Navegando por Palavras-chave "Transplantation"

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    Adaptación del modelo español de gestión en trasplante para la mejora en la negativa familiar y mantenimiento del donante potencial
    (Universidade Federal de Santa Catarina, Programa de Pós Graduação em Enfermagem, 2011-01-01) Knihs, Neide da Silva [UNIFESP]; Schirmer, Janine [UNIFESP]; Roza, Bartira de Aguiar [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade para o Desenvolvimento do Alto Vale do Itajaí
    The purpose of this study was to compare data related to professional education and profiles of transplant coordinators in Brazil and Spain in order to evaluate opportunities for improvement, due to family rejection and maintaining the potential donor within the country. A detailed evaluation was carried out which compares the formal education data of the transplant coordinator, the coordinator's profile, and the transplant methodology of each country. In Brazil, there is an average of eight hours in training prior to beginning work with transplants, whereas in Spain, 42 hours are necessary. In Brazil 22.9 % of transplant coordinators are physicians, while in Spain 95% are physicians. The average length of time that the transplant coordinator has worked in Brazil was nine months and twenty-two days, while in Spain it was sixty months. The National Transplant Organization Management Model (El Modelo de Gestión de la Organizacion Nacional) may be adapted in Brazil as a way to improve transplant coordinator education and family rejection and maintenance losses.
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    Análise dos eventos infecciosos ocorridos com receptores de transplante renal com doador falecido com critério expandido
    (Universidade Federal de São Paulo (UNIFESP), 2020-07-30) Sousa, Sirlei Regina De [UNIFESP]; Barbosa, Dulce Aparecida [UNIFESP]; Universidade Federal de São Paulo
    Introduction: despite advances in dialysis methods and kidney transplantation, mortality from chronic kidney disease remains high. There are few epidemiological studies on risk factors for infection / colonization by multi-resistant bacteria in kidney recipients with Deceased Donor with Expanded Criteria. Objectives: to analyze risk factors for infection / colonization by multi-resistant bacteria in kidney transplant recipients with Deceased Donor with Expanded Criteria. Method: a retrospective, epidemiological, cohort study that analyzed the presence of infection in 466 kidney transplant recipients with an expanded deceased donor. A descriptive analysis of the data presented was performed using absolute and percentage frequencies, means, standard deviations, minimum and maximum values, when appropriate, univariate and multivariate analysis for risk factors for infection. The level of significance adopted was 0.05. The statistical program used to perform the calculations was RStudio, version 1.2.1335 and R, version 3.4.4. Results: A total of 466 kidney transplant recipients with Deceased Donor with Expanded Criterion were included in the study. 63% were male, mean age 47 years, mixed race (44.2%) prevailed, 89% were positive for cytomegalovirus, 49% received even a bag of hemacene concentrate before transplantation. The mean cold ischemia time was 1503 minutes, 53.4% underwent urethral anastomosis using the Gregoir technique, requiring hemodialysis after transplantation 3 to 4 times, the use of thymoglobulin was observed in 39.9% for one dose and in 15.9% for 4 doses. The main cause of death of the donor was stroke (31.5%) followed by traumatic brain injury (32%). The presence of acute tubular necrosis was observed in 64.2% of the donor kidney before transplantation. A total of 551 episodes of infection were recorded, 162 by cytomegalovirus, 80 by urinary tract infection and others. Episodes of infections were 70.2% in kidney transplant recipients with Deceased Donor with Expanded Criterion. The risk factors for infection for the 327 (70.20%) recipients were using the immunosuppressive protocol Tacrolimus, Prednisone and Azathioprine, Tacrolimus, Prednisone and Mycophenolate Sodium, induction with thymoglobulin and presence of CMV infection. The incidence of infection with multidrug-resistant bacteria was 6.1%. The most common infection was Urinary Tract Infection (70%), and the carbapenemase-producing etiologic agent Klebsiella pneumoniae was diagnosed in eleven urine cultures (78.50%). Klebsiella pneumoniae carbapenemase-producing pneumonia was present in four patients and in one due to surgical site infection. One patient had multidrug-resistant Eschichia Coli urinary tract infection, a vancomycin-resistant Enterococcus blood-correcting infection, and two had carbapenemase-producing Klebsiella pneumoniae and Eschichia Coli. Survival was 96% among patients with infection. Patients without infection survived at a rate of 100% two years after the transplant. Survival was shorter for recipients who had CMV infection in the first month after transplantation. Conclusions: the most frequent infectious episodes were due to cytomegalovirus and urinary tract infection. The etiologic agent most present in the result of urine culture was the carbapenemase-producing Klebsiella pneumoniae. The risk factors for infection were length of stay, having infection with Cytomegalovirus, use of Tacrolimus, Prednisone and Azathioprine, Tacrolimus, Prednisone and Mycophenolate Sodium, induction with Timoglobulin and diabetes. Death occurred in 3.3% of the studied population and the main cause of death was infectious. Survival was 96% among patients with infection.
