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- ItemSomente MetadadadosAchados histopatológicos e expressão da glicoproteina-P em retinoblastoma(Universidade Federal de São Paulo (UNIFESP), 2005) Souza Filho, João Pessoa de [UNIFESP]; Martins, Maria Cristina [UNIFESP]Objetivos: Investigar a expressão da glicoproteína-P (P-gp) em retinoblastoma em espécimes enucleados primariamente ou pós-tratamento conservador e correlacionar essa expressão com achados histopatológicos. Métodos: Foi realizada uma análise retrospectiva dos espécimes obtidos consecutivamente entre os anos de 1993 a 2003 por enucleação primária (grupo I) ou após falência do tratamento conservador (grupo II). O grau de diferenciação do grupo I foi classificado de acordo com a porcentagem de rosetas de Flexner-Wintersteiner. Os retinoblastomas do grupo II foram divididos em: tumor com células de aspecto viável, regressão com componente bem diferenciado e regressão tumoral. Outros achados como invasão da coróide e nervo óptico foram também avaliados. A expressão da P-gp foi graduada sem iquantitativamente, como negativa, baixa ou forte. As variáveis foram analisadas estatisticamente pelo teste qui-quadrado e teste t de Student. Resultados: No grupo I, 65 por cento dos tumores eram moderadamente diferenciados, 30 por cento bem diferenciados e 5 por cento pouco diferenciados. Quinze por cento apresentavam apenas invasão do nervo óptico, 20 por cento apenas invasão da coróide e 55 por cento mostravam simultaneamente invasão do nervo óptico e coróide. O grupo II apresentou 62,5 por cento de tumores bem diferenciados, 25 por cento com regressão total do tumor e 12,5 por cento dos tumores apresentavam células viáveis pouco diferenciadas. Cerca de 18 por cento apresentavam invasão apenas da coróide, 6,3 por cento invasão apenas do nervo óptico e 6,3 por cento mostravam simultaneamente invasão nervo óptico e coróide. A expressão da P-gp foi observada em 60 por cento do grupo I e 66,6 por cento do grupo II (diferença não estatisticamente significante), sendo observada positividade da reação em 81,2 por cento dos tumores bem diferenciados e 53,8 por cento dos tumores moderadamente diferenciados. A diferença da expressão da P-gp entre os tumores bem diferenciados em relação aos tumores moderadamente e/ou pouco diferenciados foi estatisticamente significante (p=0,028). Conclusões: A expressão da P-gp foi mais freqüente em tumores bem diferenciados, em especial, nos casos previamente tratados com quimioterapia, que podem estar relacionados à falência terapêutica.
- ItemAcesso aberto (Open Access)Achados histopatológicos em retinoblastoma(Conselho Brasileiro de Oftalmologia, 2005-06-01) Souza Filho, João Pessoa de [UNIFESP]; Martins, Maria Cristina [UNIFESP]; Torres, Virgínia Laura [UNIFESP]; Dias, Ana Beatriz Toledo [UNIFESP]; Lowen, Marcia Serva [UNIFESP]; Pires, Luciana Afonso [UNIFESP]; Erwenne, Clélia Maria [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)OBJETIVO: To study histopathological findings of enucleated eyes with retinoblastoma. METHODS: Twenty-eight cases of retinoblastoma treated by enucleation at the Federal University of São Paulo from December 2000 to October 2002 were histopathologically reviewed. Clinical data included age, gender, race, unilateral or bilateral involvement and previous treatment. The histopathological review evaluated the presence of iris and/or angle neovascularization, tumor differentiation and optic nerve and choroidal invasion according to Khelfaoui's classification. RESULTS: Of 27 patients, 13 (48.5%) were boys and 14 (59.3%) were girls, 16 were white, 6 were black and 5 were asiatic, age ranging from 2 to 96 months (mean, 22.7 months). 13 cases were bilateral and 14 cases were unilateral. All tumors were histologically characterized by a proliferation of small cells with high nuclear-to-cytoplasmic ratios and 20 (71.4%) of them were well differentiated. Choroidal involvement was observed in 18 (64.2%) cases (degree II, III) and optic nerve invasion in 8 (28.5%) cases (degree III, IV, V). CONCLUSION: Neovascularization, necrosis and calcification were the most commonly observed feature. The invasion into the optic nerve and choroid, which are the two most important predictors of patient outcome were found in 28.5% and 64.2% of the cases, respectively.
