Navegando por Palavras-chave "egfr"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Análise do tempo de sobrevida de doentes com adenocarcinoma colorretal esporádico pela utilização de um painel de biomarcadores de carcinogênese constituído por vegf, egfr, ki-67, p53 e bcl-2
    (Universidade Federal de São Paulo (UNIFESP), 2014-12-17) Luderer, Loreley Andrade [UNIFESP]; Matos, Delcio Matos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate the prognostic power of survival of a carcinogenesis biomarkers panel formed by p53, VEGF, Bcl-2, Ki-67, and EGFR in subjects with sporadic colorectal adenocarcinoma subjected to radical surgical treatment. Methods: 114 post-surgical subjects with colorectal adenocarcinoma were studied and followed for 3 to 5 years at Fundação Pio XII – Hospital de Câncer de Barretos. The study was conducted in paraffin-embedded tumor tissue whose slides were stained using the hematoxylin-eosin technique. The tissue microarray slides, as well as the immunohistochemical staining, were examined by two pathologists, blinded to the evaluations. The statistical analyses were conducted using mean, median, minimum, maximum, and number of valid observations for the descriptive analysis of the numeric variable, global survival. The comparison of the expression of EGFR, VEGF, Ki-67, p53, and Bcl-2 biomarkers was conducted through the Chi-square test or, when required, Fisher’s exact test. The Cox regression model was used for global survival analysis with a panel of markers and for uni and multivariate global survival analyses. Results: Isolated expression correlation results of the markers with the variables: age, differentiation degree, venous invasion, perineural invasion, TNM (I+II) x (III+IV), and survival showed statistically significant differences in the EGFR expression with venous invasion, TNM classification, and global survival; the expression of the VEGF marker has showed significant correlation with the perineural invasion; the Ki-67 marker, with age, venous invasion, and TNM; expression of p53 was significantly related with age, venous invasion, TNM, and global survival; the Bcl-2 marker did not show significant correlation with any of the variables analyzed. The survival analysis, using the markers panel, has significantly showed lesser time of survival in surgical species with 60% or more overexpression. Conclusion: Overexpression of the selected tumor markers panel is related with lesser time of survival in those suffering from sporadic colorectal adenocarcinoma subjected to radical surgical treatment.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Imunoexpressão dos biomarcadores ts, cox-2, egfr, msh6, mlh1 no adenocarcinoma colorretal e sua correlação com o grau de diferenciação tumoral e os fatores prognósticos
    (Universidade Federal de São Paulo (UNIFESP), 2013-09-25) Batista, Wilson Roberto [UNIFESP]; Matos, Delcio Matos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To study the immunoexpression of biomarkers TS, p53, COX2, EGFR, MSH6, MLH1 in patients with colorectal carcinoma, correlating with the degree of tumor differentiation and clinical prognostic factors patolólogicos. Methods: We analyzed tissues fixed in formalin and embedded in paraffin blocks of tumors from 107 patients, for immunohistochemistry by the streptavidin-biotin method using the technique of matrix arrangement of tissue samples (tissue-microarray). In the evaluation of positive markers was used categorical scores that determined the cutoff value in the percentage of stained tumor cells. Tissue expression of the proteins were correlated with the variables of degree of cell differentiation, staging, disease-free, recurrence, survival and specific mortality. We employed the Fisher exact test (Agresti, 1990) to study the association between tumor grade and TS, Cox-2, EGFR, MSH1, MSH6 and p53, and tumor staging correlated with TS, Cox-2, EGFR, MSH1, MSH6 and p53. Estimation of Kaplan-Meier (Collett, 2003), Log-rank test (Collett, 2003) and adjusting the Cox regression model (Cox, 1972) to investigate the behavior of the overall survival of patients (months) according to TS, COX-2, EGFR, MSH6, MLH1 and p53 and disease-free interval of subjects (months), according to TS, COX-2, EGFR, MSH6, MLH1 and p53. Results: The degree of tumor differentiation of individuals is not associated with TS (p = 0.138), COX-2 (p = 0.428), EGFR (p = 0.103), MSH6 (p = 0.876), MLH1 (p = 0.792) and p53 (p = 0.884). The staging is not associated with TS (p = 0.817), COX-2 (p = 0.842), EGFR (p = 0.344), MSH6 (p = 0.923) and p53 (p = 0.666). The same behavior was not observed for MLH1 (p = 0.021) in which the group of patients with stage III or IV is a higher percentage of MLH1 negative (27.4%) than in the group of patients with stage 0, I, or II (2.2%). The survival time of individuals is not related to TS (p = 0.480), COX-2 (p = 0.998), EGFR (p = 0.600), MSH6 (p = 0.318), MLH1 (p = 0.798) and p53 (p = 0.695). The disease-free interval of subjects is not related to TS (p = 0.356), COX-2 (p = 0.885), EGFR (p = 0.786), MSH6 (p = 0.178), MLH1 (p = 0.691) and p53 (p = 0.441). Conclusion: There was no correlation of the association between the degree of tumor differentiation, staging, survival time and disease-free interval with markers TS, p53, COX2, EGFR, MSH6. In advanced cases of RCC with stage III and IV, there was a higher percentage of MLH1 negative.