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    Assessment of surgical outcomes of limbal transplantation using simple limbal epithelial transplantation technique in patients with total unilateral limbal deficiency
    (Consel Brasil Oftalmologia, 2016) Queiroz, Ana Gabriela [UNIFESP]; Oiticica Barbosa, Martina Maria [UNIFESP]; Santos, Myrna Serapiao [UNIFESP]; Barreiro, Telma Pereira [UNIFESP]; Pereira Gomes, Jose Alvaro [UNIFESP]
    This study aimed to evaluate the effectiveness of the novel simple limbal epithelial transplantation (SLET) technique, which reduces the risk of iatrogenic limbal stem cell deficiency in the donor eye. Four patients with total unilateral limbal stem cell deficiency received a limbal graft, measuring 4 mm x 2 mm, from the contralateral healthy eye in a single surgical procedure. The graft was divided into 10-20 pieces and distributed on the corneal surface. At 6-month follow-up, a completely avascular corneal epithelial surface was obtained in two patients, and there was improvement in visual acuity in one patient. The limbal grafts did not adhere to the cornea in one patient. No serious complications related to the surgery were observed in this study.
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    Autologous grafting of extraocular muscles: experimental study in rabbits
    (Conselho Brasileiro de Oftalmologia, 2005-06-01) Meireles-teixeira, Jorge [UNIFESP]; Bicas, Harley E. A.; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    PURPOSE: To evaluate the feasibility of autologous extraocular muscle grafting as a type of muscle expansion surgery. METHODS: The left superior rectus muscle of twenty-nine rabbits was resected and this fragment was attached to the endpoint of the respective right superior rectus (test group). Thereafter, the superior rectus of the left eye was reattached to the sclera (control group). Both groups were examined during different postoperative periods in order to assess their outcomes. RESULTS: The presence of hyperemia was slightly more frequent in the grafted group. Secretion and muscle atrophy were negligible in both groups. Fibrosis was greater in grafted animals. These muscles were weaker than the control muscles, although the force required to split muscular parts was always greater than the physiological one. CONCLUSIONS: This surgical technique was reliable and useful if one intends to achieve muscle expansion without the intrinsic risks of dealing with heterologous/artificial materials.
