Navegando por Palavras-chave "histomorphometry"
- ItemSomente MetadadadosClinical and histomorphometric evaluation of extraction sockets treated with an autologous bone marrow graft(Wiley-Blackwell, 2010-05-01) Pelegrine, Andre Antonio; Sorgi da Costa, Carlos Eduardo [UNIFESP]; Pizzigatti Correa, Maria Elvira; Comenalli Marques, Jose Francisco; Sao Leopoldo Mand Res Ctr; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP)PurposeThe aim of this study was to evaluate the potential of an autologous bone marrow graft in preserving the alveolar ridges following tooth extraction.MaterialsThirteen patients requiring extractions of 30 upper anterior teeth were enrolled in this study. They were randomized into two groups: seven patients with 15 teeth to be extracted in the test group and six patients with 15 teeth to be extracted in the control group. Hematologists collected 5 ml of bone marrow from the iliac crest of the patients in the test group immediately before the extractions. Following tooth extraction and elevation of a buccal full-thickness flap, titanium screws were positioned throughout the buccal to the lingual plate and were used as reference points for measurement purposes. the sockets were grafted with an autologous bone marrow in the test sites and nothing was grafted in the control sites. After 6 months, the sites were re-opened and bone loss measurements for thickness and height were taken. Additionally, before implant placement, bone cores were harvested and prepared for histologic and histomorphometric evaluation.ResultsThe test group showed better results (P < 0.05) in preserving alveolar ridges for thickness, with 1.14 +/- 0.87 mm (median 1) of bone loss, compared with the control group, which had 2.46 +/- 0.4 mm (median 2.5) of bone loss. the height of bone loss on the buccal plate was also greater in the control group than in the test group (P < 0.05), 1.17 +/- 0.26 mm (median 1) and 0.62+0.51 (median 0.5), respectively. in five locations in the control group, expansion or bone grafting complementary procedures were required to install implants while these procedures were not required for any of the locations in the test group. the histomorphometric analysis showed similar amounts of mineralized bone in both the control and the test groups, 42.87 +/- 11.33% (median 43.75%) and 45.47 +/- 7.21% (median 45%), respectively.ConclusionThese findings suggest that the autologous bone marrow graft can contribute to alveolar bone repair after tooth extraction.To cite this article:Pelegrine AA, da Costa CES, Correa MEP, Marques JFC Jr. Clinical and histomorphometric evaluation of extraction sockets treated with an autologous bone marrow graft.Clin. Oral Impl. Res. 21, 2010; 535-542.doi: 10.1111/j.1600-0501.2009.01891.x.
- ItemSomente MetadadadosEnhancement of sciatic nerve regeneration after vascular endothelial growth factor (VEGF) gene therapy(Wiley-Blackwell, 2011-10-01) Pereira Lopes, F. R.; Lisboa, B. C. G. [UNIFESP]; Frattini, F.; Almeida, F. M.; Tomaz, M. A.; Matsumoto, P. K. [UNIFESP]; Langone, F.; Lora, S.; Melo, P. A.; Borojevic, R.; Han, S. W. [UNIFESP]; Martinez, A. M. B.; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Campinas (UNICAMP); Ist Nazl Fis NuclAims: Recent studies have emphasized the beneficial effects of the vascular endothelial growth factor (VEGF) on neurone survival and Schwann cell proliferation. VEGF is a potent angiogenic factor, and angiogenesis has long been recognized as an important and necessary step during tissue repair. Here, we investigated the effects of VEGF on sciatic nerve regeneration. Methods: Using light and electron microscopy, we evaluated sciatic nerve regeneration after transection and VEGF gene therapy. We examined the survival of the neurones in the dorsal root ganglia and in lumbar 4 segment of spinal cord. We also evaluated the functional recovery using the sciatic functional index and gastrocnemius muscle weight. in addition, we evaluated the VEGF expression by immunohistochemistry. Results: Fluorescein isothiocyanate-dextran (FITC-dextran) fluorescence of nerves and muscles revealed intense staining in the VEGF-treated group. Quantitative analysis showed that the numbers of myelinated fibres and blood vessels were significantly higher in VEGF-treated animals. VEGF also increased the survival of neurone cell bodies in dorsal root ganglia and in spinal cord. the sciatic functional index and gastrocnemius muscle weight reached significantly higher values in VEGF-treated animals. Conclusion: We demonstrate a positive relationship between increased vascularization and enhanced nerve regeneration, indicating that VEGF administration can support and enhance the growth of regenerating nerve fibres, probably through a combination of angiogenic, neurotrophic and neuroprotective effects.
