Navegando por Palavras-chave "embryo"
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- ItemSomente MetadadadosEmbryo vascularization by three-dimensional power Doppler ultrasonography at 7-10 weeks of pregnancy(Walter de Gruyter Gmbh, 2009-07-01) Bortoletti Filho, Joao [UNIFESP]; Marcondes, Luciano [UNIFESP]; Nardozza, Machado [UNIFESP]; Araujo Junior, Edward [UNIFESP]; Rolo, Liliam Cristine [UNIFESP]; Nowak, Paulo Martin [UNIFESP]; Guimaraes Filho, Helio Antonio [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Aim: Assess vascular indices of 7-10 week embryos using three-dimensional power Doppler (3DPD) and correlate them with the crown-rump length (CRL).Methods: This cross-sectional study included 65 healthy pregnancies between 7 and 10 weeks. the three-dimensional volume of the embryo was obtained using an endocavitary volumetric transducer and the VOCAL (Virtual Organ Computer-aided Analysis) method, with a 128 rotation angle and 15 sequential planes. the vascularization (VI), flow (FI) and the vascular and flow (VFI) indices were obtained using 3DPD and the mean, median, standard deviation, maximum and minimum values were calculated for each gestational age. Pearson's correlation coefficient (r) was used to evaluate the correlation between vascular indices and CRL.Results: the VI ranged from 0.77 to 41.67, mean 14.68 (+/- 8.60), the FI went from 25.71 to 139.50, mean 90.61 (+/- 21.51) and the VFI from 0.20 to 81.57, mean 15.69 (+/- 12.42). the correlation between CRL and all 3D power Doppler vascular indices was low (VI - r=-0.073, P=0.566; FI - r=0.173, P=0.168 and VFI - r=-0.004, P=0.974).Conclusion: 3D power Doppler vascular indices in 7 - 10 week embryos do not correlate with CRL.
- ItemSomente MetadadadosPHYSIOLOGY and ENDOCRINOLOGY SYMPOSIUM: Influence of cattle genotype (Bos indicus vs. Bos taurus) on oocyte and preimplantation embryo resistance to increased temperature(Amer Soc Animal Science, 2013-03-01) Paula-Lopes, F. F. [UNIFESP]; Lima, R. S.; Satrapa, R. A.; Barros, C. M.; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)High environmental temperatures during the hot months of the year reduce reproductive performance in cattle. Summer heat stress depression in fertility is a multifactorial problem; however, there is evidence that the bovine germinal vesicle and maturing oocyte, as well as the early embryo, are major targets of the deleterious effects of heat stress. Such adverse effects are less pronounced in heat-tolerant breeds (Bos indicus) than heat-sensitive breeds (Bos taurus). This genetic variation results from the greater thermoregulatory ability and cellular thermoresistance of heat-tolerant breeds. Heat-induced oocyte cellular damage occurs in both cytoplasmic and nuclear compartments. Heat shock has been shown to reduce oocyte nuclear maturation, induce apoptosis, compromise oocyte cytoskeleton, and impair oocyte mitochondrial function and developmental competence. However, the oocyte cytoplasm is more susceptible to heat shock than the nucleus. This effect is greater for Bos taurus than Bos indicus oocytes. the detrimental effects of heat shock are also critical during the first cleavage divisions when most of the embryonic genome is inactive; however, the bovine embryo becomes more resistant to increased temperature as it proceeds through development. Several studies demonstrated that Bos indicus embryos are more thermotolerant than Bos taurus embryos. Adaptive changes involved in acquisition of thermotolerance are likely derived from changes in gene expression and (or) activity of biochemical molecules that control cellular functions against stress. Recently, molecules such as IGF-I and caspase inhibitor z-DEVD-fmk have been shown to exert a thermoprotective role, rescuing heat-induced oocyte and embryo cellular damage and developmental competence. Therefore, cattle genotype and thermoprotective molecules can be considered as an alternative to modulate the effects of increased temperature in reproductive function. (C) 2013 American Society of Animal Science. All rights reserved.
- ItemSomente MetadadadosReference Range of Embryo Volume by 3-D Sonography Using the XI VOCAL Method at 7 to 10+6 Weeks of Pregnancy(Thieme Medical Publ Inc, 2010-06-01) Araujo Junior, Edward [UNIFESP]; Machado Nardozza, Luciano Marcondes [UNIFESP]; Rolo, Liliam Cristine [UNIFESP]; Nowak, Paulo Martin [UNIFESP]; Bortoletti Filho, Joao [UNIFESP]; Moron, Antonio Fernandes [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)We sought to establish normative data for three-dimensional ultrasound (3DUS) first-trimester embryonic volume using the extended imaging virtual organ computer-aided analysis (XI VOCAL) technique. This cross-sectional study involved 64 normal pregnancies at 7 to 10 + 6 weeks' gestation. Embryonic volume was obtained on 3DUS using the XI VOCAL technique with manual outline of 10 sequential planes. the mean, median, standard deviation, and maximum and minimum values were obtained for each week calculated. Polynomial regression models adjusted by the determination coefficient (R(2)) were created to assess the correlation between embryonic volume and gestational age (GA) and crown rump length (CRL). the intraclass correlation coefficient (ICC) was used to calculate inter-and intraobserver reproducibility. Mean embryonic volume went from 0.20 cm(3) (range 0.10 to 0.89 cm(3)) to 5.12 cm(3) (range 2.90 to 5.98 cm(3)). Embryonic volume was strongly correlated with GA (R(2) = 0.84) and with CRL (R(2) = 0.87). There was a good inter-and intraobserver reproducibility of embryonic volume with ICC = 0.999 and 0.995, respectively. Reference range for 3DUS first-trimester embryonic volume using the XI VOCAL technique were generated for pregnancies at 7 to 10 + 6 weeks, with high inter-and intraobserver reproducibility.
- ItemSomente MetadadadosSingle embryo and oocyte lipid fingerprinting by mass spectrometry(Amer Soc Biochemistry Molecular Biology Inc, 2010-05-01) Ferreira, Christina R.; Saraiva, Sergio A.; Catharino, Rodrigo R.; Garcia, Jerusa S.; Gozzo, Fabio C.; Sanvido, Gustavo B.; Santos, Luiz Fernando A.; Lo Turco, Edson G. [UNIFESP]; Pontes, Jose Henrique F.; Basso, Andrea C.; Bertolla, Ricardo P. [UNIFESP]; Sartori, Roberto; Guardieiro, Monique M.; Perecin, Felipe; Meirelles, Flvio V.; Sangalli, Juliano R.; Eberlin, Marcos N.; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de São Paulo (UNIFESP); Vitro Brasil Ltda; Universidade de São Paulo (USP)Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.-Ferreira, C. R., S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F. A. Santos, E. G. Lo Turco, J. H. F. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M. Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, and M. N. Eberlin. Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 2010. 51: 1218-1227.