Navegando por Palavras-chave "adherence pattern"

Agora exibindo 1 - 2 de 2
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E-coli and chain-like adherent E-coli isolated from children with and without diarrhoea, in Londrina, Brazil
    (Elsevier B.V., 2000-09-15) Gioppo, Nereida MR; Elias Junior, Waldir Pereira [UNIFESP]; Vidotto, Marilda C.; Linhares, Rosa E.; Saridakis, Halha O.; Gomes, Tania Aparecida Tardelli [UNIFESP]; Trabulsi, Luiz R.; Pelayo, Jacinta S.; Universidade Estadual de Londrina (UEL); Inst Butantan; Universidade Federal de São Paulo (UNIFESP)
    A total of 919 Escherichia coli isolates from 125 children with diarrhoea (cases) and 98 controls were assayed for adherence to HEp-2 cells. Localised adherence was found only in isolates from cases. Diffuse, aggregative (AA), chain-like adherence (CLA) and variants of the AA pattern were found in both cases and controls. the AA isolates were tested for gene sequences associated with enteroaggregative E. coli (EAEC). Only 25% of the isolates hybridised with the EAEC probe, and the aafA, astA and pet gene sequences were found in 7.9%, 44.7% and 7.9% of the isolates, respectively. the aggA gene was not found, although 7.9% were positive for aggC. the CLA isolates reacted with the EAEC probe (55.6%),nd the aggC, astA and pet gene sequences were found in 66.7%, 33.3% and 11.1%, respectively. the aggR (55.6%), aspU (55.6%), shf(33.3%) and she (22.2%) genes were also found in CLA isolates. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Virulence properties of Escherichia coli O111:H12 strains
    (Wiley-Blackwell, 1997-01-01) Monteiro-Neto, Valério; Campos, Leila Carvalho; Ferreira, Antonio José Piantino; Gomes, Tania Aparecida Tardelli [UNIFESP]; Trabulsi, Luiz Rachid; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    Seventeen Escherichia coli O111:H12 strains isolated from the feces of children with acute diarrhea were studied with regard to their adherence properties and other virulence characteristics. All strains showed an aggregative adherence pattern to HEp-2 cells and agglutinated bovine and sheep red cells in the presence of mannose. These strains did not have gene sequences homologous to the aggregative adherence fimbria I gene and did not react with any of the DNA probes used to detect other virulence genes in enteropathogens. With one exception, the O111:H12 strains did not induce fluid accumulation in the rabbit ileal loop assay, although 16 of the strains had the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) gene sequences. A 60-70 MDa plasmid was present in 16 of the strains studied. We conclude that the O111 :H12 serotype, one of the first E. coli identified in infantile diarrhea, belongs to the enteroaggregative E. coli category but the genes encoding its adherence phenotype are distinct from those previously described.