Navegando por Palavras-chave "Mycobacterium leprae"

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    Aspectos Imunológicos da co-infecção do Mycobacterium leprae e o vírus da imunodeficiência humana
    (Universidade Federal de São Paulo (UNIFESP), 2008-08-27) Carvalho, Karina Inacio [UNIFESP]; Kallas, Esper Georges [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The immune response characteristics in patients infected with Mycobacterium leprae and human immunodeficiency virus (HIV) is not elucidated. The aim of this study was to evaluate different immune parameters in the overlapping of both diseases. In the first paper we observaded The co-infected group exhibited lower CD4:CD8 ratio, higher levels of CD8 T-cell activation, increased V1: V2 T cell ratio and lower percentage of plasmacytoid dendritic cells, compared to HIV-1 infected subjects. Across infected groups, IL-4 production by CD4 T lymphocytes was positively correlated with the percentage of effector memory CD4 T cells, suggesting antigenically-driven differentiation of such T cell population in both HIV-1 and M. leprae infections. Co-infection with M. leprae may exacerbate the immunopathology of HIV-1 induced disease. A T helper 2 (Th2) bias in the CD4 T-cell response was evident in both HIV-1-infection and leprosy, but no additive effect was apparent in co-infected patients. Subsequently, we evaluated quantitatively and qualitatively the NKT cells from innate immune response in HIV-infected subjects and healthy controls. The frequencies of NKT cells secreting IFN- and TNF- were significantly lower in HIV-1-infected subjects and the magnitude of the IFN- production was negatively correlated with the number of years of infection, suggesting that NKT cell function is progressively lost over time. NKT cell responses in HIV-1 infected subjects were essentially normal after treatment with Phorbol12-Myristate13- Acetato (PMA) and ionomycin, suggesting that defective TCR-signaling was the underlying defect in the cytokine production. The lower levels of the NKT Th1 response correlated with higher CD161 expression, suggesting a role for this inhibitory receptor in regulating NKT cell responsiveness. Finally, we have investigated the NKT cells in the context of HIV and M. leprae coinfection. The volunteers were enrolled into four groups: twenty-seven healthy controls, seventeen HIV seropositive patients, seventeen patients with leprosy, and twenty-three co-infected patients with leprosy and HIV-1 infection. Flow cytometric and ELISPOT assays were performed in stored PBMC. We demonstrated that coinfected patients have reduced NKT cells in the peripheral blood when compared to healthy subjects and leprosy monoinfected patients. On the other hand, NKT cells from coinfected patients secrete more IFN- when compared to leprosy monoinfected patients. These results suggest that NKT cells are highly active in coinfected patients, although occurring in lower frequency in the peripheral blood.
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    Auto-anticorpos em pacientes com hanseníase com e sem comprometimento articular, no estado do Amazonas
    (Universidade Federal de São Paulo (UNIFESP), 2009-08-26) Ribeiro, Sandra Lúcia Euzébio [UNIFESP]; Sato, Emilia Inoue [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objetivos: Determinar a frequencia de fator reumatoide (FR IgM) e anticorpos antipeptideos citrulinados ciclicos (anti-CCP), antinucleares (AANs), anticitoplasma de neutrofilos (ANCA), anticardiolipina (aCL) e anti-ƒÀ2glicoproteina ƒ§ (anti-ƒÀ2GPƒ§) em pacientes com hanseniase com e sem comprometimento articular, avaliando uma possivel associacao entre estes auto-anticorpos e comprometimento articular, forma clinica, reacao hansenica, alta e tratamento com poliquimioterapia (PQT). Pacientes e Metodos: Foram incluidos 158 pacientes com hanseniase em acompanhamento no Ambulatorio de Dermatologia e Venereologia Fundacao gAlfredo da Matta h em Manaus (AM), no periodo de 06/2004 a 10/2006, sendo 76 com (Grupo ƒ§) e 82 sem comprometimento articular (Grupo ƒ§ƒ§) comparaveis quanto a idade, sexo, forma clinica, reacao e tratamento. O grupo ƒ§ƒ§ƒ§ foi constituido por 129 individuos