Navegando por Palavras-chave "Stress, physiological"
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- ItemAcesso aberto (Open Access)Avaliação de modificações pós-traducionais de caráter redox do fungo Paracoccidioides brasiliensis após estresse nitrosativo(Universidade Federal de São Paulo (UNIFESP), 2017-03-31) Navarro, Marina Valente [UNIFESP]; Batista, Wagner Luiz [UNIFESP]; http://lattes.cnpq.br/4373797404389169; http://lattes.cnpq.br/9313460640378183; Universidade Federal de São Paulo (UNIFESP)The fungi Paracoccidioides brasiliensis and P. lutzii are the causative agents of paracoccidioidomycosis, a systemic mycosis endemic in Latin America. This fungus is considered a facultative intracellular pathogen, able to survive and replicate inside macrophages. Its survival during infection depends on the fungus adaptability to various conditions, such as nitrosative/oxidative stress produced by the host immune cells, particularly alveolar macrophages. Currently, there is few knowledge about the P. brasiliensis signaling pathways involved in the fungus evasion mechanism of the host defense response. However, it is known that some of these pathways are triggered by reactive oxygen and nitrogen species (ROS/RNS) produced by host cells. Considering that NO (nitric oxide) effects on pathogens is concentration dependent, such effects could alter the redox state of cysteine residues by influencing (activation or inhibition) a variety of protein functions, standing out S-nitrosylation, a very important post translational modification NO dependent which regulates cellular functions and signaling pathways. It has been demonstrated by our group that P brasiliensis yeast cells proliferate when exposed to low NO concentrations. Thus, this work investigated the modulation profile of S-nitrosylated proteins of P. brasiliensis, as well as identified S-nitrosylation sites after treatment with RNS. Through mass spectrometry analysis (LC-MS/MS) and label-free quantification it was possible to identify 487 proteins on global proteome study and 474 proteins on S-nitrosylated proteome study. With this approach, we observed that proteins treated with NO low concentrations presented a proliferative response pattern, with several proteins involved with cellular cycle regulation and growth and would represent, as last analysis, molecules with an important role to fungi virulence. On the other hand, proteins stimulated with NO high concentrations exhibited a survival response pattern and may help to understand RNS antifungal properties and identify potential molecular targets to future development of new drugs.
- ItemAcesso aberto (Open Access)Estresse e modos de andar a vida: subsídios de Georges Canguilhem para uma etnoepidemiologia da Síndrome Geral da Adaptação na cidade de São Paulo.(Universidade Federal de São Paulo (UNIFESP), 2010-09-29) Santos, Maurici Tadeu Ferreira dos [UNIFESP]; Gomes, Mara Helena de Andrea [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)This study investigated the perception that the residents of a condominium in the city of Sao Paulo have about their stress and life conditions, with the goal of establishing a critical reflection based on a ethnoepidemiologic proposal. We employed descriptive research on residential condominium called "Projeto Viver Celso Garcia – CPV” in the district of Belenzinho, east of the state capital, with contributions of anthropology (ethnography) and descriptive epidemiology as the main means for better understanding our object of study. The interviews allowed us to complement additional data collection; held with 16 residents and a key non-resident informant, using a questionnaire adapted from Peluso & Blay (2008), Lipp (1999), Holmes & Rahe ( 1976) and Sheldon Cohen et al (1983). The interviews were conducted during the second half of 2009 and the analysis and treatment in the first half of 2010. The responses were categorized and interpreted based on adapting the technique of "content analysis" in Pondé et al (2009) and Minayo (2007). The ethnography was based on the concepts of Georges Canguilhem in his book "The Normal and Pathological" (2009) and epidemiological data were based on research to databases from the SIAB - Information System of Primary Care. The approach allowed ethnoepidemiologic find in the multifaceted and ambiguous character of stress, a predominance of polarized feelings among the descriptors "frustrating and gratifying" showing different ways dependent on the ways of walking life. It also allowed us to see the stress as "faithfulness or unfaithfulness" associated with “individual organic whole – environment” according to the production of individual or collective norms that modulate ways of walking life following ideas of Georges Canguilhem. The study was suitable as a resource for better understanding of the need for capacity and production of norms in complex systems such as those which are involved on the general adaptation syndrome and adaptive disorders in urban conditions of living as well.
