Repositório Institucional - Unifesp :: Navegando por Palavras-chave "immunohistochemistry"

Navegando por Palavras-chave "immunohistochemistry"

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Abnormal expression of MDM2 in prostate carcinoma
(Lippincott Williams & Wilkins, 2001-05-01) Leite, Katia Ramos Moreira [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Srougi, Miguel [UNIFESP]; Nesrallah, Luciano J.; Nesrallah, Adriano; Bevilacqua, Ruy G.; Darini, Elaine; Carvalho, Claudia M.; Meirelles, Maria Ines; Santana, Isaque; Camara-Lopes, L. H.; Hosp Sirio Libanes; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
Mutation of p53 is rare in localized prostate carcinoma the oncoprotein MDM2, whose gene has a response element for p53, promotes the degradation of p53 protein and inhibits its transcriptional activation of genes related to cell cycle arrest and apoptosis, constituting a negative feedback control. We studied p53 and MDM2 expression by immunohistochemistry and looked for mutations in p53 exons 5 to 8 by polymerase chain reaction-single strand conformational polymorphism in 118 patients submitted to radical prostatectomy for localized prostate cancer. in 28 cases, we studied cell proliferation by immunohistochemistry, using antibody for Ki-67, and apoptosis by the deoxynucleotidyl transferase mediated dUTP biotin nick end labeling technique. Although no p53 mutations were found, p53 protein was detected in 31.4% of the cases, and these cases had higher Gleason scores (P = .03) and more advanced tumor stages (P = .02). MDM2 was overexpressed in 40.7% of the cases, and these: cases had greater tumor volumes (P = .001). Tumors that were positive for both p53 and MDM2 were larger (P = .003) and of more advanced stage (P = .03). Within the 28-case subset, the proliferative index was higher among MDM2-positive tumors (P = .046), and the apoptotic index was lower among p53-positive tumors (P = .01). We conclude that, although p53 mutation is a rare event in prostate carcinogenesis, the detection of p53 protein by immunohistochemistry is common and is associated with decreased apoptosis and increased histologic grade and tumor stage. We also conclude that the overexpression of MDM2 has a role in prostate carcinogenesis, being frequently detected and associated with increased cell proliferation and tumor volume. Finally, we propose that the MDM2-positive/53-positive phenotype identifies prostate cancers with aggressive behavior.
Acesso aberto (Open Access)
Adenomas hipofisários: relação entre invasividade e índice proliferativo tumoral
(Academia Brasileira de Neurologia - ABNEURO, 2000-12-01) Tella Jr, Oswaldo Inácio de [UNIFESP]; Herculano, Marco Antonio [UNIFESP]; Delcelo, Rosana [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
We evaluated clinically, radiologically and surgically a series of 76 pituitary adenomas. All cases were assessed immunohistochemically and in 49 patients the PCNA monoclonal antibody was measured. The most frequent types found were the bihormonal adenomas, followed by prolactinomas and non secreting adenomas. The bihormonal adenomas, non secreting adenonas and the sub unit alfa producing adenomas were proportionally more invase as determined by radiological criteria (CTscan or MRI). In 59 patients a transphenoidal approach was used, six cases were operated on transcranially and in 11 patients we used a combination of both approach. Total resection were achieved in 32 cases, most of which were microadenomas, in 15 cases the resection was subtotal and partial in 29 cases. Diabetes insipidus was the most frequent endocrine complication. It was observed that secreting adenomas tend to be associated with an increased PCNA and invasive adenomas correlated with PCNA 3 and 4. An improvement in vision was observed in 85% of macroadenomas seen after a total, subtotal or partial resection.
