Navegando por Palavras-chave "Plectranthus barbatus"
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- ItemAcesso aberto (Open Access)Anti-trypanosomal activity of 1,2,3,4,6-penta-O-galloyl-β -D-glucose isolated from Plectranthus barbatus Andrews (Lamiaceae)(Sociedade Brasileira de Química, 2012-01-01) Santos, Roberta Tancredi dos [UNIFESP]; Hiramoto, Liliane Lumi [UNIFESP]; Lago, Joao Henrique Ghilardi [UNIFESP]; Sartorelli, Patricia [UNIFESP]; Tempone, André Gustavo [UNIFESP]; Pinto, Erika Gracielle; Universidade Federal de São Paulo (UNIFESP); Instituto Adolfo Lutz Departamento de Parasitologia; Universidade de São Paulo (USP); Instituto Plantarum de Estudos da FloraMeOH extract from the leaves of Plectranthus barbatus Andrews (Lamiaceae), showed in vitro anti-trypanosomal activity. The bioassay-guided fractionation resulted in the isolation of a gallic acid derivative, identified as 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG), after thorough NMR and MS spectral analysis. Finally, this compound was tested against trypomastigote forms of T. cruzi and displayed an EC50 value of 67 µM, at least 6.6-fold more effective than the standard drug benznidazole. This is the first occurrence of PGG in the Plectranthus genus and the first anti-parasitic activity described for PGG in the literature.
- ItemAcesso aberto (Open Access)Avaliação da atividade biológica de metabólitos de Lippia alba (Verbenaceae), Plectranthus amboinicus e Plectranthus barbatus (Lamiaceae)(Universidade Federal de São Paulo, 2016-02-19) Santos, Nara Oshiro dos [UNIFESP]; Sartorelli, Patricia [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)This study aimed to identify substances with antimicrobial, antiparasitic and antitumor activities of extract and essential oil from the leaves of Lippia alba (Mill.) N.E. Brown and evaluate the antimicrobial and antitumor activities of essential oils from leaves of Plectranthus amboinicus (Lour.) Spreng and Plectranthus barbatus Andrews. The major compounds identified in the essential oil of L. alba leaves were monoterpenes nerol/geraniol (27%) and neral/geranial (22%), the ketone, 6-methyl-5- hepten-2-one (12%) and sesquiterpene, (E)-caryophyllene (9%). This oil showed cytotoxic activity in vitro against B16F10 (murine melanoma) with IC50 of 45.82 µg/mL. Furthermore, it was found that the oil has antimicrobial activity in vitro, inhibiting Gram-negative bacteria, except Pseudomonas aeruginosa, Gram-positive bacteria and yeasts. The dichloromethane phase obtained from the methanol extract of the leaves of this species showed activity against Leishmania (Leishmania) amazonensis with IC50 of 50.8 µg/mL and among the active groups of fractions were identified triterpenes mixture of oleanolic and ursolic acids, in addition to the steroid stigmasterol, all described in L. alba for the first time. The essential oil from leaves of P. amboinicus presented as major compounds carvacrol (38%), γ-terpinene (15%), (Z)-caryophyllene (14%) and p-cymene (12%), showing cytotoxic activity against B16F10 with IC50 similar to standard drug, however, twice more specific, in addition, showed antimicrobial activity against S. epidermidis, C. albicans, C. neoformans (serotype D), C. gattii (serotypes B and C) and S. cerevisiae. In turn, essential oil from leaves of P. barbatus presentes as major compounds (Z) -caryophyllene (18%), germacrene -D (17%), viridiflorene (14%), cyclosativene (10%) and α -pinene (9%), and has demonstrated activity against C. neoformans (serotype A) and C. gattii (serotype C).
- ItemSomente MetadadadosInhibition of the gastric H+,K+-ATPase by plectrinone A, a diterpenoid isolated from Plectranthus barbatus Andrews(Elsevier B.V., 2007-04-20) Schultz, Carla; Bossolani, Myllene P.; Torres, Luce M. B.; Lima-Landman, Maria Teresa R.; Lapa, Antonio J.; Souccar, Caden; Universidade Federal de São Paulo (UNIFESP); Bot Garden São PauloThis work assessed the mechanism underlying the antisecretory gastric acid effect of Plectranthus barbatus Andrews (Lamiaceae) and active constituents. Popularly known as false-boldo, this plant is used in Brazilian folk medicine to treat gastrointestinal and hepatic ailments. the plant aqueous extract (AE) and isolated compounds were assayed in vivo in pylorus-ligated mice, and in vitro on acid secretion measured as [C-14] -arninopyrine ([C-14]-AP) accumulation in rabbit gastric glands and gastric H+,K+-ATPase preparations. Injected into the duodenal lumen, the AE of the plant leaves (0.5 and 1.0g/kg) decreased the volume (62 and 76%) and total acidity (23 and 50%) of gastric acid secretion in pylorus-ligated mice. Bioguided purification of the AE yielded an active fraction (IC50 = 24 kg/ml) that inhibited acid secretion in rabbit gastric glands with a potency 10 to 18 times greater than that of the originating extract, on both the basal and stimulated acid secretion by histamine (His) (1 mu M) or bethanechol (100 mu M). At the same concentrations the gastric H+,K+-ATPase activity was also inhibited. the active constituent was chemically identified as the abietanoid dienedione plectrinone A which reduced the H+,(+)-ATPase activity with IC50 = 171 mu M. the results indicate that inhibition of the gastric proton pump by this diterpenoid may account for the antisecretory acid effect and reputed antiulcer activity of Plectranthus barbatus. (c) 2006 Elsevier Ireland Ltd. All rights reserved.