Navegando por Palavras-chave "Surgical Flaps"

Agora exibindo 1 - 19 de 19
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Ação do dimetil-sulfóxido na isquemia de retalhos randômicos de pele em ratos
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2004-12-01) Almeida, Kleder Gomes de [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Manna, Mônica Cecília Bochetti [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose: To evaluate through the morphologic study (macro and microscopic) the effectiveness of DMSO in the genesis of the distal necrosis of random skin flaps in rats. Methods: 30 male rats were used, lineage Wistar, weight between 220 and 363g and 3 months medium age .The skin flap (8x2cm) with remained cranial vessels it was unstuck, restored at his bed and sutured with polyamide 4.0. The control-group CT (n=10) don t received any medication, the simulated group -SM (n=10) it received the volume of 1mL of subcutaneous saline solution, divided in ten applications along the skin flap, the experiment group (n=10) it received the injection of 1ml of DMSO5%. After seven days they were appraised the areas of distal necrosis and picked material for the histology study. Results: The measures of the necrosis areas (CT=47.99, SM=58.78, EX=41.57) and the percentages of the necrosis areas (CT=29.98, SM=36.73, EX =23.99) they were shown smaller in the EX group (p < 0,05). The qualitative histology study showed, in the group EX, larger angiogenic presence, smaller destruction of the enclosures and of the conjunctive stroma and presence of fibroblasts in more precocious period than in the two other groups. Conclusion: The analysis of the collected data can infer that DMSO had beneficial action on the random skin flaps, expressed by the smallest area of distal necrosis and for the histology aspect of repairing tissue more precocious.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Acetylcysteine in random skin flap in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2005-04-01) Abla, Luiz Eduardo Felipe [UNIFESP]; Gomes, Heitor Carvalho [UNIFESP]; Percario, Sandro [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: Analyze the ability of Acetylcysteine to reduce distal necrosis in a random skin flap, in the rat. METHODS: The present study utilized 28 adult male Wistar-EPM rats distributed, at random, in two groups of 14 animals. Control group rats (CG) received distilled water and Acetylcysteine group animals (NACG) received NAC (300 mg/kg) by oral infusion, 15 minutes before flap elevation. On the seventh postoperative day, percentage of distal necrosis was determined and skin samples collected in order to allow determination of MDA levels. RESULTS: The mean necrotic area in CG group (control) was 66 % and in NACG group (Acetylcysteine) 52 %, a statistically significant difference according to the Mann-Whitney test (U calc = 25; U crit = 45). MDA levels were lower in the CG flap skin samples than in the NACG samples (U calc = 24; U crit = 45), the oposite being true in the normal skin samples (U calc = 10; U crit = 45). CONCLUSION: Acetylcysteine was effective, according to the model used, reducing the percentage of distal necrosis in NACG rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Suartz, Caio Vinicius; Gaiba, Silvana; França, Jerônimo Pereira de; Aloise, Antonio Carlos [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Santa Cruz Department of Surgery
    PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Suartz, Caio Vinicius [UNIFESP]; Gaiba, Silvana; França, Jerônimo Pereira de; Aloise, Antonio Carlos [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de Santa Cruz Department of Biological Sciences
    PURPOSE:To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS:Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSION: The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Administração tópica de cloridrato de hidralazina na viabilidade de retalho cutâneo randômico em ratos
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2005-04-01) Esteves Junior, Ivaldo [UNIFESP]; Masson, Igor Bordello; Ferreira, Lydia Masako [UNIFESP]; Liebano, Richard Eloin [UNIFESP]; Baldan, Cristiano; Gomes, Alexandre Cavallieri; Universidade Federal de São Paulo (UNIFESP); UNIP; Santa Casa de São Paulo Irmandade de Misericórdia; Universidade Federal de São Carlos
