Navegando por Palavras-chave "Phagocytosis"
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- ItemSomente MetadadadosAtividade fagocÍtica de monócitos em pacientes com carcinoma, antes e após imunoterapia com glucana(Universidade Federal de São Paulo (UNIFESP), 1984) Taketomi, Ernesto Akio [UNIFESP]; Mendes, Nelson Figueiredo [UNIFESP]
- ItemAcesso aberto (Open Access)Avaliação da atividade fagocítica e microbicida de macrófagos alveolares de ratos de baixo peso ao nascer(Universidade Federal de São Paulo, 2023-01-12) Negreiros, Náthani Gabrielly Silva [UNIFESP]; Landgraf, Richardt Gama [UNIFESP]; Azevedo, Gabriela Araújo de; http://lattes.cnpq.br/2102197172721190; http://lattes.cnpq.br/9656990228494629; http://lattes.cnpq.br/6155381223665537A restrição alimentar materna durante a gravidez induz adaptações fetais morfológicas e metabólicas e predispõe a doenças metabólicas, incluindo diabetes e hipertensão, na prole. Distúrbios metabólicos também podem ser induzidos pelo desequilíbrio de fatores pró e anti-inflamatórios, como observado na sepse. Em estudos anteriores, nosso grupo demonstrou que a restrição alimentar durante a gestação resultou em baixo peso ao nascer, hipocelularidade na medula óssea e no sangue periférico, redução na migração de leucócitos e baixa resposta inflamatória em ratas Wistar com 12 semanas. O objetivo deste estudo foi avaliar a capacidade fagocítica e microbicida de macrófagos alveolares, de animais que sofreram restrição alimentar intrauterina. Para isso, os animais foram acasalados, ratos Wistar fêmeas e machos da mesma idade (12 a 16 semanas de idade). O dia 1 da gestação foi definido como o dia em que os espermatozoides foram detectados no esfregaço vaginal. Após a confirmação da ocorrência do acasalamento, as ratas foram divididas aleatoriamente em 2 grupos: Controles - dieta e água ad libitum; Restritos - 50% de restrição alimentar e água ad libitum. Com 12 semanas de idade, ratos machos descendentes de ambos os grupos foram eutanasiados e os macrófagos alveolares incubados com Saccharomyces cerevisiae, na proporção de 4:1, por 4 horas. Para avaliar a atividade microbicida foi feita a contagem das unidades formadoras de colônia (UFC) dessas células com Saccharomyces cerevisiae. Observamos que as mães que sofreram restrição alimentar não apresentaram ganho de peso durante o período gestacional e os filhotes nasceram com baixo peso e tamanho reduzido. Após o 20º dia de vida, os filhotes de baixo peso (restrito) igualaram-se ao peso dos filhotes de peso normal (controle), superando a diferença de massa corporal entre os grupos. O grupo restrito apresentou menor atividade fagocítica e microbicida quando comparado ao grupo controle. Os resultados obtidos até o momento indicam que a menor capacidade fagocitária e microbicida dos macrófagos de ratos de baixo peso ao nascer pode estar contribuindo para o desenvolvimento de doenças.
- ItemAcesso aberto (Open Access)Estrutura do rim cefálico, hematologia e atividade fagocítica de macrófago do robalo Centropomus parallelus de cativeiro e de vida livre(Universidade Federal de São Paulo (UNIFESP), 2009-06-24) Santos, Antenor Aguiar [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The objective of this work was to determine hematological parameters and the phagocytic capacity of peritoneal macrophages of the fat snook related to sex, stage of gonadal maturation and seasonal cycle. The hematological results did not show significant differences between males and females. With respect to stage of gonadal maturation, only erythrocytes numbers (RBC), in females, was found to be significantly elevated in stage III (maturing) and decreased in V (spent). An analysis of the results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to sex and seasonal cycle showed statistically significant differences: a) in males, hematocrit (Ht) and hemoglobin concentration (Hb) were elevated in the spring and low in the winter; b) in both sexes, mean corpuscular volume (MCV) was high in the spring and summer and low in the fall; c) in females, thrombocytes numbers were elevated in the fall and low in the other seasons; d) in both sexes, phagocytic capacity (PC) and phagocytic index (PI) were higher in the summer and lower in the fall. The results showed that spring and summer correspond to seasons of the year for better hematological and phagocytic responses for survival of the fat snook in its natural habitat. The parameters studied could be utilized for evaluation of the health status in its own habitat or in captivity.
