Navegando por Palavras-chave "Perfusion"
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- ItemSomente MetadadadosAvaliação, através da tonometria gástrica, do efeito da dobutamina na perfusão esplancnica no pôs operatório de cirurgia cardíaca em pediatria(Universidade Federal de São Paulo (UNIFESP), 1996) Souza, Renato Lopes de [UNIFESP]; Carvalho, Werther Brunow de [UNIFESP]
- ItemAcesso aberto (Open Access)Comparison of lactate values obtained from different sites and their clinical significance in patients with severe sepsis(Associacao Paulista Medicina, 2011-01-06) Nascente, Ana Paula Metran [UNIFESP]; Assunção, Murillo [UNIFESP]; Guedes, Carla Janaina [UNIFESP]; Freitas, Flavio Geraldo Rezende [UNIFESP]; Mazza, Bruno Franco [UNIFESP]; Jackiu, Miriam [UNIFESP]; Machado, Flavia Ribeiro; Universidade Federal de São Paulo (UNIFESP)CONTEXT AND OBJECTIVE: The ideal site for lactate collection has not been clearly established. This study aimed to evaluate associations between lactate levels in arterial blood (Lart), peripheral venous blood (Lper) and central venous blood (Lean) in patients with severe sepsis or septic shock.DESIGN AND SETTING: Cross-sectional analytical study in an tertiary university hospital.METHOD: Samples from patients with a central venous catheter and from healthy volunteers (control group) were collected. Blood was drawn simultaneously for measurements of Lart, Lper and Lcen, and the first sample was collected less than 24 hours after the onset of organ dysfunction. The results were analyzed using Pearson correlation, Bland-Altman and McNemar tests.RESULTS: A total of 238 samples were collected from 32 patients. The correlation results were r = 0.79 (P < 0.0001) for Lart/Lper and r = 0.84 (P < 0.0001) for Lart/Lcen. Bland-Altman showed large limits of agreement: -3.2 +/- 4.9 (-12.8 to 6.4) and -0.8 +/- 5.9 (-12.5 to 10.8), for Lper and Lcen respectively. lathe control group, there was greater correlation (r = 0.9009, P = 0.0004) and agreement: -0.7 +/- 1.2 (-3.1 to 1.7). Regarding clinical intervention, there was good agreement between Lart/Lcen (96.3%; three disagreements), with worst results for Lart/Lper (87.0%) with 10 cases of disagreement (P = 0.04). In eight patients (80.0%) Lper was higher than Lart.CONCLUSION: Lcen, and not Lper, can replace Lart with good correlation and clinical agreement. Lper tends to overestimate Lart, thus leading to unnecessary therapeutic interventions.
- ItemSomente MetadadadosEnzimas aminopeptidase- P e dipeptidilaminopeptidase IV na metabolização pulmonar de cininas em ratos normotensos e espontaneamente hipertensos(Universidade Federal de São Paulo (UNIFESP), 1992) Pesquero, João Bosco [UNIFESP]; Lindsey, Charles Julian [UNIFESP]
- ItemSomente MetadadadosInfluência da resposta imune do intestino no estado inflamatório sistêmico e na evolução clínica em ratos submetidos a um desafio microbiano agudo de origem intestinal e ou sanguíneo(Universidade Federal de São Paulo (UNIFESP), 2008) Díaz, José Luis Menchaca [UNIFESP]; Koh, Ivan Hong Jun [UNIFESP]
- ItemAcesso aberto (Open Access)Perfusão da artéria pulmonar não melhora os índices de fator natriurético cerebral (BNP) em estudo experimental em suínos(Sociedade Brasileira de Cirurgia Cardiovascular, 2010-12-01) Gabriel, Edmo Atique [UNIFESP]; Locali, Rafael Fagionato; Matsuoka, Priscila Katsumi; Almeida, Ludmila Santiago; Silva, Paulo Sérgio Venerando; Ishigai, Marcia Marcelino de Souza [UNIFESP]; Salerno, Tomas; Buffolo, Enio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de Miami Divisão de Cirurgia CardiotorácicaOBJECTIVE: Assess if the main pulmonary artery controlled perfusion over cardiopulmonary bypass (CPB) modifies BNP levels expressed by the ventricular myocardium. METHODS: Experimental research involving 32 pigs, divided into two groups according to CPB strategy - group I (cardioplegia) and group II (beating heart). Both groups were allocated into three subgroups according to lung perfusion strategy - subgroup A (control: no lung perfusion), subgroup B (lung perfusion with arterial blood) and subgroup C (lung perfusion with venous blood). In subgroups B and C, lung was perfused for 30 minutes, using preoperative mean pulmonary artery pressure (MPAP) as perfusion pressure, which was monitored through manometer. MPAP and pulmonary vascular resistance (PVR) were measured after coming off CPB using Swan-Ganz catheter. At preoperative time and 30 minutes after lung perfusion, specimens were taken from the right ventricular myocardium aiming to assess brain natriuretic peptide (BNP) and histologic pattern. Immunohistochemical and hematoxylin-eosin techniques were used to determine, respectively, BNP expression and inflammatory myocardial lesions. RESULTS: In animals submitted to controlled lung perfusion, there was a postoperative reduction of MPAP (P=0.03) and PVR (P=0.005).There was no differences among subgroups within the group, I (P=0.228) and subgroups within group II (P=0.325) as to postoperative BNP expression. There were no differences among subgroups with and without lung perfusion as to postoperative inflammatory lesions (P>0.05). CONCLUSION: Main pulmonary artery controlled perfusion for 30 minutes did not yield substantial modifications in BNP expression and histologic pattern of the right ventricular myocardium.
