Navegando por Palavras-chave "oligozoospermia"

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    Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
    (Elsevier B.V., 2006-09-01) Paula, Thais Serzedello de [UNIFESP]; Bertolla, Ricardo Pimenta [UNIFESP]; Spaine, Deborah Montagnini [UNIFESP]; Cunha, Maria Adelaide [UNIFESP]; Schor, Nestor [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To verify of the increase in sperm apoptotic DNA fragmentation during cryopreservation is greater in oligozoospermic patients than in normozoospermic controls.Design: Controlled prospective study.Setting: patients in an academic research environment.Patient(s): Forty-seven patients with oligozoospermia (concentration < 10 x 10(6) sperm/mL) and 30 normozoospermic men.Intervention(s): Sperm cryopreservation using a standard protocol with a test-yolk buffer, glycerol as the cryoprotectant.Main Outcome Measure(s): Rate of apoptotic sperm DNA fragmentation as assessed by the terminal deoxynucleotidyl-mediated deoxyuridine triphosphate nickend labeling (TUNEL) assay, graded in apoptotic or nonapoptotic, before and after cryopreservation.Result(s): An increase in apoptotic DNA fragmentation was observed in all the groups, regardless of sperm concentration. Normozoospermic men presented a smaller rate of apoptotic DNA fragmentation than oligozoospermic patients, both in pre- and postcryopreservation samples. the increase in DNA fragmentation was similar in both groups.Conclusion(s): Cryopreservation induces apoptotic sperm DNA fragmentation in men, regardless of sperm concentration. Men with oligozoospermia present with higher precryopreservation and postcryopreservation apoptotic sperm DNA fragmentation. the increase in DNA fragmentation is similar in both groups.
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    Effects of acupuncture and moxa treatment in patients with semen abnormalities
    (Science China Press, 2003-12-01) Gurfinkel, Edson [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Yamamura, Ysao [UNIFESP]; Srougi, Miguel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Aim: To evaluate the effect of Chinese Traditional Medicine, acupuncture and moxa treatment, on the semen quality in patients with semen abnormalities. Methods: In a prospective, controlled and blind study, nineteen patients, aged 24 years similar to 42 years and married for 3 years similar to 11 years without children with semen abnormalities in concentration, morphology and/or progressive motility without apparent cause, were randomized into two groups and submitted to acupuncture and moxa treatment at the therapeutic (Study Group) and the indifferent points (Control Group), respectively, for 10 weeks. Semen analyses were performed before and after the treatment course. Results: The patients of the Study Group presented a significant increase in the percentage of normal-form sperm compared to the Control Group (calculated U = 16.0, critical U = 17.0). Conclusion: The Chinese Traditional Medicine acupuncture and moxa techniques significantly increase the percentage of normal-form sperm in infertile patients with oligoastenoteratozoospermia without apparent cause. (Asian J Androl 2003 Dec; 5: 345-348).
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    Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
    (Oxford Univ Press, 2006-07-01) Cedenho, Agnaldo Pereira [UNIFESP]; Lima, Samira Barbosa [UNIFESP]; Cenedeze, Marcos Antonio [UNIFESP]; Spaine, Deborah Montagnini [UNIFESP]; Ortiz, Valdemar [UNIFESP]; Oehninger, S.; Universidade Federal de São Paulo (UNIFESP); Jones Inst Reprod Med
    BACKGROUND: Heat-shock protein A2 (HspA2) is correlated with sperm maturity, function and fertility, and a dysfunctional expression of such a gene results in abnormal spermatogenesis. the purpose of this study was to compare HspA2 gene expression in spermatozoa from oligozoospermic men and normozoospermic controls. METHODS: Semen was obtained and analysed according to World Health Organization (World Health Organization, 1999) guidelines, morphology by Kruger's strict criteria. Seventeen patients with oligozoospermia and 21 fertile controls were studied. Total RNA was extracted from ejaculated and Percoll density-gradient-separated spermatozoa followed by semiquantitative RT-PCR analysis. the relative expression level of HspA2 was analysed according to the expression level of the housekeeping beta-actin gene. Serum hormonal profiles (FSH, LH and testosterone) and a peripheral karyotype were also performed. RESULTS: All patients possessed normal karyotype, and no significant hormonal differences were found between the two groups. the study group had significantly lower sperm concentration and normal morphology than the controls. Semiquantitative RT-PCR analysis of HspA2 showed significantly lower expression levels in the oligoteratozoospermic men when compared to controls (P = 0.0021). CONCLUSIONS: the HspA2 gene was down-regulated in sperm from infertile men with idiopathic oligoteratozoospermia, suggesting that such anomalies of gene expression might be associated with pathogenesis in some subtypes of male infertility.