Navegando por Palavras-chave "Vagina"

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    Analysis of the ki-67 index in the vaginal epithelium of castrated rats treated with tamoxifen
    (Hospital clinicas, univ sao paulo, 2016) Nery-Aguiar, Afif Rieth; Aguiar, Yousef Qathaf; Conde Junior, Airton Mendes; Alencar, Airlane Pereira; Tavares, Cleciton Braga; Lopes-Costa, Pedro Vitor; Nazario, Afonso Celso [UNIFESP]; da Silva, Benedito Borges
    OBJECTIVES: Vaginal atrophy and breast cancer are common conditions in postmenopausal women and tamoxifen is the standard endocrine treatment for hormone-sensitive tumors. The present study aimed to assess the effect of tamoxifen on Ki-67 protein expression in the vaginal epithelium of castrated rats. MATERIAL AND METHODS: Forty Wistar-Hannover adult, virgin, castrated rats were randomly divided into two groups, group I (control, n= 20) and group II (tamoxifen, n= 20), receiving 0.5 ml of propylene glycol and 250 mu g of tamoxifen diluted in 0.5 ml of propylene glycol, respectively, daily by gavage for 30 days. On the 31st day, the rats were euthanized and their vaginas were removed and fixed in 10% buffered formalin for the immunohistochemical study of Ki-67 protein expression. Data were analyzed by the Levene and Student's t tests (p<0.05). RESULTS: The mean index of Ki-67 expression in the rat vagina of groups I and II was 4.04 +/- 0.96 and 26.86 +/- 2.19, respectively (p<0.001). CONCLUSIONS: According to the results of the present study, tamoxifen, at the dose and treatment length used, induced a significant increase in the cell proliferation of the vaginal mucosa in castrated rats, as evaluated by Ki-67 protein expression.
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    Aspectos histomorfométricos e imuno-histoquímicos da vagina de ratas tratadas com isoflavonas e/ou estrogênios
    (Universidade Federal de São Paulo (UNIFESP), 2008) Carbonel, Adriana Aparecida Ferraz [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]
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    Aspectos ultra-estruturais do corpo lúteo e do epitélio vaginal de ratas albinas no 15º dia de pós-parto tratadas por metanosulfonato de 2-bromo-alfa-ergocriptina (bromocriptina)
    (Universidade Federal de São Paulo (UNIFESP), 1988) Ennes, Danilo Kfouri [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]
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    Avaliação das respostas clínica e inflamatória sistêmicas na colec istectomia pelas vias de acesso portal único umbilical, NOTES transvaginal, laparoscópica e laparotômica: estudo experimental em suínos
    (Universidade Federal de São Paulo (UNIFESP), 2013) Caetano Junior, Elesiario Marques [UNIFESP]; Linhares, Marcelo Moura [UNIFESP]; http://lattes.cnpq.br/0461653687573670; http://lattes.cnpq.br/1075096529163235; Universidade Federal de São Paulo (UNIFESP)
    Objetivo: Avaliar as respostas clinica e inflamatoria do trauma cirurgico provocado pela colecistectomia pela vias de acesso portal unico umbilical, NOTES transvaginal, laparoscopia e laparotomia. Metodo: Vinte e oito femeas suinas foram distribuidas em quatro grupos de sete animais e submetidas a colecistectomia pelas vias portal unico umbilical, laparoscopica, laparotomica e NOTES exclusivamente transvaginal. Um grupo com cinco animais serviu de controle (Sham). Os animais foram monitorados e avaliados no transoperatorio quanto aos tempos anestesico e cirurgico, bem como a presenca de complicacoes. No pos-operatorio, foram avaliados quanto aos tempos de deambulacao e alimentacao, e a presenca de intercorrencias clinicas. Dosagens de procalcitonina, PCR e IFN- foram feitas no pre e pos-operatorio imediato, 2º e 7º dias de pos-operatorio, quando os animais foram sacrificados e necropsiados. Resultados: Todos os procedimentos foram realizados com sucesso conforme proposto em cada grupo. Houve apenas casos de complicacoes pequenas no transoperatorio, do tipo perfuracao da vesicula biliar e sangramento no leito hepatico em todos os grupos. O tempo anestesico-cirurgico foi maior no grupo NOTES vaginal em relacao aos demais grupos (p<0,001). Os tempos de recuperacao pos-anestesica, deambulacao, alimentacao e evolucao clinica foram semelhantes em todos os grupos. Os niveis de procalcitonina, PCR e IFN- tambem foram semelhantes entre eles. Nos achados da necropsia, apenas aderencias foram encontradas, sem diferenca entre os grupos. Conclusao: A colecistectomia com portal unico mostrou-se efetiva e semelhante a via laparoscopica e convencional. As vias de acesso portal unico umbilical e NOTES totalmente transvaginal mostraram-se factiveis e seguras, quando comparadas as vias de acesso laparoscopica e laparotomica. O tempo cirurgico foi maior na via NOTES em relacao as vias laparoscopica, portal unico umbilical e laparotomica. Nao houve diferenca significante da resposta inflamatoria entre os grupos estudados com base nos niveis sericos de PCR, IFN- e procalcitonina.