    PURPOSE: Assess the effect of hydralazine hydrochloride, for iontophoresis, on the viability of random skin flaps in rats. METHODS: Sixty Wistar rats was randonly destributed in 4 groups (n=15), these animals was submited as randon dorsal skin flaps as cranial base with measure 10X4 cm. The animals from group 1 was utilized as control, in group 2 was submitted to direct current o 4mA-20' immediately after the surgery and on the two subsequent days. In group 3 the stimulation eletric simulation with hydralazine hydrochloride. In group 4 iontophorese with hydralazine hydrochloride 4mA-20'. The analysis of the results was made on the seventh day post operative and interpreted with test non parametric of Kruskal-Wallis. RESULTS: and the necrotic area stayed fixed in: group 1= 45%; group 2= 39%; group 3= 46% and group 4= 41%, being the statistical analysis did not evedenced any significant. CONCLUSION: The hydralazine hydrochloride when taken for iontophorese was not efficacious in reduce the necrotic area.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Capsaicin on the viability of random-pattern skin flaps in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2010-10-01) Godoy, Gustavo Roberto de [UNIFESP]; Liebano, Richard Eloin [UNIFESP]; Corrêa, Juliana Barbosa [UNIFESP]; Hochman, Bernardo [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To evaluate the effects of capsaicin on the viability of ischemic random-pattern skin flaps in rats. METHODS:Forty EPM1-Wistar rats were randomized into two groups of 20 animals each, the capsaicin group and the control group. A random-pattern skin flap measuring 10 x 4cm was raised and a plastic barrier was placed between the flap and the donor site. After the surgical procedure, the control group was treated with an inert vehicle in the form of a cream applied uniformly to a rayon bandage which, in turn, was applied to the surface of the skin flap. The capsaicin group was treated in the same way, but in this case capsaicin was added to the cream. This procedure was repeated for two consecutive days. RESULTS: There was a significantly smaller amount of flap necrosis in the capsaicin group (35.07%) than in the control group (44.75%) (p=0.035). CONCLUSION:Topical administration of capsaicin improved the viability of ischemic random-pattern skin flaps in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Effect of high frequency transcutaneous electrical nerve stimulation on viability of random skin flap in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2006-06-01) Liebano, Richard Eloin [UNIFESP]; Abla, Luiz Eduardo Felipe [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To determine the effect of high frequency Transcutaneous Electrical Nerve Stimulation (TENS) on viability of random skin flap in rats. METHODS: The sample of this study was 75 Wistar rats. The skin flap measured 10 x 4 cm and a plastic barrier was interposed between the flap and donor site. After the operative procedure, animals of all groups were maintained anesthetized one more hour with electrodes positioned in the base of the flap and submitted to treatment according of their respective group. This procedure was repeated on the two subsequent days. G1: sham stimulation (control), G2: TENS (f = 80 Hz and I = 5 mA), G3: TENS (f = 80 Hz and I = 10 mA), G4: TENS (f = 80 Hz and I = 15 mA), G5: TENS (f = 80 Hz and I = 20 mA). RESULTS: The average percentage of necrotic area was 43,11, 34,65, 49,44, 23,52, 45,10 in groups 1, 2, 3, 4 and 5 respectively. CONCLUSION: The amplitude of 15 mA presented a lower necrotic area than control group and Transcutaneous Electrical Nerve Stimulation was efficient in increasing the random skin flap viability.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12-01) Estevão, Lígia Reis Moura; Medeiros, Juliana Pinto de; Baratella-Evêncio, Liriane; Simões, Ricardo Santos [UNIFESP]; Mendonça, Fábio de Souza; Evêncio-Neto, Joaquim; Federal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal Bioscience; UFRPE Department of Morphology and Physiology Histology Division; Federal University of Pernambuco Department of Morphology Histology Division; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Experimental model for low level laser therapy on ischemic random skin flap in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2006-08-01) Prado, Rodrigo Paschoal [UNIFESP]; Liebano, Richard Eloin [UNIFESP]; Hochman, Bernardo [UNIFESP]; Pinfildi, Carlos Eduardo [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To develop an experimental model to be used in the study of low level Laser therapy on viability of random skin flap in rats. METHODS: The sample was 24 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic