Navegando por Palavras-chave "Micronucleus test"

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    Avaliação odontológica e danos em dna nas mucopolissacaridoses i, ii e vi
    (Universidade Federal de São Paulo (UNIFESP), 2013-03-27) Guilheiro, Joice Marques [UNIFESP]; D'Almeida, Vânia [UNIFESP]; http://lattes.cnpq.br/7220411418339421; http://lattes.cnpq.br/9906565818359453; Universidade Federal de São Paulo (UNIFESP)
    Background: The goal of the study was to investigate through the comet assay in peripheral blood and analyze in cells of oral mucosa through the micronucleus test damages in DNA leads by GAGs accumulation in patients diagnosed with mucopolysaccharidosis I, II or VI. Moreover, anamnesis and a clinical examination in Dentistry were also performed, and the clinical findings in Dentistry were compared with described by literature. Methods: Twelve patients, treated in Centro de Referência de Erros Inatos do Metabolismo (CREIM - UNIFESP), diagnosed with mucopolysaccharidosis I, II or VI and in enzyme replacement therapy treatment were include in the study. The control group consisted of healthy subjects, with the same age and gender to the patients. For the DNA damage, the comet assay and micronucleus test were performed. An anamnesis and clinical examinations were evaluated for oral manifestations in MPS. The comet assay in peripheral blood was used to detect DNA damages and analyze in cells of oral mucosa through micronucleus to morphological analize and detect cell death parameters. An anamnesis and clinical examinations were evaluated for oral manifestations in MPS. Results: The mean of age of diagnosis for MPS II was 55.75±26.98 months, for MPS VI, 45.5±27.87 months and, for MPS I, 26.75±31.42 months. Significant clinical findings were present in MPS VI (5.25±1.5), followed by MPS I (2.25±1.71), and MPS II (1.5±1.91). The treatment time was 25±23.13 months for MPS I, 36.25±24.5 months for MPS II and, 42±28.56 months for MPS I. The genotoxicity values (comet assay) showed high results in MPS patients, comparing to control group (0.7±1.2). The result for MPS I was 4.7±1.4, in MPS II (higher value), 5.3±1.3 and, 3.2±1.4 for MPS VI. Micronucleus test mean values found was 0.2±0.07 for MPS II, 0.02±0.07 for MPS I and 0.07±0.01 for MPS VI. For cell death (pyknosis, karyorrhexis and karyolysis), the MPS VI had the higher score (34.2±13.4), followed by MPS II (23.7±11.3), and MPS I (20.6±8.5). Conclusions:Our results suggest that genotoxicity and cytotoxicity were more evident in mucosa bucal and peripheral blood from MPS patients than control subjects. Concerning the three types of MPS, the oral findings were evident, mainly in MPS VI patients which, in our sample, begun enzyme replacement therapy more lately than the others MPS.
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    Biomonitoramento citogenético em células da mucosa bucal e sangue periférico em pintores de automóveis
    (Universidade Federal de São Paulo, 2012-04-10) Silva, Victor Hugo Pereira da [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/4534973277501184; Universidade Federal de São Paulo (UNIFESP)
    Os pintores de automóveis estão ocupacionalmente expostos a uma gama de substâncias danosas provenientes não somente das tintas automotivas, mas também de solventes orgânicos, hidrocarbonetos aromáticos policíclicos, resinas plásticas e metais. Essa exposição prolongada pode estar relacionada ao aumento da incidência de doenças crônico-degenerativas inclusive o câncer. O objetivo do presente estudo foi avaliar a citotoxicidade e mutagenicidade por meio do teste do micronúcleo em células da mucosa jugal e genotoxicidade a partir do teste do cometa em leucócitos de sangue periférico em pintores de automóveis. Para o teste do micronúcleo, foi utilizado um total de 24 indivíduos expostos e 21 voluntários controle (indivíduos não-expostos). Para o ensaio do cometa, um total de 24 voluntários expostos e 19 voluntários controle foram avaliados. A análise de células bucais revelou que a frequência de micronúcleos em indivíduos expostos foi significativamente maior uma vez comparada ao controle (p<0,05). Porém, a citotoxicidade não foi diferente entre os grupos. O teste do cometa revelou um aumento estatisticamente significativo no momento da cauda dos cometas dos pintores de automóveis quando comparado ao grupo controle (p<0,05). Em suma, tais resultados sugerem que indivíduos ocupacionalmente expostos a tintas automotivas apresentam genotoxicidade e mutagenicidade em células de sangue periférico e mucosa bucal, respectivamente, sendo necessárias ações profiláticas que diminuam a exposição desses profissionais a esses produtos de risco.
