Navegando por Palavras-chave "Skin aging"

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    Anti-wrinkle and anti-whitening effects of juca (Libidibia ferrea Mart.) extracts
    (Springer, 2016) Pedrosa, Tatiana do Nascimento; Barros, Aline Oliveira; Nogueira, Jessica Rodrigues; Fruet, Andrea Costa; Rodrigues, Isis Costa; Calcagno, Danielle Queiroz [UNIFESP]; Smith, Marilia de Arruda Cardoso [UNIFESP]; de Souza, Tatiane Pereira; de Moraes Barros, Silvia Berlanga; de Vasconcellos, Marne Carvalho; Araujo da Silva, Felipe Moura; Ferreira Koolen, Hector Henrique; Maria-Engler, Silvya Stuchi; Lima, Emerson Silva
    Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as juca, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of juca trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 +/- 0.8 A mu g/mL) and LFP (IC50 = 16 +/- 0.5 A mu g/mL), was stronger than standard rutin (IC50 = 27.6 +/- 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.
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    Avaliação do colágeno dérmico no envelhecimento pela ultrassonografia de alta frequência e espectroscopia Raman in vivo e suas correlações com a histologia
    (Universidade Federal de São Paulo (UNIFESP), 2017-03-29) Caetano, Livia de Vasconcelos Nasser [UNIFESP]; Bagatin, Edileia [UNIFESP]; Miot, Hélio Amante; Martin, Airton Abrahão; http://lattes.cnpq.br/2543633050941005; http://lattes.cnpq.br/7541422056269063; http://lattes.cnpq.br/6478900066830476; http://lattes.cnpq.br/2907081841741428; Universidade Federal de São Paulo (UNIFESP)
    Background: The analysis of the correlation between noninvasive dermal collagen evaluation techniques, such as high frequency ultrasonography and Raman spectroscopy, with skin biopsy – often considered the gold standard - is essential to support the results of dermatological studies. Objectives: to characterize and describe the high frequency ultrasound and the Raman spectroscopy parameters associated to dermal collagen in intrinsically aged and photoaged skin; and to analyze their correlation with histology. Methods: a cross sectional diagnostic assessment intervention study included 23 women between 28 and 82 years from the general outpatient clinic of the department of Dermatology of the Federal University of São Paulo. They were divided into 3 groups according to the right forearm photoaging score as mild (1), moderate (2) or advanced (3). Analysis of the dermis on photoexposed (forearm back) and non-photoexposed area (medial arm) was carried out through three methods: high frequency ultrasound, confocal Raman spectroscopy and skin biopsy for immunohistochemistry (collagen I) and histological assessment using picrosirius-red and Verhöff staining techniques together with digital image analysis of histological and ultrasound pictures. The differences between the photoexposed and non-photoexposed regions were studied and statistic correlations between each of the non-invasive methods and the age, photoaging score and histological parameters were assessed. Results: at the non-photoexposed site, the upper and lower dermis ultrasound parameter ratios better correlated with age. The higher coefficients were for the ratio of the low echogenic pixels between the upper and lower dermis, the ratio of the medium and high echogenic pixels between the upper and lower dermis and the ratio of total intensity between the upper and lower dermis. There was a slight correlation with collagen I area fraction in immunohistochemistry. At the photoexposed area, total and upper dermis parameters demonstrated higher correlations with age and clinical score. The low echogenicity pixels area fraction, medium and high echogenic pixels area fraction and Total Intensity presented the higher coefficients. The correlation coefficients between ultrasound parameters and histology were higher for the photoexposed site compared with the non-photoexposed region. The medium and high echogenic pixels area fraction and Total Intensity presented the higher correlation coefficients with collagen area fraction in the Picrossírius red staining (direct) and with solar elastosis area fraction in Verhöff staining (inverse). Principal Component Analysis of the dermis Raman spectra was able to discriminate the photoexposed region (photoaging) from the non-photoexposed region (intrinsic aging) in group 3; and the non-photoexposed dermis of group 1 from group 3 (intrinsic aging). The peak intensities in 855cm-1 and 938cm-1 showed moderate correlation with age, high-frequency ultrasonography, and immunohistochemistry for Collagen I in the non-exposed region. In the photoexposed region, the ratio between 1275 cm-1 and 1450 cm-1 intensities (I1275/I1450) presented moderate to high correlation with age, photoaging score, dermis echogenicity and histology (immunohistochemistry for collagen I, picrossirius and Verhöff). Conclusion: a moderate to high correlation between histology and ultrasound was found in the photoexposed region. Lower, but significant, correlation coefficients occurred in the non-photoexposed region. Significant correlations were also found between in vivo Raman spectroscopy, histology and ultrasonography. The 938 cm-1 and 855 cm-1 peak intensities may be useful for intrinsic aging assessment, while I1275/I1450 ratio can be used for photoaging measurement.