sadios residentes em Manaus, com distribuicao de sexo e faixa etaria semelhante a dos pacientes. Para a pesquisa dos auto-anticorpos foram utilizadas as tecnicas: aglutinacao com particulas de latex para pesquisa de FR IgM, imunofluorescencia indireta para AANs e ANCA e ELISA para anti-CCP, aCL e anti-ƒÀ2GPƒ§. Resultados: A media de idade do grupo ƒ§ foi 39,8 }15,8 anos e do grupo ƒ§ƒ§ 40,6 }14,8 (p=0,716); 51 pacientes (67,1%) do grupo ƒ§ e 62 (75,6%) do grupo ƒ§ƒ§ eram do sexo masculino (p=0,237). Dentre os 76 pacientes do Grupo ƒ§, 61 tinham artrite e 15, artralgia. Dentre 158 pacientes com hanseniase, 56 (35,4%) apresentavam a forma virchowiana (VV). Episodios reacionais foram igualmente observados nos dois grupos, estando presentes em 24 (31,6%) pacientes do Grupo ƒ§ e em 34 (41,5%) do Grupo ƒ§ƒ§ (p=0,198). A frequencia de anticorpos anti-CCP, FR e AANs nos Grupos ƒ§ e ƒ§ƒ§ foi baixa e semelhante a do Grupo ƒ§ƒ§ƒ§. ANCA nao foi detectado em nenhum dos Grupos. Anticorpos aCL foram mais frequentes nos pacientes com hanseniase (Grupos ƒ§ e ƒ§ƒ§) que em controles sadios (15,8% vs 3,1%; p<0,001), nao sendo observada diferenca entre os Grupos ƒ§ e ƒ§ƒ§ (17,1% vs 14,6%, p=0,671). Anticorpos anti-ƒÀ2GPƒ§ tambem foram mais frequentes em pacientes que em controles (46,2% vs 9,4; p<0,001), sem diferenca significante entre os Grupos ƒ§ e ƒ§ƒ§ (43,4% vs 48,8%, p=0,500). Houve predominio do isotipo IgM em relacao ao IgG tanto para aCL (88% vs 16%, p=0,001) quanto para anti-ƒÀ2GPƒ§ (97,3% vs 12,3%, p<0,001). Nenhum paciente apresentou manifestacoes sugestivas de trombose vascular. Conclusao: A frequencia de anticorpos aCL e anti-ƒÀ2GPƒ§ foi significativamente maior em pacientes com hanseniase que em controles sadios, o que nao foi observada nos demais auto-anticorpos estudados. Nao foi observada associacao entre positividade para os auto-anticorpos estudados e comprometimento articular, presenca de episodios reacionais, alta ou tratamento com PQT, nem com a forma clinica da hanseniase, exceto para anticorpos aCL que foram mais frequentes na forma clinica VV. Embora tenha sido encontrada alta positividade para anticorpos antifosfolipides dependentes de ƒÀ2GPƒ§ nos pacientes com hanseniase, nao houve associacao com fenomenos tromboticos, mostrando que, nestes pacientes, a dependencia a ƒÀ2GPƒ§ nao esta associada a patogenicidade atribuida a estes anticorpos
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    Auto-anticorpos em pacientes com hanseniase com e sem comprometimento articular, no estado do Amazonas
    (Universidade Federal de São Paulo (UNIFESP), 2009) Ribeiro, Sandra Lucia Euzebio [UNIFESP]
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    Avaliação e monitoramento dos contatos intradomiciliares de pacientes com hanseníase: sorologia para detecção de anticorpos anti-PGL-I e avaliação da reatividade a proteínas recombinantes do Mycobacterium leprae
    (Universidade Federal de São Paulo (UNIFESP), 2016-04-27) Silva, Eliane Aparecida [UNIFESP]; Tomimori, Jane [UNIFESP]; http://lattes.cnpq.br/1123390691453566; http://lattes.cnpq.br/1025452284973109; Universidade Federal de São Paulo (UNIFESP)
    Leprosy, a chronic disease with high infectivity and low pathogenicity, manifests by dermato-neurological signs and symptoms and may cause physical disabilities and deformities. The leprosy elimination strategy is based on case detection in the early stages of the disease and the effectiveness of chemotherapy. In order to assist in the diagnosis of the disease, serological tests have been developed. Thus, this study aims to: I. to validate the utility of fusion antigen LID-1 associated with the synthetic disaccharide (ND-O) of phenolic glycolipid-I (PGL-I), in field work; II. to identify the number of leprosy cases diagnosed in Rondonópolis-MT during five years of followup of household contacts at the time of diagnosis of the index case, using clinical and serological exams (IgM anti ND-O-BSA and IgM/IgG anti ND-O-LID-1). In the period from 2009 to 2010, in Rondonopolis-MT, active search for new leprosy cases and their household contacts was carried out. From this work, in the present study we evaluated serum samples, clinical and epidemiological