- ItemSomente MetadadadosAnálisis inmunohistoquímico del fenotipo celular del retinoblastoma utilizando marcadores de células neurales y gliales(Universidade Federal de São Paulo (UNIFESP), 2015-03-31) Alonzo, Maria Eugenia Orellana de [UNIFESP]; Burnier Júnior, Miguel Noel Nascente [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Retinoblastoma is the most common intraocular malignant tumor in childhood yet its origin remains unclear. It has been shown that retinoblastoma cells express both mature and immature retinal markers. An immunohistochemical analysis of the cell origin of this particular tumor may yield valuable insight into disease progression. This study aims to determine the origin of retinoblastoma in a large case series and correlate these findings with histopathological prognostic factors. Methods: Thirty nine retinoblastoma cases were histopathologicaly diagnosed and further analyzed by immunohistochemistry using monoclonal antibodies against immature retinal cell marker (SOX-2: SRY-box containing gene 2), mature retinal cell marker (MAP2: microtubule-associated protein 2), and glial cell marker (GFAP: glial fibrillary acidic protein). Histopathological features, including pattern of growth, differentiation, vitreous seeding, choroidal/scleral, optic nerve, and anterior chamber invasion, were evaluated. Two retinoblastoma cell lines were studied using immunocytochemistry staining, with the same markers. Results: Expression of SOX-2 was strong in 97.4% of retinoblastoma cases, while MAP-2 was expressed in 59% of cases. Immunostaining for GFAP was consistent across all cases; positive exclusively in reactive stromal astrocytes interspersed amongst tumor cells. There was no correlation between histopathological prognostic factors and the immunohistochemical markers. Retinoblastoma cell lines showed strong positivity for SOX2 (WERI-1cells 90% and in 70% of Y79 cells). MAP2 was positive in WERI-1 cells and Y79 cells (90%). GFAP was completely negative in both cell lines. Conclusion: These results suggest that the majority of retinoblastomas and the RB cell lines expressed immature rather than mature retinal cell markers. Therefore, these results indicate a neural origin of this particular tumor. In addition, the absence of GFAP positivity in tumor cells further suggests a retinal cell origin of retinoblastoma.
- ItemAcesso aberto (Open Access)Avaliação audiológica em crianças com retinoblastoma(Universidade Federal de São Paulo, 2021-12) Rodrigues, Gabriela Borges [UNIFESP]; Gil, Daniela [UNIFESP]; Macedo, Carla Renata Pacheco Donato; http://lattes.cnpq.br/0245855985770902; http://lattes.cnpq.br/6363626867862971; http://lattes.cnpq.br/4266320594768905Introdução: O retinoblastoma – tumor maligno intraocular da retina, apesar de considerado o mais comum dentre os cânceres na infância, consiste em uma doença relativamente rara, afetando aproximadamente um em cada 16.000 a 18.000 nascidos vivos da população global. O tratamento medicamentoso contra tumores é potencialmente prejudicial aos sistemas auditivo e vestibular, devido à ototoxicidade dos fármacos utilizados, em sua maioria, derivados da platina – cisplatina e carboplatina. Esses têm como consequência, não só a morte de células ciliadas da cóclea, atingindo a princípio sua base, mas também grande potencial de acometimento da porção central da audição. Assim, devido à possibilidade de se afetarem estruturas da via de audição, faz-se necessária a avaliação do processamento auditivo central. Objetivo: Caracterizar a avaliação comportamental do processamento auditivo central em crianças e adolescentes fora de tratamento de retinoblastoma tratadas com a carboplatina. Método: Avaliou-se dez crianças de 5 anos a 18 anos que passaram pelo tratamento de retinoblastoma há pelo menos dois anos. Os pacientes foram submetidos à avaliação comportamental do processamento auditivo central, constituída pelos testes: Teste de Localização Sonora, Teste de Memória Sequencial para Sons Verbais, Teste de Memória Sequencial para Sons Não Verbais, Teste de Fala com Ruído Branco, Teste Dicótico de Dígitos, Teste de Identificação de Sentenças Sintéticas (Mensagem Competitiva Ipsilateral), Teste de Padrão de Duração, Teste Dicótico Consoante-Vogal (Atenção Livre) e Teste de Identificação de Intervalos Aleatórios. Resultados: Observou-se na