Navegando por Palavras-chave "conjunctiva"

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    Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation
    (Elsevier B.V., 2005-09-01) Rojas, B.; Cuhna, R.; Zafirakis, P.; Ramirez, J. M.; Lizan-garciia, M.; Zhao, T.; Foster, C. S.; Univ Complutense Madrid; Harvard Univ; Universidade Federal de São Paulo (UNIFESP); Hosp Gen Albacete
    We were interested to analyse the composition of the cellular infiltrate and adhesion molecules expression in the conjunctiva before and at least one hundred days after autologous and allogenic bone marrow transplantation (BMT) and its relation with the presence of dry eye. We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T-lymphocytes (CD3, CD4 and CD8), B-lymphocytes (CD19), macrophages (CD14), natural killer cells (NK, CD57), intercellular adhesion molecule 1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), lymphocyte function associated antigen-1 (LFA-1), very late antigen-4 (VLA-4), interleukin 2 receptor (IL2r, CD25) and HLA-DR. Our autologous recipients had no graft-versus-host disease (GVHD) but allogenic patients had chronic GVHD. After autologous BMT the conjunctiva had significantly more: (1) T lymphocytes (CD3+, CD4+, CD8+) in the epithelium; (2) CD4+ and CD 14+ cells in the stroma; and (3) VLA-4 expression in the stroma than before BMT. After allogenic BMT, the conjunctiva exhibited a significant increase of: (1) CD3+ and CD 14+ cells in the epithelium; (2) T lymphocytes (CD3+, CD4+, CD8+) and CD 14+ cells in the stroma; and (3) VLA-4 and LFA-1 expression in the stroma than before BMT. After the engraftment, the comparison between autologous and allogenic recipients revealed that: (1) there were no significant differences in adhesion molecule expression; (2) the epithelium of autologous recipients had significantly more CD3+ cells; and (3) the stroma of allogenic patients had significantly more CD3+ and CD8+ cells. Among allogenic recipients, CD14+ cells were significantly increased both in the epithelium and in the stroma of patients with signs or symptoms of dry eye in comparison with patients without ocular involvement. Additionally, those having keratoconjunetivitis sicca (KCS) had CD4/CD8 ratios significantly higher than those without KCS.In conclusion, in the conjunctiva after autologous BMT a subclinical cell mediated immune reaction seems to take place. the conjunctivitis of chronic GVHD is complex, with T cells and macrophages dramatically contributing to the process. (c) 2005 Elsevier B.V. All rights reserved.
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    Clinic-cytologic study of conjunctivochalasis and its relation to thyroid autoimmune diseases - Prospective cohort study
    (Lippincott Williams & Wilkins, 2006-08-01) Fiorentini de Almeida, Sandra Flavia; Sousa, Luciene B. de; Vieira, Luis A.; Chiamollera, Maria I.; Barros, Jeison de N.; Universidade Federal de São Paulo (UNIFESP)
    Purpose: To determine the prevalence of conjunctivochalasis in patients with immune thyroid diseases, to determine whether there is any association between the 2 diseases, and to determine cytologic study of conjunctivochalasis through the cytology impression test.Methods: A clinical prospective cohort study carried out by the External Diseases Department in the Ophthalmology Sector and the Thyroid Department in the Endocrinology Sector at Federal University of São Paulo (UNIFESP). the patients included were divided into 2 groups following these inclusion criteria: a control group of 25 patients without thyroid diseases, confirmed after clinical and laboratory examinations (thyroid hormones), or any other ocular diseases. the study group consisted of 31 patients with thyroid diseases, the diagnosis of which was confirmed by the Endocrinology Sector. the thyroidopathies included were autoimmune diseases but excluded nonautoimmune diseases. A protocol endorsed by the UNIFESP was followed, using clinical and ophthalmological history, biomicroscopy, and impression cytology.Results: Fifty-two percent of patients without thyroid diseases and 88% of patients with thyroid diseases presented with conjunctivochalasis. the risk ratio was 1.705 (Pr>chi(2)=0.0038), indicating that there is an association between them. for the impression cytology in inferior bulbar conjunctiva, there was an association between the result of the impression cytology and conjunctivochalasis (Pearson chi(2)=10.1190 Pr=0.006).Conclusion: the prevalence of conjunctivochalasis in patients with autoimmune thyroid diseases was 88%. Patients with autoimmune thyroidopathy presented higher percentages of conjunctivochalasis than the control group, confirming the association between them. the cytologic study showed the highest prevalence of abnormal surface features in eyes with conjunctivochalasis.
