Navegando por Palavras-chave "ovary"

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    Efeitos da melatonina no sistema genital feminino: breve revisão
    (Assoc Medica Brasileira, 2008-05-01) Maganhin, Carla Cristina [UNIFESP]; Carbonel, Adriana Aparecida Ferraz [UNIFESP]; Hatty, Juliana Halley [UNIFESP]; Fuchs, Luiz Fernando Portugal [UNIFESP]; De Oliveira-Junior, Itamar Souza [UNIFESP]; Simoes, Manuel de Jesus [UNIFESP]; Simoes, Ricardo S. [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Soares-Jr, Jose Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Melatonin is secreted by the pineal gland and this is linked to the day/night cycle. It is an antioxidant and plays a fundamental role in the regulation of the jet-lag stage, in several physiological reactions and in control of the biologic rhythm, Human melatonin has an Important influence on the female genital system. in fact, melatonin may influence production and action of steroids, modifying cellular signalization on the target tissue. There are many evidences that the melatonin therapy may be interfering with neoplasia development, mainly of the estrogen-dependent tumor. This paper aims to analyze the actions of melatonin on the neuroendocrine, immunological and cardiovascular systems, as well as on the reproductive function.
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    Effects of hexestrol on mouse ovarian morphology and ovulation
    (Elsevier B.V., 2008-06-20) Oliveira, Joaquim Moraes de [UNIFESP]; Simoes, Manuel J. [UNIFESP]; Mora, Oswaldo Alves [UNIFESP]; Simoes, Ricardo Santos [UNIFESP]; Oliveira-Filho, Ricardo M. [UNIFESP]; Oliveira, Patricia B. [UNIFESP]; Baracat, Edmund C. [UNIFESP]; Soares, Jose Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To analyze histological aspects of ovaries as well as the ovulation of adult mice treated with the anabolic agent hexestrol.Methods: Thirty adult mice were divided into three groups of 10 animals each: (GI) the animals received a dose of 3 mg/kg of hexestrol; (GII) the animals were given a dose of 6 mg/kg of hexestrol; (GIII) the animals were injected with distilled water (vehicle). Another 10-animal group (GIV) was included, and these mice were injected with propionate testosterone (1.25 mg) after 5 days from the day of birth. Hexestrol was administered intraperitoneally once a day and the treatment lasted 30 days. the mice were then sacrificed; their ovaries and oviducts were removed, submitted to histological routine and analyzed under light microscopy.Results: in mice treated with hexestrol (6 mg/kg) (Group II), ovaries were smaller than those from the controls but highly vascularized; similar results were obtained in GIV. A great number of follicles in several stages of development were found however, with no corpora lutea - in six animals in GII No corpora lutea were found in GIV. the number of luteal bodies and oocytes in GII was lower than that in GI or GIII. No oocytes were detected in GIV. Finally, the nuclear volume of interstitial cells in GII and GIV was the largest.Conclusion: Our data suggest that the anabolic agent hexestrol in a high dose may decrease ovulation in mice. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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    Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles
    (Wiley-Blackwell, 2005-03-01) Buratini, J.; Glapinski, V. F.; Giometti, I. C.; Teixeira, A. B.; Costa, I. B.; Avellar, MCW; Barros, C. M.; Price, C. A.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Univ Montreal
    Paracrine cell signaling is thought to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family have been suggested. in the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR-3c and FGFR-4) are expressed in bovine preantral follicles. Reverse transcription-polymerase chain reaction was used to amplify bovine FGF-8, FGFR-3c, and FGFR-4 from preantral follicle samples and a variety of fetal and adult tissues. All three genes were widely expressed in fetal tissues, with a restricted expression pattern in adult tissues. FGF-8 and FGFR-3c were expressed in secondary follicles in 70% of fetuses examined, whereas FGFR-4 expression was significantly less frequent (20%). FGFR-3c expression frequency was significantly lower in primordial compared to secondary follicles, and FGF-8 expression showed a similar trend. FGFR-4 was only observed when all follicle classes of an individual were expressing both FGF-8 and FGFR-3c. We conclude that FGF-8 and its receptors are expressed in preantral follicles in a developmentally regulated manner. (C) 2005 Wiley-Liss, Inc.
