Repositório Institucional - Unifesp :: Navegando por Palavras-chave "Neuropeptides"

Navegando por Palavras-chave "Neuropeptides"

Agora exibindo 1 - 20 de 22

Somente Metadadados
Aerobic training (AT) is more effective than aerobic plus resistance training (AT plus RT) to improve anorexigenic/orexigenic factors in obese adolescents
(Elsevier B.V., 2013-10-01) Carnier, June [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Ackel-D'Elia, Carolina [UNIFESP]; Corgosinho, Flavia Campos [UNIFESP]; Campos, Raquel Munhoz da Silveira [UNIFESP]; Sanches, Priscila de Lima [UNIFESP]; Masquio, Deborah Cristina Landi [UNIFESP]; Bueno Junior, Carlos Roberto [UNIFESP]; Piano, Aline de [UNIFESP]; Martins, Aniela C. [UNIFESP]; Caranti, Danielle Arisa [UNIFESP]; Tock, Lian [UNIFESP]; Clemente, Ana Paula Grotti [UNIFESP]; Tufik, Sergio [UNIFESP]; Dâmaso, Ana Raimunda [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Background: the regulation of energy balance is influenced by physical exercise. Although some studies show a stimulation of hormones related to food intake, others show that exercise provides satiety. Aim: the aim of this study was to compare the effects of aerobic training (AT) and aerobic plus resistance training (AT + RT) on anorexigenic and orexigenic factors in obese adolescents undergoing interdisciplinary weight loss therapy. Methods: A total of 26 obese adolescents, aged 15-19 years with BMI >= P95 were submitted to 12 months of interdisciplinary intervention (clinical support, nutrition, psychology and physical exercise) and divided into two groups, aerobic training (AT) (n = 13) or aerobic plus resistance training (AT + RT) (n = 13), which were matched according to gender and body mass. Blood samples were collected to analyze orexigenic factors (AgRP, NPY, MCH) and the anorexigenic factor alpha-MSH. Results: the AT and AT + RT groups significantly reduced body mass, body mass index and body fat mass (kg) during the therapy. the AT group showed no significant changes in body lean mass (kg), whereas the AT + RT group showed an increase in body lean mass (kg) during the interdisciplinary intervention. There was an increase in AgRP levels (ng/ml) only in the AT + RT group after 6 months of interdisciplinary intervention compared with baseline condition. Conversely, alpha-MSH levels (ng/ml) increased only in the AT group after 12 months of interdisciplinary intervention compared with baseline condition. Conclusion: Aerobic training (AT) as part of an interdisciplinary therapy is more effective than aerobic plus resistance training (AT + RT) to improve secretion of anorexigenic/orexigenic factors in obese adolescents. (C) 2013 Elsevier B.V. All rights reserved.
Somente Metadadados
Alterations in Downstream Mediators Involved in Central Control of Eating Behavior in Obese Adolescents Submitted to a Multidisciplinary Therapy
(Elsevier B.V., 2011-09-01) Prado, Wagner Luiz do [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Lofrano-Prado, Mara Cristina [UNIFESP]; Piano, Aline de [UNIFESP]; Stella, Sérgio Garcia [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Carnier, June [UNIFESP]; Caranti, Danielle Arisa [UNIFESP]; Tock, Lian [UNIFESP]; Tufik, Sergio [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Dâmaso, Ana Raimunda [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Objective: the aim of this study was to verify the effects of a multidisciplinary therapy (24 weeks) on neurohormonal control of food intake, specifically in orexigenic (total ghrelin, agouti-related protein [AgRP], neuropeptide Y [NPY], and melanin-concentrating hormone) and anorexigenic factors (leptin, insulin, and alpha-melanocyte stimulating hormone [alpha-MSH]), in obese adolescents.Methods: A total of 88 adolescents (38 boys and 50 girls), including 62 obese and 26 normal-weight, aged 15-19 years were recruited. Obese adolescents were submitted to a 24-week multidisciplinary therapy. AgRP, NPY, melanin-concentrating hormone, leptin, insulin, glucose, alpha-MSH, total ghrelin, and food intake were measured at three stages (at baseline, after 12 weeks, and after 24 weeks).Results: At baseline, obese adolescents showed hyperleptinemia (circulating leptin levels, which were, in boys and girls, 40 and 35 times higher than in normal-weight subjects, respectively). After 24 weeks, these values decreased in all obese patients. Our results showed no differences in ghrelin levels between obese and normal-weight adolescents, in both genders. However, obese boys reduced their plasma ghrelin concentration after 24 weeks of therapy (p<.05). the multidisciplinary therapy decreased NPY and AgRP values and increased alpha-MSH; simultaneously with these changes there was a decrease in total food intake after 24 weeks of therapy.Conclusions: We can conclude that the multidisciplinary therapy was efficient to modulate neurohormonal control of food intake in obese adolescents. (C) 2011 Society for Adolescent Health and Medicine. All rights reserved.