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    Biomonitoramento citogenético em células de mucosa oral de estivadores portuários
    (Universidade Federal de São Paulo, 2022-07-20) Campos, Katyane Negreiros [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/7991738144134795; Universidade Federal de São Paulo (UNIFESP)
    O ambiente de trabalho portuário apresenta uma série de riscos aos trabalhadores, sejam de natureza física, química e/ou biológica. Em âmbito mundial, tem se encontrado forte relação entre a exposição a agentes mutagênicos e o desenvolvimento de diversas doenças crônico-degenerativas, tais como câncer. O objetivo deste estudo foi avaliar possíveis efeitos mutagênicos e citotóxicos em células esfoliadas da mucosa bucal de estivadores do Porto de Santos. Para isso, foram coletadas amostras de 26 estivadores portuários e 25 indivíduos para o grupo controle, sendo todos os voluntários não fumantes. A análise das células, foi realizada com auxílio da microscopia de luz, e coloração de Feulgen/Fast-Green. No total, foram observadas 2000 mil células por voluntário. Diferenças estatisticamente significativas (p<0,05) na frequência de micronúcleos foram detectadas nos estivadores portuários, quando comparados ao grupo controle. Entretanto, não foram encontradas alterações significativas (p>0,05) de parâmetros relacionados à citotoxicidade, tais como picnose, cariólise e carriorrexe, entre os grupos estudados. Em suma, nossos resultados indicam que os estivadores constituem um grupo de risco devido ao aumento da mutagênicidade encontrado nas células da mucosa bucal.
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    Biomonitoring of oral epithelial cells in smokers and non-smokers submitted to panoramic X-ray: comparison between buccal mucosa and lateral border of the tongue
    (Springer, 2010-12-01) Angelieri, Fernanda; Goncalves Moleirinho, Tatiana de Cassia; Carlin, Viviane [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); São Paulo Metodista Univ
    The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers submitted to dental X-ray using two anatomic sites: buccal mucosa and lateral border of the tongue. A total of 15 heavy smokers and 17 non-smokers were submitted to panoramic dental radiography for orthodontic reasons. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative, and dropped in clean slides which were checked for the above nuclear phenotypes. the results pointed out no significant statistically differences (p > 0.05) of micronucleated oral mucosa cells before versus after X-ray exposure for both oral sites evaluated either to smokers or to non-smokers. X-ray exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis, and karyolysis for two groups evaluated. Nevertheless, the most pronunciated effects were found to lateral border of the tongue of smokers. in summary, these data indicate that panoramic X-ray is able to induce cellular death in oral mucosa cells. It seems that lateral border of the tongue is more sensitive site to cytotoxic insult induced by ionizing radiation combined with continuous cigarette smoke exposure.
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    Cellular death but not genetic damage in oral mucosa cells after exposure to digital lateral radiography
    (Springer, 2011-06-01) Ribeiro, Daniel Araki [UNIFESP]; Sannomiya, Eduardo Kazuo; Pozzi, Renan [UNIFESP]; Miranda, Sandra Regina [UNIFESP]; Angelieri, Fernanda; Universidade Federal de São Paulo (UNIFESP); São Paulo Methodist Univ
    The aim of the present study was to evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from individuals following digital lateral radiography. A total of 30 healthy patients (15 men and 15 women) indicated to the orthodontic therapy were submitted to digital lateral X-ray. Exfoliated oral mucosa cells were collected immediately before the X-ray exposure and after 10 days. the results pointed out no significant statistically differences (p > 0.05) of micronucleated oral mucosa cells. On the other hand, X-ray was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis, and karyolysis. in summary, these data indicate that exposure to digital lateral radiography may not be a factor that induced chromosomal damage, but it is able to promote cytotoxicity.
