Navegando por Palavras-chave "Mutagenicity"

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    Alterações citogenéticas em indivíduos que fazem uso de terapia antirretroviral, portadores ou não do vírus da Imunodeficiência Humana (HIV)
    (Universidade Federal de São Paulo, 2023-12-06) Alpire, Maria Esther Suárez [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/7233486130289112; Universidade Federal de São Paulo (UNIFESP)
    Alterações no DNA podem ser observadas através do teste do micronúcleo, uma importante ferramenta de biomonitoramento genotóxico. Este estudo utilizou células esfoliadas da mucosa bucal para avaliar in vivo portadores ou não do vírus da Imunodeficiência Humana (HIV) e que fazem uso de terapia antirretroviral (TARV), e as possíveis alterações no DNA. Em relação aos portadores de HIV, o estudo envolveu 39 crianças e adolescentes, com idade de 0 a 19 anos, em estudo comparativo (ou transversal), duplo­cego. Um total de 20 crianças portadoras de HIV em uso de terapia antirretroviral (TARV), e 19 crianças e adolescentes do grupo controle, pareados em relação ao sexo e idade foram inclusos. No estudo envolvendo adultos, foram examinados 37 indivíduos, sendo 17 do grupo profilaxia pré exposição (PrEP), que faziam uso de TARV, porém não eram portadores do vírus HIV, e 20 indivíduos do grupo controle, pareados por idade, sexo e hábitos, em estudo comparativo, duplo­cego. Os resultados demonstraram que no grupo HIV, as frequências de micronúcleos (MN) (p=0,05), células binucleadas (p=0,001) e, brotos nucleares (p=0,03); apresentaram aumentos em relação ao grupo controle. Nos parâmetros de citotoxicidade, houve aumento com diferença estatística na frequência de cariorrexe (p=0,05). Com relação ao grupo PrEP, os parâmetros de mutagenicidade também estavam aumentados, com diferença estatística significativa para as frequências de micronúcleos (p=0,0001); células binucleadas (p=0,001); e brotos nucleares (p=0,078). Nos parâmetros de citotoxicidade, houve aumento com diferença estatística na frequência de cariorrexe (p=0,001). Adicionalmente, foi realizado o índice de sistema de reparo, representado pela fórmula (CL+CR)/(MN+NB), onde ambos grupos de estudo apresentaram menor capacidade de reparo em relação ao grupo controle. Em suma, o uso contínuo destes antirretrovirais induzem danos citogenéticos e de citotoxicidade em células de mucosa oral nos indivíduos portadores independente da presença do vírus HIV.
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    Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium
    (Springer, 2014-11-01) Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Flavia Andressa Pidone [UNIFESP]; Jesus, Gustavo Protasio Pacheco de [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Gollucke, Andrea Pittelli Boiago [UNIFESP]; Aguiar, Odair [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of grape juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n=5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-grape juice group (Cd+GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd+GJ group received grape juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg(-1) day(-1)). Grape juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with grape juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd+GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that grape juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
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    Avaliação citogenética em células de mucosa oral de indivíduos infectados por SARS- CoV-2 (COVID-19)
    (Universidade Federal de São Paulo, 2022-10-19) Guedes-Pinto, Thiago [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/4223289411216225; Universidade Federal de São Paulo (UNIFESP)
    A COVID-19 pode progredir à insuficiência respiratória progressiva como consequência de dano alveolar, desencadeando a morte do indivíduo. O objetivo deste estudo foi avaliar o dano citogenético em células orais de pacientes com COVID-19 por meio do ensaio de micronúcleo. Um total de 11 pacientes com COVID-19 com média de idade de 40,7±9,3 anos (5 homens e 6 mulheres) foram inclusos neste estudo. Para o grupo controle, foram inclusos um total de 15 participantes não infectados pelo vírus SARS-CoV-2. A média de idade do referido grupo foi de 41,6±6,2 anos (5 homens e 10 mulheres). Os resultados mostraram diferenças estatisticamente significativas (p<0,05) nas frequências de células micronucleadas da mucosa bucal de pacientes com COVID-19. Além disso, um aumento estatisticamente significativo na cariólise e carriorexe (p <0,05) foi observado em pacientes com COVID-19 em comparação com o controle. Em suma, os resultados permitem concluir que o vírus SARS-CoV-2 pode induzir mutagênese e citotoxicidade em células orais.
