Navegando por Palavras-chave "Reperfusion Injury"
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- ItemAcesso aberto (Open Access)Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-04-01) Lee, Edwin Jin Su [UNIFESP]; Silva, Sônia Maria da [UNIFESP]; Simões, Manuel de Jesus [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)PURPOSE: Evaluate the effect of N-acetylcysteine in liver remnant after hepatectomy associated to ischemia-reperfusion injury in mice. METHODS: Male adult BALB/c mice, weighing 20-22g were used. Animals were anesthetized with ketamine (70 mg/kg) and xylazine (10 mg/kg); received N-acetylcysteine (150 mg/kg, H-IR-NAC group) or vehicle (H-IR group). Surgical procedures were performed under 10X magnification. Partial hepatectomy (30%) was followed by ischemia-reperfusion injury (30 minutes of ischemia and 60 minutes of reperfusion). Blood sample and liver tissue were removed before animal was euthanized. AST and ALT were evaluated in blood samples and histomorphological analyses were performed in remnant liver. Groups were compared by Mann-Whitney test, and it was considered significant when p<0.05. RESULTS: Biochemical evaluations showed reduced levels of ALT in NAC group (H-IR-NAC=376±127U/l vs H-IR=636±39U/l, p=0.023). AST was similar (p=0.456). H-IR group showed hepatic tissue with preserved architecture, large area of steatosis, vascular congestion and rare mitogenic activity. NAC group showed hepatic tissue with small area of steatosis, vascular congestion and elevated mitogenic activity, evidenced by increased binuclear cells (H-IR-NAC=15.88±0.52 vs H-IR=7.4±0.37, p<0.001). CONCLUSION: N-acetylcysteine promotes enzymatic and morphological protection against hepatectomy and ischemia-reperfusion injury.
- ItemAcesso aberto (Open Access)The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline(Acta Cirurgica Brasileira, 2017) Ribeiro de Oliveira, Teresinha Regina; de Oliveira, Geraldo Ferreira; Simoes, Ricardo Santos; Feitosa, Suellen Maurim [UNIFESP]; Tikazawa, Eduardo Hiroshi; Monteiro, Hugo Pequeno [UNIFESP]; Fagundes, Djalma Jose [UNIFESP]; Taha, Murched Omar [UNIFESP]Purpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+ R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes). Results: The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P< 0.05) compared to IR+ SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P> 0.05). The NOS-2 expression (qRTPCR) in the IRPTX group (P< 0.05) was higher than the values for the IPC+ IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P< 0.05), the IR-SS (P< 0.05) and the IR-PTX (P< 0.05) groups. Conclusions: The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect.
- ItemAcesso aberto (Open Access)Expression of oxidative stress and antioxidant defense genes in the kidney of inbred mice after intestinal ischemia and reperfusion(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12-01) Teruya, Roberto [UNIFESP]; Ikejiri, Adauto Tsutomu [UNIFESP]; Somaio Neto, Frederico [UNIFESP]; Chaves, José Carlos [UNIFESP]; Bertoletto, Paulo Roberto; Taha, Murched Omar [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Mato Grosso do Sul Federal University Surgery Department; Grande Dourados Federal University Medical School; UFGD Medical SchoolPURPOSE: To determine the gene expressions profile related to the oxidative stress and the antioxidant response in the kidneys of mice subjected to intestinal ischemia and reperfusion. METHODS: Twelve inbred mice (C57BL/6) were randomly assigned to one of two groups: the control group (CG) underwent anesthesia and was observed for 120 min and the ischemia/reperfusion group (IRG), animals were anesthetized and subjected to laparotomy and ischemia for 60 minutes followed by 60 minutes of reperfusion. The expressions of 84 genes from the kidney were determined by the Reverse Transcription qualitative Polymerase Chain Reaction (RT-qPCR). All genes that were up regulated by more than threefold using the algorithm [2(ΔΔCt)] were considered statically significant (p<0.05). RESULTS: In the IRG group 29 (34.52%) of 84 genes, were up regulated by more than threefold. The genes that were differentially up regulated in the glutathione peroxidase cluster (10 genes): were Gpx2 and Gpx7. The genes that were up regulated in the peroxidase cluster (16 genes) were following: Duox1, Epx, Lpo, Mpo, Ptgs2, Rag2, Serpinb1b, Tmod1 and Tpo. The genes that up regulated in the reactive oxygen species cluster (16 genes): Il19, Il22, Nos2, Nox1, Noxa1, Noxo1, Recql4 and Sod2. The genes that were up regulated in the oxidative stress cluster (22 genes) were: Mpp4, Nudt15, Upc3 and Xpa. The genes that were up regulated in the oxygen carriers cluster (12 genes) were: Hbq1, Mb, Ngb, Slc38a1 and Xirp1. The peroxiredoxins genes (10) showed no consistent differential regulation. CONCLUSION: The genes related to oxidative stress and antioxidant defense showed increased expression in renal tissue trigged intestinal ischemia and reperfusion.