- ItemAcesso aberto (Open Access)Fatores estressores do paciente e necessidades do familiar convivente em unidade de terapia intensiva coronariana(Universidade Federal de São Paulo (UNIFESP), 2018-11-28) Coelho, Alue Constantino [UNIFESP]; Barros, Alba Lucia Bottura Leite de [UNIFESP]; http://lattes.cnpq.br/3089430786971948; http://lattes.cnpq.br/2602947152920298; Universidade Federal de São Paulo (UNIFESP)INTRODUCTION: The hospitalization at the Coronary Intensive Care Unit (ICU) generates stressful events and needs in patients and their families. The environment associated with the process of humanization is fundamental for the clinical stability, recovery, rehabilitation, wellbeing and satisfaction of family members and of the severe patients who need intensive care. Patient and Family Centered Care needs to be applied and valued, addressing the issues and needs of family members regarding patient care. OBJECTIVE: To evaluate the stressors of patients hospitalized in the Coronary Intensive Care Unit; to evaluate the need and satisfaction of family members living with patients hospitalized in a Coronary ICU. METHODS: It is an observational crosssectional study. The sample consisted of 200 participants, 100 patients with Acute Coronary Syndrome, after 24 hours of hospitalization in the Coronary ICU of Hospital São Paulo, and 100 relatives living with them. Data were collected from March 2016 to January 2018, after completing the inclusion criteria, presentation of the study and agreement of participation in the research. Firstly, the sociodemographic and clinical characterization form of the patient was applied followed by the scale of evaluation of stressors in an intensive care unit. At the time of the visit, the family sociodemographic characterization form and the inventory of needs and stressors of family members in intensive care INEFTI were applied. Stressors were analyzed and represented by the mean and standard deviation for each item and then compared. RESULTS: The majority of the patients are male, married, Catholic, retired, aged between 5160 years old, 1 st grade incomplete, autonomous, experiencing the first ICU stay, with two days in the unit, with a diagnosis of infarction Acute myocardial infarction with supra low level of the lower wall segment ST, with associated comorbidities, with arterial hypertension and dyslipidemia being the most prevalent, presenting complications such as Reverted Sudden Death and arrhythmia, classified at the time of admission with High Risk Grace Score and having their exit from the ICU with improved clinical condition and in outpatient followup. The most stressful factors of the patients were: "Having financial worries" (30%), "Not being able to communicate" (29%), "Not having control over themselves" (28%), "Being incapacitated to family "(27%) and" Not aware of the length of stay in the ICU "(27%). The cohabiting relatives are female, being children and spouses, married, Catholics, age between 1830 years, full second degree, employees, with monthly income of 1 to 3 Minimum Wages, with previous experience in ICU , aware of the diagnosis of the hospitalized patient, but not aware of the name of the doctor and the nurse responsible. Responses to the Inventory of Needs of Families in Intensive Care showed that family members were more important to their needs than they were satisfied. The most important needs were: to know the patient's chances of improvement, to be sure that the best possible treatment was being given to the patient, to be advised at home about changes in the patient's condition and to be informed about everything related to evolution of the patient. The need considered less satisfied by family members was to have a person who can give information over the phone. CONCLUSION: The stressors of the patient are related to their commitments, their responsibilities and their adaptation to their biopsychosocial evolution in the Coronary ICU and the satisfaction of their cohabiting relatives needs to be valued in front of their needs in search of good results. Therefore, it is necessary for professionals to extend the focus of care to the patient and the family, contemplating the process of humanization and the principles of Patient and Family Centered Care so that care is effective, safe and of quality, aiming at recovery and promotion the health of the patient and the prevention and satisfaction of his or her relative.