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    Clinical expression in 7 patients with malt lymphoma of the conjunctiva
    (Harwood Acad Publ Gmbh, 1998-01-01) Oliveira, José Salvador Rodrigues de [UNIFESP]; Colleoni,Gisele Wally Braga [UNIFESP]; Borducchi, Davimar Miranda Maciel [UNIFESP]; Rigueiro, Moacyr Pezati [UNIFESP]; Cha, S. B.; Gonzaga, Renato Luiz [UNIFESP]; Segretto, Roberto Araujo [UNIFESP]; Kerbauy, José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Problem: Lymphomas of conjunctiva are more common than is believed and immunohistologic analysis is able to distinguish benign and malignant lymphoid infiltrates. Methods: We analyzed seven cases of primary lymphoma of conjunctiva treated between November/1989 and April/1994. There were six females and one male with median age of 50 years (range 33-76 years). Results: Six patients were stage IE and one was IIE. At diagnosis, all but one had unilateral compromise of conjunctiva. All patients were classified as MALT lymphoma and six of them were positive for lambda light chain. Four patients were treated with combination of chemotherapy and bilateral radiotherapy and two received radiotherapy alone. One patient had bilateral relapse 12 months after chemotherapy alone and was successfully treated with radiotherapy. All cases treated with combined therapy achieved complete remission. Conclusion: Based on this experience, we suggest that combined treatment with chemotherapy and bilateral radiotherapy can prevent local relapse.
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    Evaluation of normal ocular bacterial flora with two different culture media
    (Canadian Ophthal Soc, 2005-08-01) Moeller, Cecilia Tobias de Aguiar [UNIFESP]; Branco, Bruno Castelo [UNIFESP]; Yu, Maria Cecília Zorat [UNIFESP]; Farah, Michel Eid [UNIFESP]; Santos, MAA; Hofling-Lima, Ana Luisa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    Background: The purpose of this study was to determine if the use of broth culture medium is efficient in investigating bacterial flora of the normal eyelid and conjunctiva.Methods: Samples from the conjunctiva and eyelid of healthy patients of various ages who were undergoing ocular surgeries were obtained and cultured at 3 periods: before topical antibiotic prophylaxis, in the postoperative period during topical antibiotic treatment, and 15 days after discontinuation of antibiotic use. Samples were inoculated into both brain heart infusion broth and blood agar plate, and the growth results of both media were analyzed.Results: Brain heart infusion broth medium showed a significantly higher bacterial growth of gram-positive cocci in most periods.The solid blood agar medium had a higher recovery of gram-positive bacilli before prophylaxis only in the older patients.Interpretation: Our results show that a more complete analysis of eyelid and conjunctival flora can be obtained using both liquid and solid media to increase the chances of isolate recovery. The inclusion of liquid media in this analysis was even more relevant in the period of concomitant use of antibiotic treatment.
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    Expression of SIRT1 in Ocular Surface Squamous Neoplasia
    (Lippincott Williams & Wilkins, 2012-07-01) Alves, Luiz Filipe de A. [UNIFESP]; Fernandes, Bruno F.; Burnier, Julia V.; Mansure, Jose J.; Maloney, Shawn; Odashiro, Alexandre N.; Antecka, Emilia; De Souza, Dominique F.; Burnier, Miguel N.; Henry C Witelson Ocular Pathol Lab; Brazilian AF Cent Hosp; Universidade Federal de São Paulo (UNIFESP); McGill Univ
    Background: the class III histone deacetylase SIRT1 is overexpressed in many malignancies and has been implicated in inactivating proteins that are involved in tumor suppression and DNA damage repair. in the current study, we examined the expression of SIRT1 in normal epithelium (NE) compared with ocular surface squamous neoplasia (OSSN) to elucidate a possible role for SIRT1 in the development or progression of this malignancy.Methods: We examined SIRT1 expression by immunohistochemistry in 47 cases of OSSN and 10 specimens of NE. Our sample included 11 benign lesions (papillomas), 25 cases of conjunctival intraepithelial neoplasia, and 11 malignant lesions of squamous cell carcinoma. the extent of staining and intensity was scored and the combined raw data were then converted to the German Immunoreactive Score.Results: Nuclear and cytoplasmic expression of SIRT1 was observed in all cases of OSSN. for the NE specimens, 50% showed negative expression and 30% weak expression, and 20% were considered significantly immunoreactive. the differential expression of SIRT1 between NE and OSSN was statistically significant (P < 0.0001). Additionally, when the staining pattern in cases of conjunctival intraepithelial neoplasia was evaluated, the staining of the more differentiated surface cells was remarkably weaker compared with the cells located closer to the basal membrane.Conclusions: SIRT1 may play an important role in the development and progression of epithelial tumors of the conjunctiva. Further research into the potential of SIRT1 as a novel therapeutic target is warranted.
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    Incidence of Melanocytic Lesions of the Conjunctiva in a Review of 10 675 Ophthalmic Specimens
    (Sage Publications Inc, 2010-02-01) Novais, Gustavo A.; Fernandes, Bruno F.; Belfort, Rubens N. [UNIFESP]; Castiglione, Enzo; Cheema, Devinder P.; Burnier, Miguel N.; McGill Univ; Henry C Witelson Ocular Pathol Lab; Universidade Federal de São Paulo (UNIFESP)
    During the study period, 10 675 human ophthalmic specimens were received at the Henry C. Witelson Ophthalmic Pathology Laboratory and Registry, McGill University, Montreal, Canada. of those, 271 were conjunctival lesions (2.5%), with 101 being classified as melanocytic: 50 (49.5%) nevi, 36 (35.6%) primary acquired melanoses, and 15 (14.9%) melanomas. After exclusion of referred cases, 85 lesions were included in the study: 44 (51.7%) nevi, 33 (38.8%) primary acquired melanoses, and 8 (9.4%) melanomas. the most prevalent location was the bulbar conjunctiva. Conjunctival melanomas were most commonly found in an older age group than primary acquired melanosis or nevi. Conjunctival nevi were subdivided into compound (32.9%), subepithelial (16.4%), and junctional (2.3%). Primary acquired melanosis were further classified into primary acquired melanosis with atypia (8.2%) and primary acquired melanosis without atypia (30.5%). Primary acquired melanoses was the predisposing lesion in 75% of the cases of melanoma. in our sample, referral bias could alter the distribution of conjunctival pigmented lesions, with a shift toward the malignant end.