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Altered Balance of gamma-Aminobutyric Acidergic and Glutamatergic Afferent Inputs in Rostral Ventrolateral Medulla-Projecting Neurons in the Paraventricular Nucleus of the Hypothalamus of Renovascular Hypertensive Rats
(Wiley-Blackwell, 2010-03-01) Biancardi, Vinicia Campana; Campos, Ruy Ribeiro [UNIFESP]; Stern, Javier Eduardo; Med Coll Georgia; Universidade Federal de São Paulo (UNIFESP)
An imbalance of excitatory and inhibitory functions has been shown to contribute to numerous pathological disorders. Accumulating evidence supports the idea that a change in hypothalamic gamma-aminobutyric acid (GABA)-ergic inhibitory and glutamatergic excitatory synaptic functions contributes to exacerbated neurohumoral drive in prevalent cardiovascular disorders, including hypertension. However, the precise underlying mechanisms and neuronal substrates are still not fully elucidated in the present study, we combined quantitative immunohistochemistry with neuronal tract tracing to determine whether plastic remodeling of afferent GABAergic and glutamatergic inputs into identified RVLM-projecting neurons of the hypothalamic paraventricular nucleus (PVN-RVLM) contributes to an imbalanced excitatory/inhibitory function in renovascular hypertensive rats (RVH). Our results indicate that both GABAergic and glutamatergic innervation densities increased in oxytocin-positive, PVN-RVLM (OT-PVN-RVLM) neurons in RVH rats. Despite this concomitant increase, time-dependent and compartment-specific differences in the reorganization of these inputs resulted in an altered balance of excitatory/inhibitory inputs in somatic and dendritic compartments. A net predominance of excitatory over inhibitory inputs was found in OT-PVN-RVLM proximal dendrites. Our results indicate that, along with previously described changes in neurotransmitter release probability and postsynaptic receptor function, remodeling of GABAergic and glutamatergic afferent inputs contributes as an underlying mechanism to the altered excitatory/inhibitory balance in the PVN of hypertensive rats. J. Comp. Neurol. 518:567-585, 2010. (C) 2009 Wiley-Liss, Inc
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Análise da expressão de moléculas de adesão celular nos linfomas de células t cutâneos e em doenças inflamatórias da pele
(Universidade Federal de São Paulo (UNIFESP), 2016-11-23) Barata, Mariangela Cristina Crispino [UNIFESP]; Silva, Maria Regina Regis Silva [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
To investigate possible differences in cell adhesion molecules expression between mycosis fungoides (MF) and inflammatory skin diseases (drug reactions and allergic contact dermatitis). Patients and Methods: We selected 33 biopsies from patients with mycosis fungoides and 10 biopsies of patients with inflammatory skin diseases from Departamento de Patologia da Universidade Federal de São Paulo (UNIFESP) from January 1997 to December 2013. Expression of ?4?1 integrin, ?E?7 integrin and E-cadherin was assessed by immunohistochemistry in intraepidermal lymphocytes by counting four high power microscopic epidermal fields (400x) and comparing those between the two groups. Results: There was no difference in the expression of integrins ?4?1 and ?E?7 in intraepidermal lymphocytes between cases of MF and inflammatory skin diseases. We observed increased expression of integrin ?E?7 in intraepidermal lymphocytes in advanced stages of MF (T3 and T4) and in cases of MF after treatment. The expression of E-cadherin in epidermal cells in MF outlined Pautrier microabscesses, whereas in inflammatory diseases, spongiosis and intercelular oedema reduced it?s expression in keratinocytes. Conclusion: The increased expression of integrin ?E?7 in advanced stages of MF (T3 and T4) can occur due to increased lymphocyte population or the presence of lymphocytes that also express that integrin, although it may not necessarily be activated. Increased expression of ?E?7 in intraepidermal lymphocytes of posttreatment MF cases may be related to the increase of non-tumoral infiltrating lymphocytes in the epidermis. The expression of E-cadherin in the epidermis in cases of MF outlined Pautrier microabscesses, unlike inflammatory diseases, in which there is swelling, breaking of intercellular bridges and decreased expression of this molecule, showing that tumor lymphocytes may migrate to the epithelium in a less aggressive manner, respecting the structure of keratinocytes.
Acesso aberto (Open Access)
Análise da expressão do colágeno VI na distrofia muscular congênita
(Academia Brasileira de Neurologia - ABNEURO, 2005-06-01) Freitas, Regina Toni Loureiro de [UNIFESP]; Zanoteli, Edmar [UNIFESP]; Morita, Maria Da Penha Ananias [UNIFESP]; Oliveira, Acary Souza Bulle [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Congenital muscular dystrophy (CMD) composes a group of disorders characterized by hypotonia and muscular weakness noticed in the first year of life. The Ullrich's form is characterized by proximal joint contractures and distal hiperextensibility. About 40% of these patients present mutations in one of the genes that codify the sub-units of the collagen VI protein (COL6), producing total or partial deficiency of the protein expression. We analyzed, through immunohistochemistry, the expression of COL6 in muscle fragments of 50 patients with CMD; 20 of them presented merosin expression deficiency. We identified 4 cases with total COL6 deficiency (8% of the total), representing 13% of the cases with normal merosin expression. The histological findings of patients with deficiency of COL6 were indistinguishable from other forms of CMD, but milder than that abnormalities observed in merosin deficient patients. In three COL6 deficient patients were observed hypotonia and weakness in the neonatal period, delayed of motor milestones, muscular retractions of knees and elbows, distal joint hiperextensibility and congenital hip dislocation (two patients). One patient lost the ability to walk; and one died due to respiratory problems. The analysis of COL6 expression, as well as merosin expression, in the muscle tissue from CMD patients, can be important for identification and phenotypic characterization of different CMD subtypes.
