Navegando por Palavras-chave "Orchiectomy"
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- ItemAcesso aberto (Open Access)Análise dos aspectos funcionais dos espermatozoides e dos níveis de peroxidação lipídica no plasma seminal de pacientes portadores de tumor de células germinativas de testículo pré e pós-orquiectomia(Universidade Federal de São Paulo (UNIFESP), 2016-03-29) Andrade, Maria Beatriz Ribeiro de [UNIFESP]; Spaine, Deborah Montagnini [UNIFESP]; http://lattes.cnpq.br/1321284998759806; http://lattes.cnpq.br/5373251016652631; Universidade Federal de São Paulo (UNIFESP)Objective: To verify if quality of spermatozoa from men with testicular germ cell tumors is better before or after orchiectomy. Methods: This prospective study was carried out including 24 patients with testicular germ cell tumors. The patients provided one semen sample before they were submitted to unilateral orchiectomy, and one other semen sample 30 days after the surgery. After collection by masturbation and liquefaction, an aliquot of the semen sample was used for semen analysis and another was used to evaluate sperm mitochondrial activity, DNA fragmentation and acrosomal integrity. The remaining volume was centrifuged and the supernatant seminal plasma was used to evaluate lipid peroxidation levels. Mitochondrial activity was determined by a colorimetric stain (3,3? ? diaminobenzidine ? DAB) and classified as DAB I (all mitochondrial active), DAB II (more than 50% mitochondrial active), DAB III (less than 50% mitochondrial active) and DAB IV (all mitochondrial inactive). Sperm DNA fragmentation was assessed by an Alkaline Comet assay, in which sperm were classified as high DNA integrity (class I and II) to high DNA fragmentation (class III and IV). Acrossome integrity was determined by a fluorescent probe (PNA ? FITC) and assessed by fluorescent microscopy. Lipid peroxidation levels were quantified by measuring thiobarbituric acid reactive substances (TBARS) levels, and were described in TBARS nmol/mL. Pre and Post-orchiectomy sample were compared using a paired Student?s T test (normal distribution) or a paired Wilcoxon test, when appropriate (p?0.05). Results: No significant difference was observed in semen analysis. A significant decrease in DNA fragmentation (p=0.019) and lipid peroxidation (p=0.014), and an increase in mitochondrial activity (p=0.048) was observed after orchiectomy. Conclusions: Based on our findings, the semen quality from men with testicular germ cell tumors is better quality after orchiectomy.
- ItemAcesso aberto (Open Access)Antioxidant enzyme profile and lipid peroxidation products in semen samples of testicular germ cell tumor patients submitted to orchiectomy(Brazilian Soc Urol, 2017) Sposito, Camila [UNIFESP]; Camargo, Mariana [UNIFESP]; Tibaldi, Danielle Spinola [UNIFESP]; Barradas, Valeria [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]; Nichi, Marcilio; Bertolla, Ricardo Pimenta [UNIFESP]; Spaine, Deborah Montagnini [UNIFESP]Purpose: To determine enzymatic antioxidant and lipid peroxidation levels in seminal plasma of patients orchiectomized for testicular tumors. Materials and Methods: The study included 52 patients: 26 control men and 26 orchiectomized patients for testicular tumor, of which 12 men had seminoma tumor and 14 men non-seminoma tumor. After semen analysis performed according to the WHO guidelines, an aliquot of semen was centrifuged and the seminal plasma was collected. Lipid peroxidation was performed by thiobarbituric acid reactive substances (TBARS) assay and antioxidant profile was assessed by analyzing catalase, glutathione peroxidase (GPx) and superoxide anion (SOD) activities using colorimetric assays with a standard spectrophotometer. Data were tested for normality and compared using one-way ANOVA (p<0.05). Results: Seminoma and non-seminoma groups presented lower sperm concentration and morphology when compared to control group (p=0.0001). Both study groups (seminoma and non-seminoma) presented higher TBARS levels when compared to control group (p=0.0000013). No differences were observed for SOD (p=0.646) andGPx (p=0.328). It was not possible to access the enzymatic activity of catalase in any group. Conclusion: Patients with testicular tumor present increased semen oxidative stress, but no differences were observed in antioxidant levels, even after orchiectomy. This indicates that most likely an increased generation of oxidative products takes place in these patients.
