Navegando por Palavras-chave "morphometry"
- ItemSomente MetadadadosCorneal endothelial cell morphology of normal dogs in different ages(Blackwell Publishing, 2006-03-01) Rodrigues, G. N.; Laus, J. L.; Santos, J. M.; Rigueiro, M. P.; Smith, R. L.; UNESP; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Endothelial cell function is essential to maintain corneal transparency, but unfortunately the regenerative capacity of the endothelium is limited. There are only a few reports describing the effect of age on morphologic appearance of corneal endothelial cells of dogs. Studies of normal corneal endothelial cells in humans and dogs have shown a decrease in endothelial cell density (ECD) and an increase in pleomorphism and polymegethism with advancing age. the purpose of this study was to investigate the effect of age on ECD and endothelial cell morphology in dogs. A total of 30 dogs were divided into three groups (10 dogs/group) based on age: group 1 (2-12 months old), group 2 (24-72 months old), and group 3 (84 months or older). Corneas were processed for light and scanning electron microscopy. Results showed only difference in cell density between group 1 and groups 2 and 3, showing an initial decrease in cell density as the animal matured. Whereas there was significantly greater variation in cell size within the dogs in group 3 than there was within the other two groups, suggesting that there was increased polymegethism and pleomorphism with advancing age.
- ItemSomente MetadadadosEffect of ultrasound therapy on the repair of Gastrocnemius muscle injury in rats(Elsevier B.V., 2008-09-01) Balejo Piedade, Maria Cristina [UNIFESP]; Galhardo, Milene Sanches [UNIFESP]; Battlehner, Claudia Naves; Ferreira, Marcelo Alves; Caldini, Elia Garcia; Szymanski de Toledo, Olga Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)The aim of this study was to evaluate the effect of the pulsed ultrasound therapy (PUT) in stimulating myoregeneration and collagen deposition in an experimental model of lacerative gastrocnemius muscle lesion in 30 Wistar rats. Fifteen rats were treated (TG) daily with 1 MHz pulsed ultrasound (50%) at 0.57 W/cm(2) for 5 min, and 15 were control animals (CG). Muscle samples were analyzed on postoperative days 4, 7 and 14 through H&E, Picrosirius-polarization and immunohistochemistry for desmin. the lesions presented similar inflammatory responses in both treated and control groups. the areal fraction of fibrillar collagen was larger in the TG at 4 days post-operatively (17.53 +/- 6.2% vs 6.79 +/- 1.3%, p = 0.0491), 7 days (31.07 +/- 7.45% vs 12.57 +/- 3.6%, p = 0.0021) and 14 days (30.39 +/- 7.3% vs 19.13 +/- 3.51%, p = 0.0118); the areal fraction of myoblasts and myotubes was larger in the TG at 14 days after surgery (41.66 +/- 2.97% vs 34.83 +/- 3.08%, p = 0.025). Our data suggest that the PUT increases the differentiation of muscular lineage cells, what would favor tissue regeneration. On the other hand, it is also suggested that there is a larger deposition of collagenous fibers, what could mean worse functional performance. However, the percentage of fibers seems to have stabilized at day 7 in TG and kept increasing in CG. Furthermore, the collagen supramolecular organization achieved by the TG is also significant according to the Sirius red staining results. (C) 2008 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosGranular cell dispersion and bilamination: two distinct and bilamination, histopathological patterns in epileptic hippocampi?(John Libbey Eurotext Ltd, 2007-12-01) Silva, Alexandre Valotta da [UNIFESP]; Houzel, Jean-Christophe; Croaro, Ingrid [UNIFESP]|Yacubian, Elza Márcia Targas [UNIFESP]; Stávale, João Norberto [UNIFESP]; Centeno, Ricardo Silva [UNIFESP]; Cavalheiro, Esper Abrão [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Rio de Janeiro (UFRJ)Cytoarchitectural modifications of the dentate gyrus are among the most obvious abnormalities observed in the hippocampal sclerosis associated with refractory epilepsy. Here, we examined the morphological changes of granular cells (dispersion, bilamination and cell loss) in sclerotic hippocampi from nine TLE patients, comparing abnormal and preserved areas. A total of 2 577 granular cells were analyzed with respect to four different histopathological patterns: areas with bilamination (n = 936), areas with dispersion (n = 905), areas with neuronal loss (n = 279), and preserved areas (n = 457). Quantitative parameters included somatic perimeter (P), area (A) and form factor (ff).Although different patterns were often observed in the same patient, highly significant differences were observed (p < 0.0001) when patterns were compared to one another. Since granular cell dispersion and bilamination have different morphological aspects in sclerotic hippocampi from TLE patients, we suggest that these patterns should be considered separately. Future studies are needed to determine the frequency with which these patterns occur in the general population and whether each one can interfere with seizure susceptibility.
