Navegando por Palavras-chave "Vocal fold"
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- ItemSomente MetadadadosAugmentation of the Porcine Vocal Fold Using Autologous Composite Cervical Fascia and Fat Graft. Comparison Between the Transmuscular and Submuscular Approaches(Elsevier B.V., 2011-09-01) Lins Perazzo, Paulo Sergio [UNIFESP]; Sarvat, Marcos Andre [UNIFESP]; Amorim Filho, Francisco de Souza [UNIFESP]; Lima Pontes, Paulo Augusto de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objectives. To assess the integration of an autologous composite fascia and fat graft implanted into the lamina propria of a porcine vocal fold using two different approaches.Study Design. An experimental prospective study on the porcine larynx was conducted at a tertiary research institution.Methods. An external cervical approach was used to expose the thyroid cartilage of 24 healthy minipigs under general anesthesia. the composite fascia/fat graft was implanted through two distinct approaches, transmuscular and submuscular. Animals were sacrificed at 7, 30, 90, and 180 days for macroscopic and histological study of the larynx. Graft integration and local inflammatory response were studied.Results. the survival rate of the experimental model was 100% and all animals had local inflammatory response to the surgical procedure. Only 41.7% of the grafts placed inside the thyroarytenoid (TA) muscle fibers were identified postmortem, whereas 83.3% of the submuscular grafts remained intact.Conclusions. Graft incorporation was better and there was less inflammation when the architecture of the TA muscle fibers was preserved. Graft extrusion was observed in the most of the cases where it was placed inside the muscle.
- ItemSomente MetadadadosBacterial Cellulose as Laryngeal Medialization Material: An Experimental Study(Elsevier B.V., 2011-11-01) Souza, Flavia Coelho de; Olival-Costa, Henrique; Silva, Leonardo da; Pontes, Paulo A. [UNIFESP]; Penteado Lancellotti, Carmen Lucia; FCMSCSP; Santa Casa São Paulo FCMSCSP; Universidade Federal de São Paulo (UNIFESP)Hypothesis. the use of a material made of bacterial cellulose with the aim of obtaining vocal fold medialization has not hitherto been fully investigated. Although the material has been tested in other animal models, the evaluation did not include the larynx; hence, situations, such as tissue reaction, material absorption, and extrusion, need to be addressed to evaluate its usefulness as a material for laryngeal reconstruction.Objective. To evaluate the medialization, tissue response, and healing of rabbit vocal folds, after the implantation of a membrane of bacterial cellulose.Study Design. Experimental study.Methods. A total of 32 :rabbits were used, two of which were used to check out the adequacy of the implant location. the animals were followed for 4 months and grouped according to follow-up times of 2, 4, and 16 weeks. All test animals received an implant of bacterial cellulose in one vocal fold and the injection of distilled water in the other, both performed by videoendoscopic cervicotomy. At the end of the follow-up, the presence of inflammatory and medial displacement was evaluated.Results. No statistically significant difference in the inflammatory parameters between the study and control vocal folds or among follow-up times was found. All animals receiving cellulose presented medial displacement of vocal folds, and all retained this material at the implant site up to study endpoint.Conclusion. Bacterial cellulose is a useful material for laryngeal medialization, showing no signs of rejection or absorption.
- ItemAcesso aberto (Open Access)Detecção de receptor de ácido hialurônico em prega vocal humana por método imunohistoquímico(ABORL-CCF Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial, 2008-04-01) Barbosa, Luiz Henrique Fonseca [UNIFESP]; Ramos, Hugo Valter Lisboa [UNIFESP]; Neves, Luciano Rodrigues [UNIFESP]; De Biase, Noemi Grigoletto [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Pedroso, José Eduardo de Sá [UNIFESP]; Pontes, Paulo Augusto de Lima [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); UFBAHyaluronic acid receptor is a glycoprotein of the plasmatic membrane, and the CD44 is its representative, expressed in many cell types where it has the task of cell adhesion. AIM: the goal of the present experimental study is to investigate the possibility of using immunohistochemistry to identify the distribution of hyaluronic acid along the vocal fold. MATERIALS AND METHODS: We resected the normal vocal folds from a normal 23 year-old male black individual. The slides were analyzed by means of a histomorphometric study, comparing the color intensity in the superficial, middle and deep layers of the lamina propria. In the silanized slides we used immunohistochemistry, and evaluated the slides under light microscopy with 40x magnification, and the color changed to brown when there was a reaction with the receptor for hyaluronic acid. RESULTS: Immunohistochemical findings showed the presence of hyaluronic acid receptors in the epithelium covering the vocal fold, being more concentrated in the central region of the vocal fold. CONCLUSION: immunohistochemistry, used to assess the distribution of hyaluronic acid receptors in the central portion of the vocal fold, proved it to be present in the vocal fold epithelium and it prevailed in its middle third.
