Repositório Institucional - Unifesp :: Navegando por Palavras-chave "fibrosis"

Navegando por Palavras-chave "fibrosis"

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Amitriptyline aggravates the fibrosis process in a rat model of infravesical obstruction
(Wiley-Blackwell, 2012-06-01) Almeida Prado, Patricia S. de [UNIFESP]; Soares, Maria Fernanda Sanches [UNIFESP]; Lima, Flavio O.; Schor, Nestor [UNIFESP]; Teixeira, Vicente P. C. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal da Bahia (UFBA); Universidade de São Paulo (USP)
Infravesical obstruction (IVO) secondary to benign prostatic hypertrophy can affect up to 50% of men over 50 years old and may cause serious and irreversible alterations throughout the urinary tract, especially in the bladder. Therapeutic approaches are currently limited. Amitriptyline has recently been described as an analgesic, anti-inflammatory and myorelaxant in some experimental models. the objective of this study was to investigate the effects of amitriptyline hydrochloride on the process of fibrosis in a bladder outlet obstruction model in rats. Male Wistar rats were subjected to IVO and studied at intervals of 1 and 14 days postprocedure. the rats were randomly divided into five groups: sham, IVO1-T, IVO1-NT, IVO14-T and IVO14-NT. Bladder tissue was processed for histopathology, immunohistochemistry and RT-PCR. the IVO14 groups presented bladder fibrosis, smooth muscle cell hypertrophy and bladder wall thickening. the IVO14-T group demonstrated a higher intensity of fibrosis, higher macrophage infiltration rate and higher gene expression of Transforming growth factor (TGF) Tgf-beta 1. Thus this data shows that in this experimental mode amitriptyline had an amplifying effect on the process of fibrosis as a whole.
Acesso aberto (Open Access)
Editorial: Macrophages role in integrating tissue Signals and Biological Processes in Chronic inflammation and Fibrosis
(Frontiers Media Sa, 2017) Braga, Tarcio Teodoro; Moura, Ivan C.; Lepique, Ana Paula; Saraiva Camara, Niels Olsen [UNIFESP]
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Effect of ultrasound therapy on the repair of Gastrocnemius muscle injury in rats
(Elsevier B.V., 2008-09-01) Balejo Piedade, Maria Cristina [UNIFESP]; Galhardo, Milene Sanches [UNIFESP]; Battlehner, Claudia Naves; Ferreira, Marcelo Alves; Caldini, Elia Garcia; Szymanski de Toledo, Olga Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
The aim of this study was to evaluate the effect of the pulsed ultrasound therapy (PUT) in stimulating myoregeneration and collagen deposition in an experimental model of lacerative gastrocnemius muscle lesion in 30 Wistar rats. Fifteen rats were treated (TG) daily with 1 MHz pulsed ultrasound (50%) at 0.57 W/cm(2) for 5 min, and 15 were control animals (CG). Muscle samples were analyzed on postoperative days 4, 7 and 14 through H&E, Picrosirius-polarization and immunohistochemistry for desmin. the lesions presented similar inflammatory responses in both treated and control groups. the areal fraction of fibrillar collagen was larger in the TG at 4 days post-operatively (17.53 +/- 6.2% vs 6.79 +/- 1.3%, p = 0.0491), 7 days (31.07 +/- 7.45% vs 12.57 +/- 3.6%, p = 0.0021) and 14 days (30.39 +/- 7.3% vs 19.13 +/- 3.51%, p = 0.0118); the areal fraction of myoblasts and myotubes was larger in the TG at 14 days after surgery (41.66 +/- 2.97% vs 34.83 +/- 3.08%, p = 0.025). Our data suggest that the PUT increases the differentiation of muscular lineage cells, what would favor tissue regeneration. On the other hand, it is also suggested that there is a larger deposition of collagenous fibers, what could mean worse functional performance. However, the percentage of fibers seems to have stabilized at day 7 in TG and kept increasing in CG. Furthermore, the collagen supramolecular organization achieved by the TG is also significant according to the Sirius red staining results. (C) 2008 Elsevier B.V. All rights reserved.