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    Avaliação do impacto da correção cirúrgica de distopias genitais sobre a função sexual feminina
    (Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2007-10-01) Prado, Daniela Siqueira [UNIFESP]; Arruda, Raquel Martins [UNIFESP]; Figueiredo, Raquel Cristina De Moraes; Lippi, Umberto Gazi; Girão, Manoel João Batista Castello [UNIFESP]; Sartori, Marair Gracio Ferreira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Hospital do Servidor Público Estadual de São Paulo Francisco Morato de Oliveira Serviço de Ginecologia e Obstetrícia; Hospital do Servidor Público Estadual de São Paulo Francisco Morato de Oliveira; Hospital do Servidor Público Estadual de São Paulo Francisco Morato de Oliveira Setor de Uroginecologia e Cirurgia Vaginal
    PURPOSE: to identify the impact of pelvic reconstructive surgery on female sexual function, as well as the changes in vaginal anatomy, and to detect possible correlations between them. METHODS: a prospective, descriptive study, including 43 sexually active women with genital dystopy, undergoing surgery for pelvic organ prolapse, conducted between October 2004 and September 2006. The women completed the same multiple-choice questionnaire regarding sexual function, and analogic scales to quantify the degree of desire, arousal and satisfaction, and were clinically assessed using the pelvic organ prolapse quantification (POP-Q) staging system, before the surgery and three and six months after it. Statistical analysis was performed through the Bowker test for symmetry, Wilcoxon test, Student t test, chi2 and analysis of variance (ANOVA) as appropriate, with statistical significance set at 5% (p<0.05). RESULTS: all 43 women completed the follow-up at three and six months after the surgery, but two of them lost their partners after the surgery. Quality of sexual life improved significantly (p=0.03). Symptoms such as dyspareunia (25.6% before versus 17.1% after surgery), discomfort (27.9 versus 0%), embarrassment (20.9% versus 0%) and fear (2.3% versus 0%) significantly improved (p<0.001). Analogical scales scores regarding desire (5 versus 7, p=0.001), arousal (6 versus 8, p<0.001) and satisfaction with sexual life (5 versus 7, p<0.001) also improved. There was a statistically significant improvement (p<0.001) of the POP-Q stages after the surgery. However, there was no statistically significant correlation between changes in vaginal dimensions and changes in sexual function. CONCLUSIONS: after pelvic reconstructive surgery, there was a significant improvement in the quality of sexual life and of the POP-Q stages. However, there was no correlation between them.