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    Autologous stem cell transplantation improves quality of life in economically challenged, Brazilian multiple myeloma patients
    (Faculdade de Medicina / USP, 2011-01-01) Etto, Leina Y [UNIFESP]; Morel, Vânia Maris [UNIFESP]; Silva, Vanderleia C [UNIFESP]; Hungria, Vania Tietsche de Moraes; Ciconelli, Rozana Mesquita [UNIFESP]; Almeida, Manuella S. S [UNIFESP]; Oliveira, José Salvador Rodrigues de [UNIFESP]; Barros, José Carlos; Durie, Brian G; Colleoni, Gisele Wally Braga [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Santa Casa de Misericórdia de São Paulo Faculdade de Ciências Médicas; Cedars-Sinai Outpatient Cancer Center
    OBJECTIVES: 1) To characterize the impact of multiple myeloma on the quality of life of patients treated in two public institutions in São Paulo State, Brazil, using a generic Short Form 36 Health Survey and a questionnaire specific for oncologic patients (QLQ-C30) upon diagnosis, after the clinical treatment, and at day +100 after autologous stem cell transplantation; 2) to evaluate whether autologous stem cell transplantation can improve the quality of life of our economically challenged population aside from providing a clinical benefit and disease control. METHODS: We evaluated 49 patients with multiple myeloma (a total of 70 interviews) using the two questionnaires. The scores upon diagnosis, post-treatment/pre-autologous stem cell transplantation, and at D+100 were compared using ANOVA (a comparison of the three groups), post hoc tests (two-by-two comparisons of the three groups), and paired t-tests (the same case at two different times). RESULTS: Of the included patients, 87.8% had a family budget under US $600 (economic class C, D, or E) per month. The generic Short Form 36 Health Survey questionnaire demonstrated that physical function, role-physical, and bodily pain indices were statistically different across all three groups, favoring the D+100 autologous stem cell transplantation group (ANOVA). The questionnaire specific for oncologic patients, the QLQ-C30 questionnaire, confirmed what had been demonstrated by the Short Form 36 Health Survey with respect to physical function and bodily pain, with improvements in role functioning, fatigue, and lack of appetite and constipation, favoring the D+100 autologous stem cell transplant group (ANOVA). The post hoc tests and paired t-tests confirmed a better outcome after autologous stem cell transplantation CONCLUSION: The questionnaire specific for cancer patients seems to be more informative than the generic Short Form 36 Health Survey questionnaire and reflects the real benefit of autologous stem cell transplantation in the quality of life of multiple myeloma patients in two public Brazilian institutions that provide assistance for economically challenged patients.
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    Caracterização in vitro de precursores neuronais da eminência gangliônica medial e avaliação in vivo do seu potencial anticonvulsivo após transplante em animais epilépticos
    (Universidade Federal de São Paulo (UNIFESP), 2016-05-13) Romariz, Simone Amaro Alves [UNIFESP]; Longo, Beatriz Monteiro [UNIFESP]; http://lattes.cnpq.br/0245964878412260; http://lattes.cnpq.br/9325741054357205; Universidade Federal de São Paulo (UNIFESP)
    Epilepsia, crises epilépticas espontâneas e recorrentes se desenvolvem a partir de disparos neuronais contínuos e de alta frequência que podem ser gerados por uma disfunção sináptica inibitória. Portanto, as consequências da perda ou disfunção de interneurônios GABAérgicos no desenvolvimento do quadro epiléptico são importantes. Os interneurônios inibitórios se originam na região do telencéfalo conhecida como eminência gangliônica medial (EGM). Células progenitoras da EGM têm a capacidade de migrar e se diferenciar em interneurônios inibitórios GABAérgicos, modificando o tônus inibitório quando transplantadas no cérebro do hospedeiro. Assim, o transplante de células progenitoras derivadas da EGM pode modificar a circuitaria neuronal em disfunções neurológicas onde há alteração da função inibitória, como na epilepsia. No entanto, o transplante de células da EGM requer uma grande quantidade de células fetais, esbarrando em questões éticas. A expansão in vitro desses progenitores em neuroesferas pode ser uma alternativa para a aplicação terapêutica das células da EGM em maior escala. Nosso objetivo foi comparar diferentes condições de cultivo de neuroesferas derivadas da EGM quanto ao padrão de diferenciação neuronal e expressão de genes presentes na EGM in vitro e, avaliar in vivo após transplante em animais adultos no período epileptogênico, o potencial anticonvulsivo e de diferenciação em interneurônios inibitórios de células oriundas da EGM cultivadas ou não como neuroesferas. Os resultados in vitro mostraram que a remoção dos fatores de crescimento EGF e FGF2, bem como a adição de ácido retinóico ao meio de cultivo de neuroesferas oriundas da EGM aumentam a proporção de neurônios e a expressão de genes relacionados à especificação celular de interneurônios inibitórios nas neuroesferas, comparado à condição padrão de cultura. Os resultados in vivo indicam que as células derivadas da EGM que não foram cultivadas como neuroesferas originam mais interneurônios inibitórios e apresentam um efeito anticonvulsivo mais eficaz quando comparadas com células da EGM cultivadas como neuroesferas, independente das modificações realizadas no meio de cultivo. Dentre as células da EGM cultivadas como neuroesferas, as que foram cultivadas na presença de fatores de crescimento reduziram a frequência de crises do tipo IV e se diferenciaram, preferencialmente, em astrócitos. Concluímos que os precursores neuronais oriundos da região da EGM apresentam um potencial anticonvulsivo e que a técnica de expansão dessas células em neuroesferas, mesmo com as modificações no meio de cultivo, não favoreceu o aumento de interneurônios inibitórios após transplante em animais epilépticos. Apesar disso, as células cultivadas como neuroesferas na condição padrão se diferenciaram em astrócitos após o transplante, um tipo celular que pode ser importante no controle das crises espontâneas e recorrentes devido a possiveis mecanismos de redução de crises que devem ser melhor investigados.