amostra, com a análise estatística, idade média de 10,53 anos, média da idade de diagnóstico de 1,57 anos, duração do tratamento de 8,8 meses e o tempo de remissão de 7,59 anos. Na avaliação comportamental do processamento auditivo central, alguns testes mostraram-se alterados na maioria dos indivíduos, por exemplo, o teste de memória sequencial de sons não verbais com 4 sons, o dicótico de dígitos, o consoante-vogal e o RGDT, todos com 50% ou mais de alteração nas avaliações realizadas. Quanto aos processos gnósicos alterados, 90% apresentou alteração de decodificação, 60% de organização, 50% de codificação e 40% de não verbal. Conclusão: Concluímos que crianças e adolescentes tratados de retinoblastoma com carboplatina apresentam Transtorno do Processamento Auditivo Central (TPAC). O TPAC envolve prioritariamente os processos gnósicos de decodificação, seguido de organização, codificação e, por fim, não verbal.
- ItemSomente MetadadadosClinical presentation and genetic paradigm of diffuse infiltrating retinoblastoma: a review(Acta Cirurgica Brasileira, 2016) Traine, Peter G.; Schedler, Katharina J.; Rodrigues, Eduardo B. [UNIFESP]Retinoblastoma is the most common childhood cancer. Thanks to modern technology and good medical access, mortality in Europe has decreased to about 5%. Diffuse infiltrating retinoblastoma is a very rare subtype of this neoplasm and is characterized by its atypical growth pattern. Diffuse infiltrating retinoblastoma may mimic other more innocuous diseases and may therefore be misdiagnosed. The purpose of this paper was to provide a short review of the main symptoms of diffuse infiltrating retinoblastoma presenting to the ophthalmologist and give a comparison to typical retinoblastoma. The second purpose was to set up a discussion of the genetic paradigm of diffuse infiltrating retinoblastoma. It has often been described to occur sporadically
- ItemAcesso aberto (Open Access)Construção e validação de álbum seriado sobre retinoblastoma(Universidade Federal de São Paulo (UNIFESP), 2018-06-28) Soares, Juliana Dos Santos [UNIFESP]; Pettengill, Myriam Aparecida Mandetta [UNIFESP]; http://lattes.cnpq.br/3111422210593688; http://lattes.cnpq.br/9564724662463213; Universidade Federal de São Paulo (UNIFESP)Introduction: Investigations about the coping experience of families of children diagnosed with retinoblastoma evidence the importance of the information, considered as a right that must be considered by healthy professionals. However, there is a lack of educational material on this subject for use by the child’s family. Objective: Develop and validate an educational material for the family of children with retinoblastoma. Method: a methodological study was conducted. The Patient and Family Centered Care Model was used as a theoretical reference to guide the preparation of the material. The study was conducted in three stages: the first, the educational material was developed, through a field study carried out in a center specialized in the treatment of childhood and youth cancer, in the city of São Paulo, with the aim to identify the information needs of families, and a bibliographic study to guide the theoretical content of the educational material. The second, a semantic and content validation was performed by an Expert Committee, in which the Delphi technique was applied; and the third stage was conducted to understand the family’s experience with the use of the educational material. Semistructured interview was carried out with families of children receiving retinoblastoma treatment at the same institute. Qualitative Content Analysis was used to data analysis. Results: an educational material named Retinoblastoma Serial Album was constructed, based on family´s information need, and validated through expert’s consensus. Three analytical categories emerged from the understanding of the family´s experience with the use of the material: “Fear of diagnosis and treatment”, “Family actions to deal with the situation” and “The serial album as a resource for the family to face the experience”. Conclusion: The Retinoblastoma Serial Album was built and validated for use with families of children with retinoblastoma and considered as a potential information strategy to attend the families of these children and facilitate the coping process in a dignified and strengthened way.