data of diagnosed leprosy cases classified by bacteriological, histological and clinical criteria (n = 174); household contacts who lived with the index cases at diagnosis or in the last five years (n = 409); individuals from endemic areas without diagnosis and family history of leprosy (n = 53); patients diagnosed with pulmonary tuberculosis (n = 12). Household contacts were followed (n = 186) over a five-year period. Blood was collection by venipuncture for serological tests at diagnosis and during follow-up of five years, in addition, dermatological and neurological examination were performed. For enzyme immunoassay for the detection of anti-PGL-I antibody (ND-O-BSA) were considered positive OD ? 0.150 and for the anti-ND-O-LID-1 immunodot positivity was given in crosses (0,5+, 1+, 2+, 3+). Results of antibodies serum levels stratified by clinical form (43 tuberculoid, 63 borderline-tuberculoid, 30 borderline-borderline, 12 borderline-lepromatous, 07 lepromatous, 19 indeterminate) showed higher positivity for the rapid testing in MB patients: 100% for VV and DV, and 73.33% for DD. Individuals PB showed 7.20% positivity to the rapid test. The positivity of anti-PGL-I ELISA among 409 contacts was low (3.17%) and for the anti-ND-O-LID-1 rapid test was 14.66%, with nine positive samples 1+ and 51 positive 0,5+. In the first evaluation, nine (2.20%) contacts were diagnosed with leprosy, six were negative for both serological tests, two 0,5+ for LID-1 and negative for PGL-I and one 1+ to LID -1 and positive to PGL-I. When comparing all patients r2 = 0.8444 correlation was obtained indicating good agreement between the serological tests. Correlation r2 = 0.7382 was found between results of ND-O-LID-1 and bacterial index of MB patients.The results showed that levels of anti-ND-O-BSA antibodies by ELISA correspond to semi-quantitative results of anti-ND-O-LID-1 in the rapid test. Clinical examination of household contacts proved to be a good procedure for active case finding, as it detected new leprosy cases in 2.2% of examined contacts. The followup of household contacts for five years made possible the detection of 3.22% of new cases among contacts. The quantification of anti-ND-O-BSA by ELISA can be used as a laboratory tool to indicate exposure to M. leprae in contacts. Positive serology may indicate infection with M. leprae, without necessarily evolution to disease. All these observations allowed us to conclude that individuals with positive serology should be followed for longer periods than recommended by the control program.
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    Caracterização da atividade proteolítica da Hsp65 de Mycobacterium leprae
    (Universidade Federal de São Paulo (UNIFESP), 2002) Arauz, Luciana Juncioni de [UNIFESP]; Camargo, Antonio Carlos Martins de [UNIFESP]
    O presente trabalho relata, pela primeira vez, que a hsp65 recombinante de Mycobacterium leprae (chaperonina 2) apresenta atividade proteolitica. A atividade proteolitica da hsp65 de M. leprae mostrou uma especificidade semelhante a tripsina perante peptideos de fluorescencia apagada derivados da dinorfina. Quando outros substratos peptidicos foram utilizados b-endorfina, neurotensina e angiotensina I) a clivagem da ligacao peptidica predominante tambem envolveu aminoacidos basicos em P1 embora, em menor extensao, tambem foi observada a hidrolise envolvendo aminoacidos neutros e hidrofobicos (G e F). O alinhamento da sequencia de aminoacidos da hsp65 de M. leprae com a protease HSIW de Escherichia coli sugere dois grupos cataliticos putativos de treonina, um no dominio-N (T136, K168 e Y264) e o outro no dominio-C (T375, K4º9 e S$º2). Estudos de mutagenese mostraram que a substituicao de K409 por A causou uma perda completa da atividade proteolitica, ao passo que a mutacao de K168 para A resultou em uma perda de 25 por cento. Estes resultados sugerem fortemente que os residuos de aminoacidos T3'5, K 4o9 e S'º' no dominio-C formem o grupo catalitico responsavel pela atividade proteolitica da hsp65 de M. leprae. As possiveis implicacoes patofisiologicas da atividade proteolitica da hsp65 de M. leprae estao agora sob ampla investigacao em nosso laboratorio