- ItemSomente MetadadadosPerfusão do fígado de rato com triton X-100: remoção e caracterização de uma aminopeptidase cinino-conversora de uma arilamidase(Universidade Federal de São Paulo (UNIFESP), 1980) Termignoni, Carlos [UNIFESP]; Guimarães, Jorge Almeida [UNIFESP]
- ItemAcesso aberto (Open Access)Perfusão por tomografia computadorizada do abdome: aplicações clínicas, princípios e técnica do exame(Colégio Brasileiro de Radiologia e Diagnóstico por Imagem, 2012-02-01) Sousa, João Paulo Lira Barros Almeida de [UNIFESP]; Bekhor, Daniel [UNIFESP]; Saito Filho, Celso Fernando [UNIFESP]; Bretas, Elisa Almeida Sathler [UNIFESP]; D'Ippolito, Giuseppe [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)New imaging techniques have been developed with the objective of obtaining not only a structural assessment, but also a metabolic and functional analysis of different organs and types of lesions. Among such tools perfusion computed tomography (PCT) has recently aroused the interest of many researchers in studying the applicability of such imaging modality in the evaluation of abdominal organs and diseases. Analysis of the biological behavior of healthy and diseased tissues, differentiation of inflammatory processes from tumors, and diagnosis of tumor recurrence after minimally invasive therapies can be mentioned as applications of such an imaging modality. The main features of PCT are related to its ability to characterize different perfusional behaviors reflecting biological changes of certain lesions and diseased tissues. Thus, the present study was aimed at carrying out a comprehensive literature review, describing the key techniques and protocols utilized in PCT imaging, main clinical applications, advantages and disadvantages of the method, besides proposing an imaging protocol that can be adopted by both private and public health services, with good reproducibility and easy implementation.
- ItemAcesso aberto (Open Access)Radioisotopic perfusional assessment of blood circulation changes in skin under progressive expansion: experimental model with rabbits(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2004-12-01) Horibe, Edith Kawano [UNIFESP]; Horibe, Kose; Universidade Federal de São Paulo (UNIFESP); of São Paulo University Faculty of MedicineThe purpose of this experimental model with rabbits is investigating the variation of blood flow in the expanded skin versus expansion time. New Zealand breed rabbits are used. Two groups are studied: F-1 receiving expanders on the right tight and F-2 receiving expanders bilateraly. Progressively, five expansions are performed. The first radioiosotopic perfusional evaluation is performed just after the surgery and the following evaluation are performed at the second, sixth, thirteenth, twentieth and twenty-seventh post-surgical days. As radiotracer, technetium 99m are used in the chemical form of sodium pertechnetate. Scintillographic images are obtained by CGR scintillation camera. The quantitative analysis is done by calculation of the reperfusion rate.
- ItemSomente MetadadadosTransporte transepitelial de glicose, sódio e água e RNA mensageiro dos transportadores de glicose no intestino delgado de ratos com anemia ferropriva(Universidade Federal de São Paulo (UNIFESP), 2001) Wayhs, Monica Lisboa Chang [UNIFESP]; Morais, Mauro Batista de [UNIFESP]Objetivos: Avaliar em ratos com anemia por defiCiência de ferro o transporte transepitelial de sodio, glicose, potassio e agua, e o conteudo de mRNA da proteina cotransportadora de sodio / glicose (SGLT1) e da proteina facilitadora do transporte de glicose (GLUT2) no intestino delgado. r Metodos: Foram utilizados, para cada experimento, ratos machos Wistar-EPM com 21I dias completos de vida, que receberam dieta pobre em ferro (<5 mg de ferro elementar/kg - grupo anemico) ou com conteudo normal de ferro (>35 mg de ferro elementar/kg - grupo controle) durante seis semanas. O transporte transepitelial de sodio, glicose, potassio e agua foi avaliado por meio do modelo experimental da perfusao intestinal In vivo em ratos com anemia ferropriva (n=9) e ratos sem anemia (n=10). A perfusao intestinal In vivo foi realizada em 20 cm de jejuno, utilizando-se a solucao de reidratacao da O.M.S. Em 12 ratos anemicos e 11 ratos sem anemia foram avaliados o conteudo relativo do mRNA do SGLT1 e do GLUT2 nas porcoes proximal, intermedia e distal do intestino delgado, por meio da tecnica de Northem Blotting. Os dois experimentos foram realizados em periodos e com ratos diferentes. Resultados: Apos seis semanas de tratamento dietetico a concentracao sanguinea da hemoglobina e o conteudo de ferro hepatico dos ratos anemicos submetidos a perfusao intestinal (Hb: 8,7 g/dl / DP=1,0 g/dl; ferro hepatico: 0,099 mg/g / DP=0,012 mg/g) ou utilizados para determinacao do mRNA (Hb: 3,9 g/dl / DP=0,7 g/dl; ferro hepatico: 0,078 mg/g / DP=0,015 mg/g) foram estatisticamente inferiores a dos ratos controles, tanto no primeiro experimento (Hb: 13,9 g/dl I DP=1,5 g/dl; ferro hepatico: 0,344 mg/g I DP=0,074 mg/g) quanto no segundo (Hb: 11,7 g/dl / DP=1,3 g/dl; ferro hepatico: 0,192 mg/g I DP=0,030 mg/g). O transporte transepitelial medio de sodio (-32,96 pEqlmin.cm / DP=370,81 pEq/min.cm), glicose (425,95 pM/min.cm / DP=535,84 gMlmin.cm) e agua (0,44 pl/min.cm / DP=4,18 pl/min.cm) no intestino delgado dos ratos anemicos foi significantemente menor do que o do grupo controle (sodio: 349,13 pEq/min.cm / DP=227,26 pEq/min.cm; glicose: 842,62 pM/min.cm / DP=297,64 gMlmin.cm; agua: 4,32 pl/min.cm / DP=2,79 pl/min.cm). Nao foi observada diferenca com relacao ao transporte transepiteliaL de potassio. O conteudo relativo de mRNA, expresso em unidades arbitrarias (UA), do SGLT1 dos ratos anemicos nos segmentos...(au)
- ItemAcesso aberto (Open Access)Utilização de cateter em veia femoral para avaliação de parâmetros de perfusão(Associação de Medicina Intensiva Brasileira - AMIB, 2013-06-01) Marti, Yara Nishiyama [UNIFESP]; Machado, Flávia Ribeiro [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The use of central venous oxygen saturation (SvcO2) and arterial lactate in the diagnosis of severe tissue hypoperfusion is well established, and the optimization of these parameters is currently under investigation, particularly in patients with severe sepsis/septic shock. However, the only place for deep venous puncture or the first choice for puncture is often the femoral vein. Although venous saturation obtained from blood sampling from this catheter, instead of SvcO2, has already been used in the diagnosis of severe tissue hypoperfusion, little is known about the accuracy of the results. The venous lactate in place of arterial puncture has also been used to guide therapeutic decisions. We conducted this literature review to seek evidence on the correlation and concordance of parameters obtained by collecting femoral venous blood gases in relation to SvcO2 and arterial lactate. Few studies in the literature have evaluated the use of femoral venous oxygen saturation (SvfO2) or venous lactate. The results obtained thus far demonstrate no adequate agreement between SvfO2 and SvcO2, which limits the clinical use of SvfO2. However, the apparent strong correlation between arterial and peripheral and central venous lactate values suggests that venous lactate obtained from the femoral vein could eventually be used instead of arterial lactate, although there is insufficient evidence on which to base this procedure at this time.
- ItemAcesso aberto (Open Access)Validação de um novo sistema de manometria esofágica de alta resolução por perfusão de água(Universidade Federal de São Paulo (UNIFESP), 2019-11-28) Silva, Rogerio Mariotto Bitetti Da [UNIFESP]; Fernandes, Fernando Augusto Mardiros Herbella [UNIFESP]; http://lattes.cnpq.br/4035568020554599; http://lattes.cnpq.br/4518044580886540; Universidade Federal de São Paulo (UNIFESP)Introduction: High resolution esophageal manometry (MEAR) has brought benefits over conventional manometry. This study aims to determine and validate normal values in a new water perfusion HRM system. Material and Method: Normal values were defined from the study of 32 healthy volunteers (group 1). The values were validated by a study of 225 individuals who underwent HRM for clinical complaints, subdivided into: gastroesophageal reflux disease (GERD) (group 2), achalasia (group 3), systemic diseases (group 4) and dysphagia (group 5). The system used was water perfusion with 24 sensors (Multiplex, Alacer Biomedica, São Paulo, Brazil). For the upper esophageal sphincter (UES), the parameters studied were basal and relaxation pressures. The parameters of the esophageal body were: distal contractility integral (DCI), distal latency (DL) and break. The parameters of the lower esophageal sphincter (LES) were basal and relaxation pressures and integrated relaxation pressure (IRP). Results: Normal values were: upper esophageal sphincter (UES) basal pressure 16.7-184.37 (mmHg), DL: 6.2-9.1 (s), DCI: 82.72-3836.61 (mmHg .s.cm), break: <7.19 (cm), LES basal pressure: 4.89-37.16 (mmHg), IRP: 0.55-15.45 (mmHg). In group 2, a hypotonic LES was found in 49% of individuals with confirmed GERD and 28% in pH-negative GERD. In group 3, aperistalse was found in all individuals. In the group 4 aperistalsis was observed in all cases. And in group 5 only 1 (14%) had normal HRM. Conclusions: We determine the normality values. Normal values are discriminating against changes in esophageal motility, therefore clinically validated.