- ItemSomente MetadadadosGrape juice concentrate alleviates epididymis and sperm damage in cadmium-intoxicated rats(Wiley, 2017) Lamas, Celina de A.; Cuquetto-Leite, Livia; da Silva, Emanueli do Nascimento; Thomazini, Bruna F.; Cordeiro, Gabriel da S.; Predes, Fabricia de S.; Golluecke, Andrea P. B. [UNIFESP]; Dolder, HeidiThe possibility of long-term grape juice concentrate (GJC) consumption conferring a protective effect against cadmium (Cd)-induced damage to the epididymis, completely preserving sperm profile, was evaluated here for the first time in the scientific literature. Male Wistar rats (n=6/per group) received an intraperitoneal Cd injection (1.2mg/Kg) at age 80days and GJC (2g/Kg) by gavage from 50days until 136days old. Groups receiving either Cd or GJC were added. An intraperitoneal injection of saline (0.9%) and water by gavage was administered in the absence of treatment with Cd or GJC. Animals were anaesthetized and exsanguinated at 136days
- ItemSomente MetadadadosHistochemical and ultrastructural study of the extracellular matrix fibers in patellar tendon donor site scars and normal controls(Editrice Compositori Bologna, 1996-04-01) Battlehner, Cláudia Naves; Carneiro Filho, Mario [UNIFESP]; Ferreira Junior, Jorge Mario da Costa; Saldiva, Paulo Hilário Nascimento [UNIFESP]; Montes, Gregorio Santiago; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); BUTANTAN INSTLesions of the anterior cruciate ligament are extremely common and frequently demand surgical treatment in order to avoid disabling sequels. The use of a central one-third of the patellar tendon as an autograft for surgical reconstruction of a damaged cruciate ligament is common. Although several investigations in human and animal models have demonstrated long-term graft viability, there have been cases of loosening and rupture of the graft. In these occasions, a new substitute for the torn structure must be found. Owing to its inherent accessibility, the patellar tendon has been elected one of the choices of donor tissue. In order to evaluate the characteristics of the remaining scar, we performed a histochemical and ultrastructural study using biopsy material obtained from the central one-third of the donor tendon of 8 patients. This material was analyzed by comparing the ultrastructural picture with the results obtained using the specific method for collagen-containing fibers (Picrosirius-polarization) by light microscopy; four normal patellar tendons were used as controls. Despite the resemblance with the normal tissue, our results show that Phr healed tissue does nor restore the tendon ad integrum, neither at the light microscopic nor at the electron microscopic levels. Structural differences can be responsible for biomechanical alterations. Impaired biomechanical properties can, at least partly, explain some of the clinical complications observed in patients submitted to this surgical technique. However, without performing biomechanical studies in this kind of tendons, we are neither allowed to encourage nor to reject the use of scars as donor tissue for a second surgery.
- ItemSomente MetadadadosHistomorphometry of sexually immature albino rat testis after X ray-irradiation(Interciencia, 1997-03-01) Cabral, Maria das Gracas Botelho [UNIFESP]; Hayashi, Hisakazu [UNIFESP]; Miraglia, Sandra Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)A A morphometric study was conducted of sexually immature albino rat testes which were exposed to a localized single. X-ray dose of 154.00 mC/Kg.The rats were killed at the ages of 25, 30, 35, 45, 60, 90 and 120 days. Their testes were removed from the scrota and examined microscopically by light microscope and macroscopically, To obtain better identification of all phases of spermatogenesis, 6 mu m-thick paraffin sections were stained using the periodic acid-Schiff (PAS) and Harris hematoxylin methods.The results show that X-rays provoke severe alterations in the seminiferous epithelium, selectively eliminating a significant quantity of the spermatogonia. On other hand, the elements which survived X-irradiation, including some types of spermatogonia and undifferentiated cells, were capable of repopulating the seminiferous epithelium. Animals irradiated at 25 days of age were found to be fertile when they were mated between 90 and 120 days.