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    Caracterização da especificidade primária da hsp65r de Mycobacterium leprae e estudo da possível relação desta atividade catalítica com o seu poder imunogênico
    (Universidade Federal de São Paulo (UNIFESP), 2003) Marengo, Eliana Blini [UNIFESP]; Camargo, Antonio Carlos Martins de [UNIFESP]
    Neste trabalho, o possivel envolvimento da atividade proteolitica da hsp65 chaperonina 2 (cpn2) de M. leprae na apresentacao de epitopos MHC classe 1 esta sendo sugerido. O estudo da especificidade primaria da hsp65 recombinante cpn 2 de Mycobacterium leprae frente a substratos peptidicos fluorescentes, mostrou especificidade por residuos basicos em posicao P,, em adicao a atividade tripsina-simile, fato que ja foi relatado pelo nosso grupo (Portaro e cols, 2002). Observou-se hidrolise de substratos de tamanho restrito, entre 7 e 13 residuos de aminoacidos e, em menor proporcao, clivagem da ligacao peptidica no substrato analogo ao receptor de trombina PARI envolvendo aminoacido hidrofobico (Phe) em PI. Durante o estudo da caracterizacao de sua atividade proteolitica, verificamos, pela primeira vez, que apresenta atividade ATPasica (Km 95 p.M e kcat = 2,6 min -1) e que sua atividade enzimatica e influenciada por nucleotideos e cations monovalentes e bivalentes. Esse trabalho, ainda, corelaciona as sequencias de aminoacidos, focalizando epitopos MHC classe 1, e as atividades adjuvantes e cataliticas de hsps65. As possiveis implicacoes fisiopatologicas da atividade proteolitica da hsp65 de M. leprae estao agora sob ampla investigacao em nosso laboratorio
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    Comparação entre as leituras clínica e histológica da reação de Mitsuda em indivíduos sadios adultos não comunicantes de hansenianos
    (Universidade Federal de São Paulo (UNIFESP), 1982) Petri, Valeria [UNIFESP]; Mendes, Ernesto Vieira [UNIFESP]
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    O efeito da administração da HSP65 de Mycobacterium leprae na progressão do lúpus eritematoso sistêmico murino
    (Universidade Federal de São Paulo (UNIFESP), 2008) Marengo, Eliana Blini [UNIFESP]; Sant'Anna, Osvaldo Augusto [UNIFESP]
    A família de proteínas de choque térmico de 60 kDa [Hsp60] e abundante em células de procariotos e eucariotos, altamente conservadas evolutivamente, e participam de diversas etapas nos processos de síntese proteíca. Além dessas características, expressam-se em altos níveis durante fenômenos auto-imunes e inflamatórios. Nesse estudo, 0 envolvimento fisiopatológico da Hsp65 recombinante nativa [Hsp65r WT] e do mutante pontual K409 A de M. leprae foi avaliado in vivo no modelo de Lupus Eritematoso Sistêmico [LES] em camundongos geneticamente homogêneos [NZBxNZW]F1. As respostas de anticorpos anti-DNA e anti-Hsp65 foram individualmente dosadas ao longo da vida do animal e 0 tempo médio de sobrevida [TMS] foi determinado. 0 tratamento com a WT abrevia 0 TMS em 50% quando comparado aos não-tratados, e 0 aumento da razão de anticorpos IgG2a/lgG1 anti-DNA foi também observada nos animais inoculados com WT. A incubação de macrófagos de camundongos BALB/c com soro de F1 tratados com WT resultou em necrose celular imediata, enquanto 0 tratamento com 0 soro de F1 tratados com K409A nao causou qualquer efeito tóxico. Além disso, 0 envolvimento da WT está correlacionado com a idade e é dose-dependente. A administração combinada da Hsp65r WT e K409A mostrou que a K409A e capaz de inibir, mas não reverter 0 efeito da WT na morte dos híbridos F1. As ações semelhantes na sobrevida desses F1 entre os tratados com PEP LEADER e PEP K409A, e suas respectivas proteínas, sugerem 0 efeito patogênico da região 352-371 da Hsp65 no LES experimental. Nossos dados sugerem que a Hsp65 tem participação central na intervenção da progressao do LES, e que a molécula imunogênica de mutação pontual K409A, contrapõe 0 efeito deletério da WT, amenizando e retardando 0 desenvolvimento de LES nos tratados. Este estudo abre novas perspectivas para esclarecer 0 efeito biológico geral das Hsp e demonstra de modo claro, 0 impacto dos fatores ambientais durante a patogênese deste processo auto-imune..