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Estudo da fibrose no músculo esquelético mediada por receptores de angiotensina e tratamento do músculo lesionado por prp e terapia gênica
(Universidade Federal de São Paulo (UNIFESP), 2015-12-17) Stilhano, Roberta Sessa [UNIFESP]; Han, Sang Won Han [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
process, which frequently occurs in cases of deeper muscle injury, leads to imperfect tissue regeneration. The current therapies have a low efficacy for prevention or treatment of the complications caused by fibrosis, such as atrophy, contraction and pain followed by functional loss and post-traumatic fibrosis. Hypertension is a contributing factor in fibrosis, and angiotensin II (Ang II), which is the main responsible for vasoconstriction, seems to trigger signaling fibrosis. There is communication between TGFB1 and Ang II signaling pathway in cardiac muscle. In parallel, there is a discordant discussion of GM-CSF role in skeletal muscle fibrosis. To better understand the fibrotic process occurring after injury in skeletal muscle, first of all, we established a muscle injury model followed by suture, which produces a deep lesion developing fibrosis largely muscle. To evaluate the possible role of the AT1 and GM-CSF in promoting fibrosis, multiple vectors were constructed, including, Lv-mirAT1a expressing a microRNA to silence the expression of AT1a protein and Lv-GM-CSF expressing GM-CSF. Considering that the injection of lentiviral solution in skeletal muscle may not be efficient for transduction of these vectors and cause leakage to other tissues, an alginate hydrogel was formulated for carrying lentivetores. The animals treated with alginate hydrogel loaded with Lv-GM-CSF and Lv-mirAT1a drastically reduced muscle fibrosis. Thus, it was inferred that the AT1a should participate in the fibrotic process of skeletal muscle and its inhibition is a good strategy for reducing post-injury fibrosis. On the other hand, the effect of the expression of GM-CSF in the control of fibrosis varied depending on the inflammatory progression, or its expression and the onset of inflammation-accentuated fibrosis, but expression in late stage drastically reduced fibrosis, confirming the results of our previous work. The mediators of fibrosis, such as angiotensin and growth factors are present in plasma and in sports medicine, the platelet rich plasma (PRP) is widely used to treat various types of lesions, including muscle injury. In an attempt to correlate the above studies with these factors in PRP, the molecular and cellular content of that preparation was evaluated. Two methods for preparation of PRP were established in the literature database and were named LPRP and PPRP. The main difference between the two preparations is the presence of leukocytes (LPRP) or not (PPRP), but the molecular and cellular contents thereof vary markedly PRPs. NIH3T3 cell lines (fibroblasts) and C2C12 (myoblast) grown with 1% PRP, and 10% FBS promoted cell proliferation in different patterns. The expression gene expression profile of 6 growth factors were analyzed and showed that each PRP for each cell type expression level of these genes is significantly variable. In conclusion, it was demonstrated that AT1 participates in skeletal muscle fibrosis and the inhibition of AT1 via microRNA is a good alternative to reduce fibrosis. Forced expression of GM-CSF early in the muscle injury promotes the formation of fibrosis, but their expression days later drastically reduces fibrosis. For late expression of GM-CSF via lentivetor contained in loco, the alginate hydrogel formulation with lentivetor developed here was ideal. As for PRPs, leukocytes contained in LPRP takes the PRP formation with different quality and amount of cells and molecules, hence its effect on the target cells were different.
Acesso aberto (Open Access)
Estudo de membrana biológica em ratos na prevenção de fibrose pós laminectomia
(Academia Brasileira de Neurologia - ABNEURO, 2006-06-01) Herculano, Marco Antonio; Tella Jr, Oswaldo Ignácio De [UNIFESP]; Prandini, Mirto Nelso [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Faculdade de Medicina de Jundiaí; Universidade Federal de São Paulo (UNIFESP)
The prevention of fibrosis after lumbar and thoracic laminectomies by avoiding herniation of muscular tissue was studied using Wistar-EPM rats with a biological membrane made of decorticated bone of bovine material. The rats were sacrified after eight, sixteen and twenty four weeks and the materal was sent to anatomopathological study. This membrane proved to be biocompatible and its efficacy was seen by allowing formation of bone and preventing muscular tissue invasion of the epidural space and avoiding adherences.