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    Immunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary
    (Associação Médica Brasileira, 2014-07-01) Lombardi, Leonardo Augusto; Simões, Ricardo Santos; Maganhin, Carla Cristina [UNIFESP]; Baracat, Maria Cândida Pinheiro; Silva-Sasso, Gisela Rodrigues; Florencio-Silva, Rinaldo [UNIFESP]; Soares Júnior, José Maria [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); University of São Paulo Medical School Obstetrics and Gynecology Department
    Objective:to evaluate the immunohistochemical expression of proliferative, apoptotic and steroidogenic enzyme markers in the ovaries of rats with polycystic ovary syndrome (PCOS).Methods:twenty rats were divided into two groups: GCtrl - estrous phase, and PCOS - with polycystic ovaries. The GCtrl animals were subjected to a lighting period from 7 am to 7 pm, while the animals with PCOS group remained with continuous lighting for 60 days. Subsequently, the animals were anesthetized, the ovaries were removed and fixed in 10% formaldehyde, prior to paraffin embedding. Sections were stained using H.E. or subjected to immunohistochemical methods for the detection of Ki-67, cleaved caspase-3, CYP11A1, CYP17A1 and CYP19A1. The results were analyzed using Student's t-test (p < 0,05).Results:morphological results showed evidence of interstitial cells originating from the inner theca cells of degenerating ovarian cysts in PCOS. Immunoexpression of Ki-67 was higher in the granulosa cells in GCtrl, and the theca interna cells in PCOS, while cleaved caspase-3 was higher in granulosa cells of ovarian cysts from PCOS and in the theca interna cells of GCtrl. Immunoreactivity of CYP11A1 in the theca interna, granulosa and interstitial cells was similar between the two groups, while CYP17A1 and CYP19A1 were higher in the granulosa and interstitial cells in the PCOS group.Conclusion:the results indicate that the interstitial cells are derived from the theca interna and that enzymatic changes occur in the theca interna and interstitial cells in ovaries of rats with PCOS, responsible for the high levels of androgens and estradiol.
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    Influence of ovarian hormones deprivation on gene expression in the lower urinary tract of rats
    (Sociedade Brasileira de Urologia, 2007-08-01) Bortolini, Maria Augusta Tezelli [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Hamerski, Maria G. [UNIFESP]; Castro, Rodrigo de Aquino [UNIFESP]; Sartori, Marair Gracio Ferreira [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    OBJECTIVE: Identify the influence of ovarian hormone deprivation in expression genes on the lower urinary tract of rats. MATERIALS AND METHODS: This study deals with gene screening on lower urinary tract of rats. Fifty isogenic rats divided in two groups of twenty-five animals have their lower urinary tract surgically removed: group I, ovariectomized rats 30 days prior to surgery; group II, non-ovariectomized rats. Total RNA was isolated from bladder and urethra, and differential expression of genes was analyzed quantitative, qualitative and comparatively by array technology and RT-PCR. RESULTS: A total of 76 candidate genes were identified as differentially expressed between the groups, 26 being lower expressed in group II, and 50 in group I. Among them, differential expression validation was confirmed by RT-PCR for three lower expressed genes in group I: Vascular Endothelial Growth Factor (VEGF), Beta-2 Microglobulin (B2M) and Cytochrome c Oxidase subunit I (COX I). CONCLUSION: Ovarian hormone deprivation influences the expression genes on lower urinary tract. We demonstrated that a 30-day period of castration down regulate the expression of VEGF, B2M and COX I in adult rats which are involved in activities of angiogenesis, immune responses and cellular metabolism respectively.
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    Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats
    (Elsevier B.V., 2014-07-01) Maganhin, Carla C. [UNIFESP]; Simoes, Ricardo S.; Fuchs, Luiz F. P.; Sasso, Gisela R. S. [UNIFESP]; Simoes, Manuel J. [UNIFESP]; Baracat, Edmund C.; Soares, Jose M.; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    Objective: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats.Design: Experimental research.Setting: University research laboratory.Animal(s): Thirty female adult rats. Intervention(s): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 mu g/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary.Main Outcome Measure(s): Biologic molecular study followed by immunohistochemical analysis.Result(s): the changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups.Conclusion(s): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. (C)2014 by American Society for Reproductive Medicine.
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    Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy
    (Oxford Univ Press, 2004-06-01) Almodin, Carlos Gilberto [UNIFESP]; Minguetti-Camara, V. C.; Meister, H.; Ferreira, JOHR; Franco, R. L.; Cavalcante, A. A.; Radaelli, MRM; Bahls, A. S.; Moron, Antonio Fernandes [UNIFESP]; |Murta, Carlos Geraldo Viana [UNIFESP]; Materbaby Reprod Humana & Genet; Universidade Estadual de Maringá (UEM); Universidade Federal de São Paulo (UNIFESP); Univ Fed Espirito Santo
    BACKGROUND: Many cancer survivors face infertility as a consequence of the aggressive treatment they must undergo. Cryopreservation of ovarian tissue before chemotherapy or radiotherapy may allow for tissue transplantation after the treatment, and restoration of fertility. We tested the potential of an orthotopic autografting of cryopreserved germinative tissue in female rabbits with ovarian failure following radiotherapy. METHODS: Ten adult multiparous female rabbits were randomly allocated into two groups, five in group I (control) and five in group II (transplant). All rabbits underwent right oophorectomy with cryopreservation of the germinative tissue, followed by sterilization of the remaining left ovary by radiotherapy. Later, group II rabbits received in the irradiated left ovary an implant of the frozen germinative tissue from the right ovary, whose small pieces were freely spread intracortically in a procedure we named 'intracortical sowing of germinative tissue' (ISGT). RESULTS: All group II rabbits conceived following spontaneous mating within 6 months of the transplant, whereas none of the remaining rabbits in group I had conceived up to 11 months after transplant. CONCLUSIONS: This study suggests that fertility can be restored in rabbits by sowing cortical tissue in a previously irradiated ovary. the clinical feasibility of this technique remains to be determined.