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    Chromosome breakage and cellular death are induced in oral epithelial cells of hairdressers: a preliminary study
    (Informa Healthcare, 2013-02-01) Carlin, Viviane [UNIFESP]; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Miranda, Sandra Regina [UNIFESP]; Noguti, Juliana [UNIFESP]; Silva, Victor Hugo Pereira da [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of the present study was to comparatively evaluate genomic damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from hairdressers using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 28 hairdressers and 30 health controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from the cheek and lateral border of the tongue mechanically exfoliated, placed in fixative and dropped in clean slides that were checked for the previously mentioned nuclear phenotypes. the results pointed out statistically significant differences ( p < 0.05) of micronucleated oral mucosa cells from hairdressers in the lateral border of the tongue. Exposure to hair dyes caused an increase of other nuclear alterations closely related to cytotoxicity, such as karrhyorexis, pyknosis and karyolysis in both the oral sites evaluated. in summary, these data indicate that hairdressers are occupationally exposed to agents that are genotoxic and cytotoxic. It seems that the lateral border of the tongue is a more sensitive site to the genotoxic and cytotoxic effects of hair dyes.
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    Chromosome damage and cytotoxicity in oral mucosa cells after 2 months of exposure to anabolic steroids (decadurabolin and winstrol) in weight lifting
    (Elsevier B.V., 2010-12-01) Martins, Renato de Almeida [UNIFESP]; Gomes, Guilherme Augusto da Silva [UNIFESP]; Aguiar, Odair [UNIFESP]; Medalha, Carla Christina [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of the present study was to evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated buccal mucosa cells from anabolic steroid users after 2 months of exposure. Two experimental groups consisting of 15 adult males who practise weight lifting and are anabolic steroid users or 15 adult males who practise weight lifting, but are non-anabolic steroid users, were recruited. in addition, 20 sedentary males, who do not practise any physical activity regularly, were matched by age with experimental groups. No significant statistical differences (p > 0.05) were noticed in individuals who practise physical activity only. On the other hand, an increase of micronucleated cells (MNCs) in anabolic steroid (decadurabulin and Winstrol) users was observed. Regarding cytotoxic parameters, the same observation has occurred, that is, significant statistical differences (p < 0.05) were noticed in the group exposed to anabolic steroids when compared with other controls, as depicted by high frequencies of pyknosis, karyolysis and karyorrhexis. Taken together, our results suggest that genomic instability and cytotoxicity are induced by anabolic steroid administration in oral mucosa cells as assessed by the micronucleus test. (C) 2010 Elsevier Inc. All rights reserved.
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    Cytogenetic Biomonitoring in Buccal Mucosal Cells from Municipal Solid Waste Collectors
    (Int Inst Anticancer Research, 2017) Andrade, Mariana Carvalho; Santos, Jean Nunes dos; Cury, Patricia Ramos; Souza, Ana Carolina Flygare [UNIFESP]; Claudio, Samuel Rangel [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]
    Waste collectors collect, transport, and process the garbage produced by people living in the city. Nowadays, this activity requires special attention due to the environmental impact of garbage and its potential consequences on human health. The aim of this study was to evaluate potential cytotoxic and mutagenic effects of garbage collection on waste collectors. For this purpose, a total of 47 male waste collectors aged from 24 to 53 years were included in the experimental group. A total of 30 men matched by age were used as the control group. Cytotoxicity and mutagenicity were analyzed by micronucleus test in buccal mucosaI cells. No statistically significant difference (p>0.05) in the frequency of micronuclei was detected in the waste collectors when compared to controls. Nevertheless, higher frequencies of karyolysis and pyknosis (p<0.05) were detected in buccal mucosaI cells from waste collectors when compared to matched controls. Taken together, our results indicate that waste collectors comprise an at-risk group as a result of increased cytotoxicity apparent from buccal mucosa cells.