- ItemAcesso aberto (Open Access)Correlação entre a citologia e a histologia nas lesões intra-oculares suspeitas de malignidade(Conselho Brasileiro de Oftalmologia, 2003-06-01) Corrêa, Zélia Maria da Silva [UNIFESP]; Irion, Luciane Dreher; Augsburger, James J.; Schneider, Susan; Erwenne, Clélia Maria [UNIFESP]; Santa Casa de Porto Alegre-RS Serviço de Oftalmologia Setor de Oncologia Oftalmológica; Universidade Federal de São Paulo (UNIFESP); Laboratório Patologistas Reunidos; Universidade de Cincinnati Departamento de Oftalmologia; Universidade de Cincinnati Laboratório de Patologia OcularPURPOSE: To correlate the cytologic findings of fine-needle aspiration biopsy (FNAB) of intraocular tumors with the histopathology specimen when enucleation or tumor resection was the treatment of choice. METHODS: Retrospective analysis of 51 patientssubmitted tofine-needle aspiration biopsy for diagnostic purposes or cyto-histologic correlation. Exclusion criteria were non-solid lesions, conservative treatment and ultrasound guided biopsies. After exclusions, 20 patients with intraocular solid lesions suspected to be malignant were studied, 12 of them were females, with ages between 2 and 78 years. All biopsies were performed by the first author under direct (operating microscope) or indirect (binocular indirect ophthalmoscope) visualization. The chosen route was transaqueous for anterior segment tumors and transvitreous through the sclera for posterior segment tumors except cases with suspicion of retinoblastoma biopsied through the peripheral cornea. Two samples were obtained from different areas of the tumor, in all cases with a 25-gauge needle. After fine-needle aspiration biopsy, samples were sent for processing, fixation, and staining with the Papanicolaou and hematoxylin-eosin (HE) methods. Histology specimens were stained with hematoxylin-eosin. RESULTS: Three cases consisted of anterior segment (iris) tumors and 17 of tumors situated in the posterior segment, 3 of them retinoblastomas. Nine patients were biopsied for diagnostic purposes and 11 for cytological-histological correlation after enucleation. In only two cases, fine-needle aspiration biopsy yielded insufficient material for diagnosis that subsequently proved to be a granuloma and a melanoma. CONCLUSIONS: Fine-needle aspiration biopsy seems to be a reliable procedure based on cytological-histological correlations in the studied group.
- ItemSomente MetadadadosA esterase D como marcador genetico-bioquimico do retinoblastoma(Universidade Federal de São Paulo (UNIFESP), 1988) Costanzi-Strauss, Eugenia [UNIFESP]; Silva-Fernandes, Maria Eugênia da [UNIFESP]
- ItemSomente MetadadadosEstudo da funcao retiniana apos tratamento sistemico e local de retinoblastoma(Universidade Federal de São Paulo (UNIFESP), 2003) Motono, Marcia [UNIFESP]Objetivos: Determinar, pelo registro do eletrorretinograma de campo total, os possiveis efeitos na funcao retiniana, causados por agentes terapeuticos sistemicos e locais usados no tratamento do retinoblastoma. Metodos: Foram incluidos neste estudo 41 olhos de 38 criancas com media de idade = 7,6 anos, alocados em quatro grupos: Grupo I: olhos tratados com radioterapia por feixe-externo e tratamentos locais, associados ou nao a quimiorreducao (N=8); Grupo II- olhos contra-laterais de pacientes que se submeteram a quimiorreducao para tratamento do olho afetado (N=7); Grupo