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    Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
    (Consel brasil oftalmologia, 2016) Vianna, Lucas Monferrari Monteiro [UNIFESP; Li, Hao-Dong; Holiman, Jeffrey D.; Stoeger, Christopher; Belfort, Rubens Junior [UNIFESP]; Jun, Albert S.
    Purpose: To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Methods: Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM
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    Comparação dos meios de preparação e preservação de membrana amniótica humana para uso no tratamento de doenças da superfície ocular
    (Universidade Federal de São Paulo (UNIFESP), 2015-12-31) Ferenczy, Peter Alexander Von Harbach [UNIFESP]; Sousa, Luciene Barbosa de [UNIFESP]; http://lattes.cnpq.br/2367491641205774; http://lattes.cnpq.br/4522482036126391; Universidade Federal de São Paulo (UNIFESP)
    Atualmente a membra amniótica (MA) tem obtido importância devido à comprovada capacidade de reduzir inflamação, auxiliar a cicatrização e epitelização, possuindo propriedades antimicrobianas e antivirais, além de baixa imunogenicidade. As indicações de seu uso na oftalmologia têm aumentado muito nas duas últimas décadas. Objetivo: descrever a estrutura básica e as propriedades biológicas da MA em relação aos componentes da sua matriz extracelular e fatores de crescimento, as consequências de diferentes técnicas empregadas na sua preservação e esterilização, métodos para remoção do epitélio e a comparação dos custos dos diferentes meios de conservação atualmente empregados. Desenho do estudo: revisão bibliográfica. Método: Pesquisa nas bases de dados Bireme, Pubmed, Cochrane, Scielo e Lilacs com as palavras-chave: membrana amniótica, transplante, reconstrução da córnea, doenças da conjuntiva. Resultados: A literatura é vasta na descrição dos efeitos de diversos agentes e técnicas na preparação da MA, dentre elas sua preservação, esterilização e desepitelização. A membrana desnuda tem sido a escolha para a reconstrução da superfície ocular, pois facilita a cicatrização. Em relação aos agentes conservantes, o glicerol é o meio mais utilizado mundialmente pelo baixo custo e facilidade de manuseio. Conclusão: a comparação das diversas técnicas nos guia na elaboração de protocolos de preparo da MA para uso oftalmológico. A membrana desnuda facilita a cicatrização em relação a com células epiteliais. O glicerol é o meio de conservação mais utilizado pelo baixo custo e facilidade de manuseio.