Acesso aberto (Open Access)
Anatomopathological characterization of placentas from HIV+ patients associated with p24 expression
(Sociedade Brasileira de Patologia ClínicaSociedade Brasileira de PatologiaSociedade Brasileira de Citopatologia, 2013-12-01) López, Consuelo Lozoya; Pires, Andréa Rodrigues Cordovil; Fonseca, Eliene Carvalho De; Rodrigues, Fabiana Resende; Braga Neto, Antônio Rodrigues; Herdy, Gesmar Volga Haddad; Vasques, Flávio Augusto Prado [UNIFESP]; Lopes, Vania Gloria Silami; Universidade Federal Fluminense; UFF Pathology; Fundação Oswaldo Cruz Cellular and Molecular Biology; Universidade Estadual Paulista (UNESP); Universidade Federal do Rio de Janeiro Cardiology; Universidade Federal de São Paulo (UNIFESP)
INTRODUCTION: The study of placentas from pregnant human immunodeficiency virus (HIV) positive women has become the subject of numerous studies in the literature. Morphological, viral, immune and inflammatory placental aspects have been analyzed in order to grasp the vertical transmission of the virus. OBJECTIVE: To identify the most frequent findings in the placentas by associating them with a viral antigen and correlating them with the infection of newborns. MATERIAL AND METHODS: Thirty-five placentas from HIV- positive pregnant women were pathologically and immunohistochemically analyzed with the use of p24 antibody in the period from 1992 to1997 in accordance with the routine laboratory testing from the Anatomopathological Department - Hospital Universitário Antônio Pedro - Universidade Federal Fluminense (APD/HUAP/UFF). RESULTS: The microscopic alterations detected in all cases, including those with vertical transmission, were arteriopathy in the fetal blood circulation, chorioamnionitis, perivillous fibrin deposition, syncytial knotting, villous edema and villous immaturity. No specific macroscopic or histopathological changes were found in these placentas. The neonatal infection was observed in five cases. Vertical transmission was identified in two out of five placentas that had low weight for the respective stage of pregnancy. Immunohistochemical analysis revealed 14 positive cases, two of which showed vertical transmission. The viral protein was not identified in 10 out of 14 placentas from patients who had been medicated with zidovudine (AZT). CONCLUSION: Our study has contributed to the anatomopathological investigation into placentas from HIV-positive patients, although p24 expression per se did not allow a definite and early diagnosis of the vertical transmission.
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Angiogenesis and skin carcinomas with skull base invasion: A case-control study
(Wiley-Blackwell, 2004-05-01) Cernea, C. R.; Ferraz, A. R.; Castro, IV de; Sotto, M. N.; Logullo, A. F.; Bacchi, C. E.; Potenza, A. S.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
Background. Some skin carcinomas may be very aggressive. Intensity of angiogenesis, measured by intratumoral vessel density using expression of CD34, has been associated with tumor aggressiveness. in this study, the expression of CD34 in basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs) with skull base invasion was compared with that in tumors with good outcome.Methods. Expression of CD34 was graded as mild, moderate, and intense, in 24 BCCs and 11 SCCs with skull base invasion. the control group included 23 BCCs and 10 SCCs.Results. Intense expression of CD34 was noted in 25.00% of BCCs with skull base invasion, compared with 4.35% in the control group (p =.058). Regarding SCCs, intense expression of CD34 was found in 54.55% of aggressive tumors, compared with 10.00% in the control group (p = 133).Conclusions. A trend toward denser microvascular angiogenesis was observed in both BCCs and SCCs with skull base invasion compared with less aggressive controls. (C) 2004 Wiley Periodicals, Inc.
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Antigen retrieval methods and estrogen receptor immunoexpression using ID5 antibody: A comparative study
(Westminster Publ Inc, 2005-10-01) Neves, J. I.; Begnami, M. D.; Arias, V; Santos, G. C.; AC Camargo Hosp; Samaritano Hosp; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
Long-term formalin tissue fixation results in antigen masking, probably through aldehydic linkage between proteins and fixative molecules. Immunohistochemistry results depend on the type of the detection procedure and the type of antibody used for the reaction. Considering the difficulty in working with estrogen receptor (ER) antibodies and the lack of standardization of the antigen retrieval methods, we quantified the immunoexpression of ER using the ID5 antibody and a standard streptavidin-biotin detection procedure retrieving with microwave even, steamer, pressure cooker, and water bath in a set of SBR grade 2 invasive breast carcinomas. Pressure-cooking provided the best results. No significant differences were observed in using the other methods. Pressure-cooking should be recommended as the method of choice for standardization of the ER immuohistochemical reaction.