III: olhos tratados por quimiorreducao e tratamentos locais (N=10) e Grupo IV: olhos controle de 16 voluntarios normais (N=16).0 eletrorretinograma foi registrado seguindo a padronizacao da International Society for Clinical Electrophisiology of Vision (ISCEV). Os parametros eletrorretinograficos de amplitude pico a pico (gV) e tempo de culminacao da onda-b (ms) foram analisados pelo Teste Kruskal-Wallis e em seguida pelo Teste de Tukey post-hoc. Resultados: Dos 25 olhos com retinoblastoma, 24 apresentaram alteracoes eletrorretinograficas. Houve diferenca estatisticamente significante da amplitude na resposta escotopica de bastonetes (x2(3) = 28,824; p<0,001); na resposta escotopica maxima (x2(3) = 29,330; p<0,001); nos potenciais oscilatorios (x2(3) = 27,309; p<0,001); na resposta fotopica de cones obtida por flash isolado (x2(3) = 27,589; p<0,001) e no estimulo flicker (x2(3) = 28,204; p<0,001). Houve prolongamento estatisticamente significante no tempo de culminacao somente para a resposta fotopica de cones obtida por flash isolado (x2(3) = 12,974; p=0,005) e para o estimulo flicker (x2(3) = 20,154; p<0,001). Do total de olhos de pacientes com retinoblastoma, o unico olho com resultados normais, pertencia ao grupo de olhos normais remanescentes (Grupo II). A acuidade visual foi diferente nos 4 grupos (x2(3) = 23,966; p<0,001) e pior nos olhos irradiados. Conclusoes: A funcao retiniana, avaliada pelo eletrorretinograma de campo total, de olhos com retinoblastoma submetidos a tratamento sistemico elou local mostrou-se alterada em 96 por cento dos olhos estudados. As alteracoes encontradas foram a reducao na amplitude e prolongamento no tempo de culminacao. Os olhos irradiados foram os que mostraram as anormalidades eletrorretinograficas mais severas. Os olhos contralaterais de portadores de retinoblastoma unilateral, submetidos a quimiorreducao foram os menos comprometidos
- ItemAcesso aberto (Open Access)Estudo do raspado de conjuntiva de pacientes tratados de retinoblastoma: Etapa II -- citologia(Conselho Brasileiro de Oftalmologia, 2002-06-01) Santos, Maria Carmen Menezes; Santos, Gilda Da Cunha [UNIFESP]; Vozza, João Antônio; Medeiros, Nivaldo; Erwenne, Clélia Maria [UNIFESP]; Fundação Antônio Prudente Hospital A. C. Camargo; Universidade Federal de São Paulo (UNIFESP); Fundação Antônio Prudente Hospital A. C. Camargo Laboratório IPACE; Fundação Antônio Prudente Hospital A. C. Camargo Departamento de Patologia ClínicaPurpose: Human conjunctival cytology has been extensively studied. Lack of data about the characteristics of conjunctival smears in patients treated for retinoblastoma who underwent enucleation and radiotherapy motivated this study. Methods: A previously studied group of 114 orbits of 58 patients was examined. The authors present the results of conjunctival cytology in 4 different situations, with and without enucleation, with and without radiotherapy. Results: In the leukocytic infiltrate in orbits with ocular bulbi predominated the lymphocyte, while in the others predominated the polymorphonuclear neutrophil. In general, the majority of epithelial cell alterations seen in the conjunctival smears of irradiated orbits were: cell with large nuclei, prominent nucleoli, and cytoplasmatic hyalinization. Conclusion: The cytological findings of the conjunctival smears do not indicate a characteristic and distinct pattern for each group.
