Navegando por Palavras-chave "amniotic fluid"

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    Effects of Topical Human Amniotic Fluid and Human Serum in a Mouse Model of Keratoconjunctivitis Sicca
    (Lippincott Williams & Wilkins, 2012-04-01) Quinto, Guilherme G. [UNIFESP]; Camacho, Walter; Castro-Combs, Juan; Li, Li; Martins, Suy Anne R.; Wittmann, Priscila; Campos, Mauro [UNIFESP]; Behrens, Ashley; Johns Hopkins Univ; Universidade Federal de São Paulo (UNIFESP)
    Purpose: To compare the effects of topical human amniotic fluid (HAF), topical human serum (HS), and topical artificial tears in a mouse model of dry eye.Methods: Thirty C57BL/6 mice were divided into 3 treatment groups: HAF, HS, and preservative-free artificial tears. Dry eye was induced by an injection of botulinum toxin B (BTX-B) into the lacrimal gland. Tear production and ocular surface fluorescein staining were evaluated in each mouse at 6 time points during a 4-week period. Goblet cell density was assessed in stained histological sections. Apoptotic keratocytes were evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling test assay.Results: A significant decrease in tear production was observed 3 days after BTX-B injection in all groups. At week 1, the HAF and HS groups had improved tear production compared with the control group (P < 0.001 and P = 0.003, respectively). HAF had a significantly improved fluorescein staining score compared with the HS (P = 0.043) and control (P = 0.007) groups at week 2. Goblet cell density was significantly decreased in the control group compared with the HAF and HS groups (P < 0.001). No difference in the amount of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive keratocytes was observed among the groups.Conclusion: HAF was superior to HS and artificial tears for improving corneal staining within 2 weeks of therapy in this induced mouse model of keratoconjunctivitis sicca. Clinical studies are needed to ascertain the benefits of these therapies in patients with ocular surface disorders associated with dry eye.
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    Embolia pulmonar por líquido amniótico: relato de caso e revisão da literatura
    (Associação de Medicina Intensiva Brasileira - AMIB, 2007-06-01) Almeida, Edmilton Pereira De; Almeida, Maria Antônia Campos [UNIFESP]; Amaral, Lígia Menezes Do; Soares Júnior, Cleber; Haddad, Márcia Abudd; Rodrigues, Lúcia Lopes; Hospital Monte Sinai UTI Adulto; Universidade Federal de São Paulo (UNIFESP); AMIB
    BACKGROUND AND OBJECTIVES: Amniotic fluid embolism is a rare complication of pregnancy whose physiopathology is not completely known and still not frequently remembered by intensive care physicians and obstetricians. The main aim of this case report was to emphasize the need of better knowledge of this disease by physicians. CASE REPORT: A 15 years old, primigravida, 35th week of gestation was admitted in our intensive care unit (ICU) with a sudden respiratory distress. Soon after admission she was intubated and put on mechanical ventilation. After stabilization she was transferred to the operating room and a cesarean section was undertaken. During the operation, instead of fluid reposition with crystalloids in large amounts, her hemodynamic status deteriorated and it was necessary to use vasoactive drugs. After three days on mechanical ventilation she was successfully weaned and discharged from the intensive care unit (ICU) after 6 days. She and her baby were discharged from hospital on13th day of hospitalization. CONCLUSIONS: Amniotic fluid embolism is a rare and catastrophic complication of pregnancy, at least in its classic presentation. It is characterized by acute respiratory distress, hemodynamic compromise and coagulopathy that occur during or within 30 min after labor. Diagnosis is by exclusion of other conditions of pregnancy or diseases that can be aggravated during pregnancy such as eclampsia, sepsis, pos-partum cardiomyopathy, anaphylaxis, pulmonary thromboembolism, transfusion reactions, anesthetic complications and mitral stenosis. There is growing evidence that we have a spectrum of manifestation which can be more common that in the classic ones. The treatment is supportive of vital functions, such as mechanical ventilation, fluid reposition, vasoactive drugs and fresh frozen plasma as necessary. The intensive care physicians and obstetricians should be aware of this disease in order to make early diagnosis and prompt treatment.