Navegando por Palavras-chave "alfa-Tocoferol"

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    Efeitos histologicos e bioquimicos sericos do alfa-tocoferol na lesao de isquemia e reperfusao em membro pelvico de ratos
    (Universidade Federal de São Paulo (UNIFESP), 2004) Silva, Marcelo Gomes da [UNIFESP]
    Objetivo: avaliar a acao protetora do Alfa-tocoferol na lesao de isquemia e reperfusao em membro pelvico de ratos. Metodos: 30 ratos foram distribuidos aleatoriamente, em tres grupos experimentais, com 10 animais cada: Grupo I Grupo de controle sem isquemia ou reperfusao; Grupos II e III - quatro horas de isquemia e duas horas de reperfusao atraves do clampeamento da aorta abdominal distal. Os animais do grupo II foram tratados com solucao salina, e aqueles do grupo III, tratados com alfa-tocoferol por via endovenosa. Parametros estudados: biopsias do musculo solear, dosagens creatina fosfoquinase, desidrogenase lactica, ureia, creatinina, sodio, potassio, lactato, calcio e hemogasometria arterial. Resultados: os resultados das biopsias dos musculos soleares (edema) estudados atraves da microscopia optica, nao apresentaram significancia entre os tres grupos estudados. As variaveis inflamacao e necrose foram ausentes e portanto nao analisaveis estatisticamente. Em relacao as dosagens de ureia, creatinina, calcio, sodio, desidrogenase latica, pH, p02, pC02, nao foram significantes em todos os grupos estudados. Observamos que os niveis de potassio, lactato, creatina fosfoquinase e bicarbonato, apresentaram resultados significantes entre os grupos estudados. Conclusao: o tratamento com Alfa-tocoferol do ponto de vista bioquimico serico atenuou as lesoes metabolicas na sindrome de isquemia e reperfusao neste modelo experimental
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    Histological and biochemical serum effects of alpha-tocopherol on ischemia/reperfusion-related injuries induced in the pelvic limb of rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2005-10-01) Silva, Marcelo Gomes da [UNIFESP]; Castro, Aldemar Araujo [UNIFESP]; Ramos, Eduardo Antonio Gonçalves; Peixoto, Ediriomar; Miranda Junior, Fausto [UNIFESP]; Pitta, Guilherme de Benjamin Brandão; Costa, Regina de Faria Bittencourt da [UNIFESP]; Juliano, Yara [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Faculty of Technology and Science; University of Sciences of Alagoas; Federal University of Bahia; Oswaldo Cruz Foundation Histopathology Unit; Bahiana School of Medicine and Public Health Department of Experimental Surgery and Operative Technique; Governor Lamenha Filho University Foundation for Science and Health of Alagoas Department of Surgery; Heliopolis Hospital Vascular Surgery Service
    PURPOSE: To evaluate the protective action of alpha-tocopherol in ischemia/reperfusion injuries of pelvic member of rats. METHODS: Thirty adult male rats of the Wistar strain were randomized into three experimental groups of 10: Group I - control group with no ischemia or reperfusion. Groups II and III - four hours of ischemia and of hours of reperfusion by means of clamping of the infrarenal aorta. The animals of Group II were treated with saline and those of Group III were treated with i.v. alpha-tocopherol (50 mg/kg). Parameters studied were biopsies of the soleus muscle, dosing of creatine phosphokinase, lactate dehydrogenase, potassium, calcium and arterial blood gasometry. RESULTS: The results of biopsies of the soleus muscles studied by optical microscopy, were not significant in terms of presence of edema among the three groups studied. Variables inflammation and necrosis were not observed, therefore cannot be statistically analyzed. As to dosing of calcium and lactate dehydrogenase, the pH, pO2 and pCO2 values were not significant for all groups studied. We observed that the levels of potassium (Group II > Group I, Fcalculated = 5.84; Fcritical = 3.33), creatine phosphokinase (Group II > Groups I and III, Hcalculated = 13.92; Hcritical = 5.99) and bicarbonate (Groups I and III > Group II, Hcalculated = 11.98; Hcritical = 5.99) presented significant results among groups. CONCLUSION: From the serum biochemical perspective, the treatment with alpha-tocopherol has attenuated the metabolic injuries in the ischemia/reperfusion syndrome in this experimental model.
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    Modificações induzidas pela radiação gama em células da medula óssea de camundongos tratados com alfa-tocoferol
    (Universidade Federal de São Paulo (UNIFESP), 2003) Moraes, Andrea Aparecida Fátima Souza [UNIFESP]; Ferreira, Alice Teixeira [UNIFESP]
    O autor so fez o abstract:The bone marrow is a highly radiosensitive organ affected by accidental exposures to ionizing radiation and during radiotherapy. The effects induced by y radiation depend on the tissue absorbed dose and cell repair capacity. Apoptosis is a mode of cell death that results in the deletion of cells in a normal tissue. It also occurs naturally in some neoplasias. The process is induced by radiation and chemotherapic agents. The morphological features are: condensation and fragmentation of the nuclear chromatin and cell fragmentation, producing the apoptotic bodies. The biochemical analysis shows the DNA fragmentation in a ladder pattern. It has been shown that genes are involved in the induction as well as in the inhibition of apoptosis. It has also been shown that calcium is involved in the process of apoptosis depending on cell type. The interest in apoptosis has expanded since its importance in oncology was recognized. Indeed, the early tumor response to radiation and the high radiosensitivity of bone marrow are related to apoptosis. The a-tocopherol (Vitamin E) has been widely used due to its characteristical protection against oxidative stress in various biological systems. Vitamin E is known as a sinergetic antioxidant and it is used to protect the organisms from the effects of physical and chemical agents. Vitamin E restricts the propagation of the chain reaction of reactive oxygen species (ROS), induces a natural protection on biological membranes against radiation injuries that can lead the cells to death by reproductive falency and apoptosis. This work examined the effects of gamma radiation in the bone marrow cells using the a-tocopherol as a radioprotector. Mice (C57BU10) were subdivided into 4 groups: GC (Control), GI (Irradiated), GC-11 (Control+VitE), and GI-11 (irradiated+VitE). GC-11 and GI-11 were treated intraperitoneally with VitE (40mg/Kg)/14days. The animals of GI and GI-11 were submitted to gamma irradiation in a telecobalt therapy source (Alcyon RI), with rate of 0,61 cGy/min. The mice were whole body irradiated with the dose of 7Gy. A flow cytometric morphological analysis were performeda(au)