sheet was interposed between the flap and donor site. Group 1 (control) underwent sham irradiation with diode laser (830 nm). Group 2 was submitted to laser irradiation with diode laser (830 nm). The animals were submitted to Laser therapy with 36 J/cm² energy density (72 seconds) immediately after the surgery and on the four subsequent days. The probe was usually held in contact with the skin flap surface on a point at 2.5 cm cranial from the flap base. On the seventh postoperative day, the percentage of necrotic area was measured and calculated. RESULTS: Group 1 reached an average necrotic area of 48.86%, Group 2 - 23.14%. After the statistic analysis, compared with the control group, Group 2 showed a statistically significant increase in survival area (p<0.001). CONCLUSION: The experimental model proved to be reliable to be used in the study of effects of low level laser therapy in random skin flap in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-01-01) Freitas, André Luiz Pires de [UNIFESP]; Silva, Marcelo de Oliveira e; Matsumoto, Priscilla Martin Keiko; Han, Sang Won [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS: Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS: obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION: It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Fator de crescimento do endotélio vascular na viabilidade do retalho musculofasciocutâneo transverso do reto do abdome, em ratos submetidos à nicotina
    (Universidade Federal de São Paulo (UNIFESP), 2009-07-30) Silveira, Tiago Santos [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Several factors can reduce the viability of the TRAM flap, among them the nicotine has been made responsible by the loss partial or total of these flaps. Objective: To evaluate the action of the Vascular endothelial Growth Factor in the viability of the Transverse Rectus Abdominis Musculocutaneous, in rats submitted to the nicotine. Methods: Sixty Wistar EPM-1 rats were used, adult males, weighing from 230 to 300g, randomized in 4 groups of 15 animals each: Group Control composed by animals that were submitted to the TRAM flap; Group Nicotine composed by animals that were nicotine exposed and submitted of TRAM flap; Group VEGF composed by animals submitted to the administration of VEGF plasmidial before the TRAM flap; and Group Nicotina+VEGF composed by animals that were exposed to the nicotine, trated with administration of VEGF and submitted to the TRAM flap. For analysis of the results they were done necrosis area and vascular density. Results: There was estatistic significant differentiates in the comparison among all of the groups, regarding the variables necrosis area and vascular density (p <0,05). Conclusion: The Vascular Endothelial Growth Factor increased the viability of the Transverse Rectus Abdominis Musculocutaneous, in rats submitted to the nicotine.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Histamine iontophoresis on the viability of random skin flap in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-02-01) Esteves Junior, Ivaldo [UNIFESP]; Tacani, Pascale Mutti [UNIFESP]; Liggieri, Victor Cicone; Ruggi, Bruno Grinman; Ferreira, Lydia Masako [UNIFESP]; Liebano, Richard Eloin; UNIP Physical Therapy Department; Mackenzie Presbyterian University; Universidade Federal de São Paulo (UNIFESP); University of Sao Caetano do Sul Physical Therapy Department; Sao Camilo University Center; UNIP; Universidade de São Paulo (USP)
    PURPOSE: To evaluate the effects of the histamine iontophoresis on the random skin flap viability in rats. METHODS: Sixty adult male Wistar rats were used. A cranially-based dorsal skin flap measuring 10 x 4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into four groups (G1-G4) (n=15 each group) as follows: G1 (control) - sham electrical stimulation, G2 (electrical stimulation) - direct current electrical stimulation, G3 (histamine) - histamine and sham electrical stimulation and G4 (histamine iontophoresis) - transdermal iontophoresis of histamine. In all groups the procedures were performed immediately after the surgery and on the two subsequent days. The percentage of flap necrosis was measured on the seventh postoperative day. RESULTS: The mean and the respective standard deviation of the percentage of flap necrosis areas were as follows: G1 (control) - 47.87 ± 9.13%, G2 - 51.49 ± 8.19%, G3 - 46.33 ± 8.32% and G4 - 30.82 ± 11.25%. The G4 group presented a significantly smaller amount of flap necrosis when compared to the other groups (p<0.001). CONCLUSION: The topical administration of the histamine by iontophoresis was effective to increase the viability of the random skin flaps in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Influence of minoxidil on ischemic cutaneous flaps in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2005-12-01) Bittencourt, Rogério De Castro; Biondo-simões, Maria De Lourdes Pessole [UNIFESP]; Paula, Josué Brunginski De; Martynetz, Juliano; Groth, Anne; Catholic University of Paraná; PUCPR Cajuru University Hospital; Brazilian Society of Plastic Surgery; Universidade Federal de São Paulo (UNIFESP); CBC; SOBRADPEC; Society of Plastic Surgery