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    Avaliação da citotoxicidade, genotoxicidade e mutagenicidade em ratos wistar expostos ao efluente lançado no emissário submarino da cidade de Santos/SP
    (Universidade Federal de São Paulo (UNIFESP), 2016-04-07) Silva, Victor Hugo Pereira da [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Nowadays, it has been demonstrated that wastewater released in continental or even marine coast is one of the most causes of pollution in the environment. Urban sewage is generated from residual water treatment process from domestic, medical, industrial, and rural anthropogenic activities. Due to chemical?physical process involved in this treatment, the sewage tends to concentrate compounds such as nitrates, ammonia and phosphate, heavy metals and organic trace compounds poorly degradable including PAH (polycyclic aromatic hydrocarbons) as well as pathogenic and non-pathogenic organisms. Nevertheless, the presence of persistent organic pollutants, nanocompouds, pharmaceutical compounds and personal care and disinfection by products know as emergent pollutants are expected as well. The interaction between these chemicals compounds is able to cause environmental effects such as reproductive, metabolic and genetic. The aim of this present study was to evaluate cytotoxicity, genotoxicity and mutagenicity in multiple organs of Wistar rats exposed to municipal effluents discharged by municipal outfall in Santos city. A total of 20 male Wistar rats were exposed to effluents by drinking water ad libitum at concentrations of 0, 10, 50, and 100 % for 30 days. The analysis for characterization of organic compounds was performed by high resolution liquid chromatography (UPLC) in negative mode. For analysis of histopathological changes, liver and kidney were analyzed, the comet assay was used to evaluate the genotoxic damage in blood, liver and kidney, and the challenge test was used for DNA repair evaluation in liver cells under exposure to 4-NQO and H2O2. To analyze micronuclei frequency histological slide stained with Feulgen and bone marrow smears stained with Giemsa were used to evaluate the mutagenicity. Microscopic analysis revealed severe lesions such as necrosis and hemorrhagic areas in liver and kidney from animals exposed to effluent at 50 and 100% concentration. DNA damage in peripheral blood, liver, and kidney cells were detected by comet assay at higher concentrations of effluent. Moreover, a decrease DNA repair capacity was also detected in liver cells. Significant statistical differences (p<0,05) for micronucleated cells from liver were noticed at 50% concentration of effluent. Taken together, our results demonstrate that municipal effluent is able to induce cytotoxicity and genotoxicity in multiple organs of Wistar rats.
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    Biomonitoramento citogenético em células da mucosa bucal e sangue periférico em pintores de automóveis
    (Universidade Federal de São Paulo, 2012-04-10) Silva, Victor Hugo Pereira da [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/4534973277501184; Universidade Federal de São Paulo (UNIFESP)
    Os pintores de automóveis estão ocupacionalmente expostos a uma gama de substâncias danosas provenientes não somente das tintas automotivas, mas também de solventes orgânicos, hidrocarbonetos aromáticos policíclicos, resinas plásticas e metais. Essa exposição prolongada pode estar relacionada ao aumento da incidência de doenças crônico-degenerativas inclusive o câncer. O objetivo do presente estudo foi avaliar a citotoxicidade e mutagenicidade por meio do teste do micronúcleo em células da mucosa jugal e genotoxicidade a partir do teste do cometa em leucócitos de sangue periférico em pintores de automóveis. Para o teste do micronúcleo, foi utilizado um total de 24 indivíduos expostos e 21 voluntários controle (indivíduos não-expostos). Para o ensaio do cometa, um total de 24 voluntários expostos e 19 voluntários controle foram avaliados. A análise de células bucais revelou que a frequência de micronúcleos em indivíduos expostos foi significativamente maior uma vez comparada ao controle (p<0,05). Porém, a citotoxicidade não foi diferente entre os grupos. O teste do cometa revelou um aumento estatisticamente significativo no momento da cauda dos cometas dos pintores de automóveis quando comparado ao grupo controle (p<0,05). Em suma, tais resultados sugerem que indivíduos ocupacionalmente expostos a tintas automotivas apresentam genotoxicidade e mutagenicidade em células de sangue periférico e mucosa bucal, respectivamente, sendo necessárias ações profiláticas que diminuam a exposição desses profissionais a esses produtos de risco.