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    Bases biomoleculares do fotoenvelhecimento
    (Soc Brasileira Dermatologia, 2009-05-01) Montagner, Suelen; Costa, Adilson [UNIFESP]; PUC SP; HMMG; PUCCAMPINAS; Universidade Federal de São Paulo (UNIFESP)
    As a result of the increase in life expectancy, the study of the organic process of aging has been stimulated. Skin ageing, which reflects the signs of time, is a time-dependent process of progressive deterioration that can be intensified by sun exposure, which is known as photoaging. The damage of radiation on various cell structures and on the skin results in molecular and morphological changes to these components. Many research studies are performed to try to minimize the effects of photoaging; however, the main strategy to manage it is still prevention, which will only be achieved once we learn about the mechanisms involved in the process.
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    Efeito da emulsão clássica de 5-fluorouracil versus 5-fluorouracil manipulado em emulsão com homogeneização de alta velocidade no fotoenvelhecimento avançado e no campo de cancerização dos antebraços
    (Universidade Federal de São Paulo, 2024-05-15) Mestnik, Natalia Cammarosano [UNIFESP]; Bagatin, Ediléia [UNIFESP]; Miot, Hélio Amante; http://lattes.cnpq.br/2543633050941005; http://lattes.cnpq.br/6478900066830476; http://lattes.cnpq.br/5706811993228935
    Fundamentos: O fotoenvelhecimento refere-se à associação de alterações biológicas secundárias à exposição solar sobre o envelhecimento intrínseco. A degeneração causada pela radiação ultravioleta (UV) sobre diversas estruturas celulares e moleculares determina diversas alterações cutâneas, entre elas, o câncer de pele. As queratoses actínicas (QAs) múltiplas, uma das características do fotoenvelhecimento avançado, são consideradas lesões pré neoplásicas e constituem o chamado campo de cancerização (CC). Fotoenvelhecimento avançado e CC são fenômenos cutâneos que se desenvolvem paralelamente. O uso tópico de 5-Fluorouracil (5-FU) é aprovado para o tratamento de QAs. No entanto, suas limitações de uso estão associadas aos efeitos colaterais como eritema, descamação, crostas e prurido. Atualmente, o desenvolvimento de medicações tópicas empregando novas tecnologias, como a homogeneização de alta velocidade, visa melhor ou igual eficácia das formulações, com menos eventos adversos. Objetivos: 1) Avaliar a eficácia, perfil de segurança e tolerabilidade através dos efeitos clínicos e histológicos epidérmicos e dérmicos do 5-FU a 5% em emulsão tópica manipulada com homogeneização de alta velocidade (fórmula inovadora), comparada à emulsão tradicional no tratamento do CC e no fotoenvelhecimento avançado dos antebraços de pacientes imunocompetentes. 2) Analisar marcadores envolvidos no CC por meio da análise de imuno-histoquímica e expressão gênica. Métodos: Estudo clínico de fase II de intervenção terapêutica, randomizado, prospectivo, comparativo e duplo-cego. Após aprovação pelo Comitê de Ética em Pesquisa – Unifesp, assinatura do Termo de Consentimento Livre e Esclarecido (TCLE) e do Termo de Autorização para uso de imagens, foram incluídos 41 participantes com fotoenvelhecimento grave ou avançado dos antebraços (classificação de Guimarães, 2015) com QAs múltiplas. O esquema terapêutico compreendeu o uso do 5-FU 5% em emulsão tradicional em um antebraço (grupo Clássico) e em emulsão preparada com homogeneização de alta velocidade no antebraço contralateral (grupo Turrax), definido por randomização, duas vezes ao dia, durante quatro semanas. A avaliação da eficácia incluiu os seguintes parâmetros: 1) Clínicos: avaliação do investigador, através da Escala de Fotoenvelhecimento dos Antebraços (EFA) e contagem de QAs; e de dois observadores independentes por comparação de fotografias padronizadas; 2) Medidas instrumentais não invasivas: corneometria, cutometria e ultrassonografia (USG) de alta frequência. Todas as medidas foram realizadas antes (T0) e após 12 semanas do início do tratamento (T12); 3) Biópsias de pele em T0 e T12, uma em cada antebraço para exames histológico, imuno-histoquímico e análise da expressão gênica e outra biópsia em T0 na região ventral do braço esquerdo em área não fotoexposta para comparação das vias associadas ao envelhecimento extrínseco e intrínseco (controle). A avaliação de segurança baseou-se na observação e