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    Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning
    (E H Angle Education Research Foundation, Inc, 2012-07-01) Lorenzoni, Diego Coelho; Fracalossi, Ana Carolina Cuzzuol [UNIFESP]; Carlin, Viviane [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Sant' Anna, Eduardo Franzotti; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Federal de São Paulo (UNIFESP)
    Objectives: To evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from children undergoing orthodontic radiographs.Materials and Methods: A total of 25 healthy children undergoing orthodontic therapy partook in a complete set of orthodontic radiographs (lateral cephalographic, posteroanterior cephalographic, panoramic, full periapical exam, and bitewing). the micronucleus test in the buccal exfoliated cells was applied. the paired-samples t-test and the Wilcoxon test were used to compare the frequencies of alterations before and after X-ray exposure.Results: We found no statistically significant differences (P > .05) between micronucleated buccal mucosa cells before and after exposure to radiation. However, radiation did cause other nuclear alterations closely related to cytotoxicity (P = .007).Conclusion: According to the micronucleus test, the complete set of radiographs requested in the orthodontic planning may not be a factor that induces chromosomal damage, but it is able to promote cytotoxicity. (Angle Orthod. 2012;82:585-590.)
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    Cytogenetic biomonitoring in patients exposed to dental X-rays: comparison between adults and children
    (British Inst Radiology, 2008-10-01) Ribeiro, Daniel Araki [UNIFESP]; Oliveira, Gabriela Rodrigues de; Castro, Glaucia Monteiro de [UNIFESP]; Angelieri, Fernanda; Universidade Federal de São Paulo (UNIFESP); Univ Metodista São Paulo
    Objectives: the aim of the present study was to comparatively evaluate the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) of exfoliated buccal mucosa cells from children and adults following dental X-ray exposure.Methods: A total of 17 adults and 17 children submitted to panoramic dental radiography were included in this study.Results: the results indicated no statistically significant differences (P > 0.05) in children's micronucleated oral mucosa cells before and after dental X-ray exposure. in the same way, no mutagenic effects were observed in adults following X-ray exposure. On the other hand, X-rays increased other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis in both groups. the comparative analysis between children and adults demonstrated no statistically significant differences in micronucleus frequency or (P > 0.05).Conclusions: in summary, these data indicate that dental paroramic radiography may not be a factor that includes chromosomal damage, but it is able to promote cytotoxicity. It seems that children are not more susceptible to the noxious activities induced by X-rays when compared with adults.
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    Cytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays
    (Springer, 2008-07-01) Ribeiro, Daniel Araki [UNIFESP]; Angelieri, Fernanda; Universidade Federal de São Paulo (UNIFESP); Univ Metodista São Paulo
    Purpose. Although it has been clearly demonstrated that X-rays play a key role in diagnosing medical and dental problems, this type of ionizing radiation is also able to induce noxious activities, such as genetic damage. the aim of the present study was to evaluate DNA damage (micronucleus) and cellular death in exfoliated buccal mucosa cells from healthy individuals (smokers and nonsmokers) following dental X-ray exposure.Material and methods. A total of 39 healthy people who had submitted to panoramic dental radiography were included in the study: 9 smokers and 30 nonsmokers.Results. the results indicated no significant statistically differences (P > 0.05) in micronucleated oral mucosa cells before and after dental X-ray exposure. On the other hand, X-ray exposure did increase other nuclear alterations closely related to cytotoxicity, such as karyorrhexis, pyknosis, and karyolysis. It seems that cigarette smoke did not affect X-ray outcomes induced in buccal cells.Conclusion. These data indicate that dental panoramic radiography may not induce chromosomal damage, but it is able to promote cytotoxicity. Because cellular death is considered a prime mechanism in nongenotoxic mechanisms of carcinogenesis, dental X-ray should be used only when necessary.
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    Cytogenetic biomonitoring of peripheral blood and oral mucosa cells from car painters
    (Informa Healthcare, 2012-09-01) Silva, Victor Hugo Pereira da [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; Spadari-Bratfisch, Regina Celia [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 mu L from peripheral blood was collected for the single cell gel (comet) assay. the results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. in addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. in summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.