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    Caracterizacao da dinamica de colonizacao/infeccao de trato genital por Candida em mulheres gravidas infectadas peli virus HIV
    (Universidade Federal de São Paulo (UNIFESP), 2004) Mazzarolo, Maria Fernanda Gennari [UNIFESP]
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    Comparison of vaginal wall sling and modified vaginal wall sling for stress urinary incontinence
    (Associação Paulista de Medicina - APM, 2000-05-04) Bezerra, Carlos Alberto; Sadi, Marcus Vinicius [UNIFESP]; Faculty of Medicine of the ABC Foundation, São Paulo Urology Division; Universidade Federal de São Paulo (UNIFESP)
    CONTEXT: There are several controversies about which is the best form of surgical treatment for stress urinary incontinence in women. The vaginal wall sling in its original and modified form were presented by Raz as new options for treatment of these conditions, but there is a lack of comparative clinical trials using both techniques. OBJECTIVE: To compare the effectiveness of the original and the modified vaginal wall sling. DESIGN: A comparative, prospective, non-randomized clinical trial. SETTING: Public and private health care units (Urology Division, Faculty of Medicine of the ABC Foundation, and Universidade Federal de São Paulo (UNIFESP) / Escola Paulista de Medicina). PARTICIPANTS: Twenty patients with anatomical and intrinsic sphincter deficiency stress urinary incontinence were surgically treated for evaluating the initial results of the vaginal wall sling, from February 5, 1994, to June 27, 1996. INTERVENTIONS: The patients were divided into two groups. Group A (n = 10) were treated with the original vaginal wall sling. Group B (n = 10) were treated with the modified vaginal wall sling. Both groups were statistically similar according to clinical and urodynamic parameters. MAIN MEASUREMENTS: Cure and complication rates. RESULTS: Follow-up ranged from 19 to 43 months (median = 28) for group A. The overall cure rate was 70%. Fifty per cent of the patients had urinary retention of 7 to 35 days. There were no major complications. Follow-up ranged from 14 to 26 months (median = 18) for Group B. The cure rate was 80%. Two patients had urinary retention of 7 and 55 days. There were no major complications. CONCLUSIONS: The vaginal wall sling is as effective as the modified vaginal wall sling but has a higher rate of urinary retention.
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    Estudo comparativo randomizado entre enxerto de mucosa oral e de túnica vaginal para uretroplastia anterior
    (Universidade Federal de São Paulo (UNIFESP), 2013) Ximenes, Sergio Felix [UNIFESP]; Soler, Roberto [UNIFESP]; http://lattes.cnpq.br/1116555724359776; http://lattes.cnpq.br/3111239274730825
    Estudo comparativo randomizado entre enxerto de mucosa oral e de tunica vaginal para uretroplastia anterior Introdução e Objetivos: O enxerto de mucosa oral e o mais utilizados atualmente para uretroplastia de substituicao. Suas propriedades permitem uma reconstrucao uretral segura e com bons resultados. A possibilidade do uso da tunica vaginal como enxerto alternativo a mucosa oral e bem atraente, pois ele e de facil obtencao, geralmente pela mesma incisao da uretroplastia. Para avaliar se o enxerto de tunica vaginal e uma alternativa comparavel ao enxerto de mucosa oral, propusemos o presente estudo. Material e Metodos: Vinte e quatro pacientes do sexo masculino, com media de idade de 46,5 anos, e quadro de estenose de uretra anterior com indicacao de uretroplastia com uso de enxertos foram igualmente randomizados para receber mucosa oral (grupo 1) ou tunica vaginal (grupo 2). O enxerto foi posicionado ventral ou dorsalmente por decisao tecnica no intra-operatorio. Todos os pacientes foram mantidos com cateter uretral por 4 semanas e seguidos por dois anos com urofluxometria livre nos meses 1, 3, 6, 12, 18 e 24. Pacientes com sintomas de esvaziamento ou com urofluxometria inferior a 15 ml/s, foram submetidos a uretrocistografia retrograda e miccional. Foi definido como falha a necessidade de qualquer instrumentacao uretral, incluindo dilatacoes, uretrotomias ou uretroplastia, durante o seguimento. Resultados: