Repositório Institucional - Unifesp :: Navegando por Palavras-chave "lymphocytes"

Navegando por Palavras-chave "lymphocytes"

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Adjuvant effect of LPS and killed Propionibacterium acnes on the development of experimental gastrointestinal nematode infestation in sheep
(Wiley-Blackwell, 2009-10-01) Abel, L. C. J.; Chen, S.; Ricca, L. G.; Martins, M. F.; Garcia, M.; Ananias, R. Z. [UNIFESP]; Mussalem, J. S. [UNIFESP]; Squaiella, Carla Cristina [UNIFESP]; Shaw, R. J.; Longo-Maugéri, Ieda Maria [UNIFESP]; Univ Estadual Paulista; Universidade Federal de São Paulo (UNIFESP); AgRes Grasslands Res Ctr
P>Gastrointestinal helminthic infection is an important worldwide sheep disease. the emergence of anthelminthic resistance has led to drives to seek new means of therapeutic control of helminthiasis in sheep. Several data demonstrated the adjuvant effect of Propionibacterium acnes on resistance to infection. Herein, we evaluate the adjuvant effect of the commercial suspension containing LPS and P. acnes on experimental helminthiasis. Sheep received three doses of LPS and P. acnes commercial suspension or saline 0 center dot 9% (control group). Both groups received orally Haemonchus contortus infective larvae on day 0. Parasitological, haematological, lymphoproliferation analysis, IL-5 and IgE determination were made once a week until 35th day after infection. Our results revealed increase on packed cell volumes on day 14, in LPS + P. acnes treated group. On 21st and 35th days after infection in the same group occurred increase on circulating eosinophils and lymphocytes, and also in the lymphoproliferative response to mitogen. On 35th day, the faecal eggs peak in LPS + P. acnes treated group was significantly lower than control. A negative correlation between faecal eggs counts and circulating eosinophils in the immunostimulant treated group was also observed. Our findings suggest that LPS + P. acnes suspension can be used as a strategy to control helminthiasis in sheep.
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Apoptosis of leukocytes: Basic concepts and implications in uremia
(Blackwell Science Inc, 2001-02-01) Jaber, Bertrand L. [UNIFESP]; Cendoroglo Neto, Miguel [UNIFESP]; Balakrishnan, Vaidyanathapuram S.; Perianayagam, Mary C.; King, Andrew J.; Pereira, Brian JG; Univ Fed Ceara; Hosp Clin Montevideo; Universidade Federal de São Paulo (UNIFESP); Universidade Federal Fluminense (UFF)
Circulating blood leukocytes have short life expectancies and end their lives by committing programmed cell death or apoptosis. Apoptosis is an active form of cell death that is initiated by a number of stimuli and is intricately regulated. Apoptosis in both excessive and reduced amounts has pathological implications. Evidence suggests that apoptosis may play a role in the pathophysiology of immune dysfunction in uremia. Indeed, accelerated programmed cell death has been observed in lymphocytes, monocytes, and polymorphonuclear leukocytes among patients with chronic renal failure. This may be due in part to the retention of uremic toxins. The aim of this article is to review the evidence for accelerated leukocyte apoptosis, key regulatory apoptotic pathways, and the possible role of this highly organized process in the pathogenesis of immune dysfunction in uremia.
Acesso aberto (Open Access)
Avaliação do efeito da hipotermia por crioimersão corporal, nos neutrófilos e linfócitos sanguíneos de ratos submetidos ao exercício físico agudo
(Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, 2008-12-01) Bachur, José Alexandre; Quemelo, Paulo R.; Bachur, Cynthia A. K.; Domenciano, Julio C.; Martins, Carlos H. G.; Stoppa, Marcos A.; Barros Neto, Turibio Leite de [UNIFESP]; Garcia, Sérgio B.; Universidade de Franca Fisioterapia Unifran. Lab. de Estudos da Recuperação Orgânica e Funcional; Universidade de Franca; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
Systemic stress induced by exercise increases bioactive substances in plasma which leads to neutrophilic mobilization. Cryotherapy causes a decrease in the inflammatory reaction and attenuates high blood perfusion after exercise. The objective of this work was to analyze the influence of cold water immersion (CWI) after acute exercise on neutrophil and lymphocyte mobilization. A control group of rats (AI) was kept at rest and a second group (AII) was submitted to CWI at 10º C for 10 minutes. The animals of Groups BI, BII, BIII and BIV were submitted to acute exercise which consisted in swimming in water at 31º C for 100 minutes with a load equivalent to 5% of the body weight. Groups CI, CII, CIII and CIV were submitted to CWI immediately after acute exercise. The animals were sacrificed at 6 (I), 12 (II), 24 (III) and 48 (IV) hours after the exercise and neutrophil and lymphocyte cells were counted for all groups by optic microscopy. The Student t-test was used for statistical analysis with a significance level of p< 0.05. A significant increase in the number of neutrophils was observed in Groups AII, BI, BII, BIII, BIV, CI, CII and CIII compared to AI. The neutrophil count of the CIV Group was similar to the Control Group. There was a significant drop in the number of lymphocytes in Groups BII, BIII, BIV, CI and CII when compared to Group AI. The lymphocyte count of Groups AII, BI, CIII and CIV were similar to the Control Group. The changes in neutrophil and lymphocyte counts caused by acute exercise were reverted to normal at 24 hours by cold water immersion.