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    Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro
    (Springer, 2012-06-01) Carlin, Viviane [UNIFESP]; Matsumoto, Mariza A.; Saraiva, Patricia P.; Artioli, Andre; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); USC
    The aim of the present study was to comparatively evaluate DNA damage and cellular death in cells exposed to various commercially available mouthrinses: ListerineA (R) CepacolA (R), Plax alcohol freeA (R), PeriogardA (R), and Plax WhiteningA (R). A total of 75 volunteers were included in the search distributed into five groups containing 15 people each for in vivo study. Exfoliated buccal mucosa cells were collected immediately before mouthrinse exposure and after 2 weeks. Furthermore, blood samples were obtained from three healthy donors for in vitro study. the micronucleus test was used to evaluate mutagenicity and cytotoxicity in vivo. the single-cell gel (comet) assay was used to determine DNA damage in vitro. After 2 weeks exposure, PeriogardA (R) showed 1.8% of micronucleated cells with significant statistical differences ( < 0.05) compared to before exposure (0.27%). Plax WhiteningA (R) presented high tail moment value (4.5) when compared to negative control (0.6). the addition of all mouthrinses to cells incubated with methyl methanesulfonate did not alter the number of strand breaks in the genetic material. ListerineA (R) was able to reduce genetic damage induced by hydrogen peroxide because a decrease of tail moment was noticed. the results of the present study suggest that PeriogardA (R) and Plax WhiteningA (R) can induce genetic damage, whereas ListerineA (R) is an antioxidant agent. Since DNA damage is considered to be prime mechanism during chemical carcinogenesis, these data may be relevant in risk assessment for protecting human health and preventing carcinogenesis.
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    Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis
    (Wiley-Blackwell, 2010-09-01) Matsumoto, Mariza Akemi; Castanho, Juliane; Kawakami, Roberto Yoshio; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    Objectives:The aim of this study was to evaluate comparatively the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from chronic denture stomatitis patients and healthy controls.Background:Over the course of ageing, individuals may develop many diseases such as denture stomatitis.Material and methods:A total of 23 chronic denture stomatitis patients and 23 controls presenting good oral conditions were included in this study. Individuals had epithelial cells mechanically exfoliated, placed in fixative and placed on clean slides, which were checked for nuclear phenotypes.Results:The results indicated no statistically significant differences (p > 0.05) of micronucleated oral mucosa cells from chronic denture stomatitis patients when compared to healthy controls. Nevertheless, chronic denture stomatitis was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis as depicted by significant differences (p < 0.05) between groups. No interaction was observed between smoking and chronic denture stomatitis.Conclusion:In summary, these data indicated that chronic denture stomatitis was able to induce cytotoxic effects as assessed by a micronucleus test.
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    Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo
    (Elsevier B.V., 2007-05-01) Azevedo, Luciana; Lima, Patricia Lepage Alves de; Gomes, Jose Carlos; Stringheta, Paulo Cesar; Ribeiro, Daniel Araki [UNIFESP]; Salvadori, Daisy Maria Favero; Universidade de São Paulo (USP); Universidade Federal de Viçosa (UFV); Universidade Federal de São Paulo (UNIFESP)
    Anthocyanins are the largest group of water-soluble pigments in the plant kingdom. A number of studies have demonstrated that anthocyanins present antioxidant capacity and show inhibitory effects on the growth of some cancer cells. Thus, the goal of this study was to evaluate both the antimutagenicity/antigenotoxicity and mutagenicity/genotoxicity of aqueous extract obtained from the Solanum melanogena, a possible novel source of anthocyanin, and its main purified anthocyanin extract (delphinidin), using the single cell (comet) assay and micronucleus test. Pretreatment with higher doses of the purified anthocyanin (10 and 20 mg/kg b.w.) led to a statistically significant reduction (p < 0.05) in the frequency of micronuclei in polychromatic erythrocytes induced by cyclophosphamide. the pattern of reduction ranged from 48% to 57% independent of concentration. No apparent: genotoxicity and mutagenicity was found for either the anthocyanin or delphinidin extracts. Taken together, these results suggest that mice pre-treated with specific compounds present in anthocyanins (delphinidin) displayed a lower incidence of mutations induced by cyclophosphamide. This finding emphasizes the potential of natural colorants to prevent mutations and also the applicability of genotoxic evaluation for improving health. Furthermore, the results presented here could be an additional argument to support the use of anthocyanins in the diet. (c) 2006 Published by Elsevier B.V.