Nao houve diferenca entre os dois grupo quanto a idade, peso, indice de massa corporea, comorbidades, etiologia ou uretroplastia previas. Nao houve diferenca em relacao ao local da estenose, com tres casos de estenose de uretra peniana e nove de uretral bulbar em cada grupo. A extensao da estenose e do enxerto foi respectivamente 3,8 e 4,4 cm no grupo 1 e 4,9 e 5,0 cm no grupo 2. Os enxertos foram posicionados ventralmente em tres pacientes, e dorsalmente em 8, em ambos os grupos. O tempo medio de cirurgia foi de 112,9 minutos no grupo 1 e 150 minutos no grupo 2. Apos 24 meses de seguimento, a taxa de sucesso foi de 75% no grupo de mucosa oral e 50% no grupo tunica vaginal (p=0,4). O tempo medio para recorrencia da estenose foi de 15 meses no grupo 1 e 7,7 meses no grupo 2 (p=0,14). Apos analise preliminar dos dados, foi decidido pela nao inclusao de novos pacientes, pela aparente, mas nao estatisticamente significante, inferioridade da tunica vaginal. Conclusao: O enxerto de mucosa oral apresentou taxa de sucesso numericamente, embora nao estatisticamente, superior ao enxerto de tunica vaginal. O tempo decorrido ate a falha do tratamento foi numericamente, embora nao estatisticamente, menor no grupo da tunica vaginal comparado ao grupo mucosa oral
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    Estudo dos receptores de estrogênio e progesterona e da espessura da mucosa no epitélio vaginal em mulheres menopausadas portadoras de síndrome genito-urinária submetidas à terapia por laser de CO2 fracionado
    (Universidade Federal de São Paulo (UNIFESP), 2020-08-27) Pinho, Stella Catunda [UNIFESP]; Silva, Ivaldo Da [UNIFESP]; Universidade Federal de São Paulo
    Objective: To analyze the effectiveness of Fractional CO2 Laser (Monalisa Touch ®) and compare it with the use of topical estriol in terms of the quantity and distribution of estrogen and progesterone receptors in the vaginal epithelium and the probable relationship of the thickness of this epithelium in patients with postmenopausal genitourinary syndrome. Method: a total of 25 women with menopausal genitourinary syndrome met the inclusion criteria, a biopsy of the vaginal epithelium was performed with analysis of thickness and initial and final immunohistochemistry. Results: Regarding the estrogen receptor, we found that the levels of this receptor were statistically the same between groups, both at the beginning (p = 0.769) and at the end (p = 0.586) and there was no change over time in the group. estrogen (p = 0.207), but in the laser an increase in the level of this receptor was confirmed (p = 0.004). However, changes in estrogen receptor levels showed no association with treatment (p = 0.238). In the analyzes of the progesterone receptor, we noticed that the levels were statistically the same between the groups, both at the beginning (p = 0.124) and at the end (p> 0.999) and that there was no change over time in both groups: estrogen (p = 0.157) and laser (p = 0.564). These changes in the levels of the progesterone receptor showed no association with the type of treatment (p> 0.999). The correlation between vaginal epithelial thickness and levels of estrogen and progesterone receptors was investigated. For this, the Spearman correlation coefficient (S) was estimated, which quantifies the tendency of growth or decrease between quantitative data and it was found that it was not possible to evidence a significant correlation between epithelium thickness and levels of estrogen and progesterone receptors. Conclusion: It was possible to observe through immunohistochemical analysis that there was an increase in the number of estrogen receptors and maintenance of the amount of progesterone receptors in the vaginal epithelium in both groups, with no statistical difference between them. Another important conclusion is that there was no statistically significant relationship between the thickness and density of these receptors. This research has shown that it is possible to obtain good results with laser therapy which, although more expensive and less accessible so far, has results similar to the gold standard and does not bring the drawbacks of the side effects of hormone therapy.
