Navegando por Palavras-chave "fetus"

Agora exibindo 1 - 14 de 14
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Avaliação do índice de performance miocárdica em fetos com restrição do crescimento
    (Universidade Federal de São Paulo (UNIFESP), 2015-12-31) Silva, Carolina Pacheco [UNIFESP]; Nardozza, Luciano Marcondes Machado Nardozza [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: This study aims to evaluate the Myocardial Performance Index in fetuses with growth restriction (IUGR) compared with healthy fetuses. To assess the intra and interobserver repeatability of the method. Method: We conducted a prospective cross-sectional study in pregnant women between 24 and 34 weeks, with singleton pregnancies, divided in three groups (controls, restricted fetuses below the 3rd percentile and restricted between percentiles 3 and10). For the estimation of fetal weight (EPF), we used the formula of Hadlock 4. The fetal modified Myocardial Performance Index was assessed in all fetuses. Assessed restricted fetuses umbilical artery Doppler normal. Regarding the repeatability of the method used the intraclass correlation coefficient (ICC) and Bland-Altman. Results: There was no statistically significant difference between the values found in the IPM-mod among fetuses with growth restriction below the 3rd percentile (0.36 SD ± 0.06), fetuses between percentiles 3:10 (0.35 ± 0.05) and normal fetuses (0.32 ± 0.05). Regarding the repeatability, the intraclass correlation coefficient (ICC) was 0.726 intra and interobserver 0.76. Conclusion: The mod-IPM is an important tool for assessment of fetal cardiac function and the values found for fetuses with FGR with normal umbilical artery Doppler and healthy fetuses were not statistically significant, suggesting that at this stage of RCF these fetuses still present normal cardiac function. This valuation method proved reproducible, but requires a substantial number of measurements for non-experienced examiner to achieve competence
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Cell proliferation and death in the gastric epithelium of developing rats after glucocorticoid treatments
    (Wiley-Blackwell, 2000-11-01) Gama, Patricia; Goldfeder, Eliane Maria [UNIFESP]; De Moraes, Julio Cesar Bertoccini; Alvares, Eliana Parisi; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Glucocorticoids take part in the intense morphofunctional modifications that occur in the gastric mucosa during fetal and postnatal development. Two studies were designed to evaluate corticoids role in gastric cell proliferation and apoptosis in developing rats: in vivo, using suckling animals; in vitro, using gastric explants obtained from 20-day fetuses. These explants were cultured in DMEM/F12 medium treated or not with 50 ng/ml of corticosterone; after 22 hr, vincristine was added to the medium for 2 hr to block mitosis. The metaphasic index decreased significantly after the 24-hr treatment (controls: 1.52 +/- 0.53; treated: 0.40 +/- 0.21) and apoptotic cells were visualized under light and electron microscopy. Fifteen-day-old rats were treated with hydrocortisone (25 mg/Kg) for 3 days, and injected with BrDU (100 mg/Kg) 1 hr before sacrifice on the 18(th) day. BrDu-labeled and non-labeled cells were counted to determine the labeling index of epithelial cells. As apoptotic cells are rapidly eliminated, other animals were treated for only 2-3 hr. Sections were investigated for the presence of apoptotic cells, using morphological criteria and TUNEL labeling. Hydrocortisone significantly reduced the labeling index (controls: 15.6 +/- 1.6 vs. treated: 11.7 +/- 1.1), besides altering the body weight gain. Hydrocortisone treatment doubled the number of apoptotic cells after 2 hr, and quadruplicated it after 3 hr. The results demonstrated that glucocorticoids inhibit cell proliferation in the gastric epithelium of fetuses and suckling rats and increase apoptotic rates, suggesting the exit from cell cycle. Anat Rec 260: 213-221, 2000. (C) 2000 Wiley-Liss, Inc.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Comparison of the two-dimensional and multiplanar methods and establishment of a new constant for the measurement of fetal lung volume