    PURPOSE: To evaluate the influence of minoxidil, a well known vasodilator, on ischemic flap necrosis prevention in rats. METHODS: Ventral cutaneous flaps, measuring 8x4cm, were designed in 20 Wistar rats based on the right cranial epigastric artery. In the experiment group, 50 mg/kg/day of minoxidil sulfate was administered by orogastric tube and the same amount of saline solution was administered to the control group. Such procedure was initiated 24h before surgery and kept once a day through the 7th postoperative day. Microcirculation was evaluated with laser fluxometry 24h before surgical procedure, at immediate postoperative and at the 7th postoperative day. Flap necrosis area was evaluated by 2 methods: planimetry and weight/paper ratio. RESULTS: A significant flow increase in distal and medial extremity at M1 (medial point 1) (p=0,0484) was observed in the experiment group. There was significant difference in flap necrosis prevention in the experiment group (p=0,0433), although after necrosis took place there was no significant difference in necrosis size. (p=0,1051 and p=0,2799). CONCLUSION: Minoxidil sulfate is effective in avoiding necrosis in ischemic flaps, but after necrosis is present there is no difference in survival area between experimental and control groups.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Keratinized versus non-keratinized preputial flap onlay urethroplasty: does it make any difference in the histological analysis? An experimental study in rabbits
    (Brazilian Soc Urol, 2013-11-01) Costa, Marnio [UNIFESP]; Leslie, Bruno [UNIFESP]; Rondon, Atila [UNIFESP]; Bacelar, Herick [UNIFESP]; Mattos, Ricardo [UNIFESP]; Barbosa, Bruno [UNIFESP]; Delcelo, Rosana [UNIFESP]; Ortiz, Valdemar [UNIFESP]; Macedo, Antonio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose: To compare the histological characteristics of keratinized versus non-keratinized onlay island flaps in an experimental rabbit model.Materials and Methods: Sixteen male rabbits were randomly allocated into two experimental groups: keratinized and non-keratinized onlay island flaps. A defect was created in the ventral aspect of the penile urethra. in the keratinized group, a longitudinal island flap was harvested from the external prepuce and rotated to cover the urethral defect. in the non-keratinized group a transverse island flap was harvested from the inner prepuce. the animals were sacrificed after 2, 4, 8 and 12 weeks.Results: the flaps were viable in all animals, and no deaths were associated with the procedure. Two urethrocutaneous fistulas were identified, one in each experimental group. A similar pattern of fibrosis was identified in both groups. the keratinized epithelium of the external prepuce kept its histological aspect and keratin production. Both keratinized and non-keratinized groups presented a slight decrease on the epithelial thickness, however without a statistically significant difference between groups.Conclusions: in this short-term rabbit model, we observed that the stratified squamous keratinized epithelium from the external prepuce kept its keratin production. There was no statistical influence of the flap type on the mean epithelial thickness.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Nicotine on rat TRAM flap
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-06-01) Ely, Pedro Bins; Kobayashi, Ludmila Aimi [UNIFESP]; Campos, José Humberto Oliveira; Gomes, Heitor Carvalho [UNIFESP]; Juliano, Yara [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Unit Santa Casa de Porto Alegre Plastic Surgery; Universidade Federal de São Paulo (UNIFESP); Escola Bahiana de Medicina Department of Surgery Head of Operative Technique and Experimental Surgery Division