- ItemAcesso aberto (Open Access)Imunohistoquímica como método de estudo das fibras elásticas em prega vocal humana(ABORL-CCF Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial, 2005-08-01) Ramos, Hugo Valter Lisboa [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Pontes, Paulo Augusto de Lima [UNIFESP]; Neves, Luciano Rodrigues [UNIFESP]; Barbosa, Luiz Henrique Fonceca; De Biase, Noemi Grigoletto [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)AIM: Verify the use of immunohistochemistry as a method to measure all forms of elastic fibers at human vocal folds. STUDY DESIGN: transversal cohort. MATERIAL AND METHOD: We collected vocal folds following these criterion: age between 25 and 40, Caucasian men, dead by gun shot, within 12 hours of death, without instrumentation of the larynx or suspicion of neck injury and without mucosal lesions noted by microscopy. Ten vocal folds were collected and one, of a man aged 28 years, was selected to study. The vocal fold was transversely cut in 9 regions and in each segment three slides were made. These slides were stained by Verhoeff and Weighert's resorcin-fuchsin and used for immunohistochemistry. The elastic compound was measured by colorimetric software analysis. RESULTS: In Verhoeff and Weighert's resorcin-fuchsin, the intermediate and deep layer showed values higher than those of the superficial layer. The amount of tropoelastin identified by the antibody at the superficial layer was close to those of intermediate and deep layer. CONCLUSION: Immunohistochemistry is a method that can identify and measure all forms of elastic fibers at human vocal fold.
- ItemAcesso aberto (Open Access)Prevalência das alterações estruturais mínimas da laringe: influência sobre o conceito de prega vocal normal(Universidade Federal de São Paulo (UNIFESP), 2015-10-28) Moraes, Bruno Teixeira de [UNIFESP]; De Biase, Noemi Grigoletto [UNIFESP]; http://lattes.cnpq.br/3156326658988323; http://lattes.cnpq.br/6471177348055590; Universidade Federal de São Paulo (UNIFESP)Introdução: Sulcos vocais, cistos epidermoides, pontes de mucosa e microdiafragmas laríngeos constituem um grupo de alterações morfológicas encontradas nas pregas vocais designado como alterações estruturais mínimas de cobertura da laringe. Há uma vasta discussão na literatura a respeito da origem e impacto vocal destas entidades com indefinição se elas representam variações anatômicas pertencentes ao campo da anatomia ou se consistem em anormalidades congênitas ou adquiridas pertencentes ao campo da patologia. A utilização de um método padrão ouro para fins de diagnóstico e prevalência destas alterações, numa população avaliada independente de apresentar queixa vocal, contribuiria muito no conhecimento de sua etiologia, fisiopatologia e impacto clínico na saúde do indivíduo. Objetivos: Determinar a prevalência de alterações estruturais mínimas de cobertura da laringe em uma população e caracterizá-las sob parâmetros morfológicos e epidemiológicos. Método: Estudo observacional do tipo transversal. Um total de 147 pacientes foram submetidos à anestesia geral para cirurgia de sítio extralaríngeo com indicação de procedimento alheia ao estudo e independente da presença de queixa vocal. Em seguida, realizou-se a detecção e caracterização morfológica de alterações estruturais mínimas e outros achados por meio de microlaringoscopia de suspensão com inspeção e palpação laríngea sem intervenção cirúrgica relacionada a este procedimento. Os achados laríngeos foram cruzados com variáveis do questionário de características clínicas e demográficas aplicado nestes indivíduos. Resultados: Em amostra com maioria de indivíduos sem queixas vocais (95,9%) foi identificada uma prevalência de alterações estruturais mínimas de 21,1% nesta população. Destes, 5,4% dos indivíduos apresentaram microdiafragma laríngeo, 16,3% sulcos vocais e 4,1% cisto epidermoide. Não foram encontrados sulco-bolsa ou ponte de mucosa nesta amostra. A identificação destas alterações ocorreu majoritariamente após os 10 anos de idade (período de maturação da lâmina própria) sem influência de gênero, abuso vocal ou afecções de via aérea superior. Entre as alterações com apresentação múltipla, houve associação principalmente entre sulcos vocais. Também observou-se na amostra nódulo vocal em 12,9% dos casos, vasculodisgenesia em 1,4% e ectasia vascular em 2%. Conclusões: A prevalência de alterações estruturais mínimas de coberturada laringe nesta população predominantemente assintomática do ponto de vista vocal foi de 21,1% com identificação de sulcos vocais, cistos epidermoides e microdiafragmas laríngeos. A idade foi a única característica clínica ou demográfica associada a estas alterações.