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Avaliação do anticorpo monoclonal g12f2 para reação de imuno-histoquímica do melanoma murino b16f10
(Universidade Federal de São Paulo (UNIFESP), 2015-02-25) Grillo, Carlos Alberto [UNIFESP]; Lopes, Jose Daniel Lopes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
In 2006, at the Department of Microbiology, Immunology and Parasitology of Federal University of São Paulo - UNIFESP, was developed a new monoclonal antibody (mAb), type IgM, designated G12F2, which recognized a single band of approximately 230 kDa in total protein extract of melanoma murine B16F10. Interactions between antigenantibody represent the reaction principle of immunohistochemistry (IHC) performed in the laboratory. Therefore, the capacity of monoclonal antibody G12F2 to recognize a molecule of murine melanoma B16F10 can help the study and diagnosis of murine melanoma by IHC reaction. Objectives: Evaluation of the efficiency of monoclonal antibody G12F2 in the IHC reaction to identify the melanoma murine B16F10. Materials and Methods: The monoclonal antibody G12F2 was studied by IHC reaction in 02 groups of female mice C57BL6. Initially, B16F10 cells were maintained in culture and used to induce the melanoma in vivo. A group of five mice received subcutaneous injection of B16F10 cells in the left flank, in order to induce subcutaneous nodule. Another group of five mice received intravenous injection of B16F10 cells in the tail, in order to induce pulmonary metastases. After 15 to 21 days, skin and pulmonary tissues containing tumor nodules, as well as lymph node with tumor cells were collected for IHC analysis. Samples were collected, fixed and forwarded to the pathology laboratory. Blocks of paraffin containing the samples were stained by hematoxylin and eosin (HE) and after evaluated by IHC. Immunohistochemical reactions were performed in accordance with procedures previously established at the Department of Pathology for use of the monoclonal antibody G12F2. Results: All mice (n = 10) developed melanoma after injection of B16F10 cells; which 5 mice developed subcutaneous nodule in the left flank and 5 mice developed pulmonary nodules. Also was collected a subcutaneous lymph node with metastasis. After the procedures of fixation, preparation of the paraffin block and stained with HE, slides containing samples of nodules were used in the IHC reaction using monoclonal antibody G12F2. Positive reaction was observed by brown staining in the cell membrane of tumor cells present in subcutaneous and pulmonary nodules and lymph node containing B16F10 cells. However, monoclonal antibody G12F2 also labeled other non-tumor tissues as: normal alveolar epithelium, subcutaneous and adipose tissues and the secretory portion of the sebaceous gland. There was no labeling in lymphoid tissue, blood vessels wall, bronchi, melanocytes or other components of the epidermis and basal membrane. This fact shows that the cell membrane protein recognized by the monoclonal antibody G12F2 in B16F10 cells, is also present on the cell membrane of non-tumor tissues. Conclusion: Due to the fact of monoclonal antibody G12F2 recognize an antigen present in the cell membrane not only of B16F10 murine melanoma cells, but also in pulmonary alveoli, adipocytes and sebaceous glands, it is possible to conclude that this antibody is not a melanoma specific marker and as recommended for using with other tumor markers, both positive labeling and morphological analysis are necessary to definitive diagnosis of melanoma
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Avaliação histomorfométrica e imunoistoquímica do uso da hidroxiapatia manométrica em defeitos ósseos na calota craniana de ratos
(Universidade Federal de São Paulo (UNIFESP), 2016-06-29) Faria, Marcio Antonio [UNIFESP]; Simoes, Manuel de Jesus Simoes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Currently it is considered a challenge to surgical reconstruction of bone defects created by trauma and degenerative diseases. It is eventually necessary to use autogenous bone or other bone substitute biomaterials, performing procedures grafts and transplants. As part of these biomaterials, contains the hydroxyapatites (HA), which have shown promising for behaving biocompatible way. However, it is known that the size of the HA particles is important in bone healing process, once the biomaterial should be reabsorbed to make room for the bone tissue. In this context, the hydroxyapatite nanometer (nHA) has a promising role in the healing process of the bone tissue because of their small size and approach the size of the natural HA of bone crystals. The objective of this study was to evaluate the effect of nHA paste (ReproBone ®) in bone defects created in the skull of Wistar rats after 7, 14 and 21 days, by histomorphometric and immunohistochemical analysis for VEGFA. The nHA was implanted in calvarial defects in the test group while the control group defects were filled only with blood clot. The animals were divided into 6 groups: GI (ctrl 7 days), GII (ctrl 14 days), GIII (ctrl 21 days), GIV (test 7 days), GV (trial 14 days), GVI (test 21 days). After surgery, the animals were euthanized and the calvaria removed for histological and immunohistochemical processing. The results showed that the test groups increased formation of bone tissue compared to control groups at 21 days was the total closure of the bone defect in the test group. There was a greater reactivity to Vegf in connective tissue region and the periphery of the newly formed bone in the test groups compared to control groups. It follows from the data obtained that nHA accelerates bone regeneration process at all times 7, 14 and 21 days and that its use could promote full healing of a critical defect in bone tissue.