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    Citogenotoxicidade e mutagenicidade da água do Sistema Estuarino de Santos-São Vicente
    (Universidade Federal de São Paulo (UNIFESP), 2019-11-25) Cardoso, Caroline Margonato [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/4298143669596994; Universidade Federal de São Paulo (UNIFESP)
    The Santos-São Vicente Estuarine System (SESS) is one of the most degraded estuaries in Brazil. The disorderly urbanization of the Baixada Santista Metropolitan Region (RMBS) added with the inefficiency of regulatory mechanisms and basic sanitation, poverty, poor housing conditions, and environmental pollution make this region suitable for environmental monitoring studies. Thus, the aim of this study was to evaluate the water-induced cytogenotoxicity and mutagenicity of SSES collected at the points described as most contaminated (São Vicente and Piaçaguera Channels) using Wistar rats as an experimental model and Centropomus undecimalis fish. For this purpose, surface water was collected São Vicente Channel (SVC) and Piaçaguera Channel (PIC) of SESS and non-essential metals and metalloids (Cd, Pb, Hg and As) were quantified. Adult male Wistar rats were divided into four groups (n = 5) and exposed for 5 days: a) negative control received filtered water, b) saline control received saline water, c) experimental group received estuarine water from PIC and d) experimental group received estuarine water from SVC. Blood and liver were used for comet assay; bone marrow was used to perform the micronucleus test; histopathological, immunohistochemical analyzes (TNF-α, ki67 and 8-OHdG) and realtime PCR of caspase-3 were performed in the liver. The results revealed that rats exposed to water from SESS showed biological responses associated with genotoxicity, mutagenicity and cytotoxicity. Juvenile fish (n = 6) from C. undecimalis were collected from the SVC and compared to fish (n = 10) obtained from a pisciculture laboratory (control group). Micronucleus tests and nuclear abnormalities were performed with blood, and quantification of lipid peroxidation and DNA damage were performed with gills and livers of fish. Erythrocyte nuclear abnormalities in blood and lipid peroxidation in gills were higher in fish from SESS. In conclusion, SESS is capable of both mutagenic and cytotoxic activities in murine and fish, both having similar responses to environmental contaminants.
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    Genotoxicity and cytotoxicity induced by municipal effluent in multiple organs of Wistar rats
    (Springer, 2014-11-01) Silva, Victor Hugo Pereira da [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Flavia Andressa Pidone [UNIFESP]; Cesar, Augusto [UNIFESP]; Pereira, Camilo Dias Seabra [UNIFESP]; Silva, Marcelo Jose Dias; Vilegas, Wagner; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); São Paulo State Univ
    The aim of this study was to evaluate cytotoxicity and genotoxicity in multiple organs of rats induced by municipal effluent released by submarine outfall in city of Santos. A total of 20 male Wistar rats were exposed to effluents by drinking water ad libitum at concentrations of 0, 10, 50, and 100% for 30 days. Microscopic analysis revealed severe lesions such as necrosis and hemorrhagic areas in liver and kidney from animals exposed to effluent at 50 and 100% concentration. DNA damage in peripheral blood, liver, and kidney cells were detected by comet assay at higher concentrations of effluent. Moreover, a decrease DNA repair capacity was detected in liver cells. Significant statistical differences (p<0.05) for micronucleated cells from liver were noticed at 50% concentration of effluent. Taken together, our results demonstrate that municipal effluent is able to induce cytotoxicity and genotoxicity in multiple organs of Wistar rats.
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    Genotoxicity, mutagenicity and cytotoxicity of carotenoids extracted from ionic liquid in multiples organs of Wistar rats
    (Elsevier Gmbh, Urban & Fischer Verlag, 2016) Larangeira, Paula Martins [UNIFESP]; Rosso, Veridiana Vera de [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]
    The ionic liquid or melted salt 1-Butyl-3-methylimidazolium is an alternative process to extract natural pigments, such as carotenoids. Lycopene represents 80-90% of total of carotenoids presents in tomatoes and it has been widely studied due its potent antioxidant action. The aim of this study was to evaluate genotoxicity, mutagenicity and cytotoxicity of carotenoids extracted from ionic liquid using experimental model in vivo. For this purpose, a total of 20 male Wistar rats were distributed into four groups (n = 5), as follows: control group