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    Compreensão dos enfermeiros em setores críticos adulto do Hospital São Paulo quanto à morte encefálica e transplante
    (Universidade Federal de São Paulo (UNIFESP), 2021) Quaglio, Willian Henrique [UNIFESP]; Ferreira, Beatriz Jansen [UNIFESP]; Universidade Federal de São Paulo
    Currently, transplantation of solid organs and human tissues is one of the treatment options to improve the quality of life of people of all ages, who have irreversible chronic disease and in its final stage, that is, the brain death protocol and management it becomes an area of great performance for nurses and their team. The nursing team has an extremely relevant role in direct action to improve the quality of life of transplant patients, however the literature points out that nursing students do not feel prepared to act as nurses in this area due to insufficient training in relation to this topic. Thus, this research aims to identify the understanding of nurses in the Adult Emergency Room and Intensive Care Units of Hospital São Paulo about brain death and transplantation. The research was developed through a quantitative methodology inserted in a descriptive and exploratory study. Data collection took place through the construction of a Likert Scale consisting of 04 dimensions covering 23 assertions that sought to answer the research objectives. This scale was subjected to a content validation using the semantic and cultural equivalences, then it was randomized with points assigned in two scales: 1/2/3/4 and 4/3/2/1 generating three intervals interpretative: from 1 point to 1.99, where a bad perception is identified, demanding short-term corrections; from 2 points to 2.99 the perception is reasonable, requiring corrections in the medium term and from 3 points to 4 points, the perception is good indicating assertiveness regarding the researched object. After applying the scale, it was subjected to the statistical validation process with respect to dispersion and reliability. The study population of 70 people was composed of nurses who work in the Adult Emergency Room and in the Intensive Care Units of Hospital São Paulo. As an inclusion criterion, nurses who had more than six months' experience in these sectors were selected and nurses who did not witness or participated in at least one brain death protocol were excluded. Participants received the Free and Informed Consent Term as well as the acceptance to complete the referred scale. It is expected to highlight the set of actions and challenges of the nursing staff during the process of identifying brain death (BD) as the process of developing the interview with family members of the potential donor and assessing the difficulties and management developed by these professionals in order to qualify and enhance nursing care in these important sectors. The data found were positive about the nurses' understanding of care with the potential donor, enriching the discussions, as they favor the increase in the rate of transplants in Brazil, due to the good conservation of the organ for surgical practice. The understanding of nurses working in the critical adult sectors of Hospital São Paulo, such as ICUs and Emergency Care, about brain death and transplantation was reasonable, considering that no dimension had a rating below 1.99, requiring no corrections to be made short term.
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    Conjunctival epithelial cells cultivated ex vivo from patients with total limbal stem cell deficiency
    (Wichtig Editore, 2015-01-01) Silber, Paulo Caldas [UNIFESP]; Ricardo, Jose Reinaldo da Silva [UNIFESP]; Cristovam, Priscila Cardoso [UNIFESP]; Hazarbassanov, Rossen Mihaylov [UNIFESP]; Dreyfuss, Juliana Luporini [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose: Reconstruction of the ocular surface is challenging. As an alternative to mucosal and limbal epithelial, we study the feasibility of cultivated human conjunctival epithelial (HCjE) cells of patients with total limbal stem cell deficiency (LSCD).Methods: We studied superior forniceal conjunctival biopsies harvested from 9 living donors with total LSCD of several etiologies who underwent surgery for ocular surface reconstruction. the conjunctival explants were cultivated on serum and growth factor supplemented DMEM/ F12 under submerged conditions on denuded human amniotic membrane and tissue culture dishes. the area of cell growth was assessed. Cell morphology was analyzed by light microscopy, impression cytology, and transmission electron microscopy. Cultures were evaluated for epithelial cytokeratins (CK3, CK19), proliferation marker (Ki-67), and putative stem cells markers (ABCG2 and p63). Confocal immunofluorescence was also performed to assess CK3, CK19, Ki-67, ABCG2, and p63.Results: the HCjE cells cultivated ex vivo were successfully expanded on denuded amniotic membrane but with a slower growth than in the tissue culture dish. Transmission electron microscopy showed stratified epithelium with microvilli, desmosomes, and hemidesmosomes. Impression cytology showed PAS+ cells that resembled goblet cells. Immunocytochemical analysis showed positivity for CK3, CK19, Ki-67, ABCG2, and p63. Confocal immunofluorescence was positive for CK3, CK19, Ki-67, ABCG2, and p63.Conclusions: Our results showed that it is possible to cultivate HCjE cells ex vivo of patients with ocular surface diseases. This method is important for ocular surface reconstruction in patients with bilateral total LSCD.