- ItemAcesso aberto (Open Access)Avaliação do efeito precoce da orquiectomia radical unilateral no perfil de proteínas do plasma seminal de homens portadores de tumor de células germinativas de testículo(Universidade Federal de São Paulo (UNIFESP), 2019-11-06) Andrade, Maria Beatriz Ribeiro De [UNIFESP]; Spaine, Deborah Montagnini [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To evaluate the effect of orchiectomy on the seminal plasma proteomic profile of men with testicular germ cell tumors. Methods: Seventeen men with Testicular Germ Cell Tumors provided one semen sample before (Pre-orchiectomy) and another 30 days after orchiectomy (Post-orchiectomy). Following liquefaction, an aliquot was used for semen analysis and other one was centrifuged for collection of seminal plasma. The remaining volume was criopreservated. The seminal plasma was used to proteomic analysis .For semen analysis a Student’s t-test for paired samples was used and to proteomic analysis a one sample Student’s t-test was performed. For both analysis was adopted p˂0,05. Effect size was assessed using Cohen’s d coefficient. Results: No significant difference was observed in semen analysis. Two hundred and seven proteins were identified and quantified with high fidelity, of which five were increase in the pre-orchiectomy period and eight proteins were increase in post-orchiectomy period. Conclusion: Removal of the affect testis alters the seminal plasma molecular environment.
- ItemSomente MetadadadosEfeitos da adrenalectomia bilateral com associacao de gonadotrofina corionica sobre os niveis plasmaticos da testosterona de ratos previamente castrados por duas tecnicas diferentes(Universidade Federal de São Paulo (UNIFESP), 2000) Simao, Omar Pacheco [UNIFESP]; Ortiz, Valdemar [UNIFESP]Esse experimento objetivou estudar os niveis de testosterona plasmatica em ratos submetidos bilateralmente a orquiepididimectomia ou orquiectomia subcapsular, seguido de adrenalectomia bilateral e administracao de gonadotrofina corionica (HCG). Quarenta e oito ratos Wistar adultos foram distribuidos em 3 grupos. No grupo I (l6 animais) realizou-se a orquiepididimectomia, no, grupo II (l6 animais) realizou-se a orquiectomia subcapsular e no grupo III (l6 animais) realizou-se uma operacao simulada. Apos 3 meses, realizou-se adrenalectomia bilateral e administrava-se 100 UI de HCG. Coletou-se 1 mL de sangue e as amostras foram agrupadas de duas em duas, para dosagem de testosterona plasmatica antes da operacao, apos 3 meses da orquiectomia bilateral (Pos 1) e uma semana apos a adrenalectomia bilateral e HCG (Pos 2). Obteve-se respectivamente como media da testosterona plasmatica pre-operatoria, pos-orquiectomia (Pos 1) e pos adrenalectomia e HCG (Pos 2) os seguintes valores: Grupo I: 235,87 ng/dL, 158ng/dL (queda de 27,32 por cento) e 132,5 ng/dL (queda de 40,52 por cento). No Grupo II: 254,62 ng/dL, 158,0 ng/dL (queda de 45,81 por cento) e 132,5 ng/dL (queda de 38,43 por cento). No Grupo III: 265,88 ng/dL, 455,88 ng/dL (aumento de 59,89 por cento) e 471,13 ng/dL (aumento de 74,55 por cento). Conclui-se que a orquiepididimectomia ou a orquiectomia subcapsular reduzem igualmente os niveis plasmaticos de testosterona no rato, apos 3 meses de observacao e que a adrenalectomia bilateral seguido de estimulo com HCG tambem reduz igualmente os niveis de testosterona dos animais submetidos as duas tecnicas de orquiectomias
- ItemSomente MetadadadosEfeitos da orquiepididimectomia total e da orquiectomia subcapsular sobre os níveis plasmáticas de testosterona em ratos(Universidade Federal de São Paulo (UNIFESP), 1998) Simao, Omar Pacheco [UNIFESP]; Ortiz, Valdemar [UNIFESP]Esse experimento objetivou os niveis de testosterona plasmaticca em ratos submetidos a orquiepididimectomia total e orquiectomia subcapsular. Trinta e quatro ratos Wistar adultos foram distribuidos em 3 grupos, sendo que no grupo I (12 animais) realizou-se a orquiepididimectomia total, no grupo II (12 animais) realizou-se a orquiectomia subcapsular e no grupo III (10 animais) realizou-se uma operacao simulada. Todos os animais foram anestesiados com eter etilico por via inalatoria, submetidos a coleta de sangue para dosagem de testosterona plsmatica antes da operacao e apos 6 meses de pos-operatorio. Obteve-se no grupo I como media da testosterona plasmatica pre operatoria o valor de 190,7 ng(por cento) e no pos-operatorio 92,6 nh(por cento), o qual correspondeu a uma variacao porcentual media de -48,5(por cento). No grupo II obteve-se no pre-operatorio valor medio de 187,5 ng(por cento) e no pos-operatorio 82,2 ng(por cento), o qual correspondeu a uma variacao porcentual de -52,1(por cento). No grupo III obteve-se media de 183,0 ng(por cento) no pre-operatorio e no pos-operatorio de 168,6 ng(por cento), o qual correspondeu a uma variacao porcentual media de -15,4(por cento). Concluiu-se que a orquiepididimectomia total e a orquiectomia subcapsular reduziram igualmente os niveis plasmaticos de testosterona no rato, apos 6 meses de observacao