- ItemAcesso aberto (Open Access)Estudo do raspado de conjuntiva e margem palpebral de pacientes tratados de retinoblastoma: etapa I - Microflora aeróbica(Conselho Brasileiro de Oftalmologia, 2002-03-01) Santos, Maria Carmen Menezes; Santos, Gilda Da Cunha [UNIFESP]; Vozza, João Antônio; Medeiros, Nivaldo; Erwenne, Clélia Maria; Universidade Federal de São Paulo (UNIFESP); Fundação Antônio Prudente Hospital A. C. Camargo Laboratório IPACE; Fundação Antônio Prudente Hospital A. C. Camargo Departamento de Patologia Clínica; Fundação Antônio Prudente Hospital A. C. Camargo Serviço de OftalmologiaPurpose: Conjunctival discharge is a common complaint among patients treated for retinoblastoma who underwent enucleation and radiotherapy. Lack of data about the microflora in those patients motivated this study. Methods: One hundred and fourteen orbits of 58 patients were examined. The authors present the results of aerobic bacterial and fungal culture of conjunctival and eyelid margin smears, as well as conjunctival cytology, in 4 different conditions: with and without enucleation, with and without radiation. Microorganisms were identified according to the National Committee for Clinical Laboratory Standards, using Gram and Leishman staining. Results: Microorganisms were more frequent in irradiated anophthalmic sockets, both in the conjunctiva (54.5%) and eyelid margin (63.6%); however the difference was not statistically significant. On the other hand, in orbits with ocular bulbi, the radiation, in the late phase, represented a limiting factor for the presence of microorganims in the conjunctiva and eyelid margin, but without a statistically significant difference. The coagulase-negative Staphylococcus was the most frequent microorganism in the conjunctiva and eyelid margin in all groups. There was fungus growth, Candida tropicalis, in only one case. Conclusions: Topical antibiotics in patients treated for retinoblastoma who underwent enucleation, with or without radiotherapy, are suggested.
- ItemAcesso aberto (Open Access)Expressão imuno-histoquímica da proteína C-kit no Retinoblastoma(Universidade Federal de São Paulo (UNIFESP), 2009-09-30) Santos, Leticia Rielo de Moura [UNIFESP]; Burnier Júnior, Miguel Noel Nascente [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: C-kit is a transmembrane tyrosine kinase protein thought to play an important role in tumorigenesis. With the development of the compound Imatinib Mesylate which specifically inhibits tyrosine kinase receptors, C-kit has emerged as a potential therapeutic target. This study aims to determine the immunoexpression of C-kit in retinoblastoma and correlate this expression with histopathological prognostic features. Methods: Eighty-four paraffin-embedded enucleation globes of retinoblastoma were collected from the archives of the Henry C. Witelson Ocular Pathology Registry. C-kit immunostaining was used according to the protocol provided by Ventana Medical System Inc., Arizona. Immunoreactivity was correlated with the presence or absence of invasion into the choroid and optic nerve and the degree of tumour defferentiation. Odds ratios were calculated to quantify differences in C-kit expression between tumours with different patterns of invasion. Results: C-kit expression was identified in 33/63 specimens analysed (53.8%).Two of 13 tumours without choroidal or optic nerve invasio (15.4%) were positive for C-kit. C-kit expression was seen in 31 of the 50 tumours with extraretinal invasion (62%, p<0.01), 26 of 44 specimens with choroidal involvement (59.9%, p<0.02), and 20 of the 29 with optic nerve involvement (68.96%, p<0.02). Fourteen of 25 moderate or well-diferentiated specimens (56%) and 19 of 38 undifferentiated specimens (50%) displayed positivity for C-kit (p>0.5). Conclusions: More than half of Retinoblastomas in this study expressed C-kit. The expression of Ckit in these retinoblastomas strongly correlated with histopathological features of worse prognosis including optic nerve and choroidal invasion.
- ItemSomente MetadadadosHereditary diffuse infiltrating retinoblastoma(Cuba Editora, 2016) Schedler, Katharina J. E.; Traine, Peter G.; Lohmann, Dietmar R.; Haritoglou, Christos; Metz, Klaus A.; Rodrigues, Eduardo Buchele [UNIFESP]Retinoblastoma is one of the most common childhood cancers. The diffuse infiltrating retinoblastoma is a rare subtype of this neoplasm. The majority of cases of diffuse infiltrating retinoblastoma are unilateral and occur sporadically. Herein we report on a family with three children affected by retinoblastoma, among them one girl with diffuse infiltrating retinoblastoma. This girl was diagnosed at the age of 8 years with a unilateral diffuse infiltrating retinoblastoma. By contrast, the two brothers became clinically apparent in the first 2 years of life with bilateral retinoblastoma. The parents were clinically unremarkable. Genetic analysis of RB1 gene was performed. The girl with diffuse infiltrating RB was found to be heterozygous for an oncogenic mutation in the RB1 gene that was also carried by both brothers and the father of the family. These results show that diffuse infiltrating retinoblastoma can develop on the background of a hereditary predisposition to retinoblastoma.