Acesso aberto (Open Access)
Capsaicin on the viability of random-pattern skin flaps in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2010-10-01) Godoy, Gustavo Roberto de [UNIFESP]; Liebano, Richard Eloin [UNIFESP]; Corrêa, Juliana Barbosa [UNIFESP]; Hochman, Bernardo [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
PURPOSE: To evaluate the effects of capsaicin on the viability of ischemic random-pattern skin flaps in rats. METHODS:Forty EPM1-Wistar rats were randomized into two groups of 20 animals each, the capsaicin group and the control group. A random-pattern skin flap measuring 10 x 4cm was raised and a plastic barrier was placed between the flap and the donor site. After the surgical procedure, the control group was treated with an inert vehicle in the form of a cream applied uniformly to a rayon bandage which, in turn, was applied to the surface of the skin flap. The capsaicin group was treated in the same way, but in this case capsaicin was added to the cream. This procedure was repeated for two consecutive days. RESULTS: There was a significantly smaller amount of flap necrosis in the capsaicin group (35.07%) than in the control group (44.75%) (p=0.035). CONCLUSION:Topical administration of capsaicin improved the viability of ischemic random-pattern skin flaps in rats.
Somente Metadadados
Cardiovascular effects of a specific nonpeptide antagonist of substance-p (nk-1) receptor in doca-salt hypertension
(Amer Heart Assoc, 1995-12-01) Kohlmann Junior, Osvaldo [UNIFESP]; Ginoza, Milton [UNIFESP]; Cesaretti, Mario Luis Ribeiro [UNIFESP]; Zanella, Maria Teresa [UNIFESP]; Ribeiro, Artur Beltrame [UNIFESP]; Ramos, Oswaldo Luiz [UNIFESP]; Leeman, Susan E.; Gavras, Irene; Gavras, Haralambos [UNIFESP]; Tavares, Agostinho [UNIFESP]; BOSTON UNIV; Universidade Federal de São Paulo (UNIFESP)
The neurotransmitter substance P acts also as a potent vasodilator. Its participation in the pathogenesis of deoxycorticosterone acetate (DOCA)-salt hypertension was evaluated by an acute infusion of a newly synthesized, potent, specific nonpeptide antagonist of substance P at the NK-1 receptor, the agent CP 96 345. In conscious unrestrained rats, CP 96 345 induced significant and sustained increases in mean arterial pressure of DOCA-salt rats but only small, transient, and nonsignificant rises in blood pressure of sham-treated control rats. The rise in blood pressure was not accompanied by changes in heart rate. Maximal blood pressure increase in DOCA-salt rats was 31.7+/-14.8 mm Hg. In a second series of experiments, the hemodynamic effects of this antagonist were evaluated under anesthesia in both DOCA-salt and sham-treated control rats by the thermodilution method. During CP 96 345 infusion, sustained increases in cardiac index and stroke volume and decreases in total peripheral resistance were observed in both DOCA-salt and control rats. In DOCA-salt rats, cardiac index rose by 79.4%, while total peripheral resistance fell by 27.9% of the baseline values. In control rats, the changes were smaller (+27.2% and -22.5%, respectively). Stroke volume changed in parallel to cardiac output in both groups. The data suggest that acute blockade of NK-1 receptors increases blood pressure in DOCA-salt rats mainly by an increase in cardiac output. We conclude that endogenous substance P tends to counteract the DOCA-salt-induced elevation of blood pressure by modulating both cardiac output and peripheral resistance.