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Expressão de fatores indutores de fibrose no tecido cavernoso de pacientes com disfunção erétil pós prostatectomia radical
(Universidade Federal de São Paulo (UNIFESP), 2015-12-15) Cabrini, Marcelo Rodrigues [UNIFESP]; Ribeiro, Cassio Andreoni Ribeiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Objectives: To characterize transforming growth factor beta 1 (TGF?1) and related signaling pathway proteins in a large cohort of human penile tissue (HPT) samples from men with post-radical prostatectomy (RP) erectile dysfunction (ED). Method: HPT was collected from patients undergoing penile prosthesis implantation for postradical prostatectomy erectile dysfunction (RP-ED; n = 57). Two groups of tissue were used for comparison: organic ED (O-ED; n = 30), also collected during penile prosthesis implantation, and control (CON; n = 6), collected from patients undergoing partial penectomy, without ED. Western blot analysis was performed to investigate the protein expressions of TGF?1, thrombospondin 1 (TSP1; an activator of TGF?1), a family of transcriptional factors activated by TGF?1 (Smad2, phospho-Smad2- serine-465/467 [pSmad2], Smad3, phospho-Smad3-serine-423/425 [pSmad3]), and fibronectin (FN; an extracellular matrix glycoprotein induced by TGF?1). Results: Expressions of TGF?1 and TSP1 were significantly higher in RP-ED (p <0.05) and OED (p <0.05) groups compared with that of the CON group and were not different between either ED groups. Expressions of Smad2, pSmad2, Smad3, pSmad3, and FN were similar among all groups. Within the RP-ED group, a subgroup analysis showed that body mass index (BMI)>25Kg/m2 was related to increased expression of TGF?1 and TSP1, previous history of intracavernosal injection was related to increased expression of TGF?1 (p<0.05), and time from RP to penile prosthesis implantation (moment of tissue collection) was related to increased expression of pSmad2 (p<0.05). Conclusions: Our results demonstrate that TSP1- and TGF?1- dependent fibrotic changes occur in penile tissue in patients with ED regardless of etiology. The unchanged expression of the Smad transcriptional factors may be reconciled by a Smad-independent downstream signaling pathway transmitting TGF?1 signals. Among RP-ED men, BMI, previous use of intracavernosal injection and time sice RP affect fibrotic protein expression.
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Factors associated with the intensity of liver fibrosis in renal transplant patients with hepatitis B virus infection
(Lippincott Williams & Wilkins, 2007-08-01) Matos, Carla Adriana Loureiro de [UNIFESP]; Perez, Renata de Mello [UNIFESP]; Lemos, Lara Barros [UNIFESP]; Pestana, Jose Osmar Medina [UNIFESP]; Lanzoni, Valeria Pereira [UNIFESP]; Albertoe, Fernando L.; Moreira, Eloisa S.; Silva, Antonio Eduardo Benedito [UNIFESP]; Ferraz, Maria Lucia Cardoso Gomes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Fleury Med Diagnost Ctr
Background Hepatitis B may show a more aggressive course after kidney transplantation, but the factors associated with the progression of fibrosis in this group have not been identified.Objectives To determine the influence of hepatitis B virus (HBV) viral load and host-related factors on the progression of hepatic fibrosis in hepatitis B virus-infected renal transplant recipients.Patients and methods Renal transplant patients positive for HBV surface antigen (HBsAg) and submitted to a liver biopsy because of evidence of viral replication were included. Patients with advanced fibrosis (METAVIR F3-F4) were compared with patients with mild fibrosis (F0-F2) regarding sex, age, estimated time since infection, post-transplant time, donor type, history of renal transplantation, alanine aminotransferase, anti-hepatitis C virus, HBeAg and quantitative hepatitis B virus-DNA. Logistic regression analysis was applied to identify variables independently associated with more advanced fibrosis.Results Fifty-five patients (75% men, 41 +/- 11 years) with a mean post-transplant time of 5 +/- 4 years were included. HBeAg was detected in 67% of the patients and anti-hepatitis C virus in 35%. The median hepatitis B virus-DNA level was 2.8 x 10(8) copies/ml. Seventeen (31%) patients had advanced fibrosis. Using logistic regression analysis, the only variable that showed an independent association with more advanced stages of fibrosis was post-transplant time (P=0.03, odds ratio: 1.2, 95% confidence interval: 1.02-1.45).Conclusion Hepatitis B virus viral load, although very high, and hepatitis B virus/hepatitis C virus coinfection are not related to the intensity of liver fibrosis in renal transplant patients infected with hepatitis B virus. Post-transplant time was the only factor independently associated with more advanced liver fibrosis, suggesting the influence of immunosuppression on the progression of liver disease in these patients.
Acesso aberto (Open Access)
Galectin-3: A Friend but Not a Foe during Trypanosoma cruzi Experimental Infection
(Frontiers Media Sa, 2017) da Silva, Aline A.; Teixeira, Thaise L.; Teixeira, Samuel C.; Machado, Fabricio C. [UNIFESP]; dos Santos, Marlus A.; Tomiosso, Tatiana C.; Tavares, Paula C. B.; e Silva Brigido, Rebecca T.; Martins, Flavia Alves; de Lira Silva, Nadjania S. [UNIFESP]; Rodrigues, Cassiano C.; Roque-Barreira, Maria C.; Mortara, Renato A. [UNIFESP]; Lopes, Daiana S.; Rodrigues Avila, Veridiana de Melo; da Silva, Claudio V.