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Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation
(Elsevier B.V., 2005-09-01) Rojas, B.; Cuhna, R.; Zafirakis, P.; Ramirez, J. M.; Lizan-garciia, M.; Zhao, T.; Foster, C. S.; Univ Complutense Madrid; Harvard Univ; Universidade Federal de São Paulo (UNIFESP); Hosp Gen Albacete
We were interested to analyse the composition of the cellular infiltrate and adhesion molecules expression in the conjunctiva before and at least one hundred days after autologous and allogenic bone marrow transplantation (BMT) and its relation with the presence of dry eye. We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T-lymphocytes (CD3, CD4 and CD8), B-lymphocytes (CD19), macrophages (CD14), natural killer cells (NK, CD57), intercellular adhesion molecule 1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), lymphocyte function associated antigen-1 (LFA-1), very late antigen-4 (VLA-4), interleukin 2 receptor (IL2r, CD25) and HLA-DR. Our autologous recipients had no graft-versus-host disease (GVHD) but allogenic patients had chronic GVHD. After autologous BMT the conjunctiva had significantly more: (1) T lymphocytes (CD3+, CD4+, CD8+) in the epithelium; (2) CD4+ and CD 14+ cells in the stroma; and (3) VLA-4 expression in the stroma than before BMT. After allogenic BMT, the conjunctiva exhibited a significant increase of: (1) CD3+ and CD 14+ cells in the epithelium; (2) T lymphocytes (CD3+, CD4+, CD8+) and CD 14+ cells in the stroma; and (3) VLA-4 and LFA-1 expression in the stroma than before BMT. After the engraftment, the comparison between autologous and allogenic recipients revealed that: (1) there were no significant differences in adhesion molecule expression; (2) the epithelium of autologous recipients had significantly more CD3+ cells; and (3) the stroma of allogenic patients had significantly more CD3+ and CD8+ cells. Among allogenic recipients, CD14+ cells were significantly increased both in the epithelium and in the stroma of patients with signs or symptoms of dry eye in comparison with patients without ocular involvement. Additionally, those having keratoconjunetivitis sicca (KCS) had CD4/CD8 ratios significantly higher than those without KCS.In conclusion, in the conjunctiva after autologous BMT a subclinical cell mediated immune reaction seems to take place. the conjunctivitis of chronic GVHD is complex, with T cells and macrophages dramatically contributing to the process. (c) 2005 Elsevier B.V. All rights reserved.
