Navegando por Palavras-chave "Rectus Abdominis"
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- ItemAcesso aberto (Open Access)Cadaver as an experimental a model for the study of midline incisional hernia(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-08-01) Silveira, Romar Ângelo Barbato [UNIFESP]; Nahas, Fabio Xerfan [UNIFESP]; Hochman, Bernardo [UNIFESP]; Bazzano, Felix Carlos Ocariz; Amorim, Carlos Roberto [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); UNIVAS Bioethical Postgraduate ProgramPURPOSE: To describe a cadaver model and to compare different techniques to correct midline incisional hernia using the tension at the musculoaponeurotic layer as a parameter. METHODS: Twenty-four male cadavers were used to characterize the model. The tensile force applied to the anterior layers of the rectus abdominis muscle was measured after the linea alba was resected to create an abdominal wall defect. A dynamometer was used to measure the tensile force necessary to cause a 10-mm displacement of the tissues towards the midline. An index that allows comparison of tensile forces from different points was calculated by dividing the tensile force by the distance between the point of force application at the aponeurosis and the midline. RESULTS: Cadaver is a good model for the study of midline incisional hernia. CONCLUSION: Cadaver can be used effectively as a model to evaluate tension of the abdominal wall when tissue advancement and musculoaponeurotic flaps are performed in the repair of midline incisional hernia.
- ItemAcesso aberto (Open Access)An experimental model for the study of collagen fibers in skeletal muscle(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-10-01) Calvi, Eliziane Nitz de Carvalho [UNIFESP]; Nahas, Fabio Xerfan [UNIFESP]; Barbosa, Marcus Vinicius [UNIFESP]; Calil, José Augusto; Ihara, Silvia Saiuli Miki [UNIFESP]; Silva, Marcelo de Souza; Franco, Marcello Fabiano de [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.
- ItemAcesso aberto (Open Access)Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2011-01-01) Calvi, Eliziane Nitz de Carvalho [UNIFESP]; Nahas, Fabio Xerfan [UNIFESP]; Barbosa, Marcus Vinicius [UNIFESP]; Ihara, Silvia Saiuli Miki [UNIFESP]; Calil, José Augusto; Ferreira, Lydia Masako [UNIFESP]; UNINOVE Department of Physical Therapy; Universidade Federal de São Paulo (UNIFESP)PURPOSE: To assess the collagen content and types in the rectus abdominis muscle of cadavers of different ages. METHODS: Forty fresh adult male cadavers, at room temperature, were obtained from the Institute of Legal Medicine of Franca and dissected within 24 hours of death. The cadavers were divided into two groups: Group A (n=20), 18 to 30 years of age, and Group B (n=20), 31 to 60 years of age. Bilateral incisions were made in the middle portion of anterior rectus sheath 3 cm superiorly and 2 cm inferiorly to the umbilicus and four fragments of the rectus abdominis muscle were dissected. The samples were fixed in 10% buffered formalin and sent for immunohistochemical analysis to determine collagen content and types. RESULTS: Immunohistochemical results revealed higher amounts of type I and type III collagen in Group A. However, no difference in the amount of type IV collagen was found between the groups. CONCLUSION: The amount of type I and type III collagen was higher in group A.
- ItemAcesso aberto (Open Access)Nicotine on rat TRAM flap(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2009-06-01) Ely, Pedro Bins; Kobayashi, Ludmila Aimi [UNIFESP]; Campos, José Humberto Oliveira; Gomes, Heitor Carvalho [UNIFESP]; Juliano, Yara [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Unit Santa Casa de Porto Alegre Plastic Surgery; Universidade Federal de São Paulo (UNIFESP); Escola Bahiana de Medicina Department of Surgery Head of Operative Technique and Experimental Surgery DivisionPURPOSE: The transverse rectus abdominis myocutaneous (TRAM) flap is one of the preferential techniques used in breast reconstruction following mastectomy. Nicotine has a detrimental effect on cutaneous flap survival; although there are no experimental studies proving this effect on musculocutaneous flaps. The aim of this study is to verify the effect of nicotine on the rat TRAM flap. METHODS: 30 Wistar EPM-1 rats were randomly distributed in two groups: control and experimental. The animals of the control group received saline solution injected subcutaneously, in a volume of 0.2 ml, twice a day, during 28 days in the preoperative period. The animals of the experimental group were treated with nicotine, injected subcutaneously, in a dose of 2 mg/kg twice a day, during 28 days in the preoperative period. All the animals were submitted to the caudally based, right unipedicled TRAM flap. 48 hours after the procedure, a study of the viable area of the flap was done through photographic documentation. Statistical analysis was performed with nonparametric Mann-Whitney's test. RESULTS: The experimental group had significantly greater area of necrosis when compared with the control group (p<0.001). CONCLUSION: Nicotine increased the area of necrosis of the TRAM flap, in rats.
- ItemAcesso aberto (Open Access)Ultrasonography for measuring rectus abdominis muscles diastasis(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2007-06-01) Mendes, Denise de Almeida [UNIFESP]; Nahas, Fabio Xerfan [UNIFESP]; Veiga, Daniela Francescato; Mendes, Fernando Vilela; Figueiras, Ricardo Góes; Gomes, Heitor Carvalho [UNIFESP]; Ely, Pedro Bins; Novo, Neil Ferreira [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); UNIVAS; UNIVAS Surgery Department Plastic Surgery Division; Minas Center of Mastology and Imaging; UNIVAS Surgery Department Plastic Division; Santa Casa Plastic Surgery UnitPURPOSE: Preoperative imaging evaluation may be useful for determining the position of recti abdominis muscles before their correction. The purpose of this study is to evaluate the accuracy of ultrasonography to measure the width of rectus abdominis muscle diastasis. METHODS: Rectus diastasis was measured by ultrasonography preoperatively in 20 females. Rectus diastasis was measured in seven levels along the anterior rectus sheath by ultrasound after sustained deep inspiration and after expiration. Rectus diastasis, at the same levels, was also measured after its exposition during abdominoplasty by two independent observers, using a surgical compass. These values were compared using Wilcoxon's statistical text, for non independent values. RESULTS: There was no significant difference between the values obtained by ultrasound and those measured during surgery in the supra-umbilical levels and at the level of the umbilicus. However, below the umbilicus these values differed significantly, showing smaller values in the imaging evaluation. CONCLUSION: Ultrasonography is an accurate method to measure rectus diastasis above the umbilicus and at the umbilical level.