Trypanosoma cruzi interacts with host cells, including cardiomyocytes, and induces the production of cytokines, chemokines, metalloproteinases, and glycan-binding proteins. Among the glycan-binding proteins is Galectin-3 (Gal-3), which is upregulated after T. cruzi infection. Gal-3 is a member of the lectin family with affinity for beta-galactose containing molecules; it can be found in both the nucleus and the cytoplasm and can be either membrane-associated or secreted. This lectin is involved in several immunoregulatory and parasite infection process. Here, we explored the consequences of Gal-3 deficiency during acute and chronic T. cruzi experimental infection. Our results demonstrated that lack of Gal-3 enhanced in vitro replication of intracellular parasites, increased in vivo systemic parasitaemia, and reduced leukocyte recruitment. Moreover, we observed decreased secretion of pro-inflammatory cytokines in spleen and heart of infected Gal-3 knockout mice. Lack of Gal-3 also led to elevated mast cell recruitment and fibrosis of heart tissue. In conclusion, galectin-3 expression plays a pivotal role in controlling T. cruzi infection, preventing heart damage and fibrosis.
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Hepatitis C in chronic kidney disease: Predialysis patients present more severe histological liver injury than hemodialysis patients?
(Karger, 2007-01-01) Lemos, Lara B.; Perez, Renata M.; Lemos, Marcelo M.; Lanzoni, Valeria P.; Draibe, Sergio A.; Souza e Silva, Ivonete Sandra de [UNIFESP]; Silva, Antonio Eduardo B.; Ferraz, Maria Lucia G.; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Rio de Janeiro (UFRJ)
Background: the characteristics of hepatitis C virus (HCV) infection in predialysis patients are poorly understood and they could be different from hemodialysis patients. Aims: To evaluate the demographics, laboratory and histological characteristics of chronic HCV infection in predialysis patients and to compare them with those observed in hemodialysis patients. Methods: Thirty-nine predialysis patients with chronic HCV infection were compared to HCV-infected hemodialysis patients (ratio of 1: 3) in terms of demographics, laboratory and histological characteristics. the fibrosis progression rate (FPR) was calculated as the ratio between fibrosis stage and duration of infection. Results: Predialysis patients were older (57 8 10 vs. 45 8 12 years; p < 0.001), presented a higher proportion of elevated alanine aminotransferase (71.8 vs. 41.0%; p = 0.001) and aspartate aminotransferase (64.1 vs. 26.5%; p < 0.001), a higher proportion of interface hepatitis (66.7 vs. 47%; p = 0.033) and more advanced fibrosis (71.8 vs. 16.2%; p = 0.001). Among patients with estimated duration of infection, predialysis patients presented a longer duration of infection (22 vs. 6 years; p < 0.001) and no difference in FPR was observed between groups (p = 0.692). Conclusion: Although predialysis patients with HCV infection present more severe histological injury than hemodialysis patients, this finding probably reflects a longer duration of infection with no evidence supporting that hepatitis C presents a more aggressive course in this group. Copyright (c) 2007 S. Karger AG, Basel
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Inhibition of peridural fibrosis after laminectomy using biological sheet in rat model (abstract).
(Assoc Arquivos De Neuro- Psiquiatria, 2004-12-01) Herculano, Marco Antonio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
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Insulin resistance and not steatosis is associated with modifications in oxidative stress markers in chronic hepatitis C, non-3 genotype
(Taylor & Francis Ltd, 2009-01-01) Oliveira, Ana C. [UNIFESP]; Parise, Edison Roberto [UNIFESP]; Catarino, Regina M. [UNIFESP]; Lanzoni, Valeria Pereira [UNIFESP]; Leite-Mór, Marilisa Moraes Barros [UNIFESP]; Simon, Karin Argenti [UNIFESP]; Junqueira, Virginia B. C. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Background: Modifications of oxidative stress are reported in hepatitis C. the relationship between insulin resistance (IR), steatosis and oxidative stress is not established. Materials and methods: One hundred and eighty-seven HCV-RNA patients were assessed by determination of biochemical, metabolic and viral features, HOMA-IR and morphological alterations. in the 52-non-3 genotypes sub-group and 35 healthy individuals, thiobarbituric acid (TBARS), total glutathione (total-GSH), vitamins C and E, lycopene, beta-carotene, glutathione peroxidase (GPx), catalase and superoxide dismutase were determined. Results: in non-3 genotype patients, steatosis was associated with higher values of BMI, HOMA-IR and triglycerides. in the 52-HCV sub-group, values of TBARS, GPx and total-GSH differ from the control group. Despite these, differences could not be observed according to the presence of steatosis, patients with IR presented significant differences regarding total-GSH (p = 0.019), beta-carotene (p = 0.006), lycopene (p = 0.005) and GPx (p = 0.009). Conclusion: in non-3 genotype HCV carries, IR, and not steatosis, is associated with modifications in serum levels of oxidative stress.