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Exercise prevents the effects of experimental arthritis on the metabolism and function of immune cells
(Wiley-Blackwell, 2010-06-01) Navarro, Francisco; Bacurau, Alinc V. N.; Almejda, Sandro S. [UNIFESP]; Barros, Carlos C. [UNIFESP]; Moraes, Milton R. [UNIFESP]; Pesquero, Jorge L.; Ribeiro, Sandra M. L.; Araujo, Ronaldo C. [UNIFESP]; Costa Rosa, Luis F. B. P.; Bacurau, Reury Frank Pereira; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
Active lymphocytes (LY) and macrophages (M Phi) are involved in the pathophysiology of rheumatoid arthritis (RA) Due to its anti-inflammatory effect. physical exercise may be beneficial in RA by acting on the immune system (IS) Thus, female Wistar rats with type II collagen-induced arthritis (CIA) were submitted to swimming training (6 weeks. 5 days/week. 60 min/day) and some biochemical and immune parameters, such as the metabolism of glucose and glutamine and function of LY and M. were evaluated in addition, plasma levels of some hormones and of interleukin-2 (IL-2) were also determined Results demonstrate that CIA increased lymphocyte proliferation (1.9- and 1 7-fold, respectively, in response to concanavalin A (ConA) and lipopolysaccharide (LPS)), as well as macrophage H(2)O(2) production (1 6-fold), in comparison to control Exercise training prevented the activation of immune cells, induced by CIA. and established a pattern of substrate utilization similar to that described as normal for these cells. Exercise also promoted an elevation of plasma levels of corticosterone (22 2%), progesterone (1 7-fold) and IL-2 (2 6-fold) Our data suggest that chronic exercise is able to counterbalance the effects of CIA on cells of the IS. reinforcing the proposal that the benefits of exercise may not be restricted to aerobic capacity and/or strength improvement Copyright (C) 2010 John Wiley & Sons, Ltd
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Exercise restores immune cell function in energy-restricted rats
(Lippincott Williams & Wilkins, 2004-12-01) Cunha, Wilton Darleans dos Santos; Giampietro, M. V.; Souza, D. F. de; Vaisberg, Mauro [UNIFESP]; Seelaender, MCL; Rosa, LFBPC; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
Objective: To evaluate the effect of chronic moderate-intensity exercise upon the alterations of immune system cell function induced by energy restriction.Methods: Forty male Wistar rats were randomly assigned to the following groups: sedentary animals fed ad libitum (SF, N = 10) or submitted to energy restriction (SER, N = 10, receiving 50% of the mean amount of chow consumed by SF); and trained animals fed ad libitum (TF, N = 10) or submitted to energy restriction (TER, N = 10), who exercised on a treadmill (at 60-65% VO2max) 5 d(.)wk(-1) for 10 wk, after 30 d under the restriction protocol. the incorporation of [2-C-14]-thymidine by lymphocytes obtained from the spleen and mesenteric lymph nodes, plasma glucose and glutamine concentration, and cytokine production by cells cultivated in the presence of glutamine were measured in all groups, 24 h after the last exercise session. Two-way ANOVA and Tukey's posttest were employed for the statistical analysis.Results: Training induced an increase in the proliferative response and in the production of gamma-interferon and interleukin-1 (P < 0.05) in cells from the spleen and lymph nodes of SER, in which these parameters were diminished when compared with SF (P < 0.05). SER spleen and lymph node cells produced more TNF (26 and 42%, respectively) and IL-2 (49 and 42%, respectively) than SF. the Th-1-like diversion of the immune response observed in SER persisted after training. Partial recovery of the decreased SER plasma glutamine concentration and muscle glutamine synthase mRNA was observed.Conclusions: Training induced the recovery of the proliferative capacity of lymphocytes from SER, probably due to the partial restoration of plasma glutamine levels, but did not interfere with the diversion towards a Th-1-type immune response induced by food restriction.
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Heparanase expression in circulating lymphocytes of breast cancer patients depends on the presence of the primary tumor and/or systemic metastasis
(Neoplasia Press, 2007-06-01) Theodoro, Therese Rachell; Matos, Leandro Luongo de [UNIFESP]; Sant'Anna, Aleksandra Vanessa Lambiasi; Fonseca, Fernando Luiz Affonso [UNIFESP]; Semedo, Patricia [UNIFESP]; Martins, Lourdes Conceicao; Nader, Helena Bonciani [UNIFESP]; Del Giglio, Auro; Pinhal, Maria Aparecida da Silva [UNIFESP]; Fac Med ABC; Universidade Federal de São Paulo (UNIFESP)
Heparanase is an endo-beta-glucuronidase that is capable of degrading heparan sulfate chains of proteoglycans, generating a variety of bioactive molecules such as growth factors and chemotactic and angiogenic agents. the expression of heparanase was investigated in the peripheral blood mononuclear cell fraction ( PBMC) of 30 patients with breast cancer and 20 healthy control women by reverse transcription-polymerase chain reaction ( RT-PCR) and immunocytochemistry. PBMC samples from all breast cancer patients at study entry showed the expression of heparanase, whereas no expression was observed for healthy women. Immunocytochemistry analysis demonstrated that heparanase was expressed in lymphocytes of breast cancer PBMC. Throughout follow-up, heparanase expression by RTPCR decreased significantly after surgery in patients treated with neoadjuvant chemotherapy ( P = .002) and after tamoxifen treatment ( P = .040), whereas it increased significantly with the advent of systemic metastasis ( P = .027). in vitro, either serum from breast cancer patients or a medium originated from co-culture experiments of MCF-7 cells and lymphocytes from healthy women stimulated heparanase expression in normal lymphocytes. the results suggest that there is a tumor-inducing effect on heparanase expression by lymphocytes present in the PBMCs of breast cancer patients, which depends, in turn, on the interaction between a tumor and normal lymphocytes.