Somente Metadadados
Chronic Exercise Promotes Alterations in the Neuroendocrine Profile of Elderly People
(Georg Thieme Verlag Kg, 2012-12-01) Alves, Eduardo da Silva [UNIFESP]; Souza, Helton de Sá [UNIFESP]; Rosa, João Paulo Pereira [UNIFESP]; Lira, Fabio Santos de [UNIFESP]; Pimentel, Gustavo Duarte; Santos, Ronaldo Vagner Thomatieli dos [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Dâmaso, Ana Raimunda [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Viana, Valter Antonio Rocha [UNIFESP]; Boscolo, Rita Aurélia [UNIFESP]; Grassmann, Viviane [UNIFESP]; Santana, Marcos Gonçalves de [UNIFESP]; Tufik, Sergio [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Ctr Estudo Psicobiol Exercicio; Universidade Federal de São Paulo (UNIFESP); Univ Extremo Sul Catarinense; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de Goiás (UFG)
Aging and physical inactivity are 2 factors that favour the development of cardiovascular disease, metabolic syndrome, obesity, and diabetes. in contrast, adopting a habitual moderate exercise routine may be a nonpharmacological treatment alternative for neuroendocrine aging disorders. We aimed to assess the effects of moderate exercise training on the metabolic profiles of elderly people with sedentary lifestyles. Fourteen sedentary, healthy, elderly male volunteers participated in a moderate training regimen for 60 min/day, 3 days/week for 24 weeks at a work rate equivalent to their ventilatory aerobic threshold. the environment was maintained at a temperature of 23 +/- 2 degrees C, with a humidity of 60 +/- 5 %. Blood samples for analysis were collected at 3 intervals: at baseline (1 week before training began), and 3 and 6 months after training. the training promoted increased aerobic capacity (relative VO2, and time and velocity to VO(2)max; (p < 0.05)) and reduced serum alpha-MSH (p < 0.05) after 3 months of training when compared with the baseline data. in addition, serum thyroid hormone (T3 and T4) was reduced after 6 months of training compared with baseline levels. Our results demonstrate that a moderate exercise training protocol improves the metabolic profile of older people, and metabolic adaptation is dependent on time.
Acesso aberto (Open Access)
Controle neuroendócrino da massa óssea: mito ou verdade?
(Sociedade Brasileira de Endocrinologia e Metabologia, 2003-08-01) Borba, Victoria Zeghbi Cochenski [UNIFESP]; Kulak, Carolina Aguiar Moreira; Lazaretti-Castro, Marise [UNIFESP]; Universidade Federal do Paraná Hospital de Clínicas Serviço de Endocrinologia e Metabologia; Universidade Federal de São Paulo (UNIFESP)
Bone remodeling is a physiologic process regulated by the interaction between the bone cells and a variety of hormones, cytokines, growth factors and inflammatory mediators. The central nervous system (CNS) has been proposed as a new regulatory element, acting through the release of neuronal messengers, which create a link between CNS and skeleton. There have been experimental and clinical evidence of neuroendocrine control of bone mass, with several factors implicated in this mechanism, including neuropeptides, neurotransmitters, leptin and others. Clinically, participation of neuroendocrine mechanisms comes from observation of bone loss in hypothalamic-pituitary disorders. More recently, leptin deficiency and leptin resistance have also been involved with bone metabolism. New studies are necessary to improve our knowledge on the relationships between these two important systems, and to establish if there is a local or systemic participation of the neuroendocrine system in bone metabolism.
Somente Metadadados
Early effects of Roux-en-Y gastric bypass on peptides and hormones involved in the control of energy balance
(Lippincott Williams & Wilkins, 2016) Dal Molin Netto, Bárbara [UNIFESP]; Earthman, Carrie P.; Bettini, Solange Cravo; Clemente, Ana Paula Grotti [UNIFESP]; Masquio, Deborah Cristina Landi [UNIFESP]; Farias, Gisele; Boritza, Katia; Silva, Larissa Gabrielle da; von der Heyde, Maria Emilia; Dâmaso, Ana Raimunda [UNIFESP]
Introduction Body weight varies depending on the prevailing direction of environmental pressures
Somente Metadadados
Effects of Lidocaine, Bupivacaine, and Ropivacaine on Calcitonin Gene-Related Peptide and Substance P Levels in the Incised Rat Skin
(Lippincott Williams & Wilkins, 2016) Lapin, Guilherme Abbud Franco [UNIFESP]; Hochman, Bernardo [UNIFESP]; Maximino, Jessica Ruivo; Chadi, Gerson; Ferreira, Lydia Masako [UNIFESP]
OBJECTIVE: To evaluate the effect of 2% lidocaine, 0.5% bupivacaine, and 0.75% ropivacaine on the release of substance P (SP) and calcitonin gene-related peptide (CGRP) in skin wounds. DESIGN: A primary, experimental, analytical, prospective, self-controlled, blinded study. SETTING: The study is set in a university research center. INTERVENTIONS: Twenty-eight Wistar rats were randomly divided into 4 groups: lidocaine, bupivacaine, ropivacaine, and the control. After general anesthesia, a local anesthetic or 0.9% saline (control) was injected subdermally along a 2-cm line on the dorsal midline of each rat; 30 minutes later, an incision (nociceptive stimulus) was made along this line. The animals were euthanized, and skin samples were collected from the center of the incision line and sent for CGRP and SP quantification. MAIN OUTCOME MEASURES: Quantification of CGRP and SP by Western blotting. RESULTS: Substance P levels were similar in the lidocaine and ropivacaine groups but were significantly lower than those of the control group (P = .002); no significant difference in SP levels was found between the bupivacaine and control groups. Procalcitonin gene-related peptide levels were significantly lower in the experimental groups than those in control subjects (P = .009), with no significant differences among the experimental groups. No significant differences in CGRP levels were found among all groups. Lidocaine and ropivacaine inhibited SP release. All 3 local anesthetics inhibited the release of procalcitonin gene-related peptide, but not the release of CGRP in rat skin. CONCLUSIONS: Lidocaine and ropivacaine may inhibit neurogenic inflammation by biochemical pathways activated by SP, whereas bupivacaine seems to have no influence on this process.