Acesso aberto (Open Access)
C-kit expression in human osteosarcoma and in vitro assays
(E-century Publishing Corp, 2011-01-01) Miiji, Luciana Nakao Odashiro [UNIFESP]; Petrilli, Antonio Sergio [UNIFESP]; Di Cesare, Sebastian; Odashiro, Alexandre Nakao [UNIFESP]; Burnier, Miguel Noel Nascente [UNIFESP]; Toledo, Silvia Regina Caminada de [UNIFESP]; Garcia, Reynaldo Jesus; Alves, Maria Teresa de Seixas [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); McGill Univ; Ctr Hosp Afillie Univ Quebec
Biologic agents targeting oncogenes have encourage researchs trying to correlate the role of tyrosine kinase in the pathogenesis of tumours. Osteosarcoma is a high grade aggressive neoplasm with poor survival. Our aim was to investigate c-kit immunoexpression, its prognostic relevance for patients with osteosarcoma, and the effect of imatinib mesylate (STI571) on proliferation and invasion of the human osteosarcoma cell line. A retrospective immunohistochemical study was performed on archival formalin-fixed paraffin-embedded specimens from 52 patients with high-grade primary osteosarcoma of extremities treated at the Pediatric Oncology Institute (IOP, GRAAC) and archived in the Department of Pathology, Federal University of Sao Paulo. Only pre-chemotherapy specimens were analyzed. Strongly stained cytoplasm and membrane cells were taken as positive. Human osteosarcoma cells from line MG-63 were incubated and the inhibitory effect of imatinib mesylate (STI571) on cell proliferation and invasion was studied. In 24 cases (46.15%), c-kit was expressed by the cells and c-kit-positive tumors exhibited lower necrosis post-chemotherapy. No correlation was found between c-kit expression and overall and disease-free survival. Imatinib mesylate decreased the rates of cell growth of osteosarcoma cells in low doses and invasion in high doses C-kit-positive tumors had worse response to chemotherapy and imatinib mesylate can play a role in blocking or decreasing the rate of growth of osteosarcoma cells, but not the invasive capacity of these neoplastic cells. These data suggested that imatinib mesylate could be a therapeutic target of strategies against osteosarcoma tumors. Further studies are necessary to confirm this indication.
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Cimetidine Reduces Interleukin-6, Matrix Metalloproteinases-1 and-9 Immunoexpression in the Gingival Mucosa of Rat Molars With Induced Periodontal Disease
(Amer Acad Periodontology, 2017) Oliveira, Priscila Aparecida de [UNIFESP]; Pizzol Junior, Jose Paulo de [UNIFESP]; Longhini, Renata [UNIFESP; Sasso-Cerri, Estela; Cerri, Paulo Sergio
Background: Histamine seems to act, via H-2 receptor, on inflammatory processes by stimulating interleukin (IL)-6 and matrix metalloproteinase (MMP) release. As cimetidine is an H-2 receptor antagonist, the authors hypothesize that this antiulcer drug reduces IL-6, MMP-1, andMMP-9immunoexpressioningingiva with inducedperiodontal disease (PD). Toconfirmapossible modulatory role of IL-6 on MMPs, the relationship between IL-6/MMP-1 and IL-6/MMP-9 immunoexpression was evaluated. Methods: Forty-six male rats were distributed into the cimetidine group (CimG: received daily intraperitoneal injections of 100 mg/kg of body weight of cimetidine) or the saline group (SG). PD was induced by cotton ligature around the maxillary left first molars (PDSG and PDCimG). The right molars were used as controls (SG and CimG). After 7, 15, 30, and 50 days, maxillary fragments were processed for paraffin embedding or for transmission electron microscopy. Tartrateresistant acid phosphatase (TRAP)-positive osteoclasts in the alveolar process surface and number of IL-6, MMP-1, and MMP-9-immunolabeled cells in the gingival mucosa were quantified. Statistical analyses were performed (P <= 0.05). Results: In PDSG and PDCimG, gingival mucosa exhibited few collagen fibers among numerous inflammatory cells. In PDCimG, the number of TRAP-positive osteoclasts and IL-6, MMP-1, andMMP-9-immunolabeled cells was significantly lower than in PDSG at all periods. A positive correlation between IL-6/MMP-1 and IL-6/MMP-9 was detected in PDSG and PDCimG. Conclusion: Cimetidine decreases bone loss through reduction of osteoclast number and induces reduction of IL-6, MMP-1, and MMP-9 immunoexpression, reinforcing the idea that the beneficial effect of cimetidine in PD may be due to reduction of IL-6 immunolabeling in the inflamed gingival mucosa.
