Navegando por Palavras-chave "hemolysis"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    The Association of Serum 25-Hydroxyvitamin D With Biomarkers of Hemolysis in Pediatric Patients With Sickle Cell Disease
    (Lippincott Williams & Wilkins, 2018) Adegoke, Samuel Ademola [UNIFESP]; Braga, Josefina Aparecida Pellegrini [UNIFESP]; Adekile, Adekunle D.; Figueiredo, Maria Stella [UNIFESP]
    Although vitamin D deficiency (VDD) has been linked to anemia among sickle cell disease (SCD), its relationship with hemolysis is unclear. Serum 25-hydroxyvitamin D and biomarkers of hemolysis (hemoglobin [Hb]/hematocrit, reticulocyte percentage, absolute reticulocyte, and lactate dehydrogenase [LDH] levels) in 36 hydroxyurea-naive SCD children were quantified. Correlations were significantly positive with Hb/hematocrit (r=0.40, P=0.017
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Conformational changes of Loxosceles venom sphingomyelinases monitored by circular dichroism
    (Elsevier B.V., 2005-02-04) Andrade, S. A. de; Pedrosa, MFF; Andrade, RMG de; Oliva, MLV; van den Berge, C. W.; Tambourgi, D. V.; Inst Butantan; Universidade Federal de São Paulo (UNIFESP); Cardiff Univ
    Envenomation by arachnids of the genus Loxosceles can induce a variety of biological effects, including dermonecrosis and hemolysis. We have previously identified in L. intermedia venom two highly homologous proteins with sphingomyelinase activity, termed P1 and P2, responsible for all these pathological events, and also an inactive isoform P3. the toxins P1 and P2 displayed 85% identity with each other at the amino acid level and showed a 57% identity with SMase I, an active toxin from L. laeta venom. Circular dichroism was used to determine and compare the solution structure of the active and inactive isoforms. Effects of pH and temperature change on the CD spectra of the toxins were investigated and correlated with the biological activities. This study sheds new light on the structure-function relationship of homologous proteins with distinct biological properties and represents the first report on the structure-function relationship of Loxosceles sphingomyelinases D. (C) 2004 Elsevier Inc. All rights reserved.