Acesso aberto (Open Access)
Effects of mindfulness-based interventions on biomarkers in healthy and cancer populations: a systematic review
(Biomed Central Ltd, 2017) Sanada, Kenji; Alda Diez, Marta; Salas Valero, Montserrat; Perez-Yus, Maria C.; Demarzo, Marcelo M. P. [UNIFESP]; Montero-Marin, Jesus; Garcia-Toro, Mauro; Garcia-Campayo, Javier
Background: Only a small number of articles have investigated the relationship between mindfulness-based interventions (MBIs) and biomarkers. The aim of this systematic review was to study the effect of MBIs on specific biomarkers (cytokines, neuropeptides and C-reactive protein (CRP)) in both healthy subjects and cancer patients. Methods: A search was conducted using PubMed, EMBASE, PsycINFO and the Cochrane library between 1980 and September 2016. Results: A total of 13 studies with 1110 participants were included. In the healthy population, MBIs had no effect on cytokines, but were found to increase the levels of the neuropeptide insulin-like growth factor 1 (IGF-1). With respect to neuropeptide Y, despite the absence of post-intervention differences, MBIs may enhance recovery from stress. With regard to CRP, MBIs could be effective in lower Body Mass Index (BMI) individuals. In cancer patients, MBIs seem to have some effect on cytokine levels, although it was not possible to determine which specific cytokines were affected. One possibility is that MBIs might aid recovery of the immune system, increasing the production of interleukin (IL)-4 and decreasing interferon gamma (IFN-gamma). Conclusions: MBIs may be involved in changes from a depressive/carcinogenic profile to a more normalized one. However, given the complexity and different contexts of the immune system, and the fact that this investigation is still in its preliminary stage, additional randomized controlled trials are needed to further establish the impact of MBI programmes on biomarkers in both clinical and non-clinical populations.
Acesso aberto (Open Access)
Estimulação elétrica na liberação do Peptídeo Relacionado com Gene da Calcitonina (CGRP) e Substância P (SP) em pele de ratos.
(Universidade Federal de São Paulo (UNIFESP), 2014) Antunes, Arainy Suély [UNIFESP]; Duailibi, Silvio Eduardo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Introdução: A disfunção na liberação de neuropeptídeos acarreta alterações na pele, podendo gerar distúrbios no processo cicatricial e/ou afecções cutâneas. Sendo assim, a liberação de neuropeptídeos é estudada na literatura, relacionando a utilização de agentes eletrofísicos. Objetivo: Investigar o efeito da estimulação elétrica na liberação de neuropeptídeos SP e CGRP em pele de ratos. Métodos: Foram utilizados 28 animais distribuídos em 4 grupos, Grupo Controle (GC): as amostras foram coletadas após 60 minutos da tricotomia sem estímulo elétrico; Grupo Sham (GS): após 60min da tricotomia foram colocadas as placas de eletrodos com esponja umedecida com cloreto de sódio 0,9% e sobreposto na linha mediana dorsal e dispersivo na região ventral, com o aparelho desligado por 30minutos; Grupo Estimulação Elétrica com Polo Positivo (GPP): após 60 minutos da tricotomia, foi realizada a estimulação elétrica sobre a linha mediana dorsal com polaridade Positiva e o no Grupo Estimulação Elétrica com polaridade Negativa (GPN): os mesmos parâmetros utilizados acima, com a mudança, onde o polo negativo passou para linha mediana dorsal. Ao término da estimulação elétrica, foram coletadas amostras de pele, submetidas ao Western blotting para análise dos neuropeptídeos CGRP e SP. Para a análise estatística foi utilizado o teste de Análise Variância (ANOVA) para identificar a diferença entre os grupos. Resultado: Não causou diferença significante na liberação de CGRP e SP na pele de rato. Conclusão: A estimulação elétrica ultraexcitante não influenciou na liberação de neuropeptídeos, CGRP e SP, em pele de ratos.