relatos de efeitos colaterais. Resultados: Dos 41 participantes incluídos 40 completaram o estudo. Os achados clínicos confirmaram os benefícios do 5-FU 5% tópico, independentemente da formulação utilizada: 1. EFA (média grupo Turrax: T0=100/T12=88, p<0,01; média grupo Clássico: T0=101/T12=87, p<0,01; sem diferença entre os tratamentos); 2. Contagem de QAs (média grupo Turrax: T0=8/T12=0, p<0,001; média grupo Clássico: T0=9/T12=0, p<0,001; sem diferença entre os tratamentos); assim como as medidas não invasivas: corneometria (média grupo Turrax: T0=27,7/T12=33,1, p<0,01; média grupo Clássico T0=29,5/T12=32,6, p=0,04; sem diferença entre os tratamentos); cutometria (número amostral de 19, mediana R7 grupo Turrax T0=0,29/T12=0,51, p<0,01; mediana R7 grupo Clássico T0=0,27/T12=0,47, p<0,01, sem diferença entre os tratamentos); USG: SLEB (subepidermal low echogenic band) (n amostral de 19, Média Grupo Turrax T0=0,36/T12=0,36, p = 0,97; Média Grupo Clássico T0=0,39/T12=0,43, p = 0,21; com diferença entre eles p =0,04). Os achados histológicos (número amostral de 21) demonstraram melhora da classificação da neoplasia intraepidérmica de queratinócitos (KIN) e a tendência da redução da expressão imuno-histoquímica de p53 e Ki-67 epidérmicas em T12 em ambos os grupos. Os efeitos colaterais observados pelo investigador (eritema, descamação, prurido, ressecamento e crostas) ocorreram em todos os casos, em ambos os antebraços, porém, foram de intensidade leve a moderada e não apresentaram diferença estatística entre os tratamentos utilizados. Conclusão: Os tratamentos tópicos com 5-FU a 5% em creme ou preparado com homogeneização de alta velocidade foram eficazes no tratamento do CC e na melhora dos sinais de fotoenvelhecimento dos antebraços.
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    In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment
    (Elsevier Ireland Ltd, 2017) Nasser Caetano, Livia de Vasconcelos [UNIFESP]; Mendes, Thiago de Oliveira; Bagatin, Edileia [UNIFESP]; Miot, Helio Amante; Marques Soares, Juliana Laudiceia [UNIFESP]; Enokihara, Milvia Maria Simoes e Silva [UNIFESP]; Martin, Airton Abrahao
    Background: In vivo confocal Raman spectroscopy is a non-invasive method to assess either the epidermis or the dermis composition. Few studies have focused on dermis collagen alterations through intrinsic aging and photoaging. Objective: This study evaluated the in vivo Raman spectra from the dermis of a photoexposed site versus a non-photoexposed region in different age groups, and evaluated the correlation between peak intensities and age, photoaging score and the amount of collagen assessed with histology and high frequency ultrasound (HFUS). Methods: Fifteen volunteers aged 28-82 years were divided into three groups according to forearm photoaging degree. In vivo Raman spectra from the dermis were collected on the dorsal forearm (chronically photoexposed skin) and on the proximal medial arm (non-photoexposed skin). Crosssectional images of the skin were obtained using a 20 MHz ultrasound unit exactly on the same sites, which were further submitted to punch biopsies for histologic study (collagen I immunohistochemistry, picrosirius red staining and Verhoeff). Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) were taken in the spectral region of 796 cm(-1) -996 cm(-1) to determine its potential to discriminate between different groups. The Spearman rank correlation coefficient of individual peak intensities and ratios with age, clinical score and the amount of collagen assessed by ultrasound and histology were calculated. Results: PCA of pairs of groups and OPLS-DA could discriminate the intrinsically from the photoaged skin and the young group from the elderly one, with important contribution of the 938 cm(-1) and 855 cm(-1) peaks intensities. The intensity of the peaks in 855 cm(-1) and/or 938 cm(-1) presented moderate correlation with age (rho = 0.579, p = 0.049) and moderate to high inverse correlation with HFUS echogenicity (rho = -0.710, p = 0.010) and collagen I immunohistochemistry (rho = -0.833, p = 0.005) in the non-photoexposed region. The I-1275/I-1450 intensities ratio presented moderate to high correlation coefficients with age (rho = -0.730, p = 0.007), photoaging score (rho = -0.594, p = 0.042), HFUS echogenicity (rho = 0.760, p < 0.001) and histology (collagen I immunohistochemistry (rho = 0.643, p = 0.024), picrosirius (rho = 0.773, p = 0.005) and Verhoeff (rho = -0.727, p = 0.011)) in the photoexposed site. Conclusion: The wavenumber region between 798 and 994 cm(-1) is useful for the analysis of dermal collagen alterations through the intrinsic aging process, while photoaging is better assessed by the I-1275/I-1450 intensities ratio. This is the first skin aging study to show a correlation between Raman peaks and the amount of collagen assessed by HFUS- and histology. (C) 2017 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