- ItemAcesso aberto (Open Access)Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-03-01) Ikejiri, Adauto Tsutomu; Somaio Neto, Frederico; Chaves, Jose Carlos; Bertoletto, Paulo Roberto; Teruya, Roberto; Bertoletto, Eduardo Rodrigues; Taha, Murched Omar [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Federal University of Grande Dourados Medical School; UFGD Medical School; Federal University of Mato Grosso do Sul Surgery Department; Universitary Center Sao Camilo; Universidade Federal de São Paulo (UNIFESP)PURPOSE:To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS:Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2
- ItemAcesso aberto (Open Access)Heparin modulates the expression of genes encoding pro and anti-apoptotic proteins in endothelial cells exposed to intestinal ischemia and reperfusion in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-07-01) Taha, Murched Omar [UNIFESP]; Miranda-Ferreira, Regiane [UNIFESP]; Taha, Nabiha Saadi Abrahão [UNIFESP]; Monteiro, Hugo Pequeno [UNIFESP]; Caricati-Neto, Afonso [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate if expression of genes encoding pro and anti-apoptotic proteins in the rat enteric endothelial cells stimulated by intestinal ischemia followed by reperfusion (IR) can be modified by treatment with heparin (HP).METHODS: Eighteen adult Wistar rats were divided in three groups: sham group submitted to laparotomy only (SG), ischemia followed by reperfusion group (IRG); ischemia followed by reperfusion plus pretreatment with HP 100 mg.kg-1 (IRG+HP). Ischemia was performed by clamping of the superior mesenteric artery. After 60 min of ischemia, metal clamps were removed for reperfusion for 120 min. Gene expression of encoding pro (Casp1, Casp6, Casp3, Cflar, Fas and Pgl) and anti-apoptotic (Bcl2, Bcl2l1 and Naip2) proteins in rat enteric endothelial cells was evaluated by PCR microarray method.RESULTS: Compared to rat endothelial cells of SG, the expression of pro-apoptotic genes was up-regulated in IRG while anti-apoptotic genes were down-regulated. In contrast, the expression of anti-apoptotic genes in IRG+HP was up-regulated while pro-apoptotic genes was down-regulated compared to SG.CONCLUSION: The attenuation by heparin of intestinal ischemia-reperfusion previously demonstrated in rodents could be related with ability of this drug to stimulate and reduce gene expression of encoding anti and pro-apoptotic proteins, respectively.
- ItemAcesso aberto (Open Access)Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion(Acta Cirurgica Brasileira, 2017) Somaio Neto, Frederico; Ikejir, Adauto Tsutomo; Bertoletto, Paulo Roberto; Chaves, Jose Carlos; Teruya, Roberto; Fagundes, Djalma Jose [UNIFESP]Purpose: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Methods: Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, which received HBO during ischemia; HBO-RG, which received HBO during reperfusion; and HBOIRG, which received HBO during ischemia and reperfusion. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes. The (RT-qPCR) method was applied. Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student's t-test p< 0.05). Results: Eight genes (9.52%) were hyperexpressed in the IRG. When the HBO groups were compared to the IRG, we found a decrease in the expression of eight genes in the HBO-IG, five genes in the HBO-RG, and seven genes in the HBO-IRG. Conclusion: The reduction in the expression of genes related to oxidative stress and antioxidant defense following HBO in mouse hearts resulting from intestinal IR injury was more favorable during the ischemic period than during the reperfusion period.
