Navegando por Palavras-chave "colostrum"
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- ItemAcesso aberto (Open Access)Influência do tipo de parto sobre a concentração de imunoglobulinas A, G e M no colostro materno(Sociedade Brasileira de Pediatria, 2004-04-01) Striker, Gabriel A. J.; Casanova, Lucy D. [UNIFESP]; Nagao-Dias, Aparecida Tiemi [UNIFESP]; Hospital do Servidor Público Estadual de São Paulo; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Ceará Faculdade de Farmácia Departamento de Análises ClínicasOBJECTIVE: To investigate the association between type of delivery and immunoglobulin concentration in maternal colostrum. METHODS: We studied 82 women who were giving birth. Age was between 21 and 41 years, gestational age was 37 or more weeks and parity up to IV pregnancies. The women were in good nutritional condition and did not have any pregnancy or puerperium- associated diseases. The following aspects were also considered as inclusion criteria for the newborn: weight > 2,500 g, Apgar score > 7 in the first minute and exclusive breastfeeding until discharge from the nursery. The women were divided into three groups: A - vaginal delivery, B - caesarean section with labor and C - elective caesarean section. Colostrum was collected manually between 48 and 72h after delivery. Immunoglobulins were dosed using the ELISA technique. RESULTS: There were no differences between the three groups in terms of time of colostrum collection. The shorter the time of colostrum collection, the greater the concentration of immunoglobulin A. Primiparous women had higher concentrations of IgA and IgM in maternal colostrum than did multiparous women. The group submitted to caesarean section with labor had higher concentrations of IgA in maternal colostrum than did the normal delivery group. IgM and IgG concentrations in colostrum were not influenced by type of delivery. CONCLUSION: The occurrence of labor together with surgical stress induce higher IgA concentrations in the colostrum of women submitted to caesarean section with labor.
- ItemSomente MetadadadosInjury switches melatonin production source from endocrine (pineal) to paracrine (phagocytes) - melatonin in human colostrum and colostrum phagocytes(Blackwell Publishing, 2006-09-01) Pontes, Gerlandia N.; Cardoso, Elaine C.; Carneiro-Sampaio, Magda M. S.; Markus, Regina P.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)A large number of data show that melatonin has immunomodulatory properties and is produced by immunocompetent cells; also, some evidence suggests a 'feedback' of the activated immune system on the pineal gland. in this paper, we studied immune-pineal interactions in colostrum obtained from healthy puerperae and mothers with mastitis taking into account that, (a) melatonin levels in milk reflects pineal activity and (b) colostrum quiescent mononuclear and polymorphonuclear phagocytes from healthy mothers in culture are adequate for evaluating the ability of immunocompetent cells to produce melatonin. Here we compared the diurnal and nocturnal melatonin levels in colostrum from healthy puerperae and mothers with mastitis; this is a unique noninvasive model for determining pineal activity in the proinflammatory phase of a defense response. in addition, we determined the 'in vitro' production of melatonin by colostrum immunocompetent cells stimulated by enteropathogenic Escherichia coli or zymosan. Suppression of nocturnal melatonin rise in mothers with mastitis was highly correlated with increased tumor necrosis factor-alpha (TNF-alpha) secretion. This result, interpreted taking into account the presence of the transcription factor nuclear factor kappa B in pineal gland, suggest that the proinflammatory cytokine can inhibit nocturnal pineal melatonin production. On the other hand, stimulated, but not quiescent, immunocompetent cells secreted in the colostrum produced melatonin in vitro. in addition, this production ceases after bacteria killing. These results suggest that during the response to an injury the production of melatonin can be transiently shifted from an endocrine (pineal) to a paracrine (immunocompetent cells) source.
- ItemSomente MetadadadosPineal melatonin and the innate immune response: the TNF-alpha increase after cesarean section suppresses nocturnal melatonin production(Blackwell Publishing, 2007-11-01) Pontes, Gerlandia N.; Cardoso, Elaine C.; Carneiro-Sampaio, Magda M. S.; Markus, Regina P. [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)The nocturnal surge of melatonin is the endocrine expression of the circadian system and is essential for organizing the timing of various endogenous processes. Previous works suggest that, in the beginning of a defense response, the increase in circulating tumor necrosis factor-alpha (TNF-alpha) leads to a transient block of nocturnal melatonin production and promotes a disruption of internal time organization. in the present paper, the concentration of melatonin and cytokines [TNF-alpha, interferon-gamma (IFN-gamma), interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12] in the colostrum (postdelivery day 3) and in the milk (postdelivery days 10, 15, 20 and 30) obtained at midday and midnight from mothers who gave birth by vaginal or cesarean section were compared. the nocturnal melatonin surge observed 3 days after vaginal delivery was absent after cesarean section. IL-12 presented no daily variation in either case, while daily variations in IFN-gamma, IL-10, IL-4 and IL-5 were observed after vaginal delivery and cesarean section. On the other hand, the increase in TNF-alpha after cesarean section resulted in suppression of the nocturnal melatonin surge. Daily variation of IL-2 was only observed after recovery of the nocturnal melatonin surge, 30 days after cesarean section. the present paper supports the hypothesis of a cross-talk between the pineal gland and the immune system, which could represent a putative immune-pineal axis.