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    Estudo das manifestacoes articulares relacionadas a hanseniase
    (Universidade Federal de São Paulo (UNIFESP), 2008) Pereira, Helena Lucia Alves [UNIFESP]
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    Leprosy-related joint involvement
    (Springer, 2009-01-01) Pereira, H. L. A. [UNIFESP]; Ribeiro, S. L. E. [UNIFESP]; Pennini, S. N.; Sato, E. I. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Fed Amazonas; Alfredo da Matta Fdn
    We estimate the prevalence and evaluate the clinical characteristics of leprosy related arthritis. One thousand, two hundred fifty-seven leprosy patients were attended at Alfredo da Matta outpatient clinic in the state of Amazonas, Brazil from July to October 2004. Among them, 115 patients were identified with articular pain and were referred for evaluation with rheumatologist. Blood samples were collected and radiological evaluation of the involved joints was performed. All patients with arthritis who continued to be followed up were reevaluated. One hundred fifteen leprosy patients (9.1%) were identified with articular involvement. the articular complaints were attributed to a defined rheumatic disease in 36 cases and excluded from further analysis. Twenty-four patients had arthralgia, and 55 (37 males and 18 females) had leprosy-related arthritis. the prevalence of arthritis was similar in both genders, and all patients with leprosy-related arthritis had lepromatous or borderline type. Most of patients had polyarticular and symmetrical arthritis and had completed the multidrug therapy and was under reaction treatment. the mean duration of articular symptoms at the time of study was 1.06 years (ranging from 5 days to 14 years). Ninety-one percent of patients with leprosy-related arthritis presented erythema nodosum leprosum or reversal reactions. Only five patients with arthritis had never presented reactions. Fifty percent of patients became asymptomatic during the mean 24 months of follow-up. Leprosy-related arthritis has a lower prevalence than previously reported. Most cases of leprosy-related arthritis were associated with reactional episodes, and in a large number of cases, the arthritis had a chronic course not responsive to the conventional therapy for reactions.
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    Lower numbers of natural killer T cells in HIV-1 and Mycobacterium leprae co-infected patients
    (Wiley-Blackwell, 2012-05-01) Carvalho, Karina I.; Bruno, Fernanda R.; Snyder-Cappione, Jennifer E.; Maeda, Solange M. [UNIFESP]; Tomimori, Jane [UNIFESP]; Xavier, Marilia B.; Haslett, Patrick A.; Nixon, Douglas F.; Kallas, Esper G. [UNIFESP]; Universidade de São Paulo (USP); Univ Calif San Francisco; Universidade Federal de São Paulo (UNIFESP); Fed Univ Para; Shire Human Genet Therapies
    Natural killer T (NKT) cells are a heterogeneous population of lymphocytes that recognize antigens presented by CD1d and have attracted attention because of their potential role linking innate and adaptive immune responses. Peripheral NKT cells display a memory-activated phenotype and can rapidly secrete large amounts of pro-inflammatory cytokines upon antigenic activation. in this study, we evaluated NKT cells in the context of patients co-infected with HIV-1 and Mycobacterium leprae. the volunteers were enrolled into four groups: 22 healthy controls, 23 HIV-1-infected patients, 20 patients with leprosy and 17 patients with leprosy and HIV-1-infection. Flow cytometry and ELISPOT assays were performed on peripheral blood mononuclear cells. We demonstrated that patients co-infected with HIV-1 and M.leprae have significantly lower NKT cell frequencies [median 0.022%, interquartile range (IQR): 0.0070.051] in the peripheral blood when compared with healthy subjects (median 0.077%, IQR: 0.0320.405, P < 0.01) or HIV-1 mono-infected patients (median 0.072%, IQR: 0.0300.160, P < 0.05). Also, more NKT cells from co-infected patients secreted interferon-? after stimulation with DimerX, when compared with leprosy mono-infected patients (P = 0.05). These results suggest that NKT cells are decreased in frequency in HIV-1 and M.leprae co-infected patients compared with HIV-1 mono-infected patients alone, but are at a more activated state. Innate immunity in human subjects is strongly influenced by their spectrum of chronic infections, and in HIV-1-infected subjects, a concurrent mycobacterial infection probably hyper-activates and lowers circulating NKT cell numbers.