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    Cross-cultural translation of quality instruments in the organ donation process
    (Escola Paulista de Enfermagem, Universidade Federal de São Paulo (UNIFESP), 2014-01-01) Knihs, Neide Da Silva; Schirmer, Janine [UNIFESP]; Roza, Bartira de Aguiar [UNIFESP]; Hospital Santa Isabel; Universidade Federal de São Paulo (UNIFESP)
    OBJECTIVE:To carry out the cross-cultural translation of quality instruments in the organ donation process from the National Transplantation Organization of Spain.METHODS:Methodological research with quantitative approach conducted in three Neurology reference hospitals in the South of the country. The instruments validation was carried out by mean of the steps of translation, backtranslation, synthesis and expert committee. The reliability was conducted by means of inter-observer equivalence and test and retest stability.RESULTS:There was a 100% match among all the judges in 89% of the items in the cross-cultural translation (11% of the items were not equivalent). In the reliability analysis, the Kappa coefficients corresponded to 0.86 and 0.95.CONCLUSION:The versions of the instruments were considered valid and reliable for use by institutions that carry out the organ donation process in Brazil.
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    Cytokine Dosage in Fresh and Preserved Human Amniotic Membrane
    (Lippincott Williams & Wilkins, 2016) Bomfim Pereira, Mario Genilhu [UNIFESP]; Gomes, José Álvaro Pereira [UNIFESP]; Rizzo, Luiz Vicente; Cristovam, Priscila Cardoso [UNIFESP]; Silveira, Laila Christina; Universidade Federal de São Paulo (UNIFESP)
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    Cytokine Dosage in Fresh and Preserved Human Amniotic Membrane
    (Lippincott Williams & Wilkins, 2016) Bomfim Pereira, Mario Genilhu [UNIFESP]; Pereira Gomes, Jose Alvaro [UNIFESP]; Rizzo, Luiz Vicente; Cristovam, Priscila Cardoso [UNIFESP]; Silveira, Laila Christina
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    Da utilização de celulose biossintética na cicatrização de perfuração traumática de membrana do tímpano em chinchila laniger
    (Universidade Federal de São Paulo (UNIFESP), 2001) Diniz Junior, Jose [UNIFESP]; Munhoz, Mário Sérgio Lei [UNIFESP]
    Dos resultados obtidos a comparacao da utilizacao de celulose biossintetica com a evolucao natural da cicatrizacao de perfuracao traumatica provocada em membranas do timpano de chinchilla laniger, sob o ponto de vista macroscopico, a rapidez do fechamento e, microscopico, a ocorrencia de reacoes teciduais, podemos concluir que: 1. O fechamento da perfuracao traumatica provocada ocorreu mais precocemente no grupo que utilizou a celulose biossintetica. 2. Ocorreram reacoes teciduais de espessamente e inflamacao no grupo que utilizou a celulose biossintetica
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    Description and evaluation of experimental models for uterine transplantation in pigs
    (Inst Israelita Ensino & Pesquisa Albert Einstein, 2017) de Oliveira, Emerson; da Silva Tavares, Kelly Alessandra; Vieira Gomes, Mariano Tamura; Salzedas-Netto, Alcides Augusto [UNIFESP]; Ferreira Sartori, Marair Gracio [UNIFESP]; Castro, Rodrigo Aquino [UNIFESP]; Fernandes, Cesar Eduardo; Batista Castello Girao, Manoel Joao [UNIFESP]
    Objective: To evaluate the technique of uterine transplantation and the use of drugs used in the process of immunosuppression. Methods: We included 12 sows, and immunosuppression was performed with minimal doses of cyclosporine, and cross-match was done to exclude the possibility of blood incompatibility. Hysterectomy was performed in the donor under general anesthesia, with the removal of the aorta and inferior vena cava in monobloc, and anastomosis of these vessels was made in the recipient. Results: Six experiments were performed, and on the immediate postoperative period, five animals had good reperfusion. However, on the seventh postoperative day, histological analysis showed rejection in five animals. Conclusion: The experimental model of uterine transplantation is feasible, but monitoring doses of immunosuppressants is pivotal to prevent rejection episodes.