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Hypogammaglobulinemia in children: a warning sign to look deeply?
(Wiley, 2017) de Melo, Karina Mescouto [UNIFESP]; de Moraes-Pinto, Maria Isabel [UNIFESP]; Andrade, Luis E. C. [UNIFESP]; Salomao, Reinaldo [UNIFESP]; Brunialti, Milena K. C. [UNIFESP]; Ferreira, Vanessa S. [UNIFESP]; Costa-Carvalho, Beatriz T. [UNIFESP]
This study investigated phenotypic and functional characteristics of lymphocytes in children with common variable immunodeficiency (CVID) and unclassified hypogammaglobulinemia (UH), as well as B-cell subsets in non-consanguineous parents. Blood samples of 30 children, CVID (n = 9), UH (n = 9), healthy donors HD (n = 12), and 19 adults (parents and controls) were labeled by a combination of surface markers to identify CD4, CD8 T-cell and B-cell sub-populations. T-cell cytokine production in children was analyzed in vitro after stimulation with phytohemagglutinin (PHA) and tetanus toxoid. We observed low percentages of switched memory B cells in children with CVID, increase in total CD4(+) T-cell counts, and high percentages of transitional B cells only in UH group. Analysis of T-cell immunity showed that CVID children had decreased percentages of CD8(+) IFN-gamma-producing cells after stimulation with PHA and tetanus toxoid. Parent of children with CVID had low percentages of naive B cell and increased percentages of memory B cells in comparison with controls. These results suggest that (i) early combined immune defect in children with CVID and (ii) a possible familial B-cell disturbance in pediatric CVID.
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Immune cellular parameters of leprosy and human immunodeficiency virus-1 co-infected subjects
(Wiley-Blackwell, 2008-06-01) Carvalho, Karina I. [UNIFESP]; Maeda, Solange [UNIFESP]; Marti, Luciana; Yamashita, Jane [UNIFESP]; Haslett, Patrick A. J.; Kallas, Esper Georges [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Miami; Albert Einstein Res Inst
Leprosy and human immunodeficiency virus-1 (HIV-1) are examples of human infections where interactions between the pathogen and the host cellular immunity determine the clinical manifestations of disease. Hence, a significant immunopathological interaction between HIV-1 and leprosy might be expected. in the present study we explored several aspects of cellular immunity in patients co-infected with HIV-1 and Mycobacterium leprae. Twenty-eight individuals were studied, comprising four groups: healthy controls, HIV-1 and M. leprae co-infection, HIV-1 mono-infection, and M. leprae mono-infection. Subjects in the mono-infection and co-infection groups were matched as far as possible for bacillary load and HIV disease status, as appropriate. Peripheral blood mononuclear cells (PBMC) were analysed using six- and seven-colour flow cytometry to evaluate T-cell subpopulations and their activation status, dendritic cell (DC) distribution phenotypes and expression of IL-4 by T cells. the co-infected group exhibited lower CD4 : CD8 ratios, higher levels of CD8(+) T-cell activation, increased V delta 1 : V delta 2 T cell ratios and decreased percentages of plasmacytoid DC, compared with HIV-1 mono-infected subjects. Across infected groups, IL-4 production by CD4(+) T lymphocytes was positively correlated with the percentage of effector memory CD4(+) T cells, suggesting antigenically driven differentiation of this population of T cells in both HIV-1 and M. leprae infections. Co-infection with M. leprae may exacerbate the immunopathology of HIV-1 disease. A T helper 2 (Th2) bias in the CD4(+) T-cell response was evident in both HIV-1 infection and leprosy, but no additive effect was apparent in co-infected patients.
