Navegando por Palavras-chave "perfusion"

Agora exibindo 1 - 3 de 3
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Effects of eurocollins solution as aortic flush for the procurement of human pancreas
    (Lippincott Williams & Wilkins, 2005-11-15) Gonzalez, A. M.; Filho, GJL; Pestana, JOM; Linhares, M. M.; Silva, MHG; Moura, RMAM; Melaragno, C.; Sa, JR de; Rangel, E. B.; Trivino, T.; Universidade Federal de São Paulo (UNIFESP)
    Background. Belzer solution is considered to be the best preservation media used for pancreas transplantation; however, its high cost accounts for approximately 14.5% of all resources allocated by the Brazilian government toward each pancreatic transplant. the objective of the present study was to test a reduction of Belzer solution during pancreas harvest, thereby lowering procedural cost.Methods. the patients received pancreas-kidney transplantations during the period from January 2003 to August 2004. Patients were divided into two groups. Patients assigned to Group A (n =30) received only Belzer solution (2 L through the aorta artery), whereas patients in Group B (n = 16) were perfused first with I L of Eurocollins solution followed by I L of Belzer solution. the two groups were assessed for differences in the following clinical parameters: the need for insulin replacement or antifungal and anticytomegalovirus treatment, pancreatitis, acute cellular rejection, graft vascular thrombosis, fistulas, intra-abdominal collection, graft loss, deaths, pancreatic ischemia time, and average hospitalization time.Results. No statistically significant differences were observed in any of the parameters analyzed (P < 0.05). the use of Eurocollins solution, followed by Belzer solution during pancreas harvesting, did not result in differences in graft survival or functionality, postsurgical complications, or patient survival and hospitalization time, when compared to the use of Belzer solution alone.Conclusions. Perfusion with 1 L of Eurocollins solution followed by I L of Belzer solution during pancreas harvesting seems to be a simple and efficient alternative for reducing the costs of the harvesting process.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Effects of transcutaneous electrical nerve stimulation on fetal and placental development in an experimental model of placental insufficiency
    (Taylor & Francis Ltd, 2016) Guimaraes, Camila S. O.; Gomes, Bruno B. F.; Oliveira, Rafael A.; Yamamoto, Leandro R.; Rocha, Laura P.; Gloria, Maria A. [UNIFESP]; Machado, Juliana R.; Camara, Niels O. S. [UNIFESP]; Reis, Marlene A.; Correa, Rosana R. M.
    Objective: To elucidate the effects of transcutaneous electrical nerve stimulation (TENS) in pregnancies with placental insufficiency.Methods: Pregnant rats were subjected to uterine artery ligation and to TENS according to the following groups: ligated stimulated (LS); ligated non-stimulated (LN), control stimulated (CS); and control non-stimulated (CN). Fetal external measurements, such as crown-rump length (CRL), fronto-occipital distance (FOD), thoracic ventral-dorsal (TVDD) and abdominal ventral-dorsal (AVDD) distances were analyzed together with the area occupied by fetal internal organs. Glucose transporter 1 (GLUT-1) expression was evaluated by immunohistochemistry in fetal organs. Thickness of junctional, labyrinth and intermediate placental zones was analyzed by morphometric evaluation in HE-stained slides, and placental hypoxia-inducible factor 1 alfa expression was measured by real-time polymerase chain reaction.Results: In LN and CS groups compared to the CN group, CRL was reduced (27.51/28.95 versus 30.16mm), as well as FOD (6.63/6.63 versus 7.36mm), AVDD (7.38/8.00 versus 8.61mm) and TVDD (6.46/6.87 versus 7.23mm). Brain GLUT-1 expression was higher in LS (1.3%) and CS (1.8%). The area occupied by placental vessels in the labyrinth zone (29.673.51 versus 20.83 +/- 7.63) and intermediate zone (26.46 +/- 10.21 versus 10.86 +/- 8.94) was larger in the LS group than in the LN group.Conclusions: Our results suggest a negative effect of TENS on placental development, thus compromising the maintenance of adequate blood flow to the fetus.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Effects of transcutaneous electrical nerve stimulation on fetal and placental development in an experimental model of placental insufficiency
    (Taylor & Francis Ltd, 2016) Guimaraes, Camila S. O.; Gomes, Bruno B. F.; Oliveira, Rafael A.; Yamamoto, Leandro R.; Rocha, Laura P.; Gloria, Maria A. [UNIFESP]; Machado, Juliana R.; Camara, Niels O. S. [UNIFESP]; Reis, Marlene A.; Correa, Rosana R. M.
    Objective: To elucidate the effects of transcutaneous electrical nerve stimulation (TENS) in pregnancies with placental insufficiency.Methods: Pregnant rats were subjected to uterine artery ligation and to TENS according to the following groups: ligated stimulated (LS); ligated non-stimulated (LN), control stimulated (CS); and control non-stimulated (CN). Fetal external measurements, such as crown-rump length (CRL), fronto-occipital distance (FOD), thoracic ventral-dorsal (TVDD) and abdominal ventral-dorsal (AVDD) distances were analyzed together with the area occupied by fetal internal organs. Glucose transporter 1 (GLUT-1) expression was evaluated by immunohistochemistry in fetal organs. Thickness of junctional, labyrinth and intermediate placental zones was analyzed by morphometric evaluation in HE-stained slides, and placental hypoxia-inducible factor 1 alfa expression was measured by real-time polymerase chain reaction.Results: In LN and CS groups compared to the CN group, CRL was reduced (27.51/28.95 versus 30.16mm), as well as FOD (6.63/6.63 versus 7.36mm), AVDD (7.38/8.00 versus 8.61mm) and TVDD (6.46/6.87 versus 7.23mm). Brain GLUT-1 expression was higher in LS (1.3%) and CS (1.8%). The area occupied by placental vessels in the labyrinth zone (29.673.51 versus 20.83 +/- 7.63) and intermediate zone (26.46 +/- 10.21 versus 10.86 +/- 8.94) was larger in the LS group than in the LN group.Conclusions: Our results suggest a negative effect of TENS on placental development, thus compromising the maintenance of adequate blood flow to the fetus.