- ItemSomente MetadadadosMorphological and functional aspects of acute kidney injury after fetal programing in the offspring of diabetic rats(Informa Healthcare, 2015-03-01) Martins Pucci, Karla Roberta; Pereira Junior, Carlos Donizete; Idalo, Priscila Barbosa; Santana Pinheiro Moreira, Ana Carolina; Rocha, Laura Penna; Aquiles Rodrigues, Aldo Rogelis; Reis, Luiz Carlos dos; Silva Gomes, Roseli A. da; Rocha, Lenaldo Branco; Oliveira Guimaraes, Camila Souza de; Reis, Marlene Antonia dos; Saraiva Camara, Niels Olsen [UNIFESP]; Miranda Correa, Rosana Rosa; Univ Fed Triangulo Mineiro; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)Objective: To evaluate the effects of folic acid (FA)-induced renal failure in young offspring of diabetic mothers.Methods: the offspring of streptozotocin-induced diabetic dams were divided into four groups: CC (controls receiving vehicle); DC (diabetics receiving vehicle); CA (controls receiving FA solution, 250 mg/kg) and DA (diabetics receiving FA solution, 250 mg/kg). Renal function tests and morphometry results were analyzed.Results: An increase in creatinine and urea levels was observed in CA and DA groups at two and five months. FA administration caused a significant reduction in the number of glomeruli in the offspring of diabetic dams. the diabetes group treated with FA had fewer glomeruli compared to controls at two and five months. FA caused an increase in the area of the urinary space both in controls and offspring of diabetic dams at two and five months. the number of glomeruli and area of the urinary space at two months were negatively correlated.Conclusions: Fetal programing promotes remarkable changes in kidney morphology and function in offspring. We suggest that the morphological changes in the kidneys are more pronounced when fetal programing is associated with newly acquired diseases, e.g. renal failure induced by FA.
- ItemSomente MetadadadosMorphometrical and immunohistochemical study of intrahepatic bile ducts in biliary atresia(Lippincott Williams & Wilkins, 2011-09-01) Collarile Yamaguti, Diva Carvalho [UNIFESP]; Silva Patricio, Francy Reis da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Background Extrahepatic biliary atresia results from a progressive destruction of the bile ducts by an inflammatory fibrosing process which leads ultimately to cirrhosis of biliary type. the etiology of the disorder remains unknown. the histological features include cholestasis, ductular proliferation, eventual loss of intrahepatic bile ducts, and ducts with primitive embryonic shape (ductal plate malformation).Purpose To examine the morphological changes of the biliary intrahepatic ducts, we aimed at investigating the cell proliferation and the diameter of the interlobular bile ducts in extrahepatic biliary atresia, and in normal liver children.Methods Liver samples from 35 patients with biliary atresia and 10 from control normal children were used. Immunoexpression of cytokeratin 19 was evaluated and a double-staining procedure was performed with cytokeratin 8/proliferating cell nuclear antigen. the stereological measurements of the intrahepatic bile ducts diameter were evaluated by a computerized system of image analysis.Results the patterns of intrahepatic cholangiopathy in biliary atresia were obstructive features (42.86%), paucity of intrahepatic bile ducts (20%), ductal plate malformation (28.57%), and ductal plate malformation associated with paucity of intrahepatic bile ducts (8.57%). the average external diameter of interlobular bile ducts in biliary atresia was smaller than that of the control infant livers. Among the four patterns of biliary atresia cholangiopathies, those associated with ductopenia showed the smallest bile duct diameter. There was a negative correlation between the bile duct to portal space ratio and the age of the child at the time of Kasai portoenterostomy. Only in biliary atresia are the bile duct cells stained with proliferating cell nuclear antigen.Conclusion (i) in biliary atresia, both ductular metaplasia and ductular proliferation were observed; (ii) biliary atresia associated with ductopenia showed narrowing of interlobular ducts, probably as a consequence of degeneration with atrophy and fibrosis. Eur J Gastroenterol Hepatol 23: 759-765 (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.