- ItemAcesso aberto (Open Access)Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-03-01) Taha, Murched Omar [UNIFESP]; Miranda-Ferreira, Regiane [UNIFESP]; Taha, Nabiha Saadi Abrahão [UNIFESP]; Monteiro, Hugo Pequeno [UNIFESP]; Caricati-Neto, Afonso [UNIFESP]; Oliveira-Júnior, Itamar Souza [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R). METHODS: Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology. RESULTS: Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC. CONCLUSION: Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.
- ItemAcesso aberto (Open Access)Pretreatment with pentoxifylline attenuates lung injury induced by intestinal ischemia/reperfusion in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-12-01) Marqui, Carlos Eduardo [UNIFESP]; Silva, Helga Cristina Almeida da [UNIFESP]; Ferez, David [UNIFESP]; Cavassani, Sâmia Santos [UNIFESP]; Moraes, Juliana Britto [UNIFESP]; Silva, Danielle Aparecida Marino da [UNIFESP]; Simões, Ricardo Santos [UNIFESP]; Lopes, Caroline Aparecida [UNIFESP]; Taha, Murched Omar [UNIFESP]; Oliveira-Júnior, Itamar Souza [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate the protective effect of pentoxifylline against the lung injury observed after intestinal ischemia (I) followed by a period of reperfusion (R). METHODS: Twenty-eight male Wistar rats were equally divided into 4 experimental groups and operated under ketamine-xylazine anesthesia. (1) Sham: falsely-operated animals; (2) SS+IR: intestinal ischemia was accomplished by clipping the superior mesenteric artery during 60 minutes, with an administration of a standard volume of saline solution (SS) 5 min before the end of the ischemia period; the clip was then releases or a 120-min period of reperfusion; (3) I+PTX+R: ischemia as above, PTX was administered (25 mg/kg) and the gut reperfused as above; (4) PTX+I+PTX+R: Five minutes before arterial occlusion PTX was administered; the superior mesenteric artery was then clipped for 60 minutes. After 55-min ischemia, an additional dosis of PTX was administered; the clip was removed for reperfusion as above. At the 60th min of reperfusion a third dosis of PTX was administered. RESULTS: PTX markedly attenuated lung injury as manifested by significant decreases (all P<0.001 as compared with the SS+IR group) of pulmonary wet/dry tissue weight ratio, total protein content, myeloperoxidase activity and tumor necrosis factor-alpha. Moreover, it was apparent that in the group PTX+I+PTX+R the improvements have been even more significant. CONCLUSION: PTX exerted a protective effect on the lung from the injuries caused by intestinal ischemia/reperfusion.
- ItemAcesso aberto (Open Access)The role of ischemic preconditioning and pentoxifylline in intestinal ischemia/reperfusion injury of rats(Acta Cirurgica Brasileira, 2017) Ribeiro de Oliveira, Teresinha Regina [UNIFESP]; de Oliveira, Geraldo Ferreira; Simoes, Ricardo Santos [UNIFESP]; Tikazawa, Eduardo Hiroshi; Monteiro, Hugo Pequeno [UNIFESP]; Fagundes, Djalma Jose [UNIFESP]; Taha, Murched Omar [UNIFESP]Purpose: To investigate the role of ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal mucosa ischemia/reperfusion injury (IR). Methods: Thirty rats were assigned to 5 groups (N= 6): (CG): no clamping of the superior mesenteric artery (90 min.)