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    Efeito do enxerto autólogo de pericôndrio costal com butil-2-cianoacrilato em lesão provocada na cartilagem articular do joelho de coelhos
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 1999-10-01) Xavier, Mário Sérgio Viana; Souza, Virgínio Cândido Tosta de; Gomes, Paulo de Oliveira [UNIFESP]; Corrêa, José Carlos; Novo, Neil Ferreira [UNIFESP]; Juliano, Yara [UNIFESP]; Faculdade de Ciências Médicas de Pouso Alegre; Universidade Federal de São Paulo (UNIFESP)
    The purpose of this study was to verify the effect of the perichondrium graft with butyl-2-cyanoacrylate in provoked injury in the articular cartilage of rabbit`s knee. Male adult animals were used, divided in 2 groups, called Group A and Group B, with 17 animals each. The Group A animals were reoperated in 4 weeks and the Group B animals in 8 weeks. A 2 cm fragment was taken out from the 7th costal cartilage from which the perichondrium was removed. Two osteo-cartilaginous cylinders were taken out from each medialis condyles of the femurs in the same animal. The articular cartilage of the cylinder was replaced in one side by the perichondrium with a thin layer of sticking-tissue in its external face and only the articular cartilage was removed from the other side. The cylinders were replaced in the femurs. Macroscopically, in Group A, most of the injuries which received the perichondrium were found completely covered with tissue and all the lesions without perichondrium were only partilly covered. In Group B, no macroscopic significant difference in the covering of the injuries was found. Statistically, There was no microscopical significant difference between the injuries with and without perichondrium of the Group A and Group B and neither groups A and B.
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    Effect of neuronal precursor cells derived from medial ganglionic eminence in an acute epileptic seizure model
    (Wiley-Blackwell, 2010-01-01) Calcagnotto, Maria E. [UNIFESP]; Ruiz, Lorena P. [UNIFESP]; Blanco, Miriam M. [UNIFESP]; Santos-Junior, Jair G. [UNIFESP]; Valente, Maria F. [UNIFESP]; Patti, Camila [UNIFESP]; Frussa-Filho, Roberto [UNIFESP]; Santiago, Marcelo F.; Zipancic, Ivan; Alvarez-Dolado, Manuel; Mello, Luiz E. [UNIFESP]; Longo, Beatriz M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Ctr Andaluz Biol Mol & Med Regenerat CABIMER; Fac Ciencias Med Santa Casa; Universidade Federal do Rio de Janeiro (UFRJ)
    P>Most of the gamma-aminobutyric acid (GABA)ergic interneurons in the cerebral cortex originate from restricted regions of the ventral telencephalon known as the caudal and medial ganglionic eminence (MGE) and from the preoptic area. It is well established that dysfunction of GABAergic interneurons can lead to epilepsy. During the last decade new approaches to prevent, reduce, or reverse the epileptic condition have been studied, including cell-based therapy from different sources. Recent studies have shown that transplanted neuronal precursor cells derived from MGE have the ability to migrate, differentiate into inhibitory GABAergic interneurons, and integrate into cortical and hippocampal networks, modifying the inhibitory tone in the host brain. Therefore, transplantation of neuronal precursors derived from MGE into the postnatal central nervous system (CNS) could modify the neuronal circuitry in neurologic diseases in which inhibitory synaptic function is altered, such as in epilepsy. Here, we evaluated the seizure susceptibility of mice transplanted with MGE-derived cells in the maximum electroconvulsive shock (MES) model and we review some data from different studies using GABAergic precursor or GABA-releasing cell grafts in animal models of seizure and epilepsy.
