Navegando por Palavras-chave "TMA"

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    The extrinsic apoptotic signaling pathway in gastric adenocarcinomas assessed by tissue microarray
    (Elsevier B.V., 2011-01-01) Gomes, Thiago Simao [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Segreto, Helena Regina Comodo [UNIFESP]; Barrezueta, Luis Fernando Mesias [UNIFESP]; Costa, Henrique de Oliveira [UNIFESP]; Lima, Flavio de Oliveira [UNIFESP]; Forones, Nora Manoukian [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The purpose of this investigation was to analyze the immunoexpression of FasL, Fas, FADD, cleaved caspase 8, and cleaved caspase 3 in gastric cancer. Formalin-fixed and paraffin-embedded gastric adenocarcinoma tissues from 87 patients, including adjacent normal tissues, were included on tissue microarray by immunohistochemistry. the tumor and the adjacent normal tissues were positive for FasL in 66.7% and 90.6%, for Fas in 52.8% and 52.4%, for FADD in 67.4% and 82.3%, for cleaved caspase 8 in 27.9% and 37.7%, and for cleaved caspase 3 in 33.7% and 8.3%, respectively. FasL and the FADD from tumor were statistically different in relation to the histological type. Cleaved caspase 8 was statistically different in relation to clinical stage (p = 0.031). the FADD from normal tissue was statistically different in relation to age (p = 0.039), sex (p = 0.055), clinical stage (p = 0.019), and Fas was different in relation to tumor size (p = 0.012). in the tumor, we observed a correlation between FasL and Fas, FasL and FADD, and FasL and cleaved caspase 3. in the adjacent normal tissue, a correlation was observed between FasL and Fas, FasL and FADD. There was no association of another marker with sex, age, clinical stage, and survival. Our results suggest that these proteins mediate the early extrinsic apoptotic pathway in gastric cancer and adjacent normal mucosa. FasL protein binds to Fas protein and subsequently binds to death receptor FADD signaling activation of the extrinsic apoptotic pathway. in this phase, there was inhibition of caspase 8 and, consequently, decreased apoptosis. (C) 2011 Elsevier GmbH. All rights reserved.
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    Transcription-mediated amplification (TMA) for the assessment of viremia in hemodialysis patients with hepatitis C
    (Wiley-Blackwell, 2012-04-01) Bastos, Dauana O. [UNIFESP]; Perez, Renata M.; Souza e Silva, Ivonete Sandra de [UNIFESP]; Lemos, Lara Barros [UNIFESP]; Simonetti, Jose Pascoal; Medina-Pestana, Jose Osmar [UNIFESP]; Silva, Antonio Eduardo B. [UNIFESP]; Ferraz, Maria Lucia [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal do Rio de Janeiro (UFRJ)
    The diagnosis of hepatitis C virus (HCV) infection in hemodialysis patients is difficult particularly due to the presence of intermittent viremia. the aims of this study were: (a) to determine the prevalence of intermittent viremia in hemodialysis patients with anti-HCV antibodies who tested negative for HCV RNA by PCR at the first evaluation and (b) to evaluate the contribution of the transcription-mediated amplification method (TMA) to the diagnosis of viremia in the PCR-negative samples. One hundred and six patients with anti-HCV antibodies and an initial negative result for HCV RNA by PCR were included. An additional sample was collected for a second HCV RNA test by PCR after a minimum interval of 3 months and a positive result characterized intermittent viremia. HCV RNA was investigated by TMA in the PCR-negative sample of patients with intermittent viremia, and in the most recent sample from patients with PCR-negative results in both determinations. Intermittent viremia was observed in 60/106 (57%) patients (57% men; age: 45 +/- 10 years). Fifty-one of the 60 negative samples from patients with intermittent viremia and 29/46 double-negative patients were tested by TMA. This assay detected viremia in 20/51 (39%) samples of intermittent viremia and in 2/29 (7%) of double-negative samples. the results showed that intermittent viremia is frequent in hemodialysis patients who tested negative for HCV RNA by PCR. Therefore, a second HCV RNA test is necessary for all HCV RNA-negative patients. the TMA assay appears to be the best first screening test for viremia in this population. J. Med. Virol. 84:596600, 2012. (C) 2011 Wiley Periodicals, Inc.