- ItemSomente MetadadadosHematological parameters and phagocytic activity in fat snook (Centropomus parallelus) bred in captivity(Elsevier B.V., 2012-10-01) Santos, Antenor Aguiar; Ranzani-Paiva, Maria Jose T.; Veiga, Marcelo Leite da; Faustino, Lucas; Egami, Mizue Imoto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Adventist Univ; Fishery Inst; Universidade Federal de Sergipe (UFS); Universidade de São Paulo (USP)The objective of this work was to determine the hematological parameters and the phagocytic capacity of peritoneal macrophages of fat snook related to sex, stage of gonadal maturation and seasonal cycle. Blood was collected from 135 animals (78 females and 57 males) and used for determinations of: erythrocyte number, hematocrit, hemoglobin, erythrocyte indices mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), total and differential leukocyte counts, and thrombocyte count. the phagocytic capacity and phagocytic index were determined after Saccharomyces cerevisiae inoculation in the peritoneal cavity of the animals. the hematological results according to sex showed that the erythrocyte, total leukocyte and thrombocyte counts were statistically higher in males than females, with the latter showing a higher MCV. Concerning to erythrocyte count, hematocrit and hemoglobin concentration analyzed separately by sex and stage of gonadal maturation, males were found to have significantly elevated values in the mature stage and decreased levels in the resting stage. the results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to seasonal cycle showed significant differences in both sexes, where hematocrit and hemoglobin concentration were lower in winter and higher in the other seasons, mean corpuscular volume was higher in the summer and lower in the winter and fall, total leukocytes and thrombocytes lower in the spring and higher in the fall, lymphocytes low in the winter and summer and high in the spring and phagocytic capacity and phagocytic index high in the summer and low in the winter and fall. the results showed that the hematological values in males are statistically higher than those in females, the erythrocyte values in males increase with the progression of gonadal maturation and that winter is the season of the year least favorable for hematological and phagocytic responses for survival of fat snook kept in captivity. the parameters studied could be utilized in the evaluation of the health status of this species in captivity. (C) 2012 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosHematological parameters and phagocytic activity in fat snook (Centropomus parallelus): Seasonal variation, sex and gonadal maturation(Elsevier B.V., 2009-11-16) Santos, Antenor Aguiar [UNIFESP]; Egami, Mizue Imoto [UNIFESP]; Ranzani-Paiva, Maria Jose T.; Juliano, Yara; Adventist Univ Ctr São Paulo UNASP; Universidade Federal de São Paulo (UNIFESP); Fishery Inst; Univ Santo AmaroThe objective of this work was to determine hematological parameters and the phagocytic capacity of peritoneal macrophages of the fat snook related to sex. stage of gonadal maturation and seasonal cycle. the hematological results did not show significant differences between males and females. With respect to stage of gonadal maturation, only erythrocytes numbers (RBC), in females, was found to be significantly elevated in stage III (maturing) and decreased in V (spent). An analysis of the results of the erythrocyte and leukocyte series, thrombocytes and phagocytic activity related to sex and seasonal cycle showed statistically significant differences: a) hematocrit (Ht) and hemoglobin concentration (Hb) wet e elevated in the spring and low in the winter in males; b) mean corpuscular volume (MCV) was high in the spring and summer and low in the fall in both sexes; c) thrombocytes numbers were elevated in the fall and low in the other seasons in females: d) phagocytic capacity (PC) and phagocytic index (PI) were higher in the summer and lower in the fill in females. the results showed that spring and summer correspond to seasons of the year for better hematological and phagocytic responses for survival of the fat snook in its natural habitat. the parameters studied could be utilized for evaluation of the health status fat snook in its own habitat or in captivity. (C) 2009 Elsevier B.V. All rights reserved.
- ItemAcesso aberto (Open Access)Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species(Associação Brasileira de Divulgação Científica, 2010-09-01) Fernandes, Maria da Luz [UNIFESP]; Mendes, Marialice Erdelyi [UNIFESP]; Brunialti, Milena Karina Coló [UNIFESP]; Salomão, Reinaldo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to increasing LPS concentrations resulted in lower intracellular concentration of IL-6 in monocytes after challenge. A similar effect was observed with challenge with MALP-2 (a Toll-like receptor (TLR)2/6 agonist) and killed Pseudomonas aeruginosa and S. aureus, but not with flagellin (a TLR5 agonist). LPS conditioning with 15 ng/mL resulted in a 40% reduction of IL-6 in monocytes. In contrast, phagocytosis of P. aeruginosa and S. aureus and induced ROS generation were preserved or increased in tolerant cells. The phenomenon of tolerance involves a complex regulation in which the production of IL-6 was diminished, whereas the bacterial phagocytosis and production of ROS was preserved. Decreased production of proinflammatory cytokines and preserved or increased production of ROS may be an adaptation to control the deleterious effects of inflammation while preserving antimicrobial activity.