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    Microbiota bacteriana aerobica conjuntival em pacientes diabeticos com hemoglobina glicada normal e alterada em duas cidades brasileira
    (Universidade Federal de São Paulo (UNIFESP), 2013-12-20) Moreno, Natalia Pimentel [UNIFESP]; Sousa, Luciene Barbosa de Sousa [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Aim: To study conjunctival aerobic bacterial microbiota of diabetic patients regarding diabetes management through glycated hemoglobin. Methods: A cross-sectional study was carried out with conjunctive smears of diabetic patients from both sexes and different ages, residents of two cities: Sorocaba (State of São Paulo) and Rio Branco (State of Acre). Control group was formed of non-diabetic patients. The sample composed of diabetic patients was considered as controlled when their glycated hemoglobin was ≤ 7%. Those whose glycated hemoglobin was > 7% were considered as non-controlled. The material obtained was sown in BHI liquid and it was seeded in culture media for aerobic bacteria (blood and chocolate agars) and bacterial growth was evaluated in a microbiology laboratory. Results: 120 eyes of 120 patients were included in this study. We observed a larger number of diabetic and non-diabetic patients aging from 61 to 70 years and also over 70 years and females. The percentage of cultures in which there was bacterial growth was greater in diabetic patients, and it was statistically significant (p <0.05). Among diabetic patients, there was no difference between bacterial growth in patients with normal and altered glycated hemoglobin and a greater tendency in diabetic patients with altered fasting glycemia. In Sorocaba, conjunctival bacterial growth was similar to Rio Branco. The most frequent microorganism in this study was Staphylococcus epidermidis, followed by Staphylococcus aureus, Proteus mirabilis and Escherichia coli. Regarding antibiotics tested in this study, there was good response of the isolated bacteria in both diabetic and non-diabetic groups. Conclusions: There was a greater conjunctival aerobic bacterial growth in diabetic patients compared to non-diabetic ones. There was no difference between patients with normal and altered glycated hemoglobin. The microorganisms found were similar
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    Transplantation of Conjunctival Epithelial Cells Cultivated Ex Vivo in Patients With Total Limbal Stem Cell Deficiency
    (Lippincott Williams & Wilkins, 2013-03-01) Ricardo, Jose Reinaldo S. [UNIFESP]; Cristovam, Priscila C. [UNIFESP]; Filho, Pedro A. N. [UNIFESP]; Farias, Charles C. [UNIFESP]; Araujo, Aline L. de [UNIFESP]; Loureiro, Renata R. [UNIFESP]; Covre, Joyce L. [UNIFESP]; Barros, Jeison N. de [UNIFESP]; Barreiro, Telma P. [UNIFESP]; Santos, Myrna S. dos [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose: To report the outcomes of transplantation of autologous conjunctival epithelial cells cultivated ex vivo (EVCAU) in patients with total limbal stem cell deficiency (LSCD).Methods: EVCAU were cultivated on denuded human amniotic membrane and transplanted in 12 eyes of 10 patients with total LSCD. We evaluated the improvement in the defined clinical parameters of LSCD (loss of corneal epithelial transparency, superficial corneal neovascularization and epithelial irregularity/recurrent epithelial breakdown), vision acuity, impression cytology, immunocytochemical analysis (CK3/CK19), and the appearance of a regular hexagonal basal layer of cells on corneal confocal microscopy. Histologic and immunohistochemical features were studied in 3 corneal buttons of patients submitted to penetrating keratoplasty after EVCAU.Results: Cultivated conjunctival epithelium formed 4 to 5 layers with the formation of basement membrane-like structures. Immunocytochemical analysis showed positivity for CK3, CK19, MUC5AC, Ki-67, P63, and ABCG2. the improvement of the clinical parameters for this treatment in our cohort was 10 of 12 (83.3%) in a mean followup time of 18.5 months (range, 15-26 months), and these eyes showed an improvement in impression cytology, immunocytochemistry, and in vivo confocal analysis. Corneal buttons showed a well-formed epithelium with 5 to 6 layers, with rare cells periodic acid-Schiff+, and positivity for CK3, CK19, P63, connexin 43, and MUC5AC.Conclusion: We demonstrated the preliminary results of transplantation of EVCAU for corneal surface reconstruction in cases with total LSCD. Future studies are needed to further assess the long-term efficacy of this procedure.