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Interactions between p-Akt and Smad3 in injured muscles initiate myogenesis or fibrogenesis
(Amer Physiological Soc, 2013-08-01) Dong, Yanjun; Lakhia, Ronak; Thomas, Sandhya S.; Dong, Yanlan; Wang, Xiaonan H.; Santos Silva, Kleiton Augusto [UNIFESP]; Zhang, Liping; Baylor Coll Med; Emory Univ; Capital Med Univ; Universidade Federal de São Paulo (UNIFESP)
In catabolic conditions such as aging and diabetes, IGF signaling is impaired and fibrosis develops in skeletal muscles. To examine whether impaired IGF signaling initiates muscle fibrosis, we generated IGF-IR+/- heterozygous mice by crossing loxP-floxed IGF-IR (exon 3) mice with MyoD-cre mice. IGF-IR+/- mice were studied because we were unable to obtain homozygous IGF-IR-KO mice. in IGF-IR+/- mice, both growth and expression of myogenic genes (MyoD and myogenin; markers of satellite cell proliferation and differentiation, respectively) were depressed. Likewise, in injured muscles of IGF-IR+/- mice, there was impaired regeneration, depressed expression of MyoD and myogenin, and increased expression of TGF-beta 1, alpha-SMA, collagen I, and fibrosis. To uncover mechanisms stimulating fibrosis, we isolated satellite cells from muscles of IGF-IR+/- mice and found reduced proliferation and differentiation plus increased TGF-beta 1 production. in C2C12 myoblasts (a model of satellite cells), IGF-I treatment inhibited TGF-beta 1-stimulated Smad3 phosphorylation, its nuclear translocation, and expression of fibronectin. Using immunoprecipitation assay, we found an interaction between p-Akt or Akt with Smad3 in wild-type mouse muscles and in C2C12 myoblasts; importantly, IGF-I increased p-Akt and Smad3 interaction, whereas TGF-beta 1 decreased it. Therefore, in muscles of IGF-IR+/- mice, the reduction in IGF-IR reduces p-Akt, allowing for dissociation and nuclear translocation of Smad3 to enhance the TGF-beta 1 signaling pathway, leading to fibrosis. Thus, strategies to improve IGF signaling could prevent fibrosis in catabolic conditions with impaired IGF signaling.
Acesso aberto (Open Access)
Metformin Mitigates Fibrosis and Glucose Intolerance Induced by Doxorubicin in Subcutaneous Adipose Tissue
(Frontiers Media Sa, 2018) Biondo, Luana A.; Batatinha, Helena A.; Souza, Camila O.; Teixeira, Alexandre A. S.; Silveira, Loreana S.; Alonso-Vale, Maria Isabel C. [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Alves, Michele J.; Seelaender, Marilia [UNIFESP]; Neto, Jose C. R.