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    Mycobacterium leprae is identified in the oral mucosa from paucibacillary and multibacillary leprosy patients
    (Wiley-Blackwell, 2014-01-01) Abreu, Marilda Aparecida Milanez Morgado de [UNIFESP]; Roselino, A. M.; Enokihara, M. [UNIFESP]; Nonogaki, S.; Prestes-Carneiro, L. E.; Weckx, Luc Louis Maurice [UNIFESP]; Alchorne, Maurício Mota de Avelar [UNIFESP]; Univ Oeste Paulista; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Adolfo Lutz Inst
    In leprosy, the nasal mucosa is considered as the principal route of transmission for the bacillus Mycobacterium leprae. the objective of this study was to identify M.leprae in the oral mucosa of 50 untreated leprosy patients, including 21 paucibacillary (PB) and 29 multibacillary (MB) patients, using immunohistochemistry (IHC), with antibodies against bacillus Calmette-Guerin (BCG) and phenolic glycolipid antigen-1 (PGL-1), and polymerase chain reaction (PCR), with MntH-specific primers for M.leprae, and to compare the results. the material was represented by 163 paraffin blocks containing biopsy samples obtained from clinically normal sites (including the tongue, buccal mucosa and soft palate) and visible lesions anywhere in the oral mucosa. All patients and 158 available samples were included for IHC study. Among the 161 available samples for PCR, 110 had viable DNA. There was viable DNA in at least one area of the oral mucosa for 47 patients. M.leprae was detected in 70% and 78% of patients using IHC and PCR, respectively, and in 94% of the patients by at least one of the two diagnostic methods. There were no differences in detection of M.leprae between MB and PB patients. Similar results were obtained using anti-BCG and anti-PGL-1 antibodies, and immunoreactivity occurred predominantly on free-living bacteria on the epithelial surface, with a predilection for the tongue. Conversely, there was no area of predilection according to the PCR results. M.leprae is present in the oral mucosa at a high frequency, implicating this site as a potential means of leprosy transmission.
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    Pesquisa de anticorpos anti PGL-I através de ELISA em tatus selvagens do Brasil
    (Sociedade Brasileira de Medicina Tropical - SBMT, 2008-01-01) Deps, Patrícia Duarte [UNIFESP]; Antunes, João Marcelo Azevedo de Paula; Faria, Carlos; Bührer-Sékula, Samira; Camargo, Zoilo Pires de [UNIFESP]; Opromola, Diltor Vladimir Araujo; Tomimori, Jane [UNIFESP]; Universidade Federal do Espírito Santo Departmento de Medicina Social; London School of Hygiene & Tropical Medicine Departmento de Infectologia e Doenças Tropicais; Escola de Medicina da Santa Casa de Misericórdia Departmento de Patologia; Royal Tropical Institute; Universidade Federal de Goiás Instituto de Patologia Tropical e Saúde Pública; Universidade Federal de São Paulo (UNIFESP); Instituto Lauro de Souza Lima
    Armadillos have been involved in leprosy transmission and are considered a source of Mycobacterium leprae in numerous reports. Clinicians from certain areas of the USA consider contact with armadillos a risk factor for leprosy. However, there is a challenge associated with the role of wild armadillos perpetuating human leprosy in the American Continent. The presence of anti-PGL-I antibodies was investigated in wild nine-banded armadillos from leprosy-endemic areas in State of Espirito Santo, Brazil, by ELISA performed on serum samples from 47 armadillos. Positive ELISA was obtained from 5 (10.6%) armadillos. Infected armadillos may play some role in leprosy transmission, disseminating bacilli in the environment, perhaps making it more difficult to interrupt transmission and reduce the number of new leprosy cases. ELISA is an efficient tool for seroepidemiological investigations of Mycobacterium leprae in armadillos.
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    Reação de mitsuda em contatos consanguíneos e não consanguíneos de doentes de hanseníase com formas bacilíferas
    (Universidade Federal de São Paulo (UNIFESP), 1987) Trindade, Maria Angela Bianconcini [UNIFESP]; Petri, Valeria [UNIFESP]
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    Receptores de membrana de linfocitos em pacientes com hanseniase
    (Universidade Federal de São Paulo (UNIFESP), 1976) Saraiva, Paulo Jaconi [UNIFESP]
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    Serum anti-phenolic glycolipid1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?
    (Wiley, 2018) Macedo, Alexandre C. de; Guimaraes, Juliana A.; Rodrigues, Raphael O.; Araujo, Thiago D. V.; Tavares, Clodis M.; Cabral, Paula B.; Moraes-Pinto, Maria Isabel de [UNIFESP]; Nagao-Dias, Aparecida T.
    ObjectiveThe aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. MethodAnalysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. ResultsA strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MB patients. A moderate correlation was found in all analyses in PB patients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%