    (Taylor & Francis Ltd, 2008-01-01) Junior, Edward Araujo [UNIFESP]; Nardozza, Luciano Marcondes Machado [UNIFESP]; Pires, Claudio Rodrigues [UNIFESP]; Filho, Helio Antonio Guimaes [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objectives. To compare the two-dimensional (2D) and multiplanar methods in the measurement of lung volume in normal fetuses, to obtain a new constant to be incorporated into the 2D equation, and to apply the new equation in fetuses with pulmonary hypoplasia (PH) confirmed postnatally.Methods. A cross-sectional study was performed on 51 pregnant women at between 20 and 35 weeks of gestation. the ellipsoid formula (x x y x z x 0.52) was used to calculate lung volume by the 2D method. A sequence of multiple planes with 2.0-mm intervals was used with the multiplanar method. in order to compare the techniques, the intra-class correlation coefficient (ICC) and the Student's t-test (p) were used. First-order linear regressions were used to establish a new constant, with three-dimensional (3D) lung volume as dependent variable and gestational age and 2D volume as independent variables. in order to validate it, the new equation was applied to 11 fetuses with confirmed lethal PH.Results. the mean volumes obtained by the 2D method were overestimated when compared to the multiplanar method (right lung: 23.87 vs. 18.26mL, respectively, p < 0.001 and left lung: 16.18 vs. 14.33mL, respectively, p=0.008). Using a first-order polynomial regression, new constants were obtained for the right lung (0.152) and for the left lung (0.167). When compared to the traditional formula, the new equation presented higher sensitivity (18.1%) in predicting lethal PH.Conclusion. the recalculated 2D equation can be a promising alternative to 3D ultrasonography in the prediction of PH.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Comparison of two- and three-dimensional ultrasonography in lung volume measurement of normal fetuses
    (Walter de Gruyter Gmbh, 2007-01-01) Araujo, Edward; Machado Nardozza, Luciano Marcondes; Pires, Claudio Rodrigues; Guimaraes Filho, Helio Antonio; Moron, Antonio Fernandes; Universidade Federal de São Paulo (UNIFESP)
    Aim: the purpose of this study was to compare the two-and three-dimensional methods for measuring fetal lung volume of normal fetuses.Methods: A cross-sectional study was performed with 51 normal pregnant women between 20 and 35 weeks. the ellipsoid formula (X*Y*Z*0.52) was used for volume calculation with the two-dimensional (2D) method. With the VOCAL (TM) (Virtual Organ Computer-aided Analysis) method, a rotation angle of 30 degrees was used. Intraclass correlation coefficient (ICC), paired Student's t-test. and Bland-Altman plots were used to compare the techniques. To calculate the intraobserver variability we used the ICC and compared the means between the two measures using the paired Student's p-test.Results: VOCAL (TM) and 2D methods were highly correlated (ICC = 0.919, and 0.873 for the right and left lungs, respectively), however, there was a disagreement. the fetal lung volume means calculated by the 2D method were always overestimated in relation to the means obtained by the VOCAL (TM), for the right lung (24.02 mL x 19.15 mL; P < 0.001), as well as for the left (16.03 mL x 13.77 mL; P = 0.002). As for the intraobserver variability, a good reproducibility was observed for the volume measurement of the left lung by the 2D technique (mean=0.40 mL.; P=0.57) and by the VOCAL (TM) (mean= -0.22 mL; P=0.63). the 2D method, however, presented low reproducibility for the right lung (mean = 1.73 mL; P = 0.31).Conclusion: the two-dimensional method had low agreement and low reproducibility in relation to the threedimensional method for measurement of fetal lung volume in normal fetuses.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Cord blood cardiac troponin I, fetal Doppler velocimetry, and acid base status at birth
