Navegando por Palavras-chave "dosagem de interleucinas"

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    Protocolo de estudo para a utilização da técnica seldi tof ms (surface-enhanced laser desorption/ionizatio time-of-flight mass spectrometry), no rastreamento de biomarcadores circulantes no sangue de doadores de órgãos para transplante de pâncreas
    (Universidade Federal de São Paulo (UNIFESP), 2014-12-17) Garbato, Gabriel [UNIFESP]; Linhares, Marcelo Moura Linhares [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: pancreas-kidney simultaneous transplantation is the only capable method to maintaining an euglycemia prolonged state in a type 1 diabetic patients who attend with renal failure. However, this procedure is associated with a high mortality rate due to postoperative complications and early loss of pancreatic and kidney grafts. The main cause of pancreatic graft failure, is the consequent loss cause technical dysfunction, suffering and graft thrombosis, commonly associated with clinical and biological characteristics of organ donors, as well as cellular acute rejection. By the time, donor organs criteria for selection is based on age, the serum sodium value, vasoactive drugs use and the hemodynamic changes during the capture, also the serum creatinine levels, is used to assessment of renal function. These changes characteristics are well known, that related the quality of grafts for transplantation and probably reflect a systemic metabolic and protein profile of the donor at the time of supply the organs for transplants, which has not yet been determined or associated with functioning grafts . Objectives: To present a study protocol identyfing systemic proteomic profiles of organ donors, relating it to the clinical criteria and comparing this profile to the clinical outcome of recipients of these grafts. Methods: 31 different donors, blood samples of different organs were analyzed in the state of São Paulo, subjecting them to proteomic analysis using the technique SELDI-TOF-MS (Surface Enhanced Laser Desorption-/ Ionization Time-Of-Flight Mass Spectrometry), evaluating 41 different analytes (cytokines and chemokines) standardized by HCYTOMAG KIT-60K-PX29 / PX30-HCYTMAG-60K / 60K-HCYTMAG-PX38 / PX41 HCYTMAG-60K-Milliplex®, determining in this way these donors profile, tabulating this information later to establish a large-scale 48 comparison of these profiles, with clinical data from donors and recipients and the evolution of the respective organs. Results: The IL4, IL10, IL 13, IL 1b, IL 9, IL 2 and TNF ?, showed very low doses of all 31 samples. Interleukins SDC40L, eotaxin, IP 10, MCP 1, RANTES, MDC, and IL 6, unlike the previous ones, were very high in all samples. Conclusions: This study showed that it was possible to establish a proteomic profile of donor organs that can lead to a comparative analysis with the clinical data of donors and the results of the receptors, indicating a new prevention line against adverse events, mostly related to rejection and the survival of grafts and recipients.