Navegando por Palavras-chave "Nontuberculous mycobacteria"
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- ItemSomente MetadadadosDiversidade de micobactérias de ambientes aquáticos e seu potencial biotecnológico(Universidade Federal de São Paulo, 2017-07-05) Romagnoli, Camila Lopes [UNIFESP]; Niero, Cristina Viana [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Non-tuberculous mycobacteria (NTM) are found in many natural and human-influenced environments. Most species are saprophytic, but some are considered opportunistic pathogens because they can cause infections in humans and animals. Some years ago, NTMs were noticeable in studies in the biotechnology area due to the description of isolates that metabolize toxic pollutants. Currently, the genus has 185 species, of which 94 (56.2%) have been described since 2000. Isolation and identification works report difficulties in the identification of environmental mycobacterial isolates, demonstrating the diversity of the genus and suggesting the existence of species not described yet. Within this context, this project aimed to identify 382 mycobacterial isolates from aquatic environments located at the Zoological Park Foundation of São Paulo. All isolates had a 401bp fragment of the hsp65 gene sequenced for identification and / or initial grouping. Isolates not identified by this target had the V region of the rpoB gene and the complete 16S rRNA gene sequenced. As a result, 177 (46%) isolates were identified and distributed in 18 species, being M. insubricum, M. gordonae and M. chelonae were the most prevalent species. Among the 205 unidentified isolates, 114 (30%) were grouped into complexes, being 73 belonging to the M. terrae complex, 37 to the M. fortuitum complex, two to the M. avium complex and two to the M. simiae complex. Still, 91 isolates (24%) were not identified. These results confirm the diversity of MNT isolates from environmental samples and reinforce the suggestion about the existence of new species belonging to the genus Mycobacterium. Among the isolates, we identified groups with biotechnological potential for degradation of pectin and starch, in addition to species not yet reported in the aquatic environment. The analysis of the collection sites revealed the tendency to find greater abundance and diversity of species in eutrophic environments, in comparison to the non-eutrophic ones. Thus our results contribute to the knowledge about the abundance and biodiversity of mycobacteria in the aquatic environments analyzed and point to the existence of isolates with biotechnological potential, areas of research where there is still little knowledge regarding the performance of environmental mycobacteria
- ItemSomente MetadadadosEmergence of nosocomial Mycobacterium massiliense infection in Goias, Brazil(Elsevier B.V., 2008-11-01) Cardoso, Alessandra Marques; Sousa, Eduardo Martins de; Viana-Niero, Cristina [UNIFESP]; Bortoli, Fernando Bontim de; Pereira das Neves, Zilah Candida; Leao, Sylvia Cardoso [UNIFESP]; Junqueira-Kipnis, Ana Paula; Kipnis, Andre; Universidade Federal de Goiás (UFG); Universidade Federal de São Paulo (UNIFESP); Secretaria Municipal Saude GoianiaA cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiania, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiania apart from this period. the 18 isolates were identified as Mycobacterium massiliense by PCR-restriction digestion of the hsp65 gene, pulsed-field gel electrophoresis (PFGE) comparisons, and rpoB partial gene sequencing. All isolates were typed as a single clone, indicating that they have the same origin, which suggests a common source of infection for all patients. (C) 2008 Elsevier Masson SAS. All rights reserved.
- ItemSomente MetadadadosFollow-up on an outbreak in Venezuela of soft-tissue infection due to Mycobacterium abscessus associated with Mesotherapy(Ediciones Doyma S A, 2010-11-01) Da Mata Jardin, Omaira; Hernandez-Perez, Rolando; Corrales, Haidee; Cardoso-Leao, Sylvia [UNIFESP]; Waard, Jacobus H. de; Inst Biomed; Hosp Gen Dr Luis Razetti; Universidade Federal de São Paulo (UNIFESP)Introduction: Skin and soft tissue infections caused by nontuberculous mycobacteria (NMT) are reported to be associated with injections, liposuction, plastic surgery, and acupuncture. Herein, we describe an outbreak of soft tissue infection due to NMT following mesotherapy, a cosmetic procedure involving injection of poorly defined mixtures alleged to reduce local adiposity.Methods: Patients with skin lesions and a history of mesotherapy treatment, who visited the dermatology department of the public hospital in Barinas, Venezuela, from November 2004 to February 2005 were interviewed. Clinical and environmental samples were taken for mycobacteria isolation.Results: the interviews revealed that 68 patients who had been treated for cosmetic purposes at the same clinic by the same therapist had received injections with the same product and were infected with NMT. Clinical specimens from 5 patients grew Mycobacterium abscessus. No mesotherapy solution was available for analysis but M. abscessus was isolated from an environmental sample in the clinic. PCR-based strain typing techniques (ERIC-PCR, BOXA1R and RAPD) showed that the patient's isolates were undistinguishable from each other but different from the environmental isolate.Conclusions: This outbreak was likely caused by a contaminated injectable mesotherapy product and not by mycobacteria from the clinic environment. We emphasize the importance of better microbiological control of these products. To our knowledge, this outbreak, which affected at least 68 patients, appears to be the largest ever associated with mesotherapy and described in the literature. (C) 2009 Elsevier Espana, S.L. All rights reserved.