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    Dragon's blood Croton palanostigma induces genotoxic effects in mice
    (Elsevier B.V., 2013-05-20) Maistro, Edson Luis; Ganthous, Giulia; Machado, Marina da Silva; Zermiani, Tailyn; Andrade, Sergio Faloni de; Pires Rosa, Paulo Cesar [UNIFESP]; Perazzo, Fabio Ferreira [UNIFESP]; Univ Estadual Paulista UNESP; Univ Vale Itajai UNIVALI; Universidade Federal de São Paulo (UNIFESP)
    Ethnopharmacological relevance: Dragon's blood is a dark-red sap produced by species from the genus Croton (Euphorbiaceae), which has been used as a famous traditional medicine since ancient times in many countries, with scarce data about its safe use in humans. in this research, we studied genotoxicity and clastogenicity of Croton palanostigma sap using the comet assay and micronucleus test in cells of mice submitted to acute treatment.Material and methods: HPLC analysis was performed to identify the main components of the sap. the sap was administered by oral gavage at doses of 300 mg/kg, 1000 mg/kg and 2000 mg/kg. for the analysis, the comet assay was performed on the leukocytes and liver cells collected 24 h after treatment, and the micronucleus test (MN) on bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio).Results and conclusion: the alkaloid taspine was the main compound indentified in the crude sap of Croton palanostigma. the results of the genotoxicity assessment show that all sap doses tested produced genotoxic effects in leukocytes and liver cells and also produced clastogenic/aneugenic effects in bone marrow cells of mice at the two higher doses tested. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution in the use of Croton palanostigma sap by humans considering its risk of carcinogenesis. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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    Evaluation of the genotoxicity of Euterpe oleraceae Mart. (Arecaceae) fruit oil (acai), in mammalian cells in vivo
    (Pergamon-Elsevier Science Ltd, 2016) Marques, E. S.; Froder, J. G.; Carvalho, J. C. T.; Rosa, P. C. P.; Perazzo, F. F. [UNIFESP]; Maistro, E. L.
    E. oleracea is a tropical plant from the Amazon region, with its fruit used for food, and traditionally, as an antioxidant, anti-inflammatory, hypocholesterolemic, for atherosclerotic disease, and has anticancer properties. The oil of the fruit has antidiarrheic, anti-inflammatory and antinociceptive activities, but without genotoxicity evaluation. Therefore, the aim of this study was to evaluate the genotoxic potential of E. oleracea fruit oil (EOO), in rat cells. Male Wistar rats were treated with EOO by gavage at doses of 30, 100 and 300 mg/kg, for 14 days, within a 24 h interval. The DNA damage in the leukocytes, liver, bone marrow and testicular cells, was assessed by the comet assay, and the clastogenic/aneugenic effects in the bone marrow cells, by the micronucleus test. Our phytochemicals characterization of the EOO showed the presence of vanillic, palmitic, gamma-linolenic, linoleic, oleic, cinnamic, caffeic, protocatechuic, ferulic, syringic acids, and flavonoids quercetin and kaempferol rutinoside as the main constituents. Both cytogenetic tests performed showed that EOO presented no significant genotoxic effects in the analyzed cells, at the three tested doses. These results indicate that, under our experimental conditions, E. oleracea fruit oil did not reveal genetic toxicity in rat cells. (C) 2016 Published by Elsevier Ltd.
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    First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells
    (Funpec-Editora, 2017) Marques, E. S.; Tsuboy, M. S. F.; Carvalho, J. C. T.; Rosa, P. C. P.; Perazzo, F. F. [UNIFESP]; Gaivao, I. O. M.; Maistro, E. L.
    Euterpe oleracea Mart., popularly known as "acai", is a tropical fruit from the Amazon region where it has considerable economic importance. Acai has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and antigenotoxic effects of E. oleracea fruit oil (EOO) in cultured human lymphocytes (non-metabolizing cells) and HepG2 cell line (human hepatoma) (metabolizing cells) by using MTT, comet, and micronucleus assays. A wide range of EOO concentrations was tested with a preliminary MTT assay, which allowed selecting five concentrations for comet and micronucleus assays: 2.5, 10, 100, 500, and 1000 mu g/mL. The results showed that none of the EOO tested concentrations presented cytotoxic effects. The genotoxic assessment revealed an absence of significant DNA and chromosome damage in human lymphocytes and HepG2 cells but did not show chemoprotection against the DNA damage induced by methyl methanesulfonate and benzo[a] pyrene, used as DNA-damaging agents.
