Navegando por Palavras-chave "breast carcinoma"
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- ItemSomente MetadadadosAntigen retrieval methods and estrogen receptor immunoexpression using ID5 antibody: A comparative study(Westminster Publ Inc, 2005-10-01) Neves, J. I.; Begnami, M. D.; Arias, V; Santos, G. C.; AC Camargo Hosp; Samaritano Hosp; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Long-term formalin tissue fixation results in antigen masking, probably through aldehydic linkage between proteins and fixative molecules. Immunohistochemistry results depend on the type of the detection procedure and the type of antibody used for the reaction. Considering the difficulty in working with estrogen receptor (ER) antibodies and the lack of standardization of the antigen retrieval methods, we quantified the immunoexpression of ER using the ID5 antibody and a standard streptavidin-biotin detection procedure retrieving with microwave even, steamer, pressure cooker, and water bath in a set of SBR grade 2 invasive breast carcinomas. Pressure-cooking provided the best results. No significant differences were observed in using the other methods. Pressure-cooking should be recommended as the method of choice for standardization of the ER immuohistochemical reaction.
- ItemSomente MetadadadosBreast Carcinoma-associated Fibroblasts Share Similar Biomarker Profiles in Matched Lymph Node Metastasis(Lippincott Williams & Wilkins, 2016) Mundim, Fiorita Gonzales Lopes [UNIFESP]; Pasini, Fatima S.; Nonogaki, Suely; Rocha, Rafael M.; Soares, Fernando A.; Brentani, Maria M.; Logullo, Angela Flavia [UNIFESP]This study sought to understand the role of breast carcinoma-associated fibroblasts in the progression of cancer cells into lymph nodes. We compared fibroblasts of primary tumors and matched the involved lymph nodes to select fibroblast activation markers, namely -smooth muscle actin (-SMA), S100A4, and vimentin, as well as to determine the frequency of transforming growth factor 1, a pleiotropic cytokine that induces the differentiation of fibroblasts to myofibroblasts, and its downstream effectors: CXCR4 and p-AKT. We disposed samples of 80 primary invasive ductal carcinomas and matched the involved lymph nodes from 43 cases into 3 tissue microarrays, and analyzed stromal and tumor epithelial cells separately by immunohistochemistry. Control uninvolved lymph nodes were analyzed by whole-tissue sections. Cancer-associated fibroblast in lymph nodes with macrometastasis expressed similar profiles of vimentin, -SMA, and S100A4 as those found in primary tumors. Cancer-associated fibroblast were uniformly estrogen receptor, progesterone receptor, HER-2, Ki-67, and p53 negative, but expressions of transforming growth factor 1 (TGF1), CXCR4, and p-AKT staining (62.3%, 52.4%, 65%, respectively) were equivalent between primary and lymph node metastasis (LNM) fibroblasts. A significant coexpression of TGF1 with p-AKT and CXCR4 in LNMs suggested the involvement of these proteins with TGF1 signaling. These biomarkers, including -SMA and S100A4, were negative in fibroblasts of cancer-free lymph nodes, with the exception of vimentin. Our finding that expressions of biological markers were similar in fibroblasts of the primary tumors and in matched LNMs, but were absent in cancer-free lymph nodes, supports the assumption that the lymph node stroma mimics the microenvironment observed in primary tumors.
- ItemSomente MetadadadosGerbB-2 expression is a better predictor for survival than galectin-3 or p53 in early-stage breast cancer(Professor D A Spandidos, 2007-07-01) Logullo, Angela Flavia [UNIFESP]; Lopes, Andréa Braga de Godoy; Nonogaki, Suely; Soares, Fernando Augusto; Mourão Netto, Mario; Nishimoto, Inês Nobuko; Brentani, Maria Mitzi [UNIFESP]; Universidade de São Paulo (USP); Hosp Canc; Inst Adolfo Lutz Registro; Universidade Federal de São Paulo (UNIFESP)The definition of high risk patients with early stage breast cancer is still controversial. We evaluated the ability of galectin-3, c-erbB-2 and p53 immunohistochemical expression to predict recurrence and survival in a homogeneous set of 92 patients with T1N0M0 ductal carcinoma with a long-term follow-up. In normal breast tissue, the epithelial and fibroblast components were positive for galectin-3 mostly showing nuclear and cytoplasmic reactivity. At the tumor epithelial component, galectin-3 expression was found in 46.7% of the samples with a predominant cytoplasmic staining. Similar results were presented by concurrent in situ lesions. Tumor stromal fibroblasts maintained positivity in 70 out of 92 cases (76%). We found expression of p53 in only 16 cases (17.4%), and c-erbB-2 in 17 (18.48%). A marginal association was found between co-expression of p53 and galectin-3 (p=0.055) and a significant correlation between p53 accumulation and c-erbB-2 expression (p=0.009). There was no significant association between (galectin-3 protein expression with disease-free survival or overall survival. C-erbB2 and p53 expression correlated with recurrence (p=0.002, p=0.02; respectively). Diminished overall survival at 10 years was associated with c-erbB-2 (p=0.010), but marginally with p53 expression (p=0.076). Epithelial galectin-3 expression cannot be considered a prognostic factor for patients with T1N0M0 breast cancer, p53 seems to be of minor relevance and c-erbB-2 expression was the best discriminator and may be a marker for aggressive clinical behavior in patients with early stage breast cancer.
- ItemSomente MetadadadosQuantification of angiogenesis induced in rabbit cornea by breast carcinoma of women treated with tamoxifen(Wiley-Blackwell, 2005-05-01) Da Silva, B. B.; Da Silva, R. G.; Borges, U. S.; Filho, MAGD; Pimentel, ICC; Gebrim, L. H.; Simoes, M. D.; Baracat, E. C.; Fed Univ Piaui; Universidade Federal de São Paulo (UNIFESP)Background: the aim of this study was to evaluate the effect of tamoxifen on angiogenesis induced in rabbit cornea by breast curcinoma from post-menopausal women.Methods: Thirteen post-menopausal women with operable, estrogen receptor-positive breast carcinomas, treated with 20 mg of tamoxifen daily for 30 days prior to undergoing definitive surgery, participated in this study. Twenty-six virgin female New Zealand white rabbits, 3-4 months old, weighing approximately 2.5 kg each, were divided into two groups of 13 animals and received corneal implants of tumor either pre- or post-tamoxifen treatment. After 10 days, tie animals were sacrificed, the region of the cornea between the tumor implant and the limbus was removed and quantification of angiogenesis was carried out by eualuating the entire hematoxylineosin stained slide, using a 10x objective lens (100x magnification). Student t-test was used in the statistical analysis of the data and statistical significance was established at P < 0.05.Results: Mean microvessel count was 106.8 +/- 5.9 pre-tamoxifen treatment and 54 +/- 5.6 post-treatment. According to Student t-test, there was a significant reduction in mean microvessel density following treatment with tamoxifen (P < 0.001).Conclusions: Rabbit cornea proved to be an interesing experimental model for the quantification of angiogenesis. Tamoxifen, when administered for 30 days to postmenopausal women with breast cancer, significantly inhibited angiogenesis induced by tumor fragments in rabbit cornea. (C) 2005 Wiley-Liss, Inc.