Acesso aberto (Open Access)
The high glycemic index diet was an independent predictor to explain changes in agouti-related protein in obese adolescents
(Aula Medica Ediciones, 2014-02-01) Dal Molin Netto, Bárbara [UNIFESP]; Masquio, Deborah Cristina Landi [UNIFESP]; Campos, Raquel Munhoz da Silveira [UNIFESP]; Sanches, Priscila de Lima [UNIFESP]; Corgosinho, Flavia Campos [UNIFESP]; Tock, Lian; Oyama, Lila Missae [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Tufik, Sergio [UNIFESP]; Dâmaso, Ana Raimunda [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
The high glycemic index diet was an independent predictor to explain changes in agouti-related protein in obese adolescents.Background & Aims: the role of diet glycemic index (GI) in the control of orexigenic and anorexigenic factors of the energy balance is still not clear. the present study aimed to assess whether the habitual diet, according to different GI foods, exerts influence on regulation of energy balance markers and the effects of interdisciplinary intervention in obese adolescents.Methods: A total of 55 obese adolescents, aged from 14 to 19 years, were submited to one year of interdisciplinary therapy and were divided in two groups, according to the predominant dietary pattern of food intake: high-GI group (H-GI; n = 29) and moderate/low-GI group (M/L-GI; n = 26).Results: the concentration of orexigenic factor AgRP (p < 0.01), visceral fat (p = 0.04) and visceral/subcutaneous ratio (p = 0.03) were higher in the group of H-GI when compared with M/L-GI group. Moreover, the habitual consumption of H-GI foods was an independent predictor to explain changes in AgRP concentrations. After one year of interdisciplinary therapy, the adolescents presented significant reductions in body weight, total body fat (%), visceral and subcutaneous fat and HOMA-IR, as well as a significant increase of fat free mass (%).Conclusions: Our results may suggest that habitual H-GI diet could upregulate orexigenic pathways, contributing to vicious cycle between undesirable diets, deregulates energy balance and predispose to obesity. One the other hand, one year of interdisciplinary therapy can significant improves metabolic profile and central obesity in adolescents.
Acesso aberto (Open Access)
Hyperleptinemia in obese adolescents deregulates neuropeptides during weight loss
(Elsevier B.V., 2011-07-01) Dâmaso, Ana Raimunda [UNIFESP]; Piano, Aline de [UNIFESP]; Sanches, Priscila de Lima [UNIFESP]; Corgosinho, Flávia Campos [UNIFESP]; Tock, Lian [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Tock, Luciana [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Tufik, Sergio [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Leptin has emerged over the past decade as a key hormone not only in energy balance regulation but also in neuroendocrine and inflammatory processes. the aim of the present study was to evaluate whether hyperleptinemia deregulates neuropeptides during weight loss. A total of 86 post-pubertal obese adolescents (with or without hyperleptinemia) participated in one year of interdisciplinary weight loss therapy (clinical, nutritional, psychological and exercise-related). Adipokine and neuropeptide concentrations were measured by ELISA, visceral fat was measured by ultrasound and body composition was measured by pletismography. the hyperleptinemic patients presented a lower alpha-MSH concentration and higher NPY/AgRP ratio while the adiponectin/leptin (A/L) ratio was lower compared with the non-hyperleptinemic group. After therapy, significant improvements in BM, BMI, body fat mass, visceral and subcutaneous fat, HOMA-IR, QUICKI, total cholesterol and triglycerides were observed in both groups. Indeed, we observed significant increases in adiponectin and A/L as well as reductions in leptin and NPY/AgRP ratio in the hyperleptinemic group. in the stepwise multiple linear regression analysis with leptin concentration as the dependent variable, alpha-MSH and body fat mass (%) were the independent predictors to explain leptin concentration. for the entire group, we found positive correlations between leptinemia and BMI and body fat mass (%) as well as a negative correlation with free fat mass (%) and alpha-MSH. Finally, we verified negative correlations between adiponectin/leptin ratio with total cholesterol and LDL-c, only in hyperleptinemic patients. in conclusion, the hyperleptinemia in obese adolescents deregulates neuropeptides during weight loss. (C) 2011 Elsevier Inc. All rights reserved.
