Navegando por Palavras-chave "Single-cell gel (comet) assay"

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    Chronic renal failure induces genetic instability in multiple organs of Wistar rats
    (Wiley-Blackwell, 2009-04-01) Ribeiro, Daniel Araki [UNIFESP]; Campos, Ruy Ribeiro [UNIFESP]; Bergamaschi, Cassia Toledo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Taking into consideration the strong evidence for a relationship between DNA damage and carcinogenesis, the aim of this study was to investigate whether blood, liver, heart, kidney and brain are particularly sensitive organs for DNA damaging during chronic renal disease by the single-cell gel (comet) assay to predict genetic instability induced by this pathological condition.A total of 18 male Wistar rats were divided into two groups: negative control (n = 8) and experimental (n = 10), in which was submitted to the 5/6 renal mass ablation by ligation of two or three branches of the left renal artery and total right nephrectomy during 8 weeks.The results showed that chronic renal disease was able to induce genetic damage in blood, heart, liver and kidney cells as depicted by the mean tail moment. No genetic damage was induced in brain cells, i.e. no significant statistically differences (P > 0.05) were noticed when compared to negative control.In conclusion, our results suggest that chronic renal failure could contribute to the damage of DNA at all organs evaluated, except to the brain cells. As DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risks associated with kidney disease.
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    Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro
    (Springer, 2012-06-01) Carlin, Viviane [UNIFESP]; Matsumoto, Mariza A.; Saraiva, Patricia P.; Artioli, Andre; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); USC
    The aim of the present study was to comparatively evaluate DNA damage and cellular death in cells exposed to various commercially available mouthrinses: ListerineA (R) CepacolA (R), Plax alcohol freeA (R), PeriogardA (R), and Plax WhiteningA (R). A total of 75 volunteers were included in the search distributed into five groups containing 15 people each for in vivo study. Exfoliated buccal mucosa cells were collected immediately before mouthrinse exposure and after 2 weeks. Furthermore, blood samples were obtained from three healthy donors for in vitro study. the micronucleus test was used to evaluate mutagenicity and cytotoxicity in vivo. the single-cell gel (comet) assay was used to determine DNA damage in vitro. After 2 weeks exposure, PeriogardA (R) showed 1.8% of micronucleated cells with significant statistical differences ( < 0.05) compared to before exposure (0.27%). Plax WhiteningA (R) presented high tail moment value (4.5) when compared to negative control (0.6). the addition of all mouthrinses to cells incubated with methyl methanesulfonate did not alter the number of strand breaks in the genetic material. ListerineA (R) was able to reduce genetic damage induced by hydrogen peroxide because a decrease of tail moment was noticed. the results of the present study suggest that PeriogardA (R) and Plax WhiteningA (R) can induce genetic damage, whereas ListerineA (R) is an antioxidant agent. Since DNA damage is considered to be prime mechanism during chemical carcinogenesis, these data may be relevant in risk assessment for protecting human health and preventing carcinogenesis.
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    Exercise preconditioning modulates genotoxicity induced by doxorubicin in multiple organs of rats
    (Wiley-Blackwell, 2012-06-01) Martins, Renato Almeida [UNIFESP]; Minari, André Luis Araújo [UNIFESP]; Chaves, Marcelo Donizetti [UNIFESP]; Santos, Ronaldo Vagner Thomatieli dos [UNIFESP]; Barbisan, Luis Fernando; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    The aim of this study was to investigate the effects of exercise in multiple organs of rats treated with doxorubicin. Male adult Wistar rats were distributed into the following groups: sedentary + NaCl; exercise + NaCl; sedentary + doxorubicin; and exercise + doxorubicin. Animals were sacrificed 2?days following injections. Central fragments from heart, liver, and kidney were collected and minced in 0.9% NaCl being cellular suspensions used for the single-cell gel (comet) assay. the results showed that exercise was able to prevent genotoxicity induced by doxorubicin in heart cells. By contrast, exercise was not able to prevent genotoxicity induced by doxorubicin in liver cells. the same occurred to kidney cells, i.e. no statistically significant differences (p?>?0.05) were found when compared with groups not exposed to doxorubicin. Taken together, our results support the idea that exercise could contribute to the protective effect against genotoxicity induced by doxorubicin in heart cells. Copyright (c) 2012 John Wiley & Sons, Ltd.
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    Genetic damage in multiple organs of acutely exercised rats
    (Wiley-Blackwell, 2010-12-01) Pozzi, Renan [UNIFESP]; Rosa Neto, José Cesar [UNIFESP]; Eguchi, Ricardo [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Aguiar, Odair [UNIFESP]; Chaves, Marcelo Donizetti [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to investigate the effects of acute exercise on genomic damage in an animal model. Male adult Wistar rats were divided into the following groups: control and acute exercised (experimental). for this purpose, 15 animals were accustomed to running on a rodent treadmill for 15 min per day for 5 days (10-20 m min(-1); 08 grade). After 4 days at rest, active animals ran on the treadmill (22 m min(-1), 58 grade) till exhaustion. Cells from peripheral blood, liver, heart, and brain were collected after 0, 2, and 6 h after exercise. the results showed that acute exercise was able to induce genetic damage in peripheral blood cells after 2 and 6 h of exercise, whereas liver pointed out genetic damage for all periods evaluated. No genetic damage was induced either in brain or in heart cells. in conclusion, our results suggest that acute exercise could contribute to the genetic damage in peripheral blood and liver cells. It seems that liver is a sensitive organ to the genotoxic insult after acute exercise. Copyright (C) 2010 John Wiley & Sons, Ltd.