Navegando por Palavras-chave "Mycobacterium chelonae"
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- ItemAcesso aberto (Open Access)Deep stromal mycobacterial keratitis: viable bacteria after six months of treatment: case report and literature review(Conselho Brasileiro de Oftalmologia, 2005-08-01) Gusmão, Filipe Brandão Accioly de [UNIFESP]; Alvarenga, Lênio [UNIFESP]; Barbosa, Luciene [UNIFESP]; Sampaio, Jorge; Leao, Sylvia Cardoso [UNIFESP]; Hofling-Lima, Ana Luisa [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Laboratório FleuryTo report the presence of viable mycobacteria in a patient with keratitis treated for 6 months. Species identification was performed using the PRA method (polymerase chain reaction followed by restriction endonuclease analysis). Clonality was evaluated with RAPD (randomly amplified polymorphic DNA) and ERIC-PCR (enterobacterial repetitive intergenic consensus - polymerase chain reaction) methods. The patient reported trauma due to a metallic foreign body 3 weeks prior to presentation. Initial corneal scraping cultures revealed Mycobacterium abscessus. After 6 months of topical and systemic treatment the patient presented with no active inflammation and was considered clinically cured. An optic penetrating keratoplasty was performed. Culture of the excised cornea revealed Mycobacterium abscessus. Both isolates had the same clonal origin. The most interesting finding of this case report was the positive culture of the excised cornea after 6 months of intensive specific topical therapy. To our knowledge, this is the first report in the literature showing this possibility in the treatment of Mycobacterial keratitis. Thus, Mycobacterium abscessus may present viable bacteria after long-term treatment and should be followed carefully for a long period of time after tapering the medication.
- ItemSomente MetadadadosDesenvolvimento de um modelo animal experimental de infecção por Mycobacterium chelonae em olhos submetidos a ceratotomia lamelar automatizada(Universidade Federal de São Paulo (UNIFESP), 2004) Adan, Consuelo Bueno Diniz [UNIFESP]; Freitas, Denise de [UNIFESP]Objetivo: Desenvolver um modelo experimental de ceratite infecciosa por Mycobacterium chelonae em olhos submetidos a ceratotomia lamelar estromal automatizada. Material e Metodo: Estudo experimental animal, prospectivo, mascarado. Foram feitas tres series de experimentos com quatro animais de estudo e quatro controles em cada serie. Os animais (coelhos albinos, machos, raca Nova Zelandia, com peso de 3,5 a 4,0 kg) foram anestesiados e sedados. Foram utilizados para ceratotomia, o microceratomo Automatic Corneal Shaper (ACS®) e inoculos com bacterias vivas ou inativadas pelo calor. Os olhos tiveram imunossupressao local e antibioticoterapia profilatica pos-operatoria. A observacao clinica e documentacao fotografica em lampada de fenda, em determinados periodos pos-operatorios (pos-operatorio imediato - POI, 3°, 5°, 7°, 11°, 16°, e 23° dias) foram feitas por dois examinadores. Nestes mesmos periodos foi tambem realizada a documentacao fotografica. Ao estabelecimento da doenca corneana (pela concordancia entre os examinadores), os animais foram sacrificados. As corneas foram trepanadas e maceradas para estudo microbiologico. Resultado: Verificou-se a recuperacao do agente em nove dos doze animais. Um animal da terceira serie foi a obito. Conclusao: Foi possivel a inducao de infeccao corneana por M. chelonae em olhos de coelhos submetidos a ceratotomia lamelar. Este modelo podera permitir o estudo da patogenese, bem como o diagnostico e tratamento da infeccao por micobacteria apos ceratomileusis in situ assistida por laser (LASIK)
- ItemSomente MetadadadosEnterobacterial repetitive intergenic consensus PCR is a useful tool for typing Mycobacterium chelonae and Mycobacterium abscessus isolates(Elsevier B.V., 2006-06-01) Sampaio, JLM; Viana-Niero, C.; De Freitas, D.; Hofling-Lima, A. L.; Leao, S. C.; Universidade Federal de São Paulo (UNIFESP); Inst Fleury Ensino & PesquisaOutbreaks of rapidly growing mycobacterium (RGM) infections are increasingly being reported worldwide. Information about genetic relatedness of isolates obtained during outbreaks can provide Opportunities for prompt intervention. Pulsed-field gel electrophoresis (PFGE) is expensive, time consuming, and labor intensive. Other than that, Mycobacterium abscessus isolates call suffer DNA degradation during electrophoresis. Polymerase chain reaction (PCR)-based methods are cheaper, faster, and easier to perform, but discriminatory power varies depending oil the primer used. Ill this study, we tested the competence of enterobacterial repetitive