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    Estudo histoquímico de "polissacarídeos" no muco do epitélio vaginal da cobaia (Cavia porcellus) e suas variações no ciclo estral, na prenhez e na lactação
    (Universidade Federal de São Paulo (UNIFESP), 1977) Angulo,Rubens [UNIFESP]; Camano, Luiz [UNIFESP]
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    Estudo histoquímico de polissacarídeos no muco do epitélio vaginal da coelha (Oryctolagus cuniculus) e suas variações durante a prenhez
    (Universidade Federal de São Paulo (UNIFESP), 1981) Amed, Abes Mahmed [UNIFESP]; Camano, Luiz [UNIFESP]
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    Expressão de genes e de proteínas envolvidos na biossíntese da matriz extracelular no tecido vaginal de mulheres com e sem prolapso de órgãos pélvicos
    (Universidade Federal de São Paulo (UNIFESP), 2011-05-25) Bortolini, Maria Augusta Tezelli [UNIFESP]; Sartori, Marair Gracio Ferreira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: Pelvic organ prolapse (POP) results from the failure of pelvic floor support, and connective tissue abnormalities may be involved in the etiology and/or progression of the dysfunction. We aimed to analyze the differential expression of genes and proteins related to the collagen and elastin biogenesis: lysyl oxidases (LOXs), fibulin-5, fibrillin -1 and -2, and procollagen C proteinase (PCP/BMP1) in vaginal tissue of women without and with advanced POP controlled by hormonal status. Materials and Methods: During total hysterectomy, anterior vaginal wall biopsies were obtained from Caucasian premenopausal women (proliferative phase of menstrual cycle) and postmenopausal women with severe POP (POPQ stage III and IV) and asymptomatic controls (POPQ 0). Total mRNA and protein were extracted using Trizol and RIPA buffer, and the genes and proteins of interest were quantified by real-time RT-PCR and Immunoblotting, respectively. The following analysis were performed: (1) expression of LOX family genes and proteins (LOX and LOXL1-4), fibulin-5, fibrillin-1 and -2 in premenopausal women with and without POP; (2) PCP/BMP1 gene and protein expression in vaginal tissue of pre- and postmenopausal POP women, and respective controls. Wilcoxon signed-rank and Fisher tests were used for statistical analysis (p<0.05). Results: Samples from 15 premenopausal patients and 11 controls were obtained for study (1); 39 premenopausal (POP=23 and Control=16) and 18 postmenopausal women samples (POP=13 and Control=5) for study (2). We observed: (1) significant decrease in expression of LOX, LOXL1 and LOXL3 genes, as well as LOX and LOXL3 proteins in vaginal tissue of premenopausal POP patients compared with asymptomatic women (p<0.05); (2) PCP/BMP1 gene downregulation in the vagina of women with severe POP compared with controls, in both premenopausal and postmenopausal phase (both p=0.01); significant reduction of 130 kDa, 92.5 kDa and 82.5 kDa PCP/BMP1 isoforms in vaginal tissue of postmenopausal patients (p=0.01), and 130 kDa isoform upregulation in premenopausal women with severe POP (p=0.009), compared with their respective controls. Conclusion: The expression of LOXs enzymes and PCP/BMP1 are altered in vaginal tissue of women with severe POP, and are modulated by hormonal status. Dysregulation of these enzymes involved in the extracellular matrix biogenesis may contribute to impaired tissue and vaginal support, and may be involved in POP development.
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    Expression of Bone Morphogenetic Protein-1 in vaginal tissue of women with severe pelvic organ prolapse
    (Elsevier B.V., 2011-06-01) Bortolini, Maria Augusta Tezelli [UNIFESP]; Shynlova, Oksana; Drutz, Harold P.; Girão, Manoel João Batista Castello [UNIFESP]; Castro, Rodrigo de Aquino [UNIFESP]; Lye, Stephen; Alarab, May; Univ Toronto; Mt Sinai Hosp; Universidade Federal de São Paulo (UNIFESP)
    OBJECTIVES: To analyze the differential gene and protein expression of Bone Morphogenetic Protein-1 in vaginal tissue of women with advanced pelvic organ prolapse and controls.STUDY DESIGN: We sampled the anterior vaginal wall of 39 premenopausal (23 patients and 16 controls), and 18 postmenopausal women (13 patients and 5 controls) during hysterectomy. Total mRNAs and proteins were quantified by real-time RT-PCR and immunoblotting.RESULTS: Bone Morphogenetic Protein-1 gene expression was decreased in pre- and postmenopausal pelvic organ prolapse patients compared with asymptomatic women (P = .01). the expression of 130 kDa, 92.5 kDa, and 82.5 kDa isoforms of Bone Morphogenetic Protein-1 were down-regulated in postmenopausal patients (P = .01), whereas the 130 kDa isoform expression was up-regulated in premenopausal patients (P = .009), when compared with respective controls.CONCLUSION: the Bone Morphogenetic Protein-1 expression in human vagina was altered in patients with severe pelvic organ prolapse and influenced by menopausal status. Dysregulation of Bone Morphogenetic Protein-1 may contribute for a deficient vaginal connective tissue and support.