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    Estudo do reparo do ferimento de colon com o lado seroso da parede de jejuno, utilizando cianoacrilato e cola de fibrina
    (Colégio Brasileiro de Cirurgiões, 2006-04-01) Fontes, Carlos Edmundo Rodrigues; Taha, Murched Omar [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Prado Filho, Orlando; Ferreira, Marcos Victor; Mardegan, Marino José; Universidade Estadual de Maringá Clínica Cirúrgica; Universidade Federal de São Paulo (UNIFESP)
    BACKGROUND: The present experiment aimed to compare the effect of the use of cyanoacrylate and fibrine glue as adesive on repared colon's wounds with the serosal side of jejuno's wall. METHODS: Forty five male, young adult Wister rats, weighting about 260 g , from the University of Maringá were used.After having been deeply anaesthetized (thiopental was used), the animals underwent a surgery, and a pattern 0,5 cm wound was made, 2 cm away from the cecum, in the caudal location. Group one the wound was repaired by using the jejunal serosal wall and suture.On group two the jejunal serosal wall and fibrine glue was used. Finally on group three the repair was made by using the jejunal serosal wall and cyanoacrylate. The animals were submited to roentgenogram with barium enema on the fourth postoperative day to search for stenosis. On the seventh day, after having been anaesthetized, animals were submited to a laparotomy, segments were collected for both macroscopic and microscopic study. RESULTS: Fibrine glue has shown better result on repair, due to the production of collagen fibres. CONCLUSION: The experiment has come to prove that the wound made on rat's colon can be repaired by the jejunal serosal wall joined to the wound either using the fibrine glue or cyanoacrylate.
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    Grafting of GABAergic precursors rescues deficits in hippocampal inhibition
    (Wiley-Blackwell, 2010-01-01) Calcagnotto, Maria E.; Zipancic, Ivan; Piquer-Gil, Marina; Mello, Luiz E. [UNIFESP]; Alvarez-Dolado, Manuel; CABIMER; CIPF; Universidade Federal de São Paulo (UNIFESP)
    P>gamma-Aminobutyric acid (GABA) has an important role in the mechanism of epilepsy. Cell grafts from different sources have been performed to modulate local circuits or increase GABAergic inhibition in animal models of epilepsy. Among the different transplanted cell types, the medial ganglionic eminence (MGE)-derived cells present the best properties to be used in cell-based therapy. in this work we review previous experiences with these cells. in addition, we present new evidence showing their ability to modulate the levels of inhibition in the host brain of mice with alterations in the GABAergic system, caused by the specific ablation of hippocampal interneurons. Grafted GFP+ MGE-derived cells occupied the area of ablation and differentiated into mature NK-1-, SOM-, PV-, CR-, and NPY-expressing interneurons. Inhibitory postsynaptic current (IPSC) frequency and amplitude on CA1 pyramidal cells of the ablated hippocampus significantly increased after transplantation, reaching levels similar to controls. Our data strongly suggest the suitability of MGE-derived cells for the treatment of neurologic conditions for which an increase or modulation of synaptic inhibition is required.
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    Guidelines for the management and treatment of periodic fever syndromes familial mediterranean fever
    (Elsevier science inc, 2016) Terreri, Maria Teresa R. A. [UNIFESP]; Bernardo, Wanderley Marques; Len, Claudio Arnaldo [UNIFESP]; Almeida da Silva, Clovis Artur; Ribeiro de Magalhaes, Cristina Medeiros; Sacchetti, Silvana B.; Leme Ferriani, Virginia Paes; Petry Piotto, Daniela Gerent [UNIFESP]; Cavalcanti, Andre de Souza; de Moraes, Ana Julia Pantoja; Sztajnbok, Flavio Roberto; Feitosa de Oliveira, Sheila Knupp; Arruda Campos, Lucia Maria; Bandeira, Marcia; Sena Teixeira Santos, Flavia Patricia; Magalhaes, Claudia Saad
    Objective: To establish guidelines based on scientific evidence for the management of familial Mediterranean fever. Description of the evidence collection method: The Guideline was prepared from 5 clinical questions that were structured through PICO (Patient, Intervention or indicator, Comparison and Outcome), to search in key primary scientific information databases. After defining the potential studies to support the recommendations, these were graduated considering their strength of evidence and grade of recommendation. Results: 10,341 articles were retrieved and evaluated by title and abstract