    PURPOSE: The transverse rectus abdominis myocutaneous (TRAM) flap is one of the preferential techniques used in breast reconstruction following mastectomy. Nicotine has a detrimental effect on cutaneous flap survival; although there are no experimental studies proving this effect on musculocutaneous flaps. The aim of this study is to verify the effect of nicotine on the rat TRAM flap. METHODS: 30 Wistar EPM-1 rats were randomly distributed in two groups: control and experimental. The animals of the control group received saline solution injected subcutaneously, in a volume of 0.2 ml, twice a day, during 28 days in the preoperative period. The animals of the experimental group were treated with nicotine, injected subcutaneously, in a dose of 2 mg/kg twice a day, during 28 days in the preoperative period. All the animals were submitted to the caudally based, right unipedicled TRAM flap. 48 hours after the procedure, a study of the viable area of the flap was done through photographic documentation. Statistical analysis was performed with nonparametric Mann-Whitney's test. RESULTS: The experimental group had significantly greater area of necrosis when compared with the control group (p<0.001). CONCLUSION: Nicotine increased the area of necrosis of the TRAM flap, in rats.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Oncoplastic approach in the conservative treatment of breast cancer: analysis of costs
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-05-01) Lima, Débora Eleotério De; Veiga Filho, Joel [UNIFESP]; Ribeiro, Leda Marques; Morais, Thiago Bezerra De; Rocha, Luiz Roberto Martins [UNIFESP]; Juliano, Yara; Veiga, Daniela Francescato [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; UNIVÁS Plastic Surgery Division; UNIVÁS Mastology Division; UNIVÁS Hospital das Clínicas Samuel Libânio Plastic Surgery Division; UNIVÁS School of Administration; UNIVÁS Bioestatistics Division; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To analyze the direct costs of conservative surgical treatment of breast cancer, performed in a university hospital, to the Brazilian National Health Care Public System (SUS), checking the impact of the oncoplastic approach on these costs. METHODS: One hundred thirty eight breast cancer patients who had undergone conservative treatment with oncoplastic approach (n=36) or not (control group, n=102), in the period from 2005 to 2010, were enrolled. Sociodemographic and clinical data were recorded. The direct costs of the surgical procedure were obtained and analyzed. RESULTS: Groups did not differ in regard to age (p=0.963), and patients in oncoplastic group had a longer time of hospital stay (p=0.000). The median direct cost for the oncoplastic group was R$461.00 and for the control group was R$229.00 (p=0.000). CONCLUSION: The oncoplastic approach has generated higher direct costs in conservative surgical treatment of breast cancer to SUS.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Peptídeo relacionado ao gene da calcitonina por iontoforese na viabilidade de retalho cutâneo randômico em ratos
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2004-12-01) Esteves Junior, Ivaldo [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Liebano, Richard Eloin [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Purpose: Assess the effect of the local administration of calcitonin gene-related peptide (CGRP) by iontophoresis on the viability of random skin flap in rats. Methods: Sixty Wistar-EPM 1 rats were submitted to dorsal skin flap of cranial base 10 x 4 cm with interposition of a plastic barrier between the skin flap and the donnor site. The animals were randomly distributed in four groups (n = 15 in each group) and were treated as follows: in group 1 (control), animals were submitted to a simulation of electrical current for 20 minutes; group 2, (iontophoresis placebo) animals were submitted to direct current of 4mA amplitude for 20 minutes; group 3 (absorption control) receive simulation of electrical current for 20 minutes with CGRP on one of the electrodes; group 4 (iontophoretically treated) treated by iontophoresis with CGRP. In all groups the procedures were performed immediately after the surgery and the two subsequent days. Results: The percentage of the necrosis area was calculated in all groups on the seventh postoperative day and results were as follows: group 1- 48%; group 2 - 51%; group 3 - 46% and group 4 - 28%. The statistical analysis presented significant difference when the comparison of group 4 to the other groups. Conclusion: The topical administration of the CGRP by iontophoresis is efficient to increase the viability of the random skin flap random in rats.