- ItemAcesso aberto (Open Access)The role of ischemic preconditioning at the gracilis muscle of rats in the early phase of reperfusion injury(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2006-04-01) Webster, Ronaldo Scholze [UNIFESP]; Montero, Edna Frasson de Souza [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Zettler, Cláudio Galleano; Coiro, José; Universidade Federal de São Paulo (UNIFESP); Federal College Foundation of Porto Alegre; Lutheran University of Brazil Electronic Microscopy LaboratoryPURPOSE: Verify the role of ischemic preconditioning (IPC) in ischemia and reperfusion injury on gracilis muscle of rats. METHODS: Wistar rats (n=30) were distributed in three groups, I/R and IPC groups were subdivided concerning ischemia time. A near-amputation model of the posterior limb was produced by a hip joint level incision, preserving the vascular bundle and the femur bone and ischemia was induced for 2h and 4h, G-I 2h/R (n=6) and G-I 4h/R (n=6), followed by 1h of vascular reperfusion. The preconditioned groups, G-PCI 2h (n=6) and G-PCI 4h (n=6), were preceded by 3 cycles of 5min of ischemia followed by 5min of vascular reperfusion before sustained ischemia. In the Control Group, C-G (n=6) animals were subjected to regional approach. The analysis was done with Light Microscopy (LM). RESULTS: The levels of fibril fragmentation were progressive in the G-I 2h/R (67% of muscle preservation) and in the G-I4 h/R (0% of muscle preservation). However in the group of the precondition the lesion degree being in level similar to the group controls in the G-I 2h/R (100% of muscle preservation) while at G-I 4h/r occur less protection (67% of muscle preservation). The degree of tissue inflammatory reaction was worst at G-I 4h/R (0% without inflammation signals) than at G-I 2h/R (50% without inflammation signals); while in the precondition group G-IPC-2h (83% without inflammation signals) was better than the G-IPC-4h (67% without inflammation signals). The vascular stasis was absent only in 17% of the G-I 4h/R and in 33% of the G-I 2h/R. In precondition group, however, the vascular stasis was absent in 33% at G-IPC 2h and absent in 50% at G-IPC 4h. CONCLUSION: The IPC showed, in an earlier phase, a benefic role at I/R derived injury on gracilis muscle of rats, as proven for the largest preservation of the fibers muscular, smaller inflammatory reaction and smaller vascular stasis.
- ItemAcesso aberto (Open Access)Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-04-01) Armstrong, Dinani Matoso Fialho de Oliveira; Armstrong, Anderson da Costa; Figueiredo, Regina Célia Bressan Queiroz; Florentino, Joao Eduardo; Saad, Paulo Fernandes [UNIFESP]; Fox-Talbot, Karen; Halushka, Marc Kenneth; Berkowitz, Dan E.; Taha, Murched Omar [UNIFESP]; Fagundes, Djalma José [UNIFESP]; Sao Francisco Valley Federal University Department of Surgery; UNIVASF Department of Cardiology; Research Center Aggeu Magalhaes Department of Microbiology; Pernambuco Federal University; UNIVASF Department of Surgery; Johns Hopkins University Department of Pathology; Johns Hopkins University Department of Anesthesiology and Critical Care Medicine; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.
- ItemAcesso aberto (Open Access)Very short cycles of postconditioning have no protective effect against reperfusion injury. Experimental study in rats(Sociedade Brasileira de Cirurgia Cardiovascular, 2014-12-01) Nakamura, Ricardo Kenithi; Santos, Carlos Henrique Marques Dos; Miiji, Luciana Nakao Odashiro [UNIFESP]; Arakaki, Mariana Sousa; Maedo, Cristiane Midori; Érnica Filho, Maurício; Cassino, Pedro Carvalho; Pontes, Elenir Rose Jardim Cury; Universidade Federal de Mato Grosso do Sul Faculdade de Medicina; Universidade Federal de São Paulo (UNIFESP)Introduction: Ischemic postconditioning has been recognized as effective in the prevention of reperfusion injury in situations of ischemia and reperfusion in various organs and tissues. However, it remains unclear what would be the best way to accomplish it, since studies show great variation in the method of their application. Objective: To assess the protective effect of ischemic postconditioning on ischemia and reperfusion in rats undergoing five alternating cycles of reperfusion and ischemia of 30 seconds each one. Methods: We studied 25 Wistar rats distributed in three groups: group A (10 rats), which underwent mesenteric ischemia (30 minutes) and reperfusion (60 minutes); Group B (10 rats), undergoing ischemia (30 minutes) and reperfusion (60 minutes), intercalated by postconditioning (5 alternating cycles of reperfusion and ischemia of 30 seconds each one); and group C - SHAM (5 rats), undergoing only laparotomy and manipulation of mesenteric artery. All animals underwent resection of an ileum segment for histological analysis. Results: The mean lesions degree according to Chiu et al. were: group A, 2.77, group B, 2.67 and group C, 0.12. There was no difference between groups A and B (P>0.05). Conclusion: Ischemic postconditioning was not able to minimize or prevent the intestinal tissue injury in rats undergoing ischemia and reperfusion process when used five cycles lasting 30 seconds each one.