- ItemSomente MetadadadosMorphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus(Assoc Bras Divulg Cientifica, 1997-05-01) Maifrino, Laura Beatriz Mesiano [UNIFESP]; Prates, José Carlos [UNIFESP]; DeSouza, R. R.; Liberti, E. A.; Universidade de São Paulo (USP); INST DANTE PAZZANESE CARDIOL; Universidade Federal de São Paulo (UNIFESP)The myenteric plexus of the digestive tract of the wild mouse Calomys callosus was examined using a histochemical method that selectively stains nerve cells, and the acetylcholinesterase (AChE) histochemical technique in whole-mount preparations, Neuronal density was 1,500 +/- 116 neurons/cm(2) (mean +/- SEM) in the esophagus, 8,900 +/- 1,518 in the stomach, 9,000 +/- 711 in the jejunum and 13,100 +/- 2,089 in the colon, The difference in neuronal density between the esophagus and other regions was statistically significant, The neuron profile area ranged from 45 to 1,100 mu m(2). The difference in nerve cell size between the jejunum and other regions was statistically significant. AChE-positive nerve fibers were distributed within the myenteric plexus which is formed by a primary meshwork of large nerve bundles and a secondary meshwork of finer nerve bundles, Most of the nerve cells displayed AChE activity in the cytoplasm of different reaction intensities. These results are important in order to understand the changes occurring in the myenteric plexus in experimental Chagas' disease.
- ItemSomente MetadadadosMorphometry of the human bicipital groove (sulcus intertubercularis)(Elsevier B.V., 2010-01-01) Wafae, Nader; Atencio Santamaria, Luciany Everardo; Vitor, Leonardo [UNIFESP]; Pereira, Luiz Antonio; Ruiz, Cristiane Regina; Wafae, Gabriela Cavallini; Ctr Univ Sao Camilo; Universidade Federal de São Paulo (UNIFESP); Univ Nove de JulhoBackground: the bicipital groove is located between the greater and lesser tubercles at the proximal extremity of the humerus and extends inferiorly. Citations that anatomic variations of the groove could give rise to sliding of the biceps brachii muscle tendon led us to initiate a morphometric study on this groove.Materials and methods: the study was done on 50 dry humeral bones from adults of both genders and from both sides. A digital caliper rule was used to measure the length, thickness and width of the bicipital groove and the humerus. the angles of the bicipital groove were measured with a goniometer.Results: the average length of the groove was 8.1 cm and it corresponded to 25.2% of the length of the humerus. the width at the midpoint of the groove was 10.1 mm and corresponded to 49.7% to 54.5% of the width of the humerus. the depth was 4.0 mm and corresponded to 18.8% of the depth of the humerus. the mean angle formed by the groove lips was 106 degrees.Conclusion: This study confirmed the variability of the measurements presented by the groove in relation to all the aspects considered.
- ItemSomente MetadadadosPotential definition of the time of death from autolytic myocardial cells: a morphometric study(Elsevier B.V., 1999-10-11) Munoz, D. R.; Almeida, M. de; Lopes, E. A.; Iwamura, ESM; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Morphometric methods and transmission electron microscopy were used to quantify modifications occurring in the mitochondria of dog myocardium during the first four hours of autolysis. Myocardial fragments were obtained from the outer free wall of the left ventricle, during anesthesia (control-zero) and at 15, 45, 120, and 240 min after cardiac arrest, maintaining the heart in situ at 22 degrees C. During the 240 min of autolysis, the main parameters evaluated showed: (a) a decrease in the number of mitochondria from 0.31 to 0.12 per mu m(3) of cytoplasm. the decrease over the first 45 min reached 50% of the initial value; (b) an increase in mitochondrial volume, three times greater after the first 45 min (from 0.92 to 2.68 mu m(3)) and four times greater after 240 min (from 0.92 to 3.79 mu m(3)); (c) an increase in mitochondrial outer membrane surface area from 5.51 to 12.54 mu m(2); (d) an increase in the surface area of individual mitochondria inner membrane and cristae from 27.60 to 56.96 mu m(2). the progressive nature of the alterations and the difference in the numerically expressed values allow correlation with the time of somatic death. the authors emphasize the need for further studies in order to complement the present study. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.