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    Luz intensa pulsada e ácido 5 aminolevulínico no tratamento de queratoses actínicas e fotoenvelhecimento facial
    (Universidade Federal de São Paulo (UNIFESP), 2010-04-28) Haddad, Alessandra [UNIFESP]; Santos, Ivan Dunshee de Abranches Oliveira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    BACKGROUND: Photodynamic rejuvenation is the term used to the treatment of actinic keratosis and photodamage with photodynamic therapy. OBJECTIVE: Evaluate the efficacy of photodynamic therapy with aminolevulinic acid and intense pulsed light in the treatment of actinic keratosis and photodamage. METHODS: Fourty three patients mean age 71,3 years, were randomized in three groups: Control (IPL 18J); Treatment I (ALA+ IPL 16J); and Treatment II ( ALA+ IPL 18J) and evaluated clinically an photographically by two observers in Pre, 48hs, 8 weeks and 12 weeks Post-Treatment using Griffth’s Photonumeric Scale. Five actinic keratosis were marked as target in Pre-Treatment and biopsies Pre and Post-Treatment were done in one lesion to evaluate atypias and loss of cellular polarity. RESULTS: Non parametric tests showed marked improvement in mottled pigmentation, texture fine wrinkles in Groups Treatment I and II. In Control Group improvement only in mottled pigmentation. Actinic Keratosis clearance in Groups Treatment I and II were 51,2% and 68,4% respectively, whereas in Group Control it was 5%. In relation to Control Group, Groups Treatment I and II showed significant reduction of atypias and improvement in loss of cellular polarity. CONCLUSIONS: Photodynamic therapy with aminolevulinic acid and intense pulsed light is effective in the treatment of photodamage and actinic keratosis. The resolution of actinic keratosis was better with higher levels of energy.
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    Microbiota e envelhecimento da pele humana
    (Universidade Federal de São Paulo, 2023-06-16) Mariano, Estella de Souza Nascimento [UNIFESP]; Lopes, Patricia Santos [UNIFESP]; http://lattes.cnpq.br/7939687315116927; http://lattes.cnpq.br/1427123023166925
    Essencial para sobrevivência, a pele constitui uma barreira física, microbiológica e imunológica entre o meio externo e interno que, em consequência de danos sofridos ao longo do tempo, sofre modificações. As rugas são uma das principais modificações morfológicas que ocorrem devido ao envelhecimento cutâneo que é ocasionado por fatores intrínsecos e extrínsecos. Dentre eles, a radiação UV é uma das principais responsáveis pela degradação da matriz extracelular e o aparecimento deste fenótipo do envelhecimento. Aliado a radiação, a disbiose do microbioma da pele e intestinal (eixo intestino-pele) ocasiona um declínio da homeostase colaborando para a aparição desses vincos que demonstram uma melhora através da aplicação de probióticos, prebióticos, simbióticos e pós-bióticos que previnem e diminuem sua aparição. Devido a uma maior procura e interesse em proporcionar uma pele mais saudável e equilibrada, estudos acerca de cosméticos com microrganismos aumentaram demonstrando sua eficácia no combate contra as rugas, além da existência da relação eixo intestino-pele. Mesmo com o aumento de estudos clínicos e a inovação das análises do microbioma cutâneo, há necessidade de mais pesquisas para evidenciar os mecanismos e benefícios dos probióticos e afins, e de regulamentar a utilização dos mesmos para garantir a segurança do consumidor. Esta revisão buscou discutir e apresentar a relação entre a formação das rugas através do envelhecimento da cútis, sua relação com o microbioma cutâneo e intestinal, os efeitos benéficos que os probióticos e correlatos ocasionam nos mecanismos do envelhecimento e no aparecimento de rugas, e as dificuldades e problemas relacionados a utilização de microrganismos em produtos cosméticos.