Acesso aberto (Open Access)
Identificação de peptídeos intracelulares em cérebro de camundongos submetidos à privação e restrição de sono
(Universidade Federal de São Paulo (UNIFESP), 2016-04-30) Polster, Gabriela [UNIFESP]; Chagas, Jair Ribeiro [UNIFESP]; http://lattes.cnpq.br/2482076525999383; http://lattes.cnpq.br/6645137783851228; Universidade Federal de São Paulo (UNIFESP)
Neuropeptides have a fundamental role in the functioning of the whole organism and in particular in the central nervous system. The activity of a number of neuropeptides, especially interaction with their receptors has been widely studied. However, recently, intracellular peptides with biological activity have been found in several models. The identification and generation mechanisms of these assets intracellular peptides from inactive or active precursors and the mechanisms of action of these compounds has been less detailed in the literature. Our interest then turned to the identification and quantification of intracellular peptides in the brains of mice C57 / Bl6 controls or subjected to different sleep deprivation protocols. In our study we identified intracellular peptides and neuropeptides with special attention to the MAKVALIVILLFVL peptide, Melanopsin receptor fragment, which had not been identified in previous peptidomics studies
Somente Metadadados
LEPR polymorphism may affect energy balance during weight loss among Brazilians obese adolescents
(Churchill Livingstone, 2017) Corgosinho, Flavia Campos [UNIFESP]; Almeida, Sandro Soares [UNIFESP]; Tock, Lian; Pesquero, João Bosco [UNIFESP]; Araujo, Ronaldo Carvalho [UNIFESP]; Clemente, Ana Paula Grotti; Dal Molin Netto, Bárbara [UNIFESP]; Campos, Raquel Munhoz da Silveira [UNIFESP]; Masquio, Deborah Cristina Landi; Ferreira, Joana Pereira de Carvalho [UNIFESP]; Sanches, Priscila de Lima [UNIFESP]; Piano, Aline de; Rogero, Marcelo Macedo; Oyama, Lila Missae [UNIFESP]; Tufik, Sergio [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Dâmaso, Ana Raimunda [UNIFESP]
Objective: Leptin is an adipokine released mainly by adipose tissue, with many functions including regulation of energy balance. However, little is known about the effect of LEPR polymorphism on orexigenic and anorexigenic neuropeptides. Thus, the aim of the present study is to verify the influence of LEPR polymorphism (rs2767485) on serum orexigenic (NPY, MCH and AgRP) and anorexigenic (Leptin and alpha-MSH) neuropeptides levels among obese adolescents submitted to 1 year of multicomponent weight loss therapy. Methods: Seventy-six adolescents with obesity were enrolled in 1 year of weight loss therapy including clinical, nutritional, psychological and exercise-related. Blood samples were collected to analyze neuropeptides (NPY, MCH, AgRP and leptin) and LEPR genotyping. Visceral fat was measured by ultrasound and body composition was measured by plethysmography. The parameters were measured at baseline and after one year. Adolescents were grouped according to genotype (TT or CT + CC group). Effect of the weight loss therapy was analyzed through ANOVA and Wilcox, according to normality. Statistic value was set at < 0.05. Results: C-allele carriers have the orexigenic neuropeptides (NPY, AgRP and MCH) levels statistically higher when compared with TT group, at baseline. Furthermore, TT group seems to respond better to the therapy by a greater delta on BMI. Indeed, the data suggest a concomitant increased of AgRP levels in CT + CC genotypes, after weight loss therapy. Conclusion: Both groups responded to the weight loss intervention, however wildtypes (TT) appear to respond to the intervention most optimally with C carries, where post intervention reduction in BMI was significantly greater in wildtypes. The leptin receptor polymorphism seems to affect neuroendocrine regulation of energy balance among adolescents with obesity.
Embargo
Neuropeptidases in psychiatric disorders
(Elsevier, 2021) Nani, João Victor; Almeida, Priscila G. C.; Hayashi, Mirian [UNIFESP]; http://lattes.cnpq.br/5559309395232147; http://lattes.cnpq.br/1198445675076270; http://lattes.cnpq.br/2938015553073591
Psychiatric disorders (PDs), such as schizophrenia (SCZ), bipolar disorder (BD), and major depression disorder (MDD) are characterized by disturbances in the functioning of the CNS. These mental disorders have attracted the attention of researchers from different areas due to their high prevalence and extremely debilitating clinical characteristics. In spite of the many similarities observed among the PDs, not only in symptoms but also in the etiology as well, there is still a lack of knowledge in the biochemical and/or molecular pathways underlying each of these pathologies, in which better understanding could potentially help improve the diagnosis and possibilities for treatment. Several groups, including ours, have demonstrated that neuropeptidases and neuropeptidergic systems could play a fundamental role for the susceptibility of PDs such as SCZ, due to their involvement in the neurodevelopmental process (and consequent brain formation) and their recent association to disease progression. In this article, we bring an update of the main findings on neuropeptidases, such as Nuclear distribution element like-1 (NDEL1), angiotensin I-converting enzyme (ACE), and prolyl oligopeptidase (POP), with a detailed discussion on how they could be involved in the etiology of PDs. The main findings in the literature regarding alterations in neuropeptidase activity in different biological samples of patients with PDs, as well as in animal models, are presented here in order to draw the attention of general readers to the possibility of targeting this system for the discovery of new targets and/or development of novel therapies and gaining a deeper understanding of the molecular mechanisms underlying complex diseases such as PDs.