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Immunosuppression in undernourished rats: the effect of glutamine supplementation
(Churchill Livingstone, 2003-10-01) Cunha, WDS; Friedler, G.; Vaisberg, Mauro [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Rosa, LFBPC; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
Objective: the aim of our study is to determine the effect of a 30-day-period caloric restriction (CR) upon the immune response of rats and the influence of glutamine upon mononuclear cells proliferation and cytokine production.Methods: Male albino Wistar rats were submitted to CR receiving an amount of food equivalent to 50% of the mean amount consumed by the control animals. We measured the incorporation of [2-C-14] -thymidine by lymphocytes obtained from the spleen and mesenteric lymph nodes, plasma glucose and glutamine concentration, as well as cytokine production by cultivated cells, in the presence of glutamine.Results: Rats submitted to CR presented reduced body weight (49%) and decreased splenic leukocyte number. CR led to a reduction in the proliferative response of lymphocyte. Spleenocytes from CR animals produced less gamma-interferon and interleukins 1, 4 and 10 in 48 h culture than did those from control rats. the same pattern is observed in cells obtained from the mesenteric lymph nodes. the addition of glutamine 2 mM to the culture medium restored spleen and mesenteric lymph node cells' proliferative response and the production of interleukin 2 by cells obtained from the spleen and from the mesenteric lymph nodes.Conclusions: the present data reinforce that undernutrition decreases in vitro immune cell function and indicates that, in such circumstances, glutamine supplementation could reverse some of the changes observed in the functionality of cultured immune cells. the presence of the amino acid at physiological concentration, however, reinforces the diversion of the immune response towards aTh(1)-like response. (C) 2003 Elsevier B.V. All rights reserved.
Acesso aberto (Open Access)
Influência da idade e da doença colorretal na imunocompetência cutânea peri-colostômica
(Cidade Editora Científica Ltda, 2009-12-01) Salles, Valdemir José Alegre; Saad, Sarhan Sydney [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Matos, Delcio [UNIFESP]; Martins, Marcos Roberto; Universidade de Taubaté Departamento de Medicina ASBCP; Universidade Federal de São Paulo (UNIFESP); Universidade de Taubaté Departamento de Medicina
Objective: describe the immunological response in the dermal layer of the peri-colostomic region. Method: Forty-one patients with colostomies realized over eight weeks previously, were included. For the analysis of the immunocellular response in the peri-colostomic dermal region, the values of Pan T lymphocytes, T lymphocytes - helper, T lymphocytes - cytotoxic, lymphocytes B, T lymphocytes - Natural Killer and macrophages. Results: Analysis of the immuno-cellular response showed that both in the benign colorectal disease as well as in the malignant one number of Pan T lymphocytes, T lymphocytes - helper, T lymphocytes - cytotoxic and macrophages were statistically significant relationship major than B Lymphocytes and T lymphocytes - Natural Killer. Analysis of the immuno-cellular response based on age, demonstrated that both the adult age bracket as well as the geriatric one, displayed a major number of Pan T lymphocytes, T lymphocytes - helper and macrophages, with their numerical value significantly than the B lymphocytes and the T lymphocytes - Natural Killer, beyond the T lymphocytes - cytotoxic with the B lymphocytes and the T lymphocytes - Natural Killer in the adult age. Conclusion: The presence of a colostomy promotes the development of an immuno-cellular response in the dermal layer of the peri-colostomy region that is composed of a major number of Pan T lymphocytes, T lymphocytes - helper, T lymphocytes - cytotoxic and macrophages.
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Lymphocytic inflammation in childhood bronchiolitis obliterans
(Wiley-Blackwell, 2004-09-01) Mauad, T.; van Schadewijk, A.; Schrumpf, J.; Hack, C. E.; Fernezlian, S.; Garippo, A. L.; Ejzenberg, B.; Hiemstra, P. S.; Rabe, K. F.; Dolhnikoff, M.; São Paulo BO Study Grp; Universidade de São Paulo (USP); Leiden Univ; Netherlands Red Cross; Free Univ Amsterdam; Universidade Federal de São Paulo (UNIFESP); São Paulo Pediat Assoc
Childhood bronchiolitis obliterans (CBO) is an infrequent, severe disorder characterized by persistent obstructive respiratory symptoms after an acute episode of bronchiolitis. the viral etiology is most common, and adenovirus is the most frequently identified causative agent. Pathologically, the disease is characterized as constrictive type BO, with variable degrees of chronic inflammation and fibrosis in the bronchioles. the nature of the cellular infiltrate is largely unknown, and its characterization may provide better understanding of the disease and offer clues for therapy. Therefore, the aim of the present study was to characterize the inflammatory infiltrate in the bronchioles of 23 open lung biopsies of children with CBO and to compare this to the infiltrate in histologically normal airways. Our results show that CD3+ T cells were the most frequent cell type observed in CBO, with a predominance of the CD8+ T-cell subtype. When compared to the control group, there was a larger number of CD8+, CD4+, CD20+, granzyme B+, and perforin+ lymphocytes in the CBO group. Further studies are needed to address the role of different cell types in the development of CBO.