- ItemSomente MetadadadosInfluência do "Stress" agudo provocado pelo formol sobre o metabolismo da fibra cardíaca do rato albino: estudo histoquímico e ultra-estrutural(Universidade Federal de São Paulo (UNIFESP), 1978) Lopes, Antonio Carlos [UNIFESP]; Barcellini, Adolpho [UNIFESP]
- ItemSomente MetadadadosQualidade de vida, morbidade psiquiátrica e eventos estres sores de pacientes portadores de litíase renal com historia de cólicas recorrentes: um estudo caso-controle(Universidade Federal de São Paulo (UNIFESP), 2006) Diniz, Denise Helena de Madureira Pará [UNIFESP]; Schor, Nestor [UNIFESP]
- ItemAcesso aberto (Open Access)Sinais e sintomas da disfunção autônoma em indivíduos disfônicos(Sociedade Brasileira de Fonoaudiologia, 2011-01-01) Park, Kelly [UNIFESP]; Behlau, Mara [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To verify the occurrence of signs and symptoms of autonomic nervous system dysfunction in individuals with behavioral dysphonia, and to compare it with the results obtained by individuals without vocal complaints. METHODS: Participants were 128 adult individuals with ages between 14 and 74 years, divided into two groups: behavioral dysphonia (61 subjects) and without vocal complaints (67 subjects). It was administered the Protocol of Autonomic Dysfunction, containing 46 questions: 22 related to the autonomic nervous system and had no direct relationship with voice, 16 related to both autonomic nervous system and voice, six non-relevant questions, and two reliability questions. RESULTS: There was a higher occurrence of reported neurovegetative signs in the group with behavioral dysphonia, in questions related to voice, such as frequent throat clearing, frequent swallowing need, fatigability when speaking, and sore throat. In questions not directly related to voice, dysphonic individuals presented greater occurrence of three out of 22 symptoms: gas, tinnitus and aerophagia. Both groups presented similar results in questions non-relevant to the autonomic nervous system. Reliability questions needed reformulation. CONCLUSION: Individuals with behavioral dysphonia present higher occurrence of neurovegetative signs and symptoms, particularly those with direct relationship with voice, indicating greater lability of the autonomic nervous system in these subjects.
- ItemAcesso aberto (Open Access)Sinais e sintomas da disfunção autônoma em indivíduos disfônicos(Universidade Federal de São Paulo (UNIFESP), 2009-01-28) Park, Kelly [UNIFESP]; Behlau, Mara [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: The objective of this study is the occurrence of signs and symptoms of dysfunction of the autonomic nervous system in patients with behavioral dysphonia and compare the results with individuals without vocal complaints. Methods: A search of 128 adult subjects, categorized into 2 groups according to the presence of behavioral dysphonia (dysphonic group, 61 participants) and healthy voice (control group, 67 participants). The group with behavioral dysphonia was composed by 43 subjects were female and 18 male, aged between 14 and 66 years old, the healthy group with voice was composed of 45 women and 22 men, aged between 15 and 74 years. We applied the protocol of autonomic dysfunction (Demmink - Geertman & Dejonckere, 2002) containing 46 questions, distributed as follows: 22 related to the autonomic nervous system and no direct relationship with the voice, 16 related to both the autonomic nervous system and the voice, 6 points and 2 non-relevant questions of reliability. Results: There was a higher incidence of neurovegetative signs of changes in the group with behavioral dysphonia, especially in matters related to the voice (eight of 16 questions), and the higher occurrence of: frequent throat clearing (86.9%, N = 53, p <0001) , need to constantly swallowing (68.9%, N = 42, p <0001),fatigability when speaking (67.2%, N = 41, p <0001) and sore throat (65.6%, N = 40 , p <0011). As for symptoms neurovegetative without direct relationship with the voice, the individuals in the group with behavioral dysphonia had higher occurrence of three of the 22 symptoms: puffiness (85.2%, N = 52, p <0001), tinnitus (59%, N = 36, p = 0002) and aerophagia (57.4%, N = 35, p = 0003). Finally, on issues considered non-relevant, the groups behaved in a manner similar to them all. The consistency questions were excluded because of reliability problems in its formulation. Conclusion: individuals with behavioral dysphonia have a higher incidence of behavioral symptoms neurovegetative in general, particularly those who have direct relationship with the voice, indicating a greater lability of the autonomic nervous system.