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Coexpression of cytokeratin and vimentin in mice trophoblastic giant cells
(Churchill Livingstone, 2001-02-01) Souza, P. C. de; Katz, S. G.; Universidade Federal de São Paulo (UNIFESP)
Trophoblastic giant cells reach their maximum size and exhibit a conspicuous synthetic and invasive activity during mouse placentation, the cytoskeleton, given the complex functions of the cells, shows a well-developed network of intermediate filament proteins. Immunohistochemistry combined with confocal and conventional immunofluorescence studies of intermediate filaments proteins cytokeratin and vimentin were performed in mice trophoblastic giant cells on days 9-11 of pregnancy. Specimens were fixed in phosphate-buffered formaldehyde and tissues were processed for routine paraffin embedding. Trophoblastic giant cells from antimesometrial, lateral or mesometrial uterine regions, through days 9-11 of pregnancy, expressed the same staining with both immunoperoxidase and immunofluorescent techniques. Cytokeratin filamentous structures were intensely immunoreactive and were detected throughout the cells cytoplasm; a few cells exhibited strongest fluorescence in the peripheral cytoplasm, Vimentin-positive staining was often distributed throughout the cells cytoplasm, most frequently and more intensely in the peripheral region; in some cells, it was present only in the peripheral regions. It is probable that expression of vimentin in midpregnancy trophoblastic giant cells may be associated with the rapid and conspicuous increase in size and synthetic activity of the cells and also with phagocytosis of degraded materials and invasion of decidual tissue. (C) 2001 Harcourt Publishers Ltd.
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Comparison of EphA receptor tyrosine kinases and ephrinA ligand expression to EphB-ephrinB in vascularized corneas
(Lippincott Williams & Wilkins, 2007-06-01) Kojima, Takashi; Chung, Tae-Young; Chang, Jin-Hong; Sayegh, Rony; Casanova, Fábio Henrique Cacho [UNIFESP]; Azar, Dimitri T.; Univ Illinois; Sungkyunkwan Univ; Harvard Univ; Universidade Federal de São Paulo (UNIFESP)
Purpose: Eph cell surface receptors and their ligands, ephrins, are involved in neuronal patterning and neovascularization. Our purpose is to compare and characterize the expression of ephrinA ligands and EphA receptors to ephrinB; ligands and EphB receptors in excised mouse corneal tissue, in corneal epithelial and keratocyte cell lines, and during corneal angiogenesis.Methods: Mouse corneal epithelial cells and keratocytes were immortalized using SV40T antigen viral infection of primary cultures. The immortalized epithelial cells and keratocytes were cloned and characterized using antibodies to keratin, vimentin, integrin alpha 5 beta 1, and alpha-smooth muscle actin. Basic fibroblast growth factor pellets were implanted to induce corneal neovascularization. The eyes of wild-type, ephrinB2(tlacZ/+), and EphB4(tlacZ/+) heterozygous mice were harvested and sectioned 7 days after pellet implantation. Confocal immunohistochemistry was performed to compare the expression of the Eph/ephrinA family (EphA1-8, ephrinA1-5) and Eph/ephrinB family (EphB1-4, EphB6 ephrinB1-3).Results: EphA1, EphA3, ephrinA1, ephrinA2, EphB1, EphB4, ephrinB1, and ephrinB2 were detected in wild-type mouse corneal epithelial cells and keratocytes. EphA2 was immunolocalized only in epithelial cells. Also, EphA3, ephrinA1, EphB1, EphB4, and ephrinB1 were immunolocalized to the comeal epithelium and stroma. In the vascularized corneas, ephrinB1 was immunolocalized mainly to the keratocytes around the vessels, and ephrinB2, EphB 1, and EphB4 were colocalized mainly with CD31 to the vascular endothelial cells.Conclusions: The characterization of ephrin ligand and Eph receptor expression during cornea angiogensis in this study suggests that the Eph/ephrin family of receptor tyrosine kinases and their ligands may play a role in the regulation of corneal angiogenesis.