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    Non-ablative radiofrequency associated or not with low-level laser therapy on the treatment of facial wrinkles in adult women: A randomized single-blind clinical trial
    (Taylor & Francis Inc, 2017) Pereira, Thalita Rodrigues Christovam [UNIFESP]; Vassão, Patricia Gabrielli [UNIFESP]; Venancio, Michele Garcia [UNIFESP]; Renno, Ana Claudia Muniz [UNIFESP]; Aveiro, Mariana Chaves [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objectives: The objective of this study was to evaluate the effects of Non-ablative Radiofrequency (RF) associated or not with low-level laser therapy (LLLT) on aspect of facial wrinkles among adult women. Methods: Forty-six participants were randomized into three groups: Control Group (CG, n = 15), RF Group (RG, n = 16), and RF and LLLT Group (RLG, n = 15). Every participant was evaluated on baseline (T0), after eight weeks (T8) and eight weeks after the completion of treatment (follow-up). They were photographed in order to classify nasolabial folds and periorbital wrinkles (Modified Fitzpatrick Wrinkle Scale and Fitzpatrick Wrinkle Classification System, respectively) and improvement on appearance (Global Aesthetic Improvement Scale). Photograph analyses were performed by 3 blinded evaluators. Results: Classification of nasolabial and periorbital wrinkles did not show any significant difference between groups. Aesthetic appearance indicated a significant improvement for nasolabial folds on the right side of face immediately after treatment (p = 0.018) and follow-up (p = 0.029) comparison. RG presented better results than CG on T8 (p = 0.041, ES = −0.49) and on follow-up (p = 0.041, ES = −0.49) and better than RLG on T8 (p = 0.041, ES = −0.49). RLG presented better results than CG on follow-up (p = 0.007, ES = −0.37). Conclusion: Nasolabial folds and periorbital wrinkles did not change throughout the study; however, some aesthetic improvement was observed. LLLT did not potentiate RF treatment.
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    An overview about oxidation in clinical practice of skin aging
    (Soc Brasileira Dermatologia, 2017) Silva, Silas Arandas Monteiro e [UNIFESP]; Michniak-Kohn, Bozena; Leonardi, Gislaine Ricci
    Free radicals are unstable chemical species, highly reactive, being formed by cellular entities of different tissues. Increased production of these species without proper effective action of endogenous and exogenous antioxidant systems, generates a condition of oxidative stress, potentially provider of skin disorders that extend from functional impairments (skin cancer, dermatitis, chronic and acute inflammatory processes) even aesthetic character, with the destruction of structural proteins and cellular changes with the appearance of stains, marks and lines of expressions and other signs inherent to the intrinsic and extrinsic skin aging process. The antioxidants are chemical substances commonly used in clinical practice for topical application and may contribute in the fight against the radical species responsible for many skin damage. This paper summarized the main evidence of the benefits brought by the topical application of antioxidants in the skin, considering the amplitude of the indicative performance of antioxidant activity by in vitro and ex-vivo tests as well as in vivo tests. It is recognized that a breadth of product performance tests should be explored to truly identify the effectiveness of antioxidant products for an anti-aging effect.