Doxorubicin (DX) is a chemotherapeutic drug that is used in clinical practice that promotes deleterious side effects in non-tumor tissues such as adipose tissue. We showed that DX leads to extensive damage in adipose tissue via a disruption in 5'-adenosine monophosphate-activated protein kinase (AMPK) and PPAR-gamma signaling. Thus, we investigated whether co-treatment with the biguanide drug metformin (MET) could prevent the side effects of DX through the activation of AMPK in adipose tissue. The goal of the present study was to verify the effects of DX and adjuvant MET treatment in subcutaneous adipose tissue (SAT) and to determine whether MET could protect against chemotherapy-induced side effects. C57/BL6 mice received DX hydrochloride (2.5 mg/kg) intraperitoneally 2 times per week for 2 weeks (DX), concomitantly or not, with MET administration (300 mg/kg oral daily) (DX + MET). The control group (CTRL) was pair-fed according to the food consumption of the DX group. After euthanasia, adipose tissue fat pads were collected, and SAT was extracted so that adipocytes could be isolated. Glucose uptake was then measured, and histological, gene, and protein analyses were performed. One-way analysis of variance was also performed, and significance was set to 5%. DX reduced retroperitoneal fat mass and epididymal pads and decreased glycemia. In cultured primary subcutaneous adipocytes, mice in the DX group had lower glucose uptake when stimulated with insulin compared with mice in the CTRL group. Adipocytes in the DX group exhibited a reduced area, perimeter, and diameter
Acesso aberto (Open Access)
Survival in idiopathic pulmonary fibrosis - cytotoxic agents compared to corticosteroids
(W B Saunders Co Ltd, 2006-02-01) Pereira, Carlos Alberto de Castro [UNIFESP]; Nunes, Tatiana Santos Malheiros [UNIFESP]; Coletta, Ester Nei Aparecida Martins [UNIFESP]; Ferreira, Rimarcs Gomes [UNIFESP]; Rubin, Adalberto Sperb; Ota-Arakaki, Jaquelina Sonoe [UNIFESP]; Rocha, Nailê Sena da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Study objective: To compare the survival of patients with IPF treated retrospectively with corticosteroids atone, to survival of patients treated with immunosuppressive and corticosteroids combined. Design: Non-randomized retrospective cohort study.Setting: Three tertiary centers in Brazil.Patients: Eighty-two IPF patients were included. the diagnosis was confirmed by open lung biopsy in 48. Patients received either corticosteroids alone (group 1) or cytotoxic agents in addition to corticosteroids (group 11).Measurements and results: the primary end-point was mortality. Secondary outcome included longitudinal changes in FVC. Mean age was 66+8 years. FVC was 71 +/- 17% of predicted. There were 48 deaths during the study period (59%), 44 secondary to respiratory causes. From preliminary univariate analysis, for the group as a whole, worse survival was found to be associated with FVC% < 70% of predicted (P = 0.004), evidence of disease progression by follow-up FVC measurements (P = 0.01), and pharmacologic treatment (P = 0.014). Median survival was 25 months for the group 1, and 45 months for the group 11 (Log-Rank = 6.45, P = 0.01). After adjusting for FVC >= 70% and < 70% of predicted, there was evidence to indicate that survival was associated with recommended pharmacologic treatment only in patients with FVC >= 70% (Log Rank = 6.84, P = 0.009).Conclusions: the combination of immunosuppressive agents and prednisone results in better survival when compared to prednisone alone in patients with IPF the benefit seems to occur only in patients with less severe disease, as reflected by FVC >= 70%. (c) 2005 Elsevier B.V. All rights reserved.
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Uso de membrana amniótica para reduzir a formação de aderências em cirurgia de estrabismo: estudo experimental em coelhos
(Universidade Federal de São Paulo (UNIFESP), 2015-08-31) Kirsch, David [UNIFESP]; Sato, Elcio Hideo Sato [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Purpose: 1) To evaluate the anti-inflammatory effect of amniotic membranes in strabismus surgery. 2) To evaluate the effect of amniotic membranes in reducing fibrosis in strabismus surgery. 3) To evaluate the effect of amniotic membranes in reducing adhesion formation and ocular motility restrictions following strabismus surgery. Methods: The study was divided into 2 stages. In the first stage, 20 rabbits underwent superior rectus muscle recession surgery in both eyes. Surgery was performed in the same manner in both eyes, but in the right eye, after the recession, a cryopreserved human amniotic membrane was placed over the muscle without sutures, with the epithelial side facing down and in contact with the sclera. After 15 days, the rabbits were sacrificed, and their orbits were exenterated and evaluated histopathologically to quantify tissue inflammation and fibrosis. In the second stage, 7 rabbits underwent the same procedure but were sacrificed at 30 days. Prior to sacrificing the rabbits, a dynamometer was used to measure the force required to displace all the eyes. Results: In the first group of rabbits, whose eyes were exenterated at 15 days, the eyes with amniotic membranes exhibited an increased inflammatory response and less fibrosis than the control eyes without amniotic membranes. At 30 days, the eyes with amniotic membrane continued to exhibit increased inflammation and less fibrosis than the eyes without amniotic membranes. In the dynamometer test, more force was needed to displace the eye without amniotic membrane after 15 days, but there was no significant difference between the forces required at 30 days. Conclusions: Cryopreserved human amniotic membranes used to treat rabbits led to an increase in the inflammatory process following strabismus surgery but decreased fibrosis formation. At 30 days, there was no notable difference.

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