- ItemSomente MetadadadosInvolvement of proteinase-activated receptors 1 and 2 in spreading and phagocytosis by murine adherent peritoneal cells: Modulation by the C-terminal of S100A9 protein(Elsevier B.V., 2010-02-25) Pagano, Rosana L.; Sampaio, Sandra C.; Juliano, Maria A. [UNIFESP]; Juliano, Luiz [UNIFESP]; Giorgi, Renata; Butantan Inst; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Proteinase-activated receptors (PAR) are widely recognized for their modulatory properties in inflammatory and immune responses; however, their direct role on phagocyte effector functions remains unknown. S100A9, a protein secreted during inflammatory responses, deactivates activated peritoneal macrophages, and its C-terminal portion inhibits spreading and phagocytosis of adherent peritoneal cells. Herein, the effect of PAR1 and PAR2 agonists was investigated on spreading and phagocytosis by adherent peritoneal cells, as well as the ability of murine C-terminal of S100A9 peptide (mS100A9p) to modulate this effect. Adherent peritoneal cells obtained from mouse abdominal cavity were incubated with PAR1 and PAR2 agonists and spreading and phagocytosis of Candida albicans particles were evaluated. PAR1 agonists increased both the spreading and the phagocytic activity, but PAR2 agonists only increased the spreading index. mS100A9p reverted both the increased spreading and phagocytosis induced by PAR1 agonists, but no interference in the increased spreading induced by PAR2 agonists was noticed. the shorter homologue peptide to the C-terminal of mS100A9p, corresponding to the H(92)-E(97) region, also reverted the increased spreading and phagocytosis induced by PAR1 agonists. These findings show that proteinase-activated receptors have an important role for spreading and phagocytosis of adherent peritoneal cells, and that the pepticle corresponding to the C-terminal of S100A9 protein is a remarkable candidate for use as a novel compound to modulate PAR1 function. (C) 2009 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosMicrobicidal property of B1 cell derived mononuclear phagocyte(Elsevier B.V., 2009-08-01) Popi, A. F. [UNIFESP]; Zamboni, D. S.; Mortara, R. A. [UNIFESP]; Mariano, M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)A mononuclear phagocyte derived from B1b cells (B1CDP) has been described. As these cells migrate from the peritoneal cavity to non-specific inflammatory lesion sites and are highly phagocytic via Fc and mannose receptors, their microbicidal ability of these cells was investigated using the Coxiella burnetii cell infection model in vitro. in this report, the pattern of infection and C burnetii phase II survival in B1CDP phagosomes was compared with the pattern of infection of peritoneal macrophages from Xid mice (PM phi) and bone marrow derived macrophages (BMM phi). Infection was assessed by determining the large parasitophorous vacuole formation, the relative focus forming units and the quantification of DAPI (4',6-diamino-2-phenylindole) fluorescence images acquired by confocal microscopy. When compared to macrophages, B1CDP are more permissive to the bacterial infection and less effective to kill them. Further, results suggest that IL-10 secreted by B1 cells are involved in their susceptibility to infection by C burnetti, since B1CDP from IL-10 KO mice are more competent to control C. burnetii infection than cells from wild type mice. These data contribute further to characterize B1CDP as a novel mononuclear phagocyte. (C) 2008 Elsevier GmbH. All rights reserved.
- ItemAcesso aberto (Open Access)Modulação multifacetada da função dos monócitos na sepse : comprometimento na apresentação de antígeno, aumento na diferenciação não clássica e na expressão do receptor de fagocitose(Universidade Federal de São Paulo (UNIFESP), 2017-09-20) Mota, Nadijane Valeria Ferreira da [UNIFESP]; Salomão, Reinaldo [UNIFESP]; http://lattes.cnpq.br/8370334857007434; http://lattes.cnpq.br/9218049003813361; Universidade Federal de São Paulo (UNIFESP)RESUMO Monócitos e macrófagos são fundamentais na resposta do hospedeiro à sepse, reconhecendo o microrganismo infectante e desencadeando uma resposta inflamatória. Essas funções são, pelo menos em parte, moduladas pela expressão de receptores de superfície celular. Pretendemos caracterizar o fenótipo de monócitos de pacientes sépticos durante o curso da sepse e sua associação com os desfechos clínicos. Sessenta e um pacientes sépticos e 31 voluntários sadios foram incluídos no estudo. As amostras foram obtidas de pacientes na admissão (D0, N = 61) e após 7 (D7, N = 36) e 14 dias de terapia (D14, N = 22). Os monócitos de pacientes sépticos apresentaram expressão diminuída de CD86, HLA-DR, CD200R, CCR2, CXCR2 e CD163 em comparação com monócitos de voluntários sadios. Em contraste, a expressão de PD-1, PD-L1, CD206, CD64 e CD16 estava aumentada nos pacientes. A expressão de HLA-DR, CXCR2, CD64, PD-1 e PD-L1 foi maior nos sobreviventes do que nos não sobreviventes. Houve aumento de expressão de CD86, HLA-DR e CXCR2 em amostras de seguimento; em contraste, CD64 e CD16 GMFI diminuíram ao longo do tempo. Em conclusão, os monócitos de pacientes sépticos apresentam comprometimento da apresentação de antígenos caracterizados por diminuição da expressão de HLA-DR e do coestimulante CD86 e aumento da expressão de PD-1 e PD-L1. Por outro lado, o aumento das atividades inflamatórias e fagocíticas de monócitos pode ser inferido pelo aumento da expressão de CD16 e CD64. Encontramos resultados conflitantes em relação à diferenciação em relação ao fenótipo M2, com aumento da expressão de CD206 e diminuição da expressão de CD163 em monócitos de pacientes sépticos, enquanto o subconjunto de monócitos não clássicos foi demonstrado pelo aumento do CD16hi. HLA-DR, CD64, PD-1 e PD-L1 foram mais expressos nos sobreviventes do que nos não sobreviventes. Palavras-chave: M1 e M2, monócitos clássicos e não-clássicos, fagocitose, imunomodulação, resposta inflamatória.