intergenic consensus (ERIC) PCR in comparison with PFGE to distinguish unrelated isolates (24 Mycobacterium chelonae and 24 M. abscessus) obtained front human and/or environmental samples and to group 56 isolates front 6 outbreaks confirmed epidemiologically, caused by M. chelonae and M abscessus after ophthalmologic refractive surgery and mesotherapy. Enterobacterial repetitive intergenic consensus PCR presented discriminatory power. calculated using Simpson's index of diversity, of 0.989 for M abscessus and 0.975 for M. chelonae and grouped outbreak isolates ill distinct groups showing epidemiologic concordance. Pulsed-field gel electrophoresis also grouped outbreak isolates and presented discriminatory power of 0.972 and 0.993 for M. abscessus and M. chelonae, respectively. DNA from 8 (22%) of 36 M. abscessus isolates analyzed showed degradation during electrophoresis. Compared with PFGE and epidemiologic information as the gold standard, ERIC PCR is a simple, high throughput, affordable, reproducible, and discriminatory molecular typing method for inference of genetic relatedness of RGMs of the M. chelonae-abscessus group. (c) 2006 Elsevier Inc. All rights reserved.
- ItemAcesso aberto (Open Access)An experimental model of mycobacterial infection under corneal flaps(Associação Brasileira de Divulgação Científica, 2004-07-01) Adan, Consuelo Bueno Diniz [UNIFESP]; Sato, Elcio Hideo [UNIFESP]; Sousa, Luciene Barbosa de [UNIFESP]; Oliveira, Rosangela Siqueira de [UNIFESP]; Leao, Sylvia Cardoso [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st, 3rd, 5th, 7th, 11th, 16th, and 23rd postoperative days. Slit lamp photographs were taken to document clinical signs. Animals were sacrificed when corneal disease was detected and corneal samples were taken for microbiological analysis. Eleven of 12 experimental rabbits developed corneal disease, and M. chelonae could be isolated from nine rabbits. Eleven of the 12 controls receiving a heat-inactivated inoculum did not develop corneal disease. M. chelonae was not isolated from any of the control rabbits receiving a heat-inactivated inoculum, or from the healthy cornea of control rabbits. Corneal infection by M. chelonae was successfully induced in rabbits submitted to keratomileusis. To our knowledge, this is the first animal model of M. chelonae infection following corneal flaps for refractive surgery to be described in the literature and can be used for the analysis of therapeutic responses.
- ItemSomente MetadadadosIn vitro activity of fluoroquinolones against Mycobacterium abscessus and Mycobacterium chelonae causing infectious keratitis after LASIK in Brazil(Lippincott Williams & Wilkins, 2005-08-01) Hofling-Lima, Ana Luisa [UNIFESP]; Freitas, Denise de [UNIFESP]; Sampaio, Jorge Luiz Mello [UNIFESP]; Leao, Sylvia Cardoso [UNIFESP]; Contarini, Patricia [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Purpose: To evaluate the in vitro activity of fluoroquinolones against Mycobacterium abscessus and Mycobacterium chelonae isolated from outbreaks of infectious keratitis in Brazil.Material and Methods: Micobacterial isolates were recovered from infectious keratitis cases related outbreaks that occurred in Brazil after LASIK for myopia. Two outbreaks occurred in Rio de Janeiro in 1998 and 1999, and 3 in Sao Paulo between 2000 and 2003. All laboratorial analysis, including molecular identification and antibiotic susceptibility testing with determination of the minimum inhibitory concentration (MIC) levels for ciprofloxacin, ofloxacin, gatifloxacin, and moxifloxacin, were performed at Universidade Federal de Sao Paulo in Brazil.Results: Fifteen samples were identified as M chelonae, and 3 were identified as M. abscessus. The outbreaks studied were designated SP-1 in 2000; SP-2 in 2000-2001; and SP-3 in 2003, R1 in 1988 and R2 in 1999. All but 1 of the M. chelonae were resistant to all fluoroquinolones with an MIC90 greater than 32 mu g/mL. The only susceptible isolate had MIC levels for ciprofloxacin, ofloxacin, gatifloxacin, and moxifloxacin of 0.38 mu g/mL, 0.032 mu g/mL, 0.047 mu g/mL, and 0.19 mu g/mL, respectively. MIC levels for all 3 M abscessus isolates tested were greater then 32 mu g/mL for all fluoroquinolones tested.Conclusions: Fluoroquinolone MICs for 17 M abscessus and M chelonae isolates recovered from infectious keratitis cases in Brazil indicate that they are not susceptible to these drugs in vitro. Further studies to investigate the in vivo effectiveness of fluoroquinolones against mycobacteria are required because in vitro tests do not support their use in the treatment of micobacterial keratitis in this particular geographic area.