    (Elsevier B.V., 2008-02-01) Alexandre, Sandra Maria [UNIFESP]; D'Almeida, Vania [UNIFESP]; Guinsburg, Ruth [UNIFESP]; Nakamura, Mary U. [UNIFESP]; Tufik, Sergio [UNIFESP]; Moron, Antonio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To analyze the association between umbilical cord cardiac troponin I (cTnl), obstetric Doppler, and birth acidemia. Method: This prospective observational study was conducted on 58 singleton pregnancies at 3 Brazilian hospitals. Umbilical and middle cerebral artery Doppler velocimetry were performed 24 h prior to birth. At delivery, cord blood was collected for pH, blood gas analysis, and cTnl measurement. Results: Cardiac troponin I >= 0.20 ng/mL was detected in 5 neonates (8.6%). Centralization was recorded in 12 fetuses; 4 of these (33.3%) had detectable cTnl compared with 1/46 (2.2%) fetuses with normal Doppler (P=0.005). Acidemia was present in 60% of the neonates with detectable cTnl, compared with 15% of the neonates with undetectable cTnl (P=0.042). Conclusion: Centralization and birth acidemia are associated with detectable cTnl in cord blood supporting the possibility of myocardial ischemia in these fetuses. (c) 2007 Published by Elsevier Ireland Ltd. on behalf of International Federation of Gynecology and Obstetrics.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Effects of chronic stavudine exposure on liver, pancreas and kidneys of pregnant rats and their fetuses: Morphological and biochemical aspects
    (Elsevier B.V., 2006-09-01) Barreto, Regina Lucia Braga; Soares, Jose Maria; Simoes, Ricardo Santos; Maciel, Gustavo Arantes Rosa; Jesus Simoes, Manuel de; Kulay, Luiz; Universidade Federal de São Paulo (UNIFESP)
    Objective: To study the morphological and biochemical effects on liver, pancreas and kidney of pregnant rats and their fetuses subjected to stavudine treatment.Methods: Forty animals were distributed in four groups E1, E2, E3, and C (control) and received by gavage once a day 1, 3 or 9 mg/kg of stavudine in 2 mL distilled water, from days 1 to 20 of pregnancy. After this period, the animals were sacrificed; blood samples were collected for further determinations of AST, ALT, creatinine, urea, glucose and amylase. Samples of liver, kidneys and pancreas of every rat and of the corresponding fetuses were taken and examined under light microscopy.Results: the maternal livers of groups E1, E2 and E3 displayed progressive morphological alterations without corresponding changes in serum AST and ALT activity. Maternal kidney histology and function were similar in all groups. Maternal pancreas of groups E2 and E3 evidenced moderate and progressive signs of tissue damage without functional repercussion. All fetal livers, kidneys and pancreas presented normal morphology.Conclusions: High doses of stavudine produced signs of mild to moderate maternal hepatic and pancreatic toxicity at the morphological level. This was not followed by changes in biochemical parameters, most conceivably due to the functional reserve of these organs. (C) 2006 Elsevier Ireland Ltd. All rights reserved.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Fetal frontomaxillary facial angle between 11 and 13+6 weeks of gestation in a Brazilian population: influence of different races
    (Informa Healthcare, 2013-07-01) Nascimento Panigassi, Ana Paula [UNIFESP]; Araujo Junior, Edward [UNIFESP]; Machado Nardozza, Luciano Marcondes [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Silva Pares, David Baptista da [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate the influence of different races over the measurement of the frontomaxillary facial angle between 11 and 13 + 6 weeks of pregnancy in a Brazilian population.Methods: A cross-sectional study was conducted with 332 healthy pregnant women, with a crown-rump length (CRL) between 47 and 84 mm. Such measurements were taken abdominally, using the mid-sagittal plane, and the angle was measured by tracing a line over the palate and a line from the anterosuperior maxillary angle all the way to the external part of the forehead. As for the reference intervals, a simple linear regression between the frontomaxillary facial angle and the CRL was used, as well