- ItemAcesso aberto (Open Access)Peptideos derivados da gp43 de Paracoccidioides brasiliensis inibem a fagocitose(Universidade Federal de São Paulo (UNIFESP), 2008) Konno, Adriana Yumi de Camargo [UNIFESP]; Lopes, Jose Daniel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Paracoccidioidomicose (PCM) é uma doença granulomatosa sistêmica causada pelo fungo dimórfico térmico Paracoccidioides brasiliensis. O principal antígeno desse fungo é uma glicoproteína de 43-KDa. Gp43 possui diferentes funções: participa do mecanismo de evasão durante a instalação da infecção primária e estimula formação de granuloma in vitro, apresentando epítopos reconhecidos por células T que induzem resposta protetora contra o P. brasiliensis. Aqui, investigamos quais epítopos da gp43 poderiam diminuir a função fagocítica de macrófagos e a reação inflamatória correspondente. Diferentes peptídeos da gp43 foram adicionados a culturas de macrófagos derivados de medula óssea. Posteriormente, foram desafiados com zymosan e os índices fagocíticos foram determinados. A expressão dos peptídeos na superfície da molécula foi determinada por análises gráficas usando o módulo Protean; DNAstar Inc. Dois peptídeos que diminuíram índices fagocíticos e estavam expressos na superfície da gp43, P4 e P23, foram selecionados para ensaios posteriores. Mostramos que ambos, P4 e P23 inibiram a liberação de NO por macrófagos estimulados com zymosan enquanto aumentaram liberação de H2O2. A liberação de TNF-α no sobrenadante de cultura de testes fagocíticos in vitro também foi inibida na presença dos peptídeos. Ensaios in vivo com Mycobacterium bovis - bacillus Calmette-Guérin (BCG) demonstraram que estes peptídeos também apresentaram propriedades antiinflamatórias não-específicas a PCM.
- ItemSomente MetadadadosStructural and functional properties of the Trichosporon asahii glucuronoxylomannan(Elsevier B.V., 2009-06-01) Fonseca, Fernanda L.; Frases, Susana; Casadevall, Arturo; Fischman-Gompertz, Olga [UNIFESP]; Nimrichter, Leonardo; Rodrigues, Marcio L.; Universidade Federal do Rio de Janeiro (UFRJ); Albert Einstein Coll Med; Universidade Federal de São Paulo (UNIFESP)The virulence attributes of Trichosporon asahii are virtually unknown, despite its growing relevance as causative agent of superficial and invasive diseases in humans. Glucuronoxylomannan (GXM) is a well described virulence factor of pathogenic species in the Cryptococcus genus. GXM is also produced by species of the Trichosporon genus, and both polysaccharides share antigenic determinants, but unlike cryptococcal GXM, relatively little work has been done on trichosporal GXMs. in this study, we analyzed structural and functional aspects of GXM produced by T. asahii and compared them to the properties of the cryptococcal polysaccharide. Trichosporal and cryptococcal GXM shared antigenic reactivity, but the former polysaccharide had smaller effective diameter and negative charge. GXM anchoring to the cell wall was perturbed by dimethylsulfoxide and required interactions of chitin-derived oligomers with the polysaccharide. GXM from T asahii supernatants are incorporated by acapsular mutants of Cryptococcus neoformans, which renders these cells more resistant to phagocytosis by mouse macrophages. in summary, our results establish that despite similarities in cell wall anchoring, antigenic and antiphagocytic properties, trichosporal and cryptococcal GXMs manifest major structural differences that may directly affect polysaccharide assembly at the fungal surface. (C) 2009 Elsevier Inc. All rights reserved.