Acesso aberto (Open Access)
Neuropeptides and oligopeptidases in schizophrenia
(Pergamon Press, 2020-01) Rodriguez, Benjamín [UNIFESP]; Nani, João Victor [UNIFESP]; Almeida, Priscila G. C. [UNIFESP]; Brietzke, Elisa; Lee, Richard S.; Hayashi, Mirian A. F. [UNIFESP]; http://lattes.cnpq.br/5559309395232147
Schizophrenia (SCZ) is a complex psychiatric disorder with severe impact on patient's livelihood. In the last years, the importance of neuropeptides in SCZ and other CNS disorders has been recognized, mainly due to their ability to modulate the signaling of classical monoaminergic neurotransmitters as dopamine. In addition, a class of enzymes coined as oligopeptidases are able to cleave several of these neuropeptides, and their potential implication in SCZ was also demonstrated. Interestingly, these enzymes are able to play roles as modulators of neuropeptidergic systems, and they were also implicated in neurogenesis, neurite outgrowth, neuron migration, and therefore, in neurodevelopment and brain formation. Altered activity of oligopeptidases in SCZ was described only more recently, suggesting their possible utility as biomarkers for mental disorders diagnosis or treatment response. We provide here an updated and comprehensive review on neuropeptides and oligopeptidases involved in mental disorders, aiming to attract the attention of physicians to the potential of targeting this system for improving the therapy and for understanding the neurobiology underlying mental disorders as SCZ.
Acesso aberto (Open Access)
Reaction time and replenishment time of SP and CGRP after incision in rat skin
(De Gruyter Open Ltd, 2014-12-01) Hochman, Bernardo [UNIFESP]; Isoldi, Felipe Contoli [UNIFESP]; Freitas, Jose Octavio Gonçalves de [UNIFESP]; Lapin, Guilherme Abbud Franco [UNIFESP]; Espírito Santo, Paulo Rogério Quieregatto do [UNIFESP]; Silva, Erica Calcagno Raymundo da [UNIFESP]; Chadi, Gerson; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
Background. the skin neurogenic inflammation is mainly related to Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). There is no data on their availability in the dynamics of skin nerve endings, concerning their release and replenishment after a nociceptive stimulus, so this was investigated. Materials and methods. 25 rats were randomly distributed in 5 groups. the animals of the control group (CG) determined the baseline levels of neuropeptides in the skin. the groups S0 and S30 did not receive any cutaneous stimulus at 30 and 60 minutes, respectively. in the group S1, an incision stimulus was made at 30 minutes. in the group S31, a nociceptive stimulus was performed by subdermal scratching at 30 minutes and, at 60 minutes, the incision stimulus was carried out in the same location (nociceptive hyperstimulation). the skin samples of the other animals were harvested from the back 1 minute after their death. SP, pro-CGRP and CGRP were quantified by Western Blotting. Results. the incision stimulus released SP, S1 compared to S0 (p < 0.05) detected in the first minute, and the replenishment time was more than 30 minutes. Also, it cleaved pro-CGRP, S1 compared to S31 (p < 0.05) in the first minute, and its replenishment time less than 30 minutes. Release of CGRP was not detected. Conclusion. the incision released SP already detected in the first minute; its replenishment time is more than 30 minutes. the incision decreased pro-CGRP, also detected in the first minute; and its replenishment time is less than 30 minutes.
Somente Metadadados
A Research Design for the Quantification of the Neuropeptides Substance P and Calcitonin Gene-Related Peptide in Rat Skin Using Western Blot Analysis
(Lippincott Williams & Wilkins, 2015-06-01) Lapin, Guilherme Abbud Franco [UNIFESP]; Hochman, Bernardo [UNIFESP]; Nishioka, Michele Akemi [UNIFESP]; Maximino, Jessica Ruivo [UNIFESP]; Chadi, Gerson [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); IB CNPq Brazilian Natl Council Sci & Technol Dev
OBJECTIVE: To describe and standardize a protocol that overcomes the technical limitations of Western blot (WB) analysis in the quantification of the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) following nociceptive stimuli in rat skin.DESIGN: Male Wistar rats (Rattus norvegicus albinus) weighing 250 to 350 g were used in this study. Elements of WB analysis were adapted by using specific manipulation of samples, repeated cycles of freezing and thawing, more thorough maceration, and a more potent homogenizer; increasing lytic reagents; promoting greater inhibition of protease activity; and using polyvinylidene fluoride membranes as transfer means for skin-specific protein. Other changes were also made to adapt the WB analysis to a rat model.SETTING: University research center.MAIN OUTCOME MEASURE: Western blot analysis adapted to a rat model.RESULTS: This research design has proven effective in collecting and preparing skin samples to quantify SP and CGRP using WB analysis in rat skin.CONCLUSION: This study described a research design that uses WB analysis as a reproducible, technically accessible, and cost-effective method for the quantification of SP and CGRP in rat skin that overcomes technical biases.