- ItemSomente MetadadadosImmunohistochemical analysis of pdgfr-alpha, pdgfr-beta and c-abl in retinoblastoma: potential therapeutic targets(Karger, 2016) Sanft, Debra-Meghan; Worme, Mali Dawn; de Moura, Leticia Rielo [UNIFESP]; Zoroquiain, Pablo; Fernandes, Bruno F.; Antecka, Emilia; Burnier, Miguel N., Jr.Background: Our laboratory previously reported that imatinib mesylate (IM) has an inhibitory effect on two retinoblastoma (Rb) cell lines in vitro. Aims: The purpose of this project was to determine the immunoexpression of platelet-derived growth factor receptor (PDGFR)-alpha, PDGFR-beta and c-Abl in 61 human samples of Rb to determine if IM-sensitive receptors are present. Additionally, this paper seeks to establish a correlation between the expression of PDGFR, c-Abl and the histopathological prognosis. Methods: Sixty-one paraffin-embedded Rbs were collected from the Henry C. Witelson Ocular Pathology Registry. PDGFR-alpha, PDGFR-beta and c-Abl immunostaining was performed according to the protocol provided by Ventana Medical System Inc. Immunoreactivity was correlated with the presence or absence of invasion into the choroid and optic nerve. Results: Overall, c-Abl expression was identified in 50 out of 61 specimens (81.97%), PDGFR-alpha was identified in 20 out of 60 specimens (33.33%) and PDGFR-beta expression was identified in 57 out of 61 specimens (93.44%). Histopathological prognosis was not correlated with immunoreactivity except in the case of PDGFR-beta. Conclusions: Rb is a cancer that expresses PDGFR-alpha, PDGFR-beta and c-Abl, which are known targets of IM. These markers may be responsible for the documented therapeutic effect of IM on Rb cell lines. (C) 2016 S. Karger AG, Basel
- ItemSomente MetadadadosA importância da relação entre a evolução natural do retinoblastoma e a idade, sexo e raça dos pacientes, a origem genética, o estadio e tipo celular dos tumores(Universidade Federal de São Paulo (UNIFESP), 1993) Arce, Carlos G [UNIFESP]; Bonomo, Pedro Paulo de Oliveira [UNIFESP]
- ItemAcesso aberto (Open Access)Indications for eye removal over a 13-year period at an ophthalmology referral center in Sao Paulo, Brazil(Consel Brasil Oftalmologia, 2017) Hime, Bernardo; Isenberg, Jordan; Rocha, Gustavo; Lowen, Marcia [UNIFESP]; Morales, Melina [UNIFESP]; Fernandes, Bruno Franco; Belfort, Rubens N. [UNIFESP]Purpose: To analyze the indications and types of eye removal at Latin America's highest-volume ophthalmic reference center in Sao Paulo, Brazil. Methods: A retrospective analysis was conducted of surgical pathological reports in the electronic database of the Ophthalmology Department of the Universidade Federal de Sao Paulo Hospital of patients who underwent eye-removal procedures between January 2000 and December 2012. Results: A total of 412 cases met the inclusion criteria for this study. The most common indications for eye removal were ocular melanoma and retinoblastoma, representing 35.4% and 31.1% of the total cases, respectively. Other frequent indications included endophthalmitis (6.3%), nonspecific inflammation (4.1%), squamous cell carcinoma (3.6%), panophthalmitis (3.4%), and phthisis bulbi (1.2%). The remaining indications accounted for 14.8% of all cases, with each accounting for <1% of the total cases. Enucleation was the most common eye-removal procedure seen in our study. Conclusions: The two most common indications for eye removal were ocular melanoma and retinoblastoma. Alternative treatment options are effective in limiting the need for eye removal but are constrained by our public center's socioeconomic context.