as Pearson's correlation coefficient (r). To evaluate the difference between races, a variance analysis was used (ANOVA). To calculate reproducibility, the intraclass correlation coefficient (ICC) was used.Results: the means for the fetal frontomaxillary facial angle in white, black and mixed races were 81.8 +/- 6.6; 82.2 +/- 6.1 and 81.4 +/- 6.2 mm, respectively. There was no statistical difference between races (p = 0.713). A decreasing correlation between the frontomaxillary facial angle and the CRL was observed for the black (r = -0.450) and mixed (r = -0.212) races. Excellent intraobserver reproducibility was observed, as well as a satisfactory interobserver reproducibility, with ICC of 0.858 and 0.605, respectively.Conclusion: There were no significative statistical differences in the measurement of the fetal frontomaxillary facial angle between 11 and 13 + 6 weeks of pregnancy in the different races in a Brazilian population.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Neurological Outcome in Fetuses with Mild and Moderate Ventriculomegaly
    (Federacao Brasileira Soc Ginecologia & Obstetricia-Febrasgo, 2016) Tonni, Gabriele; Vito, Ida; Palmisano, Marcella; Martins, Wellington de Paula; Araujo Junior, Edward [UNIFESP]
    Introduction Ventriculomegaly (VM) is one the most frequent anomalies detected on prenatal ultrasound. Magnetic resonance imaging (MRI) may enhance diagnostic accuracy and prediction of developmental outcome in newborns. Purpose The aim of this study was to assess the correlation between ultrasound and MRI in fetuses with isolated mild and moderate VM. The secondary aim was to report the neurodevelopmental outcome at 4 years of age. Methods Fetuses with a prenatal ultrasound (brain scan) diagnosis of VM were identified over a 4-year period. Ventriculomegaly was defined as an atrial width of 1015 mm that was further divided as mild (10.1-12.0 mm) and moderate (12.1-15.0 mm). Fetuses with VM underwent antenatal as well as postnatal follow-ups by brain scan and MRI. Neurodevelopmental outcome was performed using the Griffiths Mental Development Scales and conducted, where indicated, until 4 years into the postnatal period. Results Sixty-two fetuses were identified. Ventriculomegaly was bilateral in 58% of cases. A stable dilatation was seen in 45% of cases, progression was seen in 13%, and regression of VM was seen in 4.5% respectively. Fetal MRI was performed in 54 fetuses and was concordant with brain scan findings in 85% of cases. Abnormal neurodevelopmental outcomes were seen in 9.6% of cases. Conclusion Fetuses in whom a progression of VM is seen are at a higher risk of developing an abnormal neurodevelopmental outcome. Although brain scan and MRI are substantially in agreement in defining the grade of ventricular dilatation, a low correlation was seen in the evaluation of VM associated with central nervous system (CNS) or non-CNS abnormalities.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Reference range of fetal cisterna magna volume by three-dimensional ultrasonography using the VOCAL method
    (Informa Healthcare, 2014-07-01) Araujo Junior, Edward [UNIFESP]; Passos, Ana Paula [UNIFESP]; Bruns, Rafael Frederico; Machado Nardozza, Luciano Marcondes [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Paraná (UFPR)
    Objective: To determine reference values for the fetal cisterna magna volume by three-dimensional ultrasonography (3DUS) using the virtual organ computer-aided analysis (VOCAL) method.Methods: A cross-sectional study was conducted with 224 healthy pregnant women between 17 and 29 completed weeks. Measurement of the fetal cisterna magna volume was performed in the standard axial plane of the transverse diameter of the cerebellum through the VOCAL method with a 30 degrees angle. for the determination of reference values, polynomial regressions with adjustments by the coefficient of determination (R-2) were held. the reliability and agreement were made by the intra-class correlation coefficient (ICC) and limits of agreement of the Bland-Altman graph.Results: the average of the fetal cisterna magna volume