normal-form sperm compared to the Control Group (calculated U = 16.0, critical U = 17.0). Conclusion: The Chinese Traditional Medicine acupuncture and moxa techniques significantly increase the percentage of normal-form sperm in infertile patients with oligoastenoteratozoospermia without apparent cause. (Asian J Androl 2003 Dec; 5: 345-348).
- ItemSomente MetadadadosGonadal function in male adolescents and young males with juvenile onset systemic lupus erythematosus(J Rheumatol Publ Co, 2002-09-01) Silva, Clovis AA; Hallak, Jorge; Pasqualotto, Fabio F.; Barba, Mario F.; Saito, Maria I.; Kiss, Maria HB; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Objective. To evaluate gonadal function in male adolescents and young men with juvenile onset systemic lupus erythematosus (SLE).Methods. Four young men with SLE underwent clinical and laboratory evaluation, testicular ultrasound, follicle stimulating hormone, luteinizing hormone, prolactin, testosterone, and and-sperm antibody determination. The semen analyses were per-formed according to the WHO guidelines and Kruger strict criteria. All patients were asked to provide 3 semen samples over a period of 2 months. A new sample was collected 6 months later.Results. The median disease duration was 6.6 years. The median age at initial ejaculation was 13.5 years. All 4 patients had severe disease with renal involvement (WHO class IV or V). The SLICC/ACR damage index at the time of study entry ranged between 0 and 3. The patients' Tanner stage was P5G5; all reported normal erection and libido. Gonadal evaluation by thorough examination of the genitalia and ultrasound was normal. Anti-sperm antibodies were negative in all patients. Only one patient showed high FSH and LH levels. The initial and final semen evaluations of the 4 patients were abnormal (azoospermia, oligoastenoteratospermia, or teratospermia). One patient was receiving azathioprine and 2 were receiving cyclophosphamide at the time of study entry.Conclusion. Although these patients had normal sexual activity and normal external genitalia, their fertility was decreased based on the sperm abnormalities. Serial semen analyses in larger study populations will be necessary to clarify the degree and duration of sperm abnormalities in male patients with SLE in general.
- ItemSomente MetadadadosIndividual and seminal characteristics of patients with testicular germ cell tumors(Elsevier B.V., 2010-11-01) Fraietta, Renato [UNIFESP]; Spaine, Deborah Montagnini [UNIFESP]; Bertolla, Ricardo Pimenta [UNIFESP]; Ortiz, Valdemar [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To analyze the characteristics of patients with testicular germ cell cancer and compare patients' sperm quality according to histologic type (seminomatous and nonseminomatous tumors).Design: Prospective study.Setting: Sperm bank at a university.Patient(s): One hundred consecutive patients with testicular tumor who had been referred to our infertility center for cryopreservation, between 2004 and 2006.Intervention(s): A questionnaire, through personal interview, was given to all patients and collection of seminal data before cryopreservation was performed.Main Outcome Measure(s): Patient characteristics, including age, time between diagnosis and orchiectomy, history of cryptorchidism, histologic type, and seminal analysis were taken into consideration.Result(s): the mean age of the patients at the time of diagnosis was 26.9 years. the mean time between cancer suspicion and the diagnosis of neoplasm was 58.9 days, and 19.4 more days were necessary until orchiectomy was performed. Eleven patients had a history of cryptorchidism. Thirty-seven patients had seminomatous tumors. Men with a seminoma present a higher number of motile and morphologically normal sperm in the ejaculate than men with a nonseminoma, although individual semen variables are not different.Conclusion(s): the majority of the patients with testicular cancer, referred to our infertility center, are very young, single, do not have children, and are unaware of their fertility potential status by the time diagnosis is made. Men with a nonseminoma present semen of lower quality. (Fertil Steril (R) 2010; 94: 2107-12. (C) 2010 by American Society for Reproductive Medicine.)
- ItemAcesso aberto (Open Access)Male infertility in spinal cord trauma(Sociedade Brasileira de Urologia, 2005-08-01) Utida, Cristiano [UNIFESP]; Truzzi, Jose C. [UNIFESP]; Bruschini, Homero [UNIFESP]; Simonetti, Rogerio [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Srougi, Miguel [UNIFESP]; Ortiz, Valdemar [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); San Francisco Home School Section of UrologyEvery year there are 10 thousand new cases of patients victimized by spinal cord trauma (SCT) in the United States and it is estimated that there are 7 thousand new cases in Brazil. Eighty percent of patients are fertile males. Infertility in this patient group is due to 3 main factors resulting from spinal cord lesions: erectile dysfunction, ejaculatory disorder and low sperm counts. Erectile dysfunction has been successfully treated with oral and injectable medications, use of vacuum devices and penile prosthesis implants. The technological improvement in penile vibratory stimulation devices (PVS) and rectal probe electro-ejaculation (RPE) has made such procedures safer and accessible to patients with ejaculatory dysfunction. Despite the normal number of spermatozoa found in semen of spinal cord-injured patients, their motility is abnormal. This change does not seem to be related to changes in scrotal thermal regulation, frequency of ejaculation or duration of spinal cord damage but to factors related to the seminal plasma. Despite the poor seminal quality, increasingly more men with SCT have become fathers through techniques ranging from simple homologous insemination to sophisticated assisted reproduction techniques such as intracytoplasmic sperm injection (ICSI).
- ItemSomente MetadadadosSexual behavior and recurrent spontaneous abortion(Elsevier B.V., 2005-02-01) Mattar, Rosiane [UNIFESP]; Soares, RVP; Daher, S.; Universidade Federal de São Paulo (UNIFESP)