- ItemSomente MetadadadosHistologic and histomorphometric evaluation of a synthetic bone substitute for maxillary sinus grafting in humans(Wiley-Blackwell, 2013-08-01) Tosta, Mauro; Cortes, Arthur R. G.; Correa, Luciana; Pinto, Decio dos S.; Tumenas, Isabel [UNIFESP]; Katchburian, Eduardo [UNIFESP]; CETAO; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Objective: the present study investigated the efficacy of a biphasic calcium phosphate as a bone grafting material for maxillary sinus augmentation in humans.Materials and methods: Half of the thirty patients selected for sinus augmentation were grafted with biphasic calcium phosphate, whereas the other half were grafted with autogenous bone chips harvested intraorally. After 9 months of healing, bone cores were retrieved from implant sites for histologic and histomorphometric evaluation.Results: the areas augmented with autogenous bone chips showed newly formed bone with a pattern very similar to that of the native area. Histomorphometry demonstrated that the amount of newly formed bone in the autogenous bone group was significantly greater than in the biphasic calcium phosphate group (P < 0.05). in the biphasic calcium phosphate group, less bone formation was observed in the area further away from native bone interface than in the area closer to native bone interface (P < 0.05), whereas no significant differences were observed between both areas in the autogenous group. in both groups, the implant survival rate was 100% with a minimum 1-year follow-up.Conclusion: the data presented in this work confirm the osteoconductive properties of biphasic calcium phosphate, as well as its use in association with maxillary sinus floor augmentation procedures with successful outcomes.
- ItemAcesso aberto (Open Access)Qualidade seminal, morfologia dos testículos e epidídimos de touros submetidos à dieta contendo gossipol(Universidade Federal de Minas Gerais, Escola de Veterinária, 2013-08-01) Santos, M.d.; Rodrigues, R.s.; Freitas, S.h.; Costa, D.s.; Ruas, J.r.m.; Miranda, E.j.; Simões, Manuel de Jesus [UNIFESP]; Universidade de Cuiabá Faculdade de Medicina Veterinária; UFMS Faculdade de Medicina Veterinária e Zootecnia; Epamig; Universidade Federal do Mato Grosso; Universidade Federal de São Paulo (UNIFESP)The objective was to evaluate the effect of intake of free gossypol on sperm quality and morphology of the testicles and epididymis of Nelore bulls. Twelve bulls were fed diets containing 3.3g of free gossypol/bull/day (cottonseed) (Group 1, n = 6) and a diet free of gossypol (Group 2, n = 6), respectively. Semen samples were collected in the beginning and end of the experiment which lasted 73 days. In the end of the study the testes and epididymis of bulls were removed to study the effect of free gossypol on histological characteristics. The average consumption of 3.3g of free gossypol/bull/day (mean 7.1mg of free gossypol/kg/day) reduced motility and sperm concentration and increased the percentage of major and total sperm defects, as well as the animals showing testes with seminiferous tubules of smaller thickness, fewer layers of spermatogenic lineage cells, smaller epididymal epithelium thickness and smaller number of sperm within the epididymal ducts, compared to animals with a diet free of gossypol (Group 2). The consumption of 3.3g of free gossypol/bull/day led to changes in morphology and morphometry of the testes and epididymis and reduced sperm quality of bulls.