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Decorin and biglycan immunolocalization in non-villous structures of healthy and pathological human placentas
(Wiley-Blackwell, 2014-04-01) Borbely, Alexandre Urban; Daher, Silvia [UNIFESP]; Ishigai, Marcia Marcelino de Souza. [UNIFESP]; Mattar, Rosiane [UNIFESP]; Sun, Sue Yazaki [UNIFESP]; Knoefler, Martin; Bevilacqua, Estela Maris Andrade Forell; Oliveira, Sergio Ferreira; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Med Univ Vienna
AimsDecorin and biglycan are members of the small leucine-rich proteoglycan family, and constituents of both the extracellular matrix (ECM) and the cell surface. They are recognized as important factors in the control of proliferation, migration and invasion invivo and invitro. in this study, the localization patterns of decorin and biglycan were determined in healthy placentas and in highly invasive placental pathologies.Methods and resultsThe study included immunolocalization of decorin and biglycan in samples of first-trimester and term placentas, placenta accreta, invasive mole, and choriocarcinoma. Extravillous cytotrophoblast (EVT) cells were positive for both proteoglycans in all pathologies and in first-trimester placentas, although not in term placentas. Biglycan was immunolocalized in the ECM of all healthy and pathological placentas, whereas decorin was observed only in term placenta ECM.ConclusionsThe expression of both proteoglycans was cell-specific and gestation time-dependent in healthy placentas, and was associated with invasive EVT cells in pathological placentas. in view of the biological properties of these molecules, it is possible that the biglycan pattern found here is intrinsically implicated in the invasive activity of EVT cells in both healthy and disordered placentas.
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Development of granulocytes in haematopoietic tissues of Rhizoprionodon lalandii
(Elsevier B.V., 2002-10-01) Pacheco, F. J.; Pacheco, SOS; Segreto, HRC; Segreto, R. A.; Silva, MRR; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Granulocytes of the epigonal and Leydig organs of Rhizoprionodon lalandii were identified and classified into three different cell types, type I and type II eosinophils and neutrophils. the development of these cells in the haematopoietic tissues was dynamic, demonstrated by nuclear immunopositivity for the proliferating cell nuclear antigen (PCNA) proteins and was regulated by various cytokines, including the transforming growth factor beta-1 (TGFbeta(1)). the expression pattern of these cells was heterogeneous among individual cells and TGFbeta(1)-immunostaining was found principally in the cytoplasm of immature granulocytes. the presence of TGFbeta(1) in cells about to divide was demonstrated suggesting that modulation of differentiation and proliferation occurs in the haematopoietic tissues of this species of elasmobranch. (C) 2002 the Fisheries Society of the British Isles. Published by Elsevier B.V. All rights reserved.
Acesso aberto (Open Access)
Diferenciação histopatológica e imunoistoquímica das alterações epiteliais no nódulo vocal em relação aos pólipos e ao edema de laringe
(ABORL-CCF Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial, 2004-08-01) Neves, Beatrice M. J. [UNIFESP]; G. Neto, João; Pontes, Paulo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
AIM: To evaluate by histological and immunohistochemical methods the epithelial lesions in vocal nodule and correlate with polyp, laryngeal edema and vocal folds without macroscopic lesions. STUDY DESIGN: Chart review. MATERIAL AND METHOD: In a retrospective analysis of medical records, twenty-six patients, who underwent microsurgical excision of laryngeal inflammatory lesions (nodules, polyps and laryngeal edema), were identified. Vocal folds without macroscopic lesions were obtained from autopsy. To evaluate epithelial lesions, specimens were stained with H&E, PAS, and with antibodies against laminin and collagen type IV. These specimens were analyzed by light microscopy. RESULTS: Lesions diagnosed as nodules showed more collagen type IV and laminin deposition when compared to polyps (p=0,034 and p=0,036, respectively) and to vocal folds without macroscopic lesions (p=0,019 and p=0,021, respectively). Nodules showed basement membranes thicker than polyps on PAS stain (p=0,102). Laryngeal edema could not be differentiated from nodules, polyps or vocal folds without macroscopic lesions by PAS, laminin or collagen type IV stain (p>0,10). CONCLUSION: By histological and immunohistochemical techniques to evaluate epithelium of laryngeal inflammatory lesions we conclude that vocal fold nodule differentiate from polyps on the three techniques used to stain basement membrane (PAS, laminin, collagen IV) and from vocal folds without macroscopic lesions on two techniques used (laminin, collagen IV). Laryngeal edema cannot be differentiated from the others laryngeal lesions, neither from vocal folds without macroscopic lesions, when the techniques before described are used.