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    Radiofrequência não ablativa associada ou não à terapia a laser de baixa intensidade no tratamento de rugas faciais em mulheres adultas
    (Universidade Federal de São Paulo (UNIFESP), 2016-04-19) Pereira, Thalita Rodrigues Christovam [UNIFESP]; Aveiro, Mariana Chaves [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objectives: To evaluate the effects of nonablative Radiofrequency (RF) associated ou not with Low Level Laser Therapy (LLLT) onaspect of facial wrinkles among adult women, and the impact of both of themon female self-esteem. Methods: This was a controlled clinical trial, randomized and blind.46 women between 30 and 50 years old presenting I-IV Fitzpatrick skin phototype and II-III Glogau degrees of aging were included. Participants were randomized between 3 groups: Control Group (CG), Nonablative Radiofrequency Group (RG) and Nonablative Radiofrequency and Low Level Laser Therapy Group (RLG). CG was only evaluated, RG received four sessions of nonablative radiofrequency (RF), RLG received four sessions of RF and 24 sessions of low level laser therapy (LLLT).The baseline (T0) consisted of general data, medical and dermatologic history, assessment of skin type using the Roberts Skin Type Classification System and evaluation of self-esteem through the Brazilian version of the Rosenberg Self-Esteem Scale. The participants were re-evaluated after eight weeks of the initial assessment (T8) and eight weeks after T8 (follow up). Each woman was photographed in order to assess the degree of nasolabial and periorbital wrinkles (Modified Fitzpatrick Wrinkle Scale and The Fitzpatrick Wrinkle Classification System, respectively) and the improvement in appearance through the Global Aesthetic Improvement Scale. Photograph analyzes were performed by an independent evaluation commission who had no knowledge of the allocation of the participants in the study groups. Results: Nasolabial and periorbital wrinkles classification did not show significant improvement between groups throughout the study. Analysis of aesthetic improvement indicated a significant improvement for nasolabial folds on the right side immediately after treatment (T0vs. T8, p=0.018) and follow up (T0 vs. followup, p=0.029) comparison. RG presented better results than CG immediately after treatment (p=0.041, effect size=-0.49) and on follow up (p=0.041, effect size=-0.49) and than RLG for immediately after treatment (p=0.041, effect size=-0.49). RLG presented better results than CG on follow up (p=0.007, effect size=-0.37). GR improved self-esteem during treatment (p=0.040). Conclusion: There was no significant difference in the classification of nasolabial and periorbital wrinkles between the groups throughout the study; however, it was observed some Global Aesthetic Improvement.
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    Randomized clinical efficacy of superficial peeling with 85% lactic acid versus 70% glycolic acid
    (Sociedade Brasileira de Dermatologia, 2013-12-01) Prestes, Paula Souza; Oliveira, Márcia Motta Maia de; Leonardi, Gislaine Ricci [UNIFESP]; Methodist University of Piracicaba Health Sciences College; aff02; Universidade Federal de São Paulo (UNIFESP)
    BACKGROUND: Peeling is a procedure which aims to accelerate the process of skin exfoliation. OBJECTIVES:Development of formulations containing lactic acid at 85% or glycolic acid at 70% and the evaluation of these formulations on clinical efficacy in reduction of fine wrinkles.METHODS:Preliminary stability tests were carried out and an in vivo study was performed with three groups with 9 representatives each. One was the control group, which used only sunscreen; another one used lactic acid+sunscreen, and the last group used acid glycolic+sunscreen. Clinical efficacy was assessed with a CCD color microscope, through the digitization of images before and after treatment. The applications were carried out by a dermatologist, once a mont h every 30 days, during 3 months. The area with wrinkles was calculated by planimetry point counting, in accordance with Mandarin-de-Lacerda.RESULTS:The formulations were stable in the visual and Ph evaluation. There was no improvement in the control group; for lactic acid, there was significant improvement after the second peeling application on the outer lateral area of the right eye and after the third application on the outer lateral area of the left eye. For the glycolic acid group, there was significant improvement in the outer lateral area of the left eye after the first application, and of the right eye region, after three applications. The formulations used must be kept under refrigeration and should be manipulated every 30 days.CONCLUSIONS:Both peelings were effective in reducing fine wrinkles of the outer lateral eye area after three applications (p≤0.05%). It was observed that peeling efficacy in the external-lateral region of one eye might be different compared with that in skin of the external-lateral region of the other eye, relative to the speed of skin improvement.
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    Tretinoin peel: a critical view
    (Soc Brasileira Dermatologia, 2017) Sumita, Juliana Mayumi; Leonardi, Gislaine Ricci; Bagatin, Edileia
    The tretinoin peel, also known as retinoic acid peel, is a superficial peeling often performed in dermatological clinics in Brazil. The first study on this was published in 2001, by Cuce et al., as a treatment option for melasma. Since then, other studies have reported its applicability with reasonable methodology, although without a consistent scientific background and consensus. Topical tretinoin is used for the treatment of various dermatoses such as acne, melasma, scars, skin aging and nonmelanoma skin cancer. The identification of retinoids cellular receptors was reported in 1987, but a direct cause-effect relation has not been established. This article reviews studies evaluating the use of topical tretinoin as agent for superficial chemical peel. Most of them have shown benefits in the treatment of melasma and skin aging. A better quality methodology in the study design, considering indication and intervention is indispensable regarding concentration, vehicle and treatment regimen ( interval and number of applications). Additionally, more controlled and randomized studies comparing the treatment with tretinoin cream versus its use as a peeling agent, mainly for melasma and photoaging, are necessary.