ranged from 0.73 +/- 0.25 to 3.79 + 1.10 cm(3) between 17 and 29 weeks, respectively. Correlation was observed between the fetal cisterna magna volume and the gestational age (GA), best represented by a quadratic equation: -1.918+0.0284 x GA + 0.0065 x GA(2) (R-2 = 0.67). It was observed good reliability and intra-observer agreement, with ICC = 0.92 and 95% limits of agreement (-49.7; 48.4). There was low inter-observer reliability and agreement, with ICC = 0.58 and 95% limits of agreement (-114.0; 80.2).Conclusion: Reference values for the fetal cisterna magna volume by 3DUS using the VOCAL method were determined and showed good agreement and intra-observer reliability.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Reference Range of Fetal Transverse Cerebellar Diameter Between 18 and 24 Weeks of Pregnancy in a Brazilian Population
    (Sage Publications Inc, 2015-02-01) Araujo Junior, Edward [UNIFESP]; Martins, Wellington P.; Machado Nardozza, Luciano Marcondes [UNIFESP]; Pires, Claudio Rodrigues; Zanforlin Filho, Sebastiao Marques; Referral Ctr Teaching Diagnost Imaging; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Ultrasonog & Retraining Med Sch Ribeirao Preto
    To determine a reference range of fetal transverse cerebellar diameter in Brazilian population. This was a retrospective cross-sectional study with 3772 normal singleton pregnancies between 18 and 24 weeks of pregnancy. the transverse cerebellar diameter was measured on the axial plane of the fetal head at the level of the lateral ventricles, including the thalamus, cavum septum pellucidum, and third ventricle. To assess the correlation between transverse cerebellar diameter and gestational age, polynomial equations were calculated, with adjustments by the determination coefficient (R-2). the mean of fetal transverse cerebellar diameter ranged from 18.49 +/- 1.24 mm at 18 weeks to 25.86 +/- 1.66 mm at 24 weeks of pregnancy. We observed a good correlation between transverse cerebellar diameter and gestational age, which was best represented by a linear equation: transverse cerebellar diameter: -6.21 + 1.307*gestational age (R-2 = 0.707). We determined a reference range of fetal transverse cerebellar diameter for the second trimester of pregnancy in Brazilian population.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Reference Ranges of Fetal Cisterna Magna Length and Area Measurements by 3-Dimensional Ultrasonography Using the Multiplanar Mode
    (Sage Publications Inc, 2015-02-01) Passos, Ana Paula [UNIFESP]; Araujo Junior, Edward [UNIFESP]; Bruns, Rafael Frederico; Machado Nardozza, Luciano Marcondes [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Fed Parana
    This study aimed to establish reference values for the length and area of the fetal cisterna magna using the multiplanar mode of 3-dimensional ultrasonography. A cross-sectional study including 224 normal pregnant women between 17 weeks 0 days and 29 weeks 6 days of gestation was carried out. the area and length of the fetal cisterna magna were measured in the axial plane at the level of the cerebellar transverse diameter. Reliability was determined by intraclass correlation coefficient. the mean length and area of the fetal cisterna magna ranged from 0.50 +/- 0.10 to 0.79 +/- 0.18 cm and 0.95 +/- 0.18 to 3.09 +/- 0.62 cm(2), respectively. Intraobserver reliability for the length and area (intraclass coefficients: 0.86 and 0.91, respectively) and interobserver reliability (intraclass coefficients: 0.64 and 0.82, respectively) were good. Three-dimensional ultrasonography using the multiplanar mode is a reliable method for the determination of reference values for the length and area of the fetal cisterna magna.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Reference Values for the Length and Area of the Fetal Corpus Callosum on 3-Dimensional Sonography Using the Transfrontal View
