Navegando por Palavras-chave "metaloproteinase"

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    Matriz Metaloproteinase 2: um importante marcador genético para colesteatomas
    (ABORL-CCF Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial, 2007-02-01) Morales, Douglas Salmazo Rocha; Penido, Norma de Oliveira [UNIFESP]; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Stávale, João Norberto [UNIFESP]; Guilherme, Arnaldo [UNIFESP]; Fukuda, Yotaka [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Hospital do Servidor Público Estadual de São Paulo
    AIM: This study is to determine the MMP2 s presence in cholesteatomas and whether complicating cholesteatomas show a higher immunohistochemical expression of matrix metalloproteinase 2. Cholesteatoma produces enzymesthat cause bone erosion like Matrixmetalloproteinase 2 (MMP2). MATERIAL AND METHODS: We analyzed the expression of MMP2 in invasive (causing complications) compared to latent cholesteatomas (not causing complications). A crosssectional study with nineteen slides and paraffin blocks of cholesteatomas derived from mastoidectomies were located and processed, including 8 invasive and 11 latent cholesteatomas. Immunohistochemical thecnique was empregated to MMP2. RESULTS: The results are expressed as 0, + (to low), ++ and +++(high) according to the quantity and color of the immunohistochemical staining of MMP2. Higher expression of MMP2 was observed in 7 (87.5%) of the 8 invasive cholesteatomas. With respect to latent cholesteatomas, higher expression of MMP2 was observed in 27.3% (3 cases), with Fisher s exact test indicating a significant difference (p=0.015). CONCLUSIONS: Cholesteatoamas express MMP2 and Invasive cholesteatomas had high MMP2 compared to latent cholesteatomas.
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    Tumor de células gigantes do osso: correlação entre a imunoexpressão de metaloproteinases (mmp 2 e 9) e parâmetros clínicos e radiológicos
    (Universidade Federal de São Paulo (UNIFESP), 2016-08-09) Bellan, Davi Gabriel [UNIFESP]; Alves, Maria Teresa de Seixas Alves [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The Giant Cell Tumor of Bone (GCT) is a benign bone tumor, with local aggressive behavior and potential to produce distant metastases. It occurs in the epiphysis of long bones, pelvis and sacrum. Despite the recent emergence of drug therapies, treatment of TGC is still essentially surgical with large resections or intra-lesional depending on their location and stage at diagnosis, but at high risk of local recurrence after piece meal ressection of the lesion. Recent immunohistochemical studies on the GCT demonstrate the presence of various proteins and metalloproteinases with variable expression in the tumor cell surface. It is believed that the expression pattern of such substances may correlate with clinical outcome and the risk of local relapse. Objective: To identify the expression pattern of metaoproteinases (MMPs) in Giant Cell Tumor Bone biopsy samples registered in the Department of Pathology of the Federal University of São Paulo - UNIFESP and correlate them with the clinical and radiological evolution of the patients. Methods: A retrospective study of 33 patients with GCT bone through immunohistochemical study of slides stored in the department of Phatologi to check the pattern of expression of MMP2 and MMP9 through imaging method. Quantitative evaluation of this expression by Image J. software and correlate with local recurrence, metastasis, clinical and epidemiological data. Results: We found a statistically significant relationship between the mean values of immunostaining of MMP9 and local recurrence (p = 0.036) and similar relationship between the mean values of MMP2 with statistical trend (p = 0.064). We also observed that the mean and median MMP2 are correlated decreasing and significant way to the age of patients at diagnosis (p = 0.029; p = 0.010). There was no relationship between MMPs and other clinical, imaging or epidemiological criteria. Conclusion: We conclude that there is a prognostic relation between quantitative values of average MMP9 immunostaining and the tumor local recurrence.