Somente Metadadados
The role of nutritional profile in the orexigenic neuropeptide secretion in nonalcoholic fatty liver disease obese adolescents
(Lippincott Williams & Wilkins, 2010-05-01) Piano, Aline de [UNIFESP]; Tock, Lian [UNIFESP]; Carnier, June [UNIFESP]; Foschini, Denis [UNIFESP]; Sanches, Priscila de Lima [UNIFESP]; Correa, Fabiola Alvise [UNIFESP]; Oyama, Lila Missae [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Lederman, Henrique Manoel [UNIFESP]; Ernandes, Regina [UNIFESP]; Mello, Marco Tulio de [UNIFESP]; Tufik, Sergio [UNIFESP]; Dâmaso, Ana Raimunda [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
Background Little progress has been made to identify the central neuroendocrine pathway involved in the energy intake control in nonalcoholic fatty liver disease (NAFLD) patients.Objective To assess the influence of orexigenic neuropeptides in the nutritional aspects of NAFLD obese adolescents submitted to a long-term interdisciplinary approach.Methods Fifty adolescents aged 15-19 years, with body mass index at least 95th percentile, consisting of 25 patients without NAFLD and 25 with NAFLD. the NAFLD diagnosis was determined by ultrasonography. Blood samples were collected to analyze glycemia, hepatic transaminases, and lipid profile. Insulin resistance was estimated by Homeostasis Model Assessment Insulin Resistance Index. Neuropeptide Y (NPY) and agouti related protein concentrations were measured by enzyme-linked immunosorbent assay. Analyses of food intake were made by 3 days recordatory inquiry.Results At baseline conditions, the patients with NAFLD had significantly higher values of body mass, body mass index, visceral fat, triglycerides, VLDL-C, and hepatic transaminases. After the long-term intervention, they presented a significant reduction in these parameters. in both the groups, it was observed a significant decrease in energy intake, macronutrients and dietetic cholesterol. Only the patients with NAFLD presented a positive correlation between the saturated fatty acids intake and the orexigenic neuropeptides NPY and agouti related protein, and carbohydrate with NPY. Indeed, it was observed a positive correlation between energy intake, lipid (%) and saturated fatty acids with visceral fat accumulation.Conclusion Our findings showed an important influence of diet composition in the orexigenic system, being essential consider that the excessive saturated fatty acids intake could be a determinant factor to increase nonalcoholic fatty liver disease. Eur J Gastroenterol Hepatol 22:557-563 (C) 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Acesso aberto (Open Access)
Subchronical treatment with Fluoxetine modifies the activity of the MCHergic and hypocretinergic systems. Evidences from peptide CSF concentration and gene expression
(Elsevier Science Bv, 2016) Calegare, Bruno F. [UNIFESP]; Costa, Alicia; Fernandes, Leandro [UNIFESP]; Dias, Ana L. [UNIFESP]; Torterolo, Pablo; D'Almeida, Vania [UNIFESP]
In the postero-lateral hypothalamus are located two neuronal systems that utilize the neuropeptides melanin-concentrating hormone (MCH) and hypocretins (also called orexins) as neuromodulators. These systems have reciprocal connections between them, and project throughout the central nervous system. MCH has been involved in the generation of sleep, mainly REM sleep, while hypocretins have a critical role in the generation of wakefulness. MCHergic activity is also involved in the pathophysiology of major depressive disorder (MD). In this regards, intracerebral administration of MCH promotes pro-depressive behaviors (i.e., immobility in the forced swimming test) and REM sleep hypersomnia, which is an important trait of depression. Furthermore, the antagonism of the MCHR-1 receptor has a reliable antidepressant effect, suggesting that MCH is a pro-depressive factor. Hypocretins have been also involved in mood regulation; however, their role in depression is still on debate. Taking these data into account, we explored whether systemic subchronical treatment with Fluoxetine (FLX), a serotonergic antidepressant, modifies the concentration of MCH in the cerebrospinal fluid (CSF), as well as the preproMCH mRNA expression. We also evaluated the hypocretinergic system by quantifying the hypocretin-levels in the CSF and the preprohypocretin mRNA expression. Compared to control, FLX increased the levels of preprohypocretin mRNA without affecting the hypocretin-1 CSF levels. On the contrary, FLX significantly decreased the MCH CSF concentration without affecting the preproMCH gene expression. This result is in agreement with the fact that MCH serum level diminishes during the antidepressant treatment in MD, and supports the hypothesis that an increase in the MCHergic activity could have pro-depressive consequences. (C) 2016 Brazilian Association of Sleep. Production and Hosting by Elsevier B.V.

Navegando por Palavras-chave "Neuropeptides"

Resultados por página

Opções de Ordenação