    (Amer Inst Ultrasound Medicine, 2012-02-01) Araujo Junior, Edward [UNIFESP]; Visentainer, Milena [UNIFESP]; Simioni, Christiane [UNIFESP]; Ruano, Rodrigo; Nardozza, Luciano Marcondes Machado [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objectives-The purpose of this study was to determine reference values for the length and area of the fetal corpus callosum between 20 and 33 weeks' gestation using 3-dimensional sonography.Methods-A cross-sectional study was performed in 293 healthy pregnant women between 20 and 33 weeks' gestation. The length and area of the corpus callosum were obtained via the transfontal view with the metopic suture as an acoustic window using 3-dimensional sonographic aquisitions. Linear and weighted polynomial regression models were used, which were adjusted by residual analysis and the R-2 determination coefficient. Intraobserver and interobserver reproducibilities were analyzed by an intraclass correlation coefficient.Results-The mean corpus callosum length +/- SD varied from 19.52 +/- 2.24 to 40.36 +/- 2.87 mm, whereas the mean area varied from 0.44 +/- 0.11 to 1.47 +/- 0.21 cm(2) at 20 and 33 weeks, respectively. The length and area were highly correlated with gestational age: corpus callosum length = -52.41 + 4.71 x gestational age - 0.06 x gestational age(2) (R-2 = 0.868); and corpus callosum area = -2.47 + 0.16 x gestational age -0.000037 x gestational age(2) (R-2 = 0.765). The intraobserver and interobserver reproducibties were excellent, with intraclass correlation coefficients of 0.98 and 0.94 for the length and 0.99 and 0.90 for the area, respectively.Conclusions-Reference values for the length and the area of the fetal corpus callosum between 20 and 33 weeks' gestation were determined with high reproducibility.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Safety of ultrasonography in pregnancy: WHO systematic review of the literature and meta-analysis
    (Wiley-Blackwell, 2009-05-01) Torloni, Maria Regina [UNIFESP]; Vedmedovska, N.; Merialdi, M.; Betran, A. P.; Allen, T.; Gonzalez, R.; Platt, L. D.; ISUOG WHO Fetal Growth Study Grp; Brazilian Cochrane Ctr; Universidade Federal de São Paulo (UNIFESP); Riga Stradins Univ; WHO; Chilean Minist Hlth; Univ Calif Los Angeles
    Objective in the context of the planned International Society of Ultrasound in Obstetrics and Gynecology-World Health Organization multicenter study for the development of fetal growth standards for international application, we conducted a systematic review and meta-analysis to evaluate the safety of human exposure to ultrasonography in pregnancy.Methods A systematic search of electronic databases, reference lists and unpublished literature was conducted for trials and observational studies that assessed short- and long-term effects of exposure to ultrasonography, involving women and their fetuses exposed to ultrasonograpby, using B-mode or Doppler sonography during any period of pregnancy, for any number of times. the outcome measures were: (1) adverse maternal outcome; (2) adverse perinatal outcome; (3) abnormal childhood growth and neurological development, (4) non-right handedness; (5) childhood malignancy; and (6) intellectual performance and mental disease.Results the electronic search identified 6716 citations, and 19 were identified from secondary sources. A total of 6.1 publications reporting data from 41. different studies were included: 16 controlled trials,13 cohort and 12 case-control studies. Ultrasonography in pregnancy was not associated with adverse maternal or perinatal outcome, impaired physical or neurological development, increased risk for malignancy in childhood, subnormal intellectual performance or mental diseases. According to the available clinical trials, there was a weak association between exposure to ultrasonograpby and non-right handedness in boys (odds ratio 1.26; 95% CI, 1.03-1.54).Conclusion According to the available evidence, exposure to diagnostic ultrasonography during pregnancy appears to be safe. Copyright (C) World Health Organization (2009).
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Soluble Fas antigen in term labour
    (Elsevier B.V., 2006-10-01) Daher, S.; Moreira, J. R.; Dalboni, Maria Aparecida [UNIFESP]; Guimaraes, A. J.; Bevilacqua, E.; Mattar, Rosiane [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)