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Early nuclear alterations and immunohistochemical expression of Ki-67, Erb-B2, vascular endothelial growth factor (VEGF), transforming growth factor (TGF-BETA 1) and integrine-linked kinase (ILK) two days after tamoxifen in breast carcinoma
(Veda, Slovak Academy Sciences, 2004-01-01) Morena, Ana Maria Lira [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Gebrim, Luiz Henrique [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Silva, Maria Regina Regis da [UNIFESP]; Segreto, Roberto Araujo [UNIFESP]; Giannotti Filho, Osvaldo [UNIFESP]; Teixeira, Vicente de Paulo Castro [UNIFESP]; Segreto, Helena Regina Comodo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Oncoctr Fdn Sao Paulo
The purpose of the present study was to evaluate breast carcinoma samples before and two days after treatment with tamoxifen in order to analyse early histopathological alterations - particularly nuclear alterations - as well as immunohistochemical expression of Ki-67, Erb-B2, VEGF, TGf-beta 1 and ILK proteins. Twenty one cases of invasive ductal and lobular breast carcinoma were studied. Patients were submitted to biopsy of the lesion and, after confirmation of the diagnosis, they received 20 mg of tamoxifen a day, beginning two days before surgery. The samples obtained during biopsy and after surgery were stained with HE for histopathological diagnosis. Estrogen receptor was positive in IS cases and negative in 3. The immunohistochemical method was applied for the detection of Ki-67, Erb-B2, protein, vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-beta 1) and integrin linked kinase (ILK).Two days after tamoxifen treatment, the following results were observed: 1) decrease in the cell volume, chomatine condensation, nucleoli less evident and clearly defined nuclear limits; 2) significant reduction in the expression of Erb-B2 protein and significant increase in the expression of TGF-beta 1 protein; 3) expression of others proteins (Ki-67, VEGF and ILK) was not altered during the indicated time frame.Our results suggest that analyzing nuclear alterations and expression of Erb-B2 and TGF-beta 1 proteins would be useful to assess the initial response to tamoxifen.
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Expression of SIRT1 in Ocular Surface Squamous Neoplasia
(Lippincott Williams & Wilkins, 2012-07-01) Alves, Luiz Filipe de A. [UNIFESP]; Fernandes, Bruno F.; Burnier, Julia V.; Mansure, Jose J.; Maloney, Shawn; Odashiro, Alexandre N.; Antecka, Emilia; De Souza, Dominique F.; Burnier, Miguel N.; Henry C Witelson Ocular Pathol Lab; Brazilian AF Cent Hosp; Universidade Federal de São Paulo (UNIFESP); McGill Univ
Background: the class III histone deacetylase SIRT1 is overexpressed in many malignancies and has been implicated in inactivating proteins that are involved in tumor suppression and DNA damage repair. in the current study, we examined the expression of SIRT1 in normal epithelium (NE) compared with ocular surface squamous neoplasia (OSSN) to elucidate a possible role for SIRT1 in the development or progression of this malignancy.Methods: We examined SIRT1 expression by immunohistochemistry in 47 cases of OSSN and 10 specimens of NE. Our sample included 11 benign lesions (papillomas), 25 cases of conjunctival intraepithelial neoplasia, and 11 malignant lesions of squamous cell carcinoma. the extent of staining and intensity was scored and the combined raw data were then converted to the German Immunoreactive Score.Results: Nuclear and cytoplasmic expression of SIRT1 was observed in all cases of OSSN. for the NE specimens, 50% showed negative expression and 30% weak expression, and 20% were considered significantly immunoreactive. the differential expression of SIRT1 between NE and OSSN was statistically significant (P < 0.0001). Additionally, when the staining pattern in cases of conjunctival intraepithelial neoplasia was evaluated, the staining of the more differentiated surface cells was remarkably weaker compared with the cells located closer to the basal membrane.Conclusions: SIRT1 may play an important role in the development and progression of epithelial tumors of the conjunctiva. Further research into the potential of SIRT1 as a novel therapeutic target is warranted.
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Expression Profiling of Cell Cycle Regulatory Proteins in Oropharyngeal Carcinomas Using Tissue Microarrays
(Int Inst Anticancer Research, 2010-05-01) Ribeiro, Daniel Araki [UNIFESP]; Nascimento, Fábio Dupart [UNIFESP]; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Gomes, Thiago Simao [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Franco, Marcello Fabiano de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Aim: The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis. Patients and Methods: Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays. Results: Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx. Conclusion: Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.

Navegando por Palavras-chave "immunohistochemistry"

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