- ItemSomente MetadadadosMycobacterium chelonae valve endocarditis resulting from contaminated biological prostheses(W B Saunders Co Ltd, 2010-06-01) Strabelli, T. M. V.; Siciliano, Rinaldo Focaccia; Castelli, Jussara Bianchi; Demarchi, L. M. M. F.; Leao, Sylvia Cardoso [UNIFESP]; Viana-Niero, Cristina [UNIFESP]; Miyashiro, Kozue; Sampaio, Roney Orismar; Grinberg, Max; Uip, David Everson; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Inst FleuryObjectives: A rapid-growing mycobacteria biological prosthetic valve (BPV) endocarditis related to prosthetic manufacturing process is described in Brazil.Methods: From 1999 to 2008, thirty-nine patients underwent BPV replacement due to culture-negative suspected endocarditis. All these cases had histological sections stained by Ziehl-Neelsen method. Clinical and microbiological data were reviewed in all acid-fast bacilli (AFB) positive cases. the 16S-23S internal transcribed sequence (ITS) was amplified using DNA extracted from paraffin-embedded samples, digested with restrictions enzymes and/or sequenced.Results: Eighteen AFB positive BPV (18/39)(46%) were implanted in 13 patients and were from the same manufacturer. Four of them were implanted in other hospitals. Thirteen BPV were histologically proven endocarditis and five showed a colonization pattern. the examination of six non-implanted sterile BPV from this manufacturer resulted in 5 AFB positive. Mycobacterium chelonae was the AFB identified by ITS restriction analysis and sequencing.Conclusions: Rapid-growing mycobacteria infections must be suspected and Ziehl-Neelsen stain always performed on histology of either early or late BPV endocarditis, particularly when blood cultures are negative. (C) 2010 the British Infection Society. Published by Elsevier B.V. All rights reserved.
- ItemAcesso aberto (Open Access)Ocorrência de tuberculose em um hospital psiquiátrico do interior de Goiás(Sociedade Brasileira de Pneumologia e Tisiologia, 2006-12-01) Costa, Hindenburg Cruvinel Guimarães Da; Malaspina, Ana Carolina; Mello, Fernando Augusto Fiúza De [UNIFESP]; Leite, Clarice Queico Fujimura; Universidade Estadual Paulista (UNESP); Universidade Federal de São Paulo (UNIFESP); Instituto Clemente FerreiraOBJECTIVE: To investigate the prevalence of infection, disease and eventual institutional outbreak of tuberculosis in a psychiatric hospital using the PPD test, as well as testing for mycobacteria in material collected from the respiratory tree and using molecular tracking technique based on insertion sequence 6110 (IS6110). METHODS: Between February and August of 2002, PPD tests were given to 74 inpatients and 31 staff members at a psychiatric hospital in the city of Rio Verde, located in the state of Goiás, Brazil. In addition, respiratory tree material collected from the inpatients was submitted to testing for Mycobacterium tuberculosis. RESULTS: Among the patients analyzed, mycobacteria were isolated from five (6.8%): four identified as M. tuberculosis and one as M. chelonae. The M. tuberculosis isolates were sensitive to isoniazid and rifampicin, and, when submitted to the restriction fragment length polymorphism/IS6110 technique, presented unique genetic profiles, totally distinct from one another, suggesting that all of the tuberculosis cases were due to endogenous reactivation. It was not possible to characterize this group of cases as an institutional outbreak. Performing the two-step tuberculin test in the patients, the infection rates were 23% and 31%, compared with 42% among staff members, who were submitted to the one-step test. CONCLUSION: The results indicate a high incidence of tuberculosis infection among inpatients and hospital staff, as well as a high occurrence of the disease among inpatients.
- ItemSomente MetadadadosPerformance de tres metodos na avaliacao da similaridade genetica de isolados do grupo Mycobacterium chelonae-abscessus(Universidade Federal de São Paulo (UNIFESP), 2005) Sampaio, Jorge Luiz Mello [UNIFESP]