- ItemSomente MetadadadosInvasao do nervo optico e das paredes do globo ocular no retinoblastoma: valor da tomografia computadorizada(Universidade Federal de São Paulo (UNIFESP), 2005) Ferme, Andrea Langone [UNIFESP]
- ItemAcesso aberto (Open Access)Phacoemulsification and foldable acrylic IOL implantation in children with treated retinoblastoma(Conselho Brasileiro de Oftalmologia, 2012-10-01) Tartarella, Marcia Beatriz [UNIFESP]; Britez-Colombi, Gloria Fátima [UNIFESP]; Motono, Marcia; Chojniak, Martha Motono; Fortes Filho, João Borges [UNIFESP]; Belfort, Rubens Junior [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Hospital A C Camargo Ocular Oncology Department; Universidade Federal do Rio Grande do Sul Department of OphthalmologyPURPOSE: To study the results of cataract surgery in children with radiation-induced cataract after treatment for retinoblastoma. METHODS: Retrospective interventional case series. Six consecutive patients diagnosed with secondary cataracts due to radiation therapy for retinoblastoma. Intervention: Phacoemulsification and foldable acrylic intraocular lens implantation. Outcomes measu- red: Visual acuity, binocular indirect ophthalmoscopy and slit-lamp biomicroscopy. Aspirated lens material and aqueous humor samples were collected during surgery. RESULTS: Six uniocular children between 3 to 5 years of age at time of surgery were studied. The mean time interval between radiotherapy and cataract diagnosis was 22.3 months. The mean follow-up after surgery was 17.2 months (range: 12 to 23 months). All eyes achieved a clear visual axis after surgery allowing monitoring of the tumor status. None developed recurrence or retinoblastoma dissemination. Histopathological analysis of the aspired material showed no tumoral cells in all samples. All patients improved vision after cataract surgery. CONCLUSIONS: Phacoemulsification with acrylic intraocular lens implantation seems to be a safe, feasible, and effective method for the removal of radiation-induced cataracts in patients with treated retinoblastoma.
- ItemRestritoQuimioterapia intra-arterial no tratamento do retinoblastoma: experiência de um centro de referência(Universidade Federal de São Paulo, 2022-12-08) Marques, Márcio Chaves Pedro [UNIFESP]; Abdala, Nitamar [UNIFESP]; http://lattes.cnpq.br/8132921767941082; http://lattes.cnpq.br/3320834247986405Objetivo: descrever as características demográficas e técnicas da nossa série de casos e avaliar a eficácia e a segurança da quimioterapia intra-arterial no tratamento do retinoblastoma nas diferentes vias arteriais de acesso. Métodos: análise retrospectiva de todos os pacientes com retinoblastoma intraocular, submetidos ao tratamento endovascular em um centro de neurorradiologia intervencionista entre janeiro de 2012 e novembro de 2019. Os dados demográficos, os tipos de retinoblastoma, as drogas utilizadas, as vias de acesso arterial, a taxa de enucleação do globo ocular e as complicações perioperatórias foram registrados e compilados. Resultados: foram realizados quinhentos e seis ciclos de quimioterapia intra-arterial em 113 pacientes, totalizando 134 olhos tratados. O cateterismo ostial da artéria oftálmica foi realizado em 370 ciclos (73%) havendo refluxo para a artéria carótida interna em 152 (30%). A taxa de preservação do globo ocular foi de 72%. A taxa de complicação foi de 7,11%. A complicação mais grave foi a ocorrência do reflexo cardiorrespiratório autonômico, presente em 1,97% dos ciclos. Conclusão: nesta série, a quimioterapia intra-arterial para o tratamento do retinoblastoma intraocular mostrou eficácia e segurança nas vias arteriais utilizadas.
- ItemSomente MetadadadosRelação genético-clínica em retinoblastoma(Universidade Federal de São Paulo (UNIFESP), 1987) Erwenne, Clélia Maria [UNIFESP]; Pacheco, José Carlos Gouvea [UNIFESP]