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Regulatory CD4(+) T lymphocytes after allogeneic transfusion in orthopaedic surgical patients
(Wiley-Blackwell, 2017) Pereira, Luciana de Andrade [UNIFESP]; Baiocchi, Otavio Carvalho Guimarães [UNIFESP]; Silva, Joyce Matie Kinoshita da [UNIFESP]; Takata, Edmilson Takehiro [UNIFESP]; Bordin, Jose Orlando [UNIFESP]
Acesso aberto (Open Access)
A study of T CD4, CD8 and B lymphocytes in narcoleptic patients
(Academia Brasileira de Neurologia - ABNEURO, 2007-06-01) Coelho, Fernando Morgadinho Santos [UNIFESP]; Pradella-Hallinan, Márcia Lurdes de Cássia [UNIFESP]; Alves, Gabriela Rodrigues [UNIFESP]; Bittencourt, Lia Rita Azeredo [UNIFESP]; Pedrazzoli, Mario [UNIFESP]; Moreira, Fábio [UNIFESP]; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Narcolepsy is characterized by excessive daytime sleep and cataplexy. Little is known about the possible difference in pathophysiology between patients with or without cataplexy. OBJECTIVE: To quantify T CD4, T CD8 and B lymphocytes in subgroups of patients with narcolepsy and the presence or absence of the HLA-DQB1*0602 allele between groups. METHOD: Our study was prospective and controlled (transversal) with 22 narcoleptic patients and 23 health control subjects. Patients underwent an all-night polysomnographic recording (PSG) and a multiple sleep latency Test (MSLT). The histocompatibility antigen allele (HLA-DQB1*0602), T CD4, CD8 and B lymphocytes were quantified in control subjects and in narcoleptics. RESULTS: The HLA-DQB1*0602 allele was identified in 10 (62.5%) of our 16 cataplexic subjects and in 2 (33.3%) of the 6 patients without cataplexy (p=0.24). In control subjects, HLA-DQB1*0602 allele was identified in 5 (20%). A significant decrease in T CD4 and B lymphocytes was found in narcoleptic patients with recurrent cataplexy when compared with our patients without cataplexy. CONCLUSION: Autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis were associated with a decrease in sub-group of T CD4 and B lymphocytes. A drop in B lymphocytes count in reumathoid arthritis might, it is posited, be correlated to the presence of HLA-DRB1 allele along with an overall worsened outcome of the affliction. The theory of an increase in consumption of B lymphocytes over the maturation phase has likewise been put forward. Our study reinforces the view that narcolepsy should be considered from an immunological perspective.
Acesso aberto (Open Access)
Vascular endothelial growth factor-A enhances indoleamine 2,3-dioxygenase expression by dendritic cells and subsequently impacts lymphocyte proliferation
(Fundaco Oswaldo Cruz, 2014-02-01) Marti, Luciana Cavalheiro; Pavon, Lorena; Severino, Patricia; Sibov, Tatiana; Guilhen, Daiane; Moreira-Filho, Carlos Alberto; Inst Israelita Ensino & Pesquisa Albert Einstein; Hosp Israelita Albert Einstein; Universidade Federal de São Paulo (UNIFESP)
Dendritic cells (DCs) are antigen (Ag)-presenting cells that activate and stimulate effective immune responses by T cells, but can also act as negative regulators of these responses and thus play important roles in immune regulation. Pro-angiogenic vascular endothelial growth factor (VEGF) has been shown to cause defective DC differentiation and maturation. Previous studies have demonstrated that the addition of VEGF to DC cultures renders these cells weak stimulators of Ag-specific T cells due to the inhibitory effects mediated by VEGF receptor 1 (VEGFR1) and/or VEGFR2 signalling. As the enzyme indoleamine 2,3-dioxygenase (IDO) is recognised as an important negative regulator of immune responses, this study aimed to investigate whether VEGF affects the expression of IDO by DCs and whether VEGF-matured DCs acquire a suppressor phenotype. Our results are the first to demonstrate that VEGF increases the expression and activity of IDO in DCs, which has a suppressive effect on Ag-specific and mitogen-stimulated lymphocyte proliferation. These mechanisms have broad implications for the study of immunological responses and tolerance under conditions as diverse as cancer, graft rejection and autoimmunity.

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