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    Genotoxic assessment of Rubus imperialis (Rosaceae) extract in vivo and its potential chemoprevention against cyclophosphamide-induced DNA damage
    (Elsevier B.V., 2014-05-14) Costa Rodrigues Alves, Ana Beatriz; Santos, Rafaella Souza dos; Calil, Susana de Santana; Niero, Rivaldo; Lopes, Jhonny da Silva; Perazzo, Fabio E. [UNIFESP]; Pires Rosa, Paulo Cesar [UNIFESP]; Andrade, Sergio Faloni; Cechinel-Filho, Valdir; Maistro, Edson Luis; Univ Estadual Paulista; Univ Vale Itajai; Universidade Federal de São Paulo (UNIFESP)
    Ethnopharmacological relevance: Rubus imperialis Cham. Schl. (Rosaceae) is frequently used in traditional medicine as hypoglycemic, antinociceptive and antiviral remedy.Materials and methods: Swiss albino mice were distributed in eight groups for acute treatment with Rubus imperialis extract (24 h). the extract doses selected were 50, 250 and 500 mg/kg b.w. administered by gavage alone or plus to CPA (50 mg/kg b.w.) administered by intraperitoneal injection. Control groups were treated in a similar way. Analyses were performed using the comet assay, on leukocytes (collected 4 and 24 h after treatment) and liver (collected 24 h after treatment), and using the micronucleus test (MN) in bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio).Results and conclusion: the main compounds identified in the Rubus imperialis extract were saponins and steroidal compounds, with niga-ichigoside and tormentic acid being the major compounds. Tested doses of Rubus imperialis extract showed no genotoxic effects on leukocytes from peripheral blood or liver cells by the comet assay. However, the MN test showed an increase in the frequency of micronucleated cells at the two higher doses tested, indicating that this extract has clastogenic/aneugenic effects on bone marrow cells at higher doses. On the other hand, for all cells evaluated, the three tested doses of the Rubus imperialis extract promoted inhibition of DNA damage induced by CPA. Despite the chemoprevention observed, the clastogenicity/aneugenicity observed suggested caution about either continuous or high-dose usage of Rubus imperiale aerial parts extract by humans. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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    Genotoxicity assessment of Garcinia achachairu Rusby (Clusiaceae) extract in mammalian cells in vivo
    (Elsevier B.V., 2012-07-13) Marques, Eduardo de Souza; Silva, Suellen; Niero, Rivaldo; Andrade, Sérgio Faloni de; Rosa, Paulo César Pires [UNIFESP]; Perazzo, Fábio Ferreira [UNIFESP]; Maistro, Edson Luis; Univ Estadual Paulista; Universidade de São Paulo (USP); Univ Vale Itajai; Universidade Federal de São Paulo (UNIFESP)
    Ethnopharmacological relevance: Garcinia achachairu Rusby (Clusiaceae) is popularly known as achachairu, and is used in Bolivian folk medicine for its healing, digestive, and laxative properties, and in the treatment of gastritis, rheumatism and inflammation. Despite its widespread therapeutic use, there is a lack of data regarding its in vivo genotoxic effects. Therefore, in this study, we used the comet assay and the micronucleus test, respectively, to evaluate the possible genotoxic and clastogenic effects of Garcinia achachairu seed extract (GAE) on different cells of mice.Material and methods: the GAE was administered by oral gavage at doses of 500, 1000 and 2000 mg/kg. for the analysis, the comet assay was performed on the leukocytes (collected 4 and 24 h after treatment), liver, bone marrow and testicular cells (collected 24 h after treatment), and the micronucleus test (MN) on bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio).Results and conclusion: the results showed that GAE did not induce significant DNA damage in leukocytes (4 h and 24 h samples), liver, bone marrow and testicular cells (24 h samples). GAE also did not show any significant increase in micronucleated polychromatic erythrocytes (MNPCEs) at the three tested doses. the PCE/NCE ratio indicated no cytotoxicity. Under our experimental conditions, the data obtained suggest that a single oral administration of G. achachairu extract does not cause genotoxicity and clastogenicity in different cells of mice. (C) 2012 Elsevier Ireland Ltd. All rights reserved.