  • Imagem de Miniatura
    Item
    Embargo
    Qualidade de vida em pacientes submetidos a reconstrução mamária tardia com o retalho musculocutâneo transverso do reto abdominal
    (Universidade Federal de São Paulo (UNIFESP), 2002) Veiga, Daniela Francescato [UNIFESP]; Sabino Neto, Miguel [UNIFESP]; Universidade Federal de São Paulo [UNIFESP]
    Objetivo: Avaliar o impacto da reconstrucao mamaria tardia com o retalho musculocutaneo transverso do reto abdominal na qualidade de vida de 25 pacientes mastectomizadas, e a satisfacao geral delas com o resultado. Metodos: Para avaliar a qualidade de vida, foi utilizado o questionario generico de avaliacao de qualidade de vida SF-36, aplicado no pre e pos-operatorio de tres, seis e doze meses. A satisfacao das pacientes foi avaliada atraves de uma versao adaptada da subescala de satisfacao geral de Alderman et al., aplicada apos o primeiro ano pos-operatorio. Resultados: O impacto da reconstrucao mamaria na qualidade de vida das pacientes foi positivo em todos os dominios do SF-36I Houve diferenca estatisticamente significante, em relacao ao pre, nos dominios aspecto emocional e Saúde mental, no 3º mes pos-operatorio, e estado geral de Saúde, no 6º mes. No 12º mes posoperatorio houve melhora significante de todos os outros dominios, exceto vitalidade que, embora tambem tenha apresentado melhora, esta nao chegou a ser estatisticamente significante. Na subescala de satisfacao geral com o resultado, todas as pacientes demonstraram satisfacao em todos os itens. Conclusoes: a reconstrucao mamaria tardia com o retalho musculocutaneo transverso do reto abdominal promove um impacto positivo na qualidade de vida das pacientes, e o grau de satisfacao geral delas com o resultado e elevado
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Reconstrução mamária tardia com retalho músculo-cutâneo transverso do reto abdominal: avaliação dos resultados pelas pacientes e por dois cirurgiões plásticos
    (Universidade Federal de São Paulo (UNIFESP), 2001) Veiga, Daniela Francescato [UNIFESP]; Sabino Neto, Miguel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Com o objetivo de avaliar o grau de satisfação das pacientes e de dois observadores independentes, cirurgiões plásticos com experiência em reconstrução mamária, realizamos a reconstrução mamária tardia com o retalho musculocutâneo transverso do reto abdominal em 20 pacientes mastectomizadas, selecionadas consecutivamente nos ambulatórios de Cirurgia Plástica/Mastologia de dois hospitais universitários. A técnica cirúrgica utilizada foi a convencional, sendo 45% dos retalhos monopediculados e 55% bipediculados. Em todos os casos a cirurgia foi realizada em dois tempos, onde o segundo consistiu de simetrização e reconstrução do complexo aréolo-papilar. Os resultados foram avaliados e comparados três e seis meses após a conclusão da reconstrução, através de dois métodos: atribuição de notas de 0 a 10 pelas pacientes e pelos observadores ao resultado global da reconstrução e avaliação por categorias de sub-escalas (volume, forma e posicionamento da mama, sulco inframamário e cicatrizes) realizada pelos dois observadores. Não houve diferença significante entre as avaliações aos três e seis meses. Observamos concordância e satisfação com os resultados entre os observadores, e maior grau de satisfação com os resultados por parte das pacientes do que dos observadores, sendo que 95% das pacientes atribuíram nota máxima ao resultado.