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    Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse
    (Wiley-Blackwell, 2012-01-01) Bortolini, Maria Augusta Tezelli [UNIFESP]; Shynlova, Oksana; Drutz, Harold P.; Castro, Rodrigo de Aquino [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Lye, Stephen; Alarab, May; Univ Toronto; Universidade Federal de São Paulo (UNIFESP); Mt Sinai Hosp
    Aims We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM-myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma-actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls. Methods: During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real-time RT-PCR. Results: MHY11 gene expression was down-regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). in the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). the gene expression of CALD1, ACTG2, and TPM1 was significantly down-regulated in vaginal tissue of healthy women after menopause (P < 0.05). Conclusion: Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109-114, 2012. (C) 2011 Wiley Periodicals, Inc.
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    Fibrin adhesive and the vaginal vault synthesis on female rabbits abdominal hysterectomies
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-02-01) Lima, Ari Gonçalves; Taha, Murched Omar [UNIFESP]; Rivoire, Henri Chaplin; Fagundes, Anna Tereza Negrini; Fagundes, Djalma José [UNIFESP]; FURG Department of Surgery; Universidade Federal de São Paulo (UNIFESP); FURG Head of Department of Surgery; Santo Amaro University Medical School
    PURPOSE: To investigate the effectiveness of fibrin glue in comparison with polyglycolic acid suture to promote the closure of rabbit's vaginal vault, after abdominal hysterectomy. METHODS: Twenty female, adults, New Zealand rabbits, were submitted to abdominal hysterectomy and randomly distributed to polyglycolic acid suture (G-PA / n=10) or fibrin glue closure of vaginal vault (G-FG / n=10). Radiograph study allowed identifying vault vaginal suture disrupter or fistulas to urinary bladder or rectum. Videovaginoscopy study allowed identifying the presence of cellulites, abscess formation, tissue granulation or granuloma. Vaginal cuff burst test allowed to identify by the escape of air bubbles and rupture pression record. Histological sections stained with Picrosirius red allowed the measure of fibrous tissue healing. RESULTS: The videovaginoscopy identified a significant difference (Fisher Test p<0.3142) of granulation tissue in the animals of G-PA (40%) in comparison with the G-FG (20%). The gross inspection showed the same relation in the granulation tissue occurrence (Fisher test p< 0.1749) with G-PA (50%) and G-FB (20%). The visceral adhesion to the vaginal vault wound was statistical significant (Fisher test p< 0.1749) with G-PA (50%) and G-FG (20%). The pressure of rupture (mm Hg) of the burst test was similar (p<0.0421) in the animals of G-PA (61.5±19.3) and G-FG (72.5±21.9). The collagen matrix of vault wound healing was similar (p< 0.0231) between the G-PA (31.63±15) and the G-FG (23.2±13.2). CONCLUSION: The vaginal vault closure using the fibrin glue is a safe and reliable procedure after abdominal hysterectomy in female rabbit model.
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    Indirect evaluation of estrogenic activity post heterotopic ovarian autograft in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2008-08-01) Damous, Luciana Lamarão [UNIFESP]; Silva, Sônia Maria da [UNIFESP]; Simões, Ricardo dos Santos [UNIFESP]; Sakano, Célia Regina de Souza Bezerra [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); São Paulo University Gynecological Division
    PURPOSE: To morphologically evaluate the estrogenic effect on the uterus and vagina of rats submitted to ovarian autografts. METHODS: Twenty Wistar EPM-1 adult rats were bilaterally ovariectomized, followed by ovarian transplants in retroperitoneal regions. The animals were divided in four groups of five animals, according to the day of euthanasia: G4, G7, G14 and G21, corresponding to the 4th, 7th, 14th and 21st day after surgery, respectively. Vaginal smears were collected from the first day of surgery until euthanasia day. After that, the vagina and uterus were removed, fixed in 10% formaldehyde and submitted to histological analysis and stained with hematoxiline and eosine. RESULTS: All animals showed estrous cycle changes during the experiment. In 4th day, the uterus showed low action of estrogen with small number of mitosis and eosinophils as well as poor development. On the 7th day, the endometrium was atrophic without mitotic signals and presented a small number of eosinophils. On the 14th and 21th days the histological findings were similar, with the presence of mitosis in the endometrial glands and intense leucocyte infiltration with a large number of eosinophils. Morphometric results showed that the endometrial and myometrial thickness as well as the number of eosinophils presented the highest values during the 14th and 21th days of the evaluation. The 7th day group also presented the lowest eosinophil numbers. Vaginal epithelium features were: 4th and 7th day groups presented non-keratinized stratified epithelium with 5 and 2 cell layers, respectively. The 14th and 21st day groups presented non-keratinized stratified epithelium with 14 and 15 cell layers. CONCLUSION: Experimental ovarian autografts in the evaluated organs presented maximum estrogen activity after the 21st day of surgery, according to morphological and morphometric data.
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    Método de hibridização "in situ" para detecção da infecção pelo papiloma vírus humano (HPV) em regiões de vulva, vagina e colo uterino
    (Universidade Federal de São Paulo (UNIFESP), 2002) Dobo, Cristine [UNIFESP]
    O cancer do colo uterino e o segundo tipo de cancer mais comum entre a populacao feminina, perdendo somente para os carcinomas de mama, e aproximadamente 400.000 novos casos sao diagnosticados a cada ano, em nacoes desenvolvidas e industrializadas. Evidencias epidemiologicas e sobretudo atraves das investigacoes clinicas, sorologicas e com bases nos estudos de biologia molecular, indicam que a infeccao pelo Papiloma virus humano (HPV) corresponde ao principal fator etiologico da carcinogenese cervical, principalmente associado aos outros fatores de risco conhecidos: baixas condicoes socio-economicas, inicio precoce da atividade sexual, pluralidade de parceiros sexuais, uso de contraceptivos orais, habito de fumar. E a doenca sexualmente transmissivel mais comum entre mulheres jovens, atingindo 20 por cento a 46 por cento em varios paises. Para a deteccao da presenca de DNA virai, foram estudadas 332 biopsias retrospectivas ao ano de 1999, de uma amostra de 269 mulheres, com idade media de 30 anos. A distribuicao das amostras mostrou que a maioria das biopsias pertenciam a regiao de colo uterino (49,4 por cento), seguido das biopsias realizadas em regioes da vulva (36,4 por cento) e da vagina (14,2 por cento). O metodo de hibridizacao in situ apresentou reacao positiva na deteccao do HPV em 92 (27,71 por cento) exames: 30 (18,29 por cento) das 164 biopsias da regiao do colo uterino, 42 (34,71 por cento) das 121 biopsias da regiao de vulva e 20 (42,55 por cento) das 47, em vagina. Das 142 amostras cujos resultados . anatomo-patologicos foram de processos inflamatorios com alteracoes morfologicas sugestivas de infeccao pelo HPV,...(au)
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    The soybean concentrated extract proliferates the vagina of adult rats
    (Lippincott Williams & Wilkins, 2011-01-01) Ferraz Carbonel, Adriana Aparecida [UNIFESP]; Pinheiro Baracat, Maria Candida [UNIFESP]; Simoes, Ricardo Santos; Simoes, Manuel Jesus [UNIFESP]; Baracat, Edmund Chada; Soares, Jose Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    Objective: the aim of this study was to evaluate changes induced on the vagina of ovariectomized rats after treatment with soybean concentrated extract or conjugated equine estrogens and the association of both drugs.Methods: We conducted an experimental study with 50 ovariectomized rats that were randomly divided into five equal groups of 10 animals: GI received vehicle, GII received soybean concentrated extract 46 mg/kg per day, GIII received soybean concentrated extract 120 mg/kg per day, GIV received conjugated equine estrogens 50 mu g/kg per day, and GV received conjugated equine estrogens 50 mu g/kg and soybean concentrated extract 46 mg/kg per day. the substances were administered by gavage during 21 consecutive days. After that, the animals were killed under anesthesia and the vagina was removed for histological and immunohistochemical analysis. Data were initially submitted to analysis of variance. Whenever a significant difference was detected, the study was complemented with the Tukey-Kramer test for multiple comparisons.Results: GII did not show any differences on the vaginal epithelium or collagen compared with GI. GIII presented an increase in vaginal epithelium and collagen amount. GIV had the highest amount of collagen and the signals of vaginal proliferation. GV did not show any additional effect compared with GIV.Conclusions: Our data suggest that a high dose of isoflavone-rich soy extract may have positive effects on the vaginal structures of ovariectomized rats, but this action is less than that of estrogen